Mutagenetix > Incidental Mutation

Incidental Mutation 'R0456:Fras1'

40008

Institutional Source

Beutler Lab

Gene Symbol

Fras1

Ensembl Gene

ENSMUSG00000034687

Gene Name

Fraser extracellular matrix complex subunit 1

Synonyms

bl, E130113P14Rik

MMRRC Submission

038656-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R0456 (G1)

Quality Score

200

Status

Not validated

Chromosome

Chromosomal Location

96373955-96784728 bp(+) (GRCm38)

Type of Mutation

splice site

DNA Base Change (assembly)

T to G at 96714343 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000043250 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000036019]

AlphaFold

no structure available at present

Predicted Effect

probably null
Transcript: ENSMUST00000036019

SMART Domains

Protein: ENSMUSP00000043250
Gene: ENSMUSG00000034687

Domain	Start	End	E-Value	Type
signal peptide	1	25	N/A	INTRINSIC
VWC	27	86	9.23e-9	SMART
VWC	94	151	9.37e-10	SMART
VWC	158	215	8.61e-9	SMART
VWC	220	277	7.1e-10	SMART
VWC	284	341	7.8e-7	SMART
VWC	365	415	1.93e-1	SMART
FU	408	459	3.33e-1	SMART
FU	461	504	9.12e-8	SMART
EGF_like	466	495	6.67e1	SMART
FU	506	552	6.01e-8	SMART
FU	554	598	2.21e-6	SMART
FU	601	646	1.28e-11	SMART
FU	648	704	4.19e-7	SMART
FU	707	752	9.12e-8	SMART
FU	754	799	1.11e-6	SMART
EGF_like	759	790	7.23e1	SMART
FU	802	851	5.44e-6	SMART
EGF_like	807	842	4.55e1	SMART
FU	853	899	7.4e-8	SMART
FU	902	947	4.78e-2	SMART
FU	951	996	4.52e-12	SMART
EGF_like	956	987	2.75e1	SMART
FU	998	1041	1.38e-7	SMART
FU	1045	1088	9.7e-3	SMART
EGF_like	1057	1096	3.16e1	SMART
Pfam:Cadherin_3	1098	1198	5.2e-12	PFAM
Pfam:Cadherin_3	1167	1309	6.5e-27	PFAM
Pfam:Cadherin_3	1278	1442	7e-24	PFAM
Pfam:Cadherin_3	1411	1560	1.3e-23	PFAM
Pfam:Cadherin_3	1561	1693	6.4e-15	PFAM
Pfam:Cadherin_3	1695	1814	1.1e-10	PFAM
Pfam:Cadherin_3	1780	1940	1.6e-18	PFAM
Pfam:Cadherin_3	1906	2061	2.8e-22	PFAM
Pfam:Cadherin_3	2063	2181	3.3e-18	PFAM
Pfam:Cadherin_3	2172	2295	1.4e-26	PFAM
Pfam:Cadherin_3	2296	2408	2.3e-31	PFAM
Pfam:Cadherin_3	2413	2540	7.9e-23	PFAM
Calx_beta	2544	2648	1.23e-10	SMART
Calx_beta	2661	2772	3.3e-11	SMART
Calx_beta	2787	2892	1.21e-9	SMART
Calx_beta	2907	3009	4.45e-3	SMART
Calx_beta	3027	3131	1.5e-14	SMART
Blast:Calx_beta	3162	3187	1e-5	BLAST
transmembrane domain	3902	3924	N/A	INTRINSIC
low complexity region	3927	3935	N/A	INTRINSIC

