Mutagenetix > Incidental Mutation

Incidental Mutation 'R5178:Vmn2r95'

399518

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r95

Ensembl Gene

ENSMUSG00000091631

Gene Name

vomeronasal 2, receptor 95

Synonyms

MMRRC Submission

042758-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.067) question?

Stock #

R5178 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

18644366-18672586 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 18660337 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 250 (T250A)

Ref Sequence

ENSEMBL: ENSMUSP00000156383 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000166327] [ENSMUST00000232090] [ENSMUST00000232464]

AlphaFold

A0A338P6T0

Predicted Effect

probably benign
Transcript: ENSMUST00000166327
AA Change: T250A

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000126106
Gene: ENSMUSG00000091631
AA Change: T250A

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
Pfam:ANF_receptor	82	462	1.8e-35	PFAM
Pfam:NCD3G	509	562	3.2e-20	PFAM
Pfam:7tm_3	594	830	3.2e-51	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000232090
AA Change: T250A

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

Predicted Effect

probably benign
Transcript: ENSMUST00000232464
AA Change: T250A

PolyPhen 2 Score 0.009 (Sensitivity: 0.96; Specificity: 0.77)

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.3%
20x: 95.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abt1	G	T	13: 23,606,819 (GRCm39)	A94E	possibly damaging	Het
Carmil2	G	T	8: 106,423,521 (GRCm39)	G1207V	probably damaging	Het
Cndp2	A	T	18: 84,693,153 (GRCm39)	N202K	probably benign	Het
Cyp2c66	A	G	19: 39,159,473 (GRCm39)	Y308C	probably damaging	Het
Dhx29	T	G	13: 113,069,134 (GRCm39)	S155A	possibly damaging	Het
Dnah7b	T	G	1: 46,397,376 (GRCm39)	F3936V	possibly damaging	Het
Dsg1b	G	A	18: 20,530,560 (GRCm39)	G405E	probably damaging	Het
Exo5	A	T	4: 120,778,734 (GRCm39)		probably null	Het
Fam171b	T	C	2: 83,710,331 (GRCm39)	F668L	probably damaging	Het
Gemin5	A	T	11: 58,037,344 (GRCm39)	S624T	probably benign	Het
Gpc2	C	A	5: 138,273,867 (GRCm39)	V444L	possibly damaging	Het
Hephl1	T	A	9: 14,997,468 (GRCm39)	K399N	probably damaging	Het
Lair1	G	A	7: 4,013,488 (GRCm39)	T82I	possibly damaging	Het
Lhx1	G	T	11: 84,411,214 (GRCm39)	A155E	possibly damaging	Het
Maip1	G	A	1: 57,454,849 (GRCm39)	D278N	probably benign	Het
Mcm4	T	A	16: 15,453,167 (GRCm39)	D174V	probably benign	Het
Mcph1	T	A	8: 18,657,342 (GRCm39)	D60E	probably damaging	Het
Mmp17	T	A	5: 129,672,122 (GRCm39)	W132R	probably damaging	Het
Or4p7	C	T	2: 88,221,819 (GRCm39)	T76I	possibly damaging	Het
Or7e174	T	A	9: 20,012,488 (GRCm39)	C144*	probably null	Het
Pds5a	T	C	5: 65,821,218 (GRCm39)	T169A	probably damaging	Het
Pfkm	A	G	15: 98,029,396 (GRCm39)	N697D	probably benign	Het
Pira1	T	A	7: 3,742,396 (GRCm39)	K44*	probably null	Het
Ppwd1	A	T	13: 104,356,943 (GRCm39)	S191T	probably benign	Het
Ranbp2	T	A	10: 58,312,607 (GRCm39)	M1109K	probably benign	Het
Robo4	T	C	9: 37,319,256 (GRCm39)	W535R	probably damaging	Het
Rsf1	A	G	7: 97,311,079 (GRCm39)	D603G	possibly damaging	Het
Slc22a26	T	C	19: 7,767,540 (GRCm39)	T289A	possibly damaging	Het
Sp2	A	G	11: 96,846,664 (GRCm39)	F554L	probably benign	Het
Sp4	A	G	12: 118,225,624 (GRCm39)	V580A	possibly damaging	Het
Tbc1d19	T	A	5: 54,046,667 (GRCm39)	S413T	possibly damaging	Het
Tecta	T	C	9: 42,286,481 (GRCm39)	D725G	probably damaging	Het
Tmem200a	T	A	10: 25,870,277 (GRCm39)	Q4H	probably benign	Het
Tpx2	T	A	2: 152,717,469 (GRCm39)	I122N	probably benign	Het
Trgc4	A	G	13: 19,528,932 (GRCm39)		probably benign	Het
Ube4b	T	C	4: 149,427,449 (GRCm39)	M900V	probably damaging	Het
Vmn1r87	G	T	7: 12,865,792 (GRCm39)	A165E	possibly damaging	Het
Wasf1	G	A	10: 40,813,672 (GRCm39)	R518H	unknown	Het
Zfhx2	A	T	14: 55,312,232 (GRCm39)	F154Y	probably benign	Het
Zfp729a	A	G	13: 67,785,764 (GRCm39)		probably null	Het
Zfp976	A	G	7: 42,261,925 (GRCm39)		probably null	Het

