Mutagenetix > Incidental Mutation

Incidental Mutation 'R5238:Zfp600'

400482

Institutional Source

Beutler Lab

Gene Symbol

Zfp600

Ensembl Gene

ENSMUSG00000066007

Gene Name

zinc finger protein 600

Synonyms

Gm13164

MMRRC Submission

042809-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.397) question?

Stock #

R5238 (G1)

Quality Score

157

Status

Not validated

Chromosome

Chromosomal Location

146093397-146135326 bp(+) (GRCm39)

Type of Mutation

splice site (2 bp from exon)

DNA Base Change (assembly)

T to C at 146131741 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000117892 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000092750] [ENSMUST00000131932] [ENSMUST00000168483]

AlphaFold

A2A7V0

Predicted Effect

silent
Transcript: ENSMUST00000092750

SMART Domains

Protein: ENSMUSP00000090425
Gene: ENSMUSG00000066007

Domain	Start	End	E-Value	Type
KRAB	28	90	1.29e-17	SMART
ZnF_C2H2	251	273	7.9e-4	SMART
ZnF_C2H2	279	301	7.9e-4	SMART
ZnF_C2H2	307	329	3.49e-5	SMART
ZnF_C2H2	335	357	1.95e-3	SMART
ZnF_C2H2	363	385	7.9e-4	SMART
ZnF_C2H2	391	413	7.9e-4	SMART
ZnF_C2H2	419	441	3.49e-5	SMART
ZnF_C2H2	447	469	3.89e-3	SMART
ZnF_C2H2	475	497	2.75e-3	SMART
ZnF_C2H2	503	525	3.04e-5	SMART
ZnF_C2H2	531	553	8.34e-3	SMART
ZnF_C2H2	559	581	5.67e-5	SMART
ZnF_C2H2	587	609	1.98e-4	SMART
ZnF_C2H2	615	637	5.29e-5	SMART
ZnF_C2H2	643	665	1.58e-3	SMART
ZnF_C2H2	671	693	1.28e-3	SMART
ZnF_C2H2	699	721	1.6e-4	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000131932

SMART Domains

Protein: ENSMUSP00000117892
Gene: ENSMUSG00000066007

Domain	Start	End	E-Value	Type
KRAB	28	90	1.29e-17	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000152923

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000153595

Predicted Effect

silent
Transcript: ENSMUST00000168483

SMART Domains

Protein: ENSMUSP00000126879
Gene: ENSMUSG00000066007

Domain	Start	End	E-Value	Type
KRAB	28	90	1.29e-17	SMART
ZnF_C2H2	251	273	7.9e-4	SMART
ZnF_C2H2	279	301	7.9e-4	SMART
ZnF_C2H2	307	329	3.49e-5	SMART
ZnF_C2H2	335	357	1.95e-3	SMART
ZnF_C2H2	363	385	7.9e-4	SMART
ZnF_C2H2	391	413	7.9e-4	SMART
ZnF_C2H2	419	441	3.49e-5	SMART
ZnF_C2H2	447	469	3.89e-3	SMART
ZnF_C2H2	475	497	2.75e-3	SMART
ZnF_C2H2	503	525	3.04e-5	SMART
ZnF_C2H2	531	553	8.34e-3	SMART
ZnF_C2H2	559	581	5.67e-5	SMART
ZnF_C2H2	587	609	1.98e-4	SMART
ZnF_C2H2	615	637	5.29e-5	SMART
ZnF_C2H2	643	665	1.58e-3	SMART
ZnF_C2H2	671	693	1.28e-3	SMART
ZnF_C2H2	699	721	1.6e-4	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000181199