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 97.1%
20x: 95.1%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes an extracellular matrix protein that appears to function in the regulation of epidermal-basement membrane adhesion and organogenesis during development. Mutations in this gene cause Fraser syndrome, a multisystem malformation that can include craniofacial, urogenital and respiratory system abnormalities. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
PHENOTYPE: Mice homozygous for mutations at this locus display a significant amount of embryonic lethality due to hemorrhaging of embryonic blisters. Survival is variable on genetic backgrounds. Kidney development is severely affected and syndactyly is common. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ankrd29	G	A	18: 12,295,979	P11L	probably damaging	Het
Antxr1	A	C	6: 87,217,275	V347G	probably damaging	Het
Atp13a5	T	C	16: 29,232,740	N1127D	probably benign	Het
Bivm	T	A	1: 44,126,809	W140R	probably damaging	Het
Camta1	C	A	4: 151,075,140	R1614L	probably damaging	Het
Ceacam5	T	C	7: 17,760,851	V928A	possibly damaging	Het
Chia1	T	C	3: 106,128,479	Y152H	probably damaging	Het
Chsy3	A	T	18: 59,176,478	I268F	probably damaging	Het
CK137956	A	T	4: 127,945,307	N439K	probably damaging	Het
Csf3r	C	A	4: 126,035,861	N392K	probably damaging	Het
Cyp1b1	T	C	17: 79,710,275	I484V	probably benign	Het
Dsc1	T	C	18: 20,099,112	K280E	probably damaging	Het
Eefsec	A	G	6: 88,297,888	Y365H	probably benign	Het
Epb41l4b	C	T	4: 57,142,843		probably null	Het
Erap1	G	A	13: 74,664,220	V385I	probably benign	Het
Fat1	A	T	8: 45,029,534	I3077F	probably damaging	Het
Gsg1l	A	G	7: 125,923,510	M182T	possibly damaging	Het
Gzmg	A	G	14: 56,158,322	V60A	probably damaging	Het
Hapln4	A	T	8: 70,084,995	Y113F	probably benign	Het
Hist1h4f	T	C	13: 23,551,387	D86G	probably damaging	Het
Ikzf4	T	A	10: 128,635,808	T274S	probably damaging	Het
Kansl1l	T	A	1: 66,735,726	H302L	probably damaging	Het
Klhl41	T	C	2: 69,670,549	V118A	probably damaging	Het
Kpna1	T	C	16: 36,002,900	S41P	possibly damaging	Het
Krt23	A	G	11: 99,486,778	V134A	probably benign	Het
Lamb1	G	A	12: 31,304,730	C992Y	probably damaging	Het
Lrif1	C	T	3: 106,731,778	P35S	probably benign	Het
Lrrc4	A	G	6: 28,831,104	S171P	probably damaging	Het
Lvrn	G	A	18: 46,864,816		probably null	Het
Matr3	T	A	18: 35,572,864	F281I	probably damaging	Het
Nxn	G	A	11: 76,263,137	Q291*	probably null	Het
Olfr1467	A	G	19: 13,364,738	T37A	probably damaging	Het
Pdp2	G	T	8: 104,593,789	R90L	probably damaging	Het
Ppp1r3c	C	A	19: 36,733,891	E160*	probably null	Het
Ppp2r5c	T	G	12: 110,522,579	S118R	probably damaging	Het
Ptpn23	G	T	9: 110,389,793		probably null	Het
Ptpro	G	T	6: 137,414,230	V783L	probably benign	Het
Rab40c	A	G	17: 25,884,657	V144A	possibly damaging	Het
Rasal2	T	C	1: 157,149,843	N1087S	probably damaging	Het
Rfc3	T	A	5: 151,647,523	S103C	possibly damaging	Het
Rgl2	A	G	17: 33,936,849		probably null	Het
Rif1	GCCACCA	GCCA	2: 52,110,324		probably benign	Het
Rit2	A	T	18: 30,975,451	F160L	probably benign	Het
Rnh1	A	G	7: 141,162,548	S366P	possibly damaging	Het
Sdk2	T	C	11: 113,791,466	Y2029C	possibly damaging	Het
Smpd3	T	A	8: 106,259,656	I505F	probably benign	Het
Sorcs3	A	T	19: 48,654,044	S379C	possibly damaging	Het
Sult1e1	A	G	5: 87,578,634	L207P	possibly damaging	Het
Sycp2	C	G	2: 178,381,855	S456T	probably benign	Het
Syne1	T	C	10: 5,342,252	T1339A	probably benign	Het
Tas2r117	A	G	6: 132,803,391	N164S	probably benign	Het
Tigd3	A	G	19: 5,892,793	L103P	probably damaging	Het
Tldc1	A	T	8: 119,768,423	F199I	probably damaging	Het
Tmem132b	T	C	5: 125,787,724	S965P	probably damaging	Het
Tmem82	T	C	4: 141,617,390	T81A	probably benign	Het
Tmem8b	A	G	4: 43,685,618	T156A	probably benign	Het
Tnfrsf21	A	G	17: 43,038,091	E198G	probably benign	Het
Tnpo2	A	G	8: 85,054,416	N767S	probably damaging	Het
Trf	T	C	9: 103,226,903	Y87C	probably damaging	Het
Tst	A	G	15: 78,405,580	V85A	probably damaging	Het
Usp37	A	T	1: 74,468,348	N503K	probably damaging	Het
Utp20	C	T	10: 88,754,573	M2346I	possibly damaging	Het
Vax2	A	G	6: 83,711,406	D37G	probably benign	Het
Vmn1r77	T	A	7: 12,041,738	L79*	probably null	Het
Zbtb3	A	T	19: 8,803,200	D59V	probably damaging	Het
Zdhhc13	T	C	7: 48,808,854	F182S	probably benign	Het
Zfp426	A	G	9: 20,470,297	F465L	probably damaging	Het
Zfp526	A	G	7: 25,226,212	E632G	probably damaging	Het