Other mutations in Vmn2r95

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01024:Vmn2r95	APN	17	18,672,590 (GRCm39)	utr 3 prime	probably benign
IGL01479:Vmn2r95	APN	17	18,664,124 (GRCm39)	missense	probably damaging	1.00
IGL01890:Vmn2r95	APN	17	18,671,737 (GRCm39)	missense	probably damaging	1.00
IGL01986:Vmn2r95	APN	17	18,660,473 (GRCm39)	missense	probably benign	0.06
IGL02113:Vmn2r95	APN	17	18,660,169 (GRCm39)	missense	possibly damaging	0.47
IGL02154:Vmn2r95	APN	17	18,672,248 (GRCm39)	missense	probably benign	0.16
IGL02190:Vmn2r95	APN	17	18,672,038 (GRCm39)	missense	probably benign	0.00
IGL02412:Vmn2r95	APN	17	18,660,218 (GRCm39)	missense	probably damaging	1.00
IGL02550:Vmn2r95	APN	17	18,671,994 (GRCm39)	missense	probably damaging	1.00
IGL02679:Vmn2r95	APN	17	18,664,116 (GRCm39)	missense	probably damaging	1.00
IGL02691:Vmn2r95	APN	17	18,672,120 (GRCm39)	missense	probably benign	0.07
IGL02990:Vmn2r95	APN	17	18,672,298 (GRCm39)	nonsense	probably null
IGL03032:Vmn2r95	APN	17	18,672,575 (GRCm39)	missense	probably benign	0.00
R0416:Vmn2r95	UTSW	17	18,661,664 (GRCm39)	missense	probably damaging	1.00
R0448:Vmn2r95	UTSW	17	18,672,005 (GRCm39)	missense	possibly damaging	0.92
R0514:Vmn2r95	UTSW	17	18,671,844 (GRCm39)	missense	probably benign
R0519:Vmn2r95	UTSW	17	18,659,765 (GRCm39)	missense	probably damaging	1.00
R0539:Vmn2r95	UTSW	17	18,672,362 (GRCm39)	missense	probably damaging	1.00
R1501:Vmn2r95	UTSW	17	18,660,118 (GRCm39)	missense	probably damaging	0.99
R1598:Vmn2r95	UTSW	17	18,672,575 (GRCm39)	missense	probably benign	0.03
R1613:Vmn2r95	UTSW	17	18,660,901 (GRCm39)	splice site	probably benign
R1861:Vmn2r95	UTSW	17	18,672,530 (GRCm39)	missense	probably damaging	1.00
R1921:Vmn2r95	UTSW	17	18,644,575 (GRCm39)	missense	probably benign	0.11
R1986:Vmn2r95	UTSW	17	18,671,805 (GRCm39)	missense	probably benign
R2031:Vmn2r95	UTSW	17	18,659,717 (GRCm39)	missense	possibly damaging	0.94
R2040:Vmn2r95	UTSW	17	18,661,561 (GRCm39)	missense	probably damaging	1.00
R3608:Vmn2r95	UTSW	17	18,660,235 (GRCm39)	missense	possibly damaging	0.47
R3727:Vmn2r95	UTSW	17	18,661,744 (GRCm39)	nonsense	probably null
R3953:Vmn2r95	UTSW	17	18,660,358 (GRCm39)	missense	possibly damaging	0.79
R3955:Vmn2r95	UTSW	17	18,660,358 (GRCm39)	missense	possibly damaging	0.79
R3957:Vmn2r95	UTSW	17	18,660,358 (GRCm39)	missense	possibly damaging	0.79
R4474:Vmn2r95	UTSW	17	18,672,507 (GRCm39)	missense	probably damaging	1.00
R4672:Vmn2r95	UTSW	17	18,672,413 (GRCm39)	missense	probably damaging	1.00