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.0%
20x: 94.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 39 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abhd12b	T	A	12: 70,210,142 (GRCm39)		probably null	Het
Adamtsl5	C	T	10: 80,181,192 (GRCm39)	G63D	probably damaging	Het
Armc9	T	A	1: 86,127,569 (GRCm39)	M68K	probably benign	Het
Atad2	A	C	15: 57,971,733 (GRCm39)	H381Q	possibly damaging	Het
Bclaf1	T	G	10: 20,208,130 (GRCm39)		probably benign	Het
Ccdc188	A	G	16: 18,037,038 (GRCm39)	E238G	probably damaging	Het
Cldn19	G	T	4: 119,112,930 (GRCm39)	C54F	probably damaging	Het
Clip1	T	C	5: 123,785,946 (GRCm39)	D246G	probably damaging	Het
Col20a1	T	C	2: 180,640,379 (GRCm39)	V512A	probably damaging	Het
Cyfip1	G	T	7: 55,541,779 (GRCm39)	A355S	probably benign	Het
Dffa	T	G	4: 149,188,760 (GRCm39)	L18R	probably benign	Het
Dnah8	G	A	17: 31,009,891 (GRCm39)	E3761K	probably damaging	Het
Dusp10	A	G	1: 183,769,210 (GRCm39)	T59A	possibly damaging	Het
Eed	T	C	7: 89,626,173 (GRCm39)	S67G	probably benign	Het
Fam181a	C	T	12: 103,282,392 (GRCm39)	A99V	probably benign	Het
Gm12185	G	A	11: 48,799,044 (GRCm39)	T483I	possibly damaging	Het
Htr3b	A	T	9: 48,848,542 (GRCm39)	C234*	probably null	Het
Kidins220	C	A	12: 25,053,009 (GRCm39)	T433K	probably benign	Het
Man2a1	T	C	17: 64,943,502 (GRCm39)	Y186H	probably damaging	Het
Mcm9	G	T	10: 53,506,093 (GRCm39)	S60R	possibly damaging	Het
Mst1r	T	A	9: 107,784,773 (GRCm39)	C144S	probably damaging	Het
Nckap5	A	G	1: 125,955,461 (GRCm39)	C364R	probably damaging	Het
Nptx2	T	C	5: 144,493,041 (GRCm39)	I376T	probably damaging	Het
Or2a14	T	C	6: 43,130,961 (GRCm39)	S241P	probably damaging	Het
Otogl	A	T	10: 107,604,834 (GRCm39)	C2191S	probably damaging	Het
Plxdc2	T	A	2: 16,655,026 (GRCm39)	F208L	probably damaging	Het
Robo3	A	C	9: 37,328,175 (GRCm39)	Y1339D	probably damaging	Het
Rsph9	G	T	17: 46,446,008 (GRCm39)	Y42*	probably null	Het
Slc39a1	T	A	3: 90,156,702 (GRCm39)	L86Q	probably null	Het
Slfn8	T	C	11: 82,904,214 (GRCm39)	D392G	probably damaging	Het
Tiprl	A	G	1: 165,043,337 (GRCm39)	V263A	probably benign	Het
Tmub2	A	G	11: 102,175,820 (GRCm39)		probably benign	Het
Trpm1	T	A	7: 63,918,702 (GRCm39)	F681I	probably damaging	Het
Ttn	A	T	2: 76,641,587 (GRCm39)	L5176Q	possibly damaging	Het
Uba3	A	T	6: 97,178,896 (GRCm39)	C68*	probably null	Het
Vmn1r158	T	A	7: 22,489,799 (GRCm39)	M137L	probably benign	Het
Vmn1r50	C	T	6: 90,084,465 (GRCm39)	A70V	possibly damaging	Het
Wwc1	T	C	11: 35,766,723 (GRCm39)	K511E	probably benign	Het
Zng1	G	T	19: 24,897,994 (GRCm39)	T382K	probably damaging	Het

Other mutations in Zfp600

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
PIT4131001:Zfp600	UTSW	4	146,131,802 (GRCm39)	frame shift	probably null
R1004:Zfp600	UTSW	4	146,133,103 (GRCm39)	unclassified	probably benign
R1323:Zfp600	UTSW	4	146,133,261 (GRCm39)	missense	probably damaging	1.00
R1323:Zfp600	UTSW	4	146,133,261 (GRCm39)	missense	probably damaging	1.00
R1702:Zfp600	UTSW	4	146,133,497 (GRCm39)	missense	probably benign	0.04
R2165:Zfp600	UTSW	4	146,133,488 (GRCm39)	nonsense	probably null
R5548:Zfp600	UTSW	4	146,133,019 (GRCm39)	missense	possibly damaging	0.86
R5646:Zfp600	UTSW	4	146,131,670 (GRCm39)	missense	probably damaging	1.00
R5948:Zfp600	UTSW	4	146,131,645 (GRCm39)	missense	probably damaging	0.96
R6112:Zfp600	UTSW	4	146,131,701 (GRCm39)	missense	probably benign	0.41
R7044:Zfp600	UTSW	4	146,131,892 (GRCm39)	nonsense	probably null
R7836:Zfp600	UTSW	4	146,133,523 (GRCm39)	missense	probably benign
R8080:Zfp600	UTSW	4	146,133,182 (GRCm39)	missense	unknown
R8749:Zfp600	UTSW	4	146,133,151 (GRCm39)	missense	unknown
R9255:Zfp600	UTSW	4	146,131,673 (GRCm39)	missense	possibly damaging	0.70
Z1176:Zfp600	UTSW	4	146,133,209 (GRCm39)	missense	unknown
Z1177:Zfp600	UTSW	4	146,133,333 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TCACTGCATATGTGGAAACTATGAG -3'
(R):5'- GACTTTGAAGATTGTCACTCTGAG -3'

Sequencing Primer
(F):5'- CTATGAGAAGGAGAAGGTCTTGGAAC -3'
(R):5'- GTCACTCTGAGTAAAGCATTTGTC -3'

Posted On

2016-07-06