Other mutations in Fras1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00332:Fras1	APN	5	96739358	missense	possibly damaging	0.55
IGL00507:Fras1	APN	5	96778189	missense	probably damaging	1.00
IGL00672:Fras1	APN	5	96759450	splice site	probably benign
IGL00772:Fras1	APN	5	96636112	missense	probably benign	0.42
IGL00844:Fras1	APN	5	96534853	splice site	probably benign
IGL00913:Fras1	APN	5	96695076	missense	probably damaging	0.99
IGL00959:Fras1	APN	5	96781281	missense	probably damaging	0.96
IGL00966:Fras1	APN	5	96555221	missense	probably benign	0.00
IGL01296:Fras1	APN	5	96673698	missense	probably null	0.58
IGL01307:Fras1	APN	5	96781692	missense	probably benign
IGL01481:Fras1	APN	5	96657241	missense	probably damaging	1.00
IGL01525:Fras1	APN	5	96739336	missense	probably damaging	0.99
IGL01599:Fras1	APN	5	96709891	missense	possibly damaging	0.94
IGL01646:Fras1	APN	5	96758148	missense	probably benign	0.29
IGL01795:Fras1	APN	5	96778045	missense	probably damaging	1.00
IGL01867:Fras1	APN	5	96588131	missense	probably benign
IGL01869:Fras1	APN	5	96708783	splice site	probably benign
IGL01923:Fras1	APN	5	96735280	missense	probably damaging	1.00
IGL01982:Fras1	APN	5	96739248	missense	possibly damaging	0.46
IGL02109:Fras1	APN	5	96700523	missense	probably benign
IGL02132:Fras1	APN	5	96781637	nonsense	probably null
IGL02171:Fras1	APN	5	96735181	missense	probably benign	0.15
IGL02213:Fras1	APN	5	96645871	nonsense	probably null
IGL02277:Fras1	APN	5	96588118	missense	probably benign	0.00
IGL02507:Fras1	APN	5	96657408	missense	possibly damaging	0.95
IGL02589:Fras1	APN	5	96769513	missense	probably damaging	1.00
IGL02671:Fras1	APN	5	96728616	missense	possibly damaging	0.91
IGL02677:Fras1	APN	5	96545024	missense	probably damaging	1.00
IGL02691:Fras1	APN	5	96744705	missense	possibly damaging	0.68
IGL02741:Fras1	APN	5	96691371	missense	probably benign	0.35
IGL02836:Fras1	APN	5	96534866	missense	possibly damaging	0.67
IGL02850:Fras1	APN	5	96778175	missense	probably damaging	1.00
IGL02998:Fras1	APN	5	96702181	missense	possibly damaging	0.82
IGL03040:Fras1	APN	5	96710101	missense	probably benign
IGL03078:Fras1	APN	5	96636135	missense	probably damaging	1.00
IGL03096:Fras1	APN	5	96764901	missense	probably damaging	1.00
IGL03102:Fras1	APN	5	96726535	missense	probably benign	0.11
IGL03183:Fras1	APN	5	96733781	splice site	probably benign
IGL03189:Fras1	APN	5	96743071	missense	probably benign	0.00
IGL03193:Fras1	APN	5	96778106	missense	probably damaging	0.99
IGL03292:Fras1	APN	5	96707491	missense	probably damaging	1.00
IGL03328:Fras1	APN	5	96781760	missense	probably damaging	0.96
IGL03335:Fras1	APN	5	96733944	splice site	probably benign
IGL03394:Fras1	APN	5	96667477	missense	probably damaging	0.98