R4850:Vmn2r95	UTSW	17	18,671,915 (GRCm39)	missense	probably damaging	1.00
R5054:Vmn2r95	UTSW	17	18,671,708 (GRCm39)	missense	possibly damaging	0.63
R5980:Vmn2r95	UTSW	17	18,661,624 (GRCm39)	missense	probably benign
R6183:Vmn2r95	UTSW	17	18,664,192 (GRCm39)	missense	probably damaging	0.99
R6276:Vmn2r95	UTSW	17	18,671,732 (GRCm39)	missense	possibly damaging	0.96
R6651:Vmn2r95	UTSW	17	18,660,622 (GRCm39)	missense	probably damaging	1.00
R6682:Vmn2r95	UTSW	17	18,660,489 (GRCm39)	missense	probably damaging	1.00
R6797:Vmn2r95	UTSW	17	18,672,551 (GRCm39)	utr 3 prime	probably benign
R6799:Vmn2r95	UTSW	17	18,659,555 (GRCm39)	missense	probably damaging	1.00
R6849:Vmn2r95	UTSW	17	18,664,182 (GRCm39)	missense	probably damaging	1.00
R6849:Vmn2r95	UTSW	17	18,664,181 (GRCm39)	missense	probably damaging	1.00
R6982:Vmn2r95	UTSW	17	18,672,323 (GRCm39)	missense	probably damaging	1.00
R7203:Vmn2r95	UTSW	17	18,661,577 (GRCm39)	missense	probably benign	0.01
R7226:Vmn2r95	UTSW	17	18,672,245 (GRCm39)	missense	possibly damaging	0.90
R7240:Vmn2r95	UTSW	17	18,672,225 (GRCm39)	missense	probably benign	0.15
R7383:Vmn2r95	UTSW	17	18,660,734 (GRCm39)	missense	probably benign	0.06
R7614:Vmn2r95	UTSW	17	18,660,352 (GRCm39)	missense	probably benign
R7755:Vmn2r95	UTSW	17	18,644,367 (GRCm39)	start codon destroyed	probably null	0.99
R7942:Vmn2r95	UTSW	17	18,660,529 (GRCm39)	missense	possibly damaging	0.74
R8355:Vmn2r95	UTSW	17	18,660,352 (GRCm39)	missense	probably benign
R8455:Vmn2r95	UTSW	17	18,660,352 (GRCm39)	missense	probably benign
R8478:Vmn2r95	UTSW	17	18,672,544 (GRCm39)	missense	probably damaging	1.00
R8547:Vmn2r95	UTSW	17	18,664,161 (GRCm39)	missense	probably damaging	1.00
R8752:Vmn2r95	UTSW	17	18,661,738 (GRCm39)	missense	probably damaging	0.98
R8788:Vmn2r95	UTSW	17	18,671,790 (GRCm39)	missense	probably benign	0.09
R8852:Vmn2r95	UTSW	17	18,664,113 (GRCm39)	missense	possibly damaging	0.95
R9098:Vmn2r95	UTSW	17	18,660,167 (GRCm39)	missense	possibly damaging	0.88
R9202:Vmn2r95	UTSW	17	18,644,394 (GRCm39)	missense	probably benign	0.00
R9244:Vmn2r95	UTSW	17	18,672,189 (GRCm39)	missense	possibly damaging	0.91
R9546:Vmn2r95	UTSW	17	18,661,721 (GRCm39)	missense	probably benign	0.01
R9665:Vmn2r95	UTSW	17	18,660,607 (GRCm39)	missense	probably damaging	0.99
Z1088:Vmn2r95	UTSW	17	18,660,663 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- GACCATGGTCAGTATCCTTCTC -3'
(R):5'- GAGTCTAACATGAAATAGTCAGCTCTG -3'

Sequencing Primer
(F):5'- AGTATCCTTCTCTCTACCAGATGG -3'
(R):5'- GTCAGCTCTGTCAATAACATGATG -3'

Posted On

2016-07-06