IGL03404:Fras1	APN	5	96728581	missense	probably damaging	0.99
baby_ruth	UTSW	5	96708758	missense	probably benign	0.01
BB002:Fras1	UTSW	5	96781584	missense	probably damaging	0.96
BB012:Fras1	UTSW	5	96781584	missense	probably damaging	0.96
G1patch:Fras1	UTSW	5	96781340	missense	possibly damaging	0.91
I0000:Fras1	UTSW	5	96740829	missense	probably damaging	0.99
PIT4581001:Fras1	UTSW	5	96555301	missense	probably benign	0.01
R0028:Fras1	UTSW	5	96677316	missense	probably benign	0.07
R0049:Fras1	UTSW	5	96776622	missense	probably benign	0.07
R0049:Fras1	UTSW	5	96776622	missense	probably benign	0.07
R0099:Fras1	UTSW	5	96614917	critical splice donor site	probably null
R0109:Fras1	UTSW	5	96710077	missense	probably benign	0.01
R0158:Fras1	UTSW	5	96776634	missense	possibly damaging	0.83
R0268:Fras1	UTSW	5	96737009	missense	probably damaging	0.99
R0305:Fras1	UTSW	5	96596888	missense	probably benign
R0352:Fras1	UTSW	5	96726540	missense	probably damaging	0.97
R0359:Fras1	UTSW	5	96762590	missense	probably damaging	0.98
R0371:Fras1	UTSW	5	96555331	missense	possibly damaging	0.90
R0379:Fras1	UTSW	5	96755509	nonsense	probably null
R0395:Fras1	UTSW	5	96769653	missense	possibly damaging	0.50
R0417:Fras1	UTSW	5	96691372	missense	probably benign	0.18
R0454:Fras1	UTSW	5	96762665	missense	probably damaging	0.96
R0456:Fras1	UTSW	5	96554788	missense	probably damaging	1.00
R0464:Fras1	UTSW	5	96636803	missense	probably damaging	0.98
R0613:Fras1	UTSW	5	96700488	splice site	probably benign
R0652:Fras1	UTSW	5	96781340	missense	possibly damaging	0.91
R0675:Fras1	UTSW	5	96667387	splice site	probably benign
R0765:Fras1	UTSW	5	96552796	missense	probably benign	0.00
R0783:Fras1	UTSW	5	96768430	missense	probably damaging	1.00
R0811:Fras1	UTSW	5	96752998	missense	probably benign	0.35
R0812:Fras1	UTSW	5	96752998	missense	probably benign	0.35
R0943:Fras1	UTSW	5	96726543	missense	probably benign	0.00
R1037:Fras1	UTSW	5	96714463	missense	probably damaging	0.97
R1104:Fras1	UTSW	5	96708671	missense	probably benign	0.00
R1108:Fras1	UTSW	5	96642629	missense	probably damaging	0.99
R1332:Fras1	UTSW	5	96707308	missense	probably benign	0.00
R1336:Fras1	UTSW	5	96707308	missense	probably benign	0.00
R1458:Fras1	UTSW	5	96600733	missense	probably benign	0.00
R1495:Fras1	UTSW	5	96528586	missense	possibly damaging	0.49
R1499:Fras1	UTSW	5	96743187	missense	probably benign	0.31
R1528:Fras1	UTSW	5	96636819	missense	probably damaging	0.99
R1532:Fras1	UTSW	5	96713996	missense	probably damaging	1.00
R1556:Fras1	UTSW	5	96743062	missense	possibly damaging	0.88
R1625:Fras1	UTSW	5	96709978	missense	possibly damaging	0.94
R1625:Fras1	UTSW	5	96713990	missense	probably damaging	1.00
R1645:Fras1	UTSW	5	96700586	missense	possibly damaging	0.90
R1647:Fras1	UTSW	5	96726613	critical splice donor site	probably null
R1648:Fras1	UTSW	5	96726613	critical splice donor site	probably null
R1661:Fras1	UTSW	5	96598909	missense	probably damaging	1.00
R1665:Fras1	UTSW	5	96598909	missense	probably damaging	1.00
R1682:Fras1	UTSW	5	96645873	missense	probably benign	0.00
R1701:Fras1	UTSW	5	96600784	missense	probably benign	0.00
R1716:Fras1	UTSW	5	96552725	missense	probably benign	0.10
R1718:Fras1	UTSW	5	96554889	splice site	probably null
R1800:Fras1	UTSW	5	96709882	missense	probably benign
R1806:Fras1	UTSW	5	96713970	splice site	probably benign
R1806:Fras1	UTSW	5	96764976	missense	possibly damaging	0.88
R1822:Fras1	UTSW	5	96770688	missense	probably damaging	1.00
R1823:Fras1	UTSW	5	96770688	missense	probably damaging	1.00
R1824:Fras1	UTSW	5	96770688	missense	probably damaging	1.00
R1847:Fras1	UTSW	5	96749423	splice site	probably null
R1929:Fras1	UTSW	5	96667437	missense	probably benign	0.24
R1951:Fras1	UTSW	5	96712383	missense	probably benign	0.38
R2093:Fras1	UTSW	5	96781203	missense	probably damaging	1.00
R2283:Fras1	UTSW	5	96654305	missense	probably benign	0.10
R2884:Fras1	UTSW	5	96700268	missense	probably benign	0.07
R2913:Fras1	UTSW	5	96733915	missense	probably benign
R2914:Fras1	UTSW	5	96733915	missense	probably benign
R3054:Fras1	UTSW	5	96764943	missense	probably damaging	0.99
R3117:Fras1	UTSW	5	96771712	missense	probably damaging	1.00
R3118:Fras1	UTSW	5	96771712	missense	probably damaging	1.00
R3691:Fras1	UTSW	5	96781512	missense	probably benign	0.02
R3714:Fras1	UTSW	5	96645970	critical splice donor site	probably null
R3715:Fras1	UTSW	5	96645970	critical splice donor site	probably null
R3801:Fras1	UTSW	5	96733932	missense	probably benign	0.26
R3961:Fras1	UTSW	5	96677385	critical splice donor site	probably null
R4065:Fras1	UTSW	5	96770683	missense	possibly damaging	0.64
R4066:Fras1	UTSW	5	96770683	missense	possibly damaging	0.64
R4076:Fras1	UTSW	5	96743158	missense	probably damaging	1.00
R4124:Fras1	UTSW	5	96770653	missense	probably benign	0.05
R4127:Fras1	UTSW	5	96770653	missense	probably benign	0.05
R4153:Fras1	UTSW	5	96776735	missense	probably benign	0.17
R4233:Fras1	UTSW	5	96714376	missense	possibly damaging	0.91
R4273:Fras1	UTSW	5	96614904	missense	probably benign	0.00
R4355:Fras1	UTSW	5	96700242	missense	probably benign
R4401:Fras1	UTSW	5	96642620	missense	probably damaging	0.97
R4402:Fras1	UTSW	5	96642620	missense	probably damaging	0.97
R4403:Fras1	UTSW	5	96642620	missense	probably damaging	0.97
R4505:Fras1	UTSW	5	96781348	missense	probably damaging	1.00
R4548:Fras1	UTSW	5	96709895	missense	probably benign	0.00
R4559:Fras1	UTSW	5	96781289	missense	probably damaging	1.00
R4629:Fras1	UTSW	5	96776734	missense	probably benign	0.00
R4637:Fras1	UTSW	5	96778088	missense	probably damaging	1.00
R4678:Fras1	UTSW	5	96700568	missense	probably benign	0.13
R4707:Fras1	UTSW	5	96735238	missense	probably damaging	0.96
R4735:Fras1	UTSW	5	96588163	missense	probably benign	0.00
R4756:Fras1	UTSW	5	96781659	missense	probably benign	0.00
R4762:Fras1	UTSW	5	96731618	missense	probably benign
R4820:Fras1	UTSW	5	96728653	missense	probably benign	0.00
R4847:Fras1	UTSW	5	96544992	missense	possibly damaging	0.94
R4857:Fras1	UTSW	5	96778159	missense	probably benign	0.00
R4909:Fras1	UTSW	5	96708758	missense	probably benign	0.01
R4931:Fras1	UTSW	5	96636840	missense	probably benign	0.02
R4938:Fras1	UTSW	5	96776724	missense	probably damaging	0.99
R4952:Fras1	UTSW	5	96647498	missense	probably benign	0.01
R4965:Fras1	UTSW	5	96726580	missense	possibly damaging	0.95
R4989:Fras1	UTSW	5	96650682	missense	possibly damaging	0.75
R5151:Fras1	UTSW	5	96645110	missense	probably damaging	1.00
R5168:Fras1	UTSW	5	96708757	missense	probably benign	0.00
R5182:Fras1	UTSW	5	96636173	nonsense	probably null
R5214:Fras1	UTSW	5	96769593	missense	probably damaging	1.00
R5220:Fras1	UTSW	5	96768363	missense	probably damaging	1.00
R5235:Fras1	UTSW	5	96600750	missense	probably benign	0.02
R5242:Fras1	UTSW	5	96657250	missense	probably benign	0.11
R5253:Fras1	UTSW	5	96741025	missense	probably damaging	0.99
R5260:Fras1	UTSW	5	96735187	missense	possibly damaging	0.79
R5301:Fras1	UTSW	5	96657266	missense	possibly damaging	0.88
R5411:Fras1	UTSW	5	96645160	missense	probably benign	0.00
R5467:Fras1	UTSW	5	96780053	missense	probably benign	0.04
R5543:Fras1	UTSW	5	96528535	missense	probably benign	0.01
R5555:Fras1	UTSW	5	96677377	missense	probably benign	0.34
R5602:Fras1	UTSW	5	96737021	missense	probably damaging	1.00
R5664:Fras1	UTSW	5	96728535	missense	possibly damaging	0.91
R5695:Fras1	UTSW	5	96781344	missense	probably damaging	1.00
R5717:Fras1	UTSW	5	96781737	missense	possibly damaging	0.93
R5742:Fras1	UTSW	5	96768381	missense	possibly damaging	0.50
R5759:Fras1	UTSW	5	96709916	missense	probably benign	0.02
R5766:Fras1	UTSW	5	96731689	missense	possibly damaging	0.91
R5890:Fras1	UTSW	5	96645948	missense	probably benign
R6052:Fras1	UTSW	5	96764866	missense	probably damaging	1.00
R6058:Fras1	UTSW	5	96709985	missense	probably benign
R6256:Fras1	UTSW	5	96733843	missense	possibly damaging	0.84
R6306:Fras1	UTSW	5	96764946	missense	probably damaging	1.00
R6494:Fras1	UTSW	5	96759564	missense	possibly damaging	0.59
R6638:Fras1	UTSW	5	96758094	missense	possibly damaging	0.94
R6647:Fras1	UTSW	5	96735202	missense	probably damaging	1.00
R6725:Fras1	UTSW	5	96781340	missense	possibly damaging	0.91
R6769:Fras1	UTSW	5	96598941	missense	possibly damaging	0.60
R6771:Fras1	UTSW	5	96598941	missense	possibly damaging	0.60
R6837:Fras1	UTSW	5	96726973	missense	probably damaging	0.99
R6841:Fras1	UTSW	5	96728551	missense	probably damaging	0.99
R6863:Fras1	UTSW	5	96543306	missense	probably benign	0.19
R6868:Fras1	UTSW	5	96682378	missense	probably benign	0.38
R6936:Fras1	UTSW	5	96768352	missense	possibly damaging	0.92
R6997:Fras1	UTSW	5	96614873	nonsense	probably null
R7023:Fras1	UTSW	5	96710084	missense	probably benign	0.00
R7091:Fras1	UTSW	5	96708676	missense	probably benign
R7102:Fras1	UTSW	5	96571041	missense	probably benign
R7120:Fras1	UTSW	5	96752960	nonsense	probably null
R7124:Fras1	UTSW	5	96714401	missense	probably damaging	1.00
R7129:Fras1	UTSW	5	96781284	missense	probably benign	0.00
R7173:Fras1	UTSW	5	96778078	missense	probably damaging	1.00
R7174:Fras1	UTSW	5	96755577	critical splice donor site	probably null
R7185:Fras1	UTSW	5	96636776	missense	probably damaging	1.00
R7191:Fras1	UTSW	5	96614912	missense	probably benign	0.05
R7216:Fras1	UTSW	5	96739314	missense	probably damaging	1.00
R7222:Fras1	UTSW	5	96636186	missense	probably damaging	1.00
R7222:Fras1	UTSW	5	96636809	missense	probably benign	0.00
R7320:Fras1	UTSW	5	96709886	missense	probably benign	0.03
R7335:Fras1	UTSW	5	96736970	missense	possibly damaging	0.82
R7378:Fras1	UTSW	5	96596785	missense	probably damaging	0.98
R7394:Fras1	UTSW	5	96712450	nonsense	probably null
R7412:Fras1	UTSW	5	96614889	missense	probably benign	0.06
R7422:Fras1	UTSW	5	96673599	missense	probably benign	0.21
R7552:Fras1	UTSW	5	96768438	missense	probably damaging	1.00
R7559:Fras1	UTSW	5	96740854	missense	possibly damaging	0.82
R7575:Fras1	UTSW	5	96543314	missense	probably benign	0.02
R7578:Fras1	UTSW	5	96684437	missense	probably damaging	1.00
R7600:Fras1	UTSW	5	96684436	missense	probably damaging	1.00
R7669:Fras1	UTSW	5	96692624	missense	probably benign	0.01
R7710:Fras1	UTSW	5	96645103	nonsense	probably null
R7722:Fras1	UTSW	5	96769554	missense	probably damaging	1.00
R7726:Fras1	UTSW	5	96712451	missense	probably benign	0.41
R7745:Fras1	UTSW	5	96726895	missense	probably benign	0.11
R7777:Fras1	UTSW	5	96752904	missense	probably damaging	1.00
R7923:Fras1	UTSW	5	96739318	missense	probably damaging	1.00
R7925:Fras1	UTSW	5	96781584	missense	probably damaging	0.96
R8000:Fras1	UTSW	5	96762677	missense	probably damaging	0.96
R8056:Fras1	UTSW	5	96744774	missense	probably damaging	1.00
R8058:Fras1	UTSW	5	96694919	missense	probably benign
R8117:Fras1	UTSW	5	96707386	missense	probably damaging	1.00
R8157:Fras1	UTSW	5	96554855	missense	probably benign	0.00
R8312:Fras1	UTSW	5	96588191	missense	probably benign
R8315:Fras1	UTSW	5	96743182	missense	probably damaging	0.97
R8412:Fras1	UTSW	5	96596852	missense	probably benign	0.00
R8546:Fras1	UTSW	5	96709966	missense	probably benign	0.00
R8705:Fras1	UTSW	5	96691401	missense	probably benign	0.01
R8790:Fras1	UTSW	5	96755377	missense	probably damaging	1.00
R8848:Fras1	UTSW	5	96781348	missense	probably damaging	1.00
R8855:Fras1	UTSW	5	96769606	missense
R8871:Fras1	UTSW	5	96707398	missense	probably benign	0.34
R8894:Fras1	UTSW	5	96759543	missense	probably damaging	1.00
R8904:Fras1	UTSW	5	96781279	missense	probably benign	0.16
R8910:Fras1	UTSW	5	96567996	missense	probably benign	0.01
R8916:Fras1	UTSW	5	96752915	missense	probably damaging	1.00
R8929:Fras1	UTSW	5	96769507	missense	probably damaging	0.98
R8995:Fras1	UTSW	5	96712556	missense	possibly damaging	0.83
R9016:Fras1	UTSW	5	96636064	missense	probably damaging	0.96
R9021:Fras1	UTSW	5	96740750	missense	probably damaging	1.00
R9038:Fras1	UTSW	5	96726883	missense	probably benign	0.25
R9204:Fras1	UTSW	5	96735163	missense	probably damaging	1.00
R9222:Fras1	UTSW	5	96657228	missense	probably benign	0.15
R9231:Fras1	UTSW	5	96545045	missense	probably damaging	1.00
R9238:Fras1	UTSW	5	96684361	missense	possibly damaging	0.90
R9257:Fras1	UTSW	5	96762500	missense	probably damaging	1.00
R9302:Fras1	UTSW	5	96534892	missense	probably damaging	1.00
R9329:Fras1	UTSW	5	96736954	missense	probably damaging	1.00
R9361:Fras1	UTSW	5	96776698	missense	probably damaging	1.00
R9430:Fras1	UTSW	5	96781392	missense	probably benign	0.00
R9431:Fras1	UTSW	5	96753014	missense	possibly damaging	0.71
R9474:Fras1	UTSW	5	96739265	missense	probably benign	0.00
R9475:Fras1	UTSW	5	96780070	missense	probably damaging	0.96
R9497:Fras1	UTSW	5	96737036	missense	probably damaging	1.00
R9597:Fras1	UTSW	5	96740692	missense	probably damaging	1.00
R9650:Fras1	UTSW	5	96762528	missense	probably damaging	1.00
R9694:Fras1	UTSW	5	96781686	missense	probably benign	0.03
R9779:Fras1	UTSW	5	96569494	missense	probably damaging	0.99
Z1088:Fras1	UTSW	5	96743211	missense	probably damaging	0.97
Z1088:Fras1	UTSW	5	96758142	missense	probably benign
Z1176:Fras1	UTSW	5	96758142	missense	probably benign
Z1177:Fras1	UTSW	5	96691457	missense	probably damaging	1.00
Z1177:Fras1	UTSW	5	96700251	missense	possibly damaging	0.71
Z1177:Fras1	UTSW	5	96740811	missense	probably benign	0.18
Z1177:Fras1	UTSW	5	96740983	missense	possibly damaging	0.95
Z1177:Fras1	UTSW	5	96758142	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- TGGCTGACCAGTCATTTTCCATCG -3'
(R):5'- TGTAACAGTCCCCAGCACTTGC -3'

Sequencing Primer
(F):5'- GGGGTCTTCCATTCCCACAG -3'
(R):5'- CTTGCTTAGGGGAAGTGAACC -3'

Posted On

2013-05-23