Mutagenetix > Incidental Mutations

Incidental Mutation 'R5199:Olfr203'

400634

Institutional Source

Beutler Lab

Gene Symbol

Olfr203

Ensembl Gene

ENSMUSG00000068182

Gene Name

olfactory receptor 203

Synonyms

MOR182-5, GA_x54KRFPKG5P-55517445-55518365

MMRRC Submission

042775-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.087) question?

Stock #

R5199 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

59298881-59304413 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 59303740 bp

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Isoleucine at position 196 (F196I)

Ref Sequence

ENSEMBL: ENSMUSP00000149906 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000089305] [ENSMUST00000201687] [ENSMUST00000215893]

Predicted Effect

probably benign
Transcript: ENSMUST00000089305
AA Change: F197I

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000086719
Gene: ENSMUSG00000068182
AA Change: F197I

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	307	1e-46	PFAM
Pfam:7tm_1	41	290	7.6e-20	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000201687
AA Change: F196I

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000144622
Gene: ENSMUSG00000048810
AA Change: F196I

Domain	Start	End	E-Value	Type
Pfam:7tm_4	30	306	3.4e-46	PFAM
Pfam:7tm_1	40	289	1.4e-19	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000215893
AA Change: F196I

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.4%
20x: 95.7%

Validation Efficiency

100% (57/57)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam1a	T	C	5: 121,521,152	E26G	probably benign	Het
Adar	T	A	3: 89,745,944	M797K	probably damaging	Het
Amdhd1	A	T	10: 93,525,985	C352S	probably damaging	Het
AW554918	C	A	18: 25,340,299	R387S	probably damaging	Het
C130079G13Rik	T	C	3: 59,936,485	L200P	probably damaging	Het
Cabp1	A	G	5: 115,186,043	V5A	possibly damaging	Het
Carmil1	A	G	13: 24,111,870	L387P	probably damaging	Het
Cds2	T	A	2: 132,298,483	H200Q	probably damaging	Het
Cep170b	T	A	12: 112,744,147	L1470Q	probably damaging	Het
Cnnm1	A	G	19: 43,494,986	D956G	possibly damaging	Het
Cnot8	C	T	11: 58,115,274	Q210*	probably null	Het
Cpne8	G	A	15: 90,648,609	T65I	probably benign	Het
Crygn	A	G	5: 24,756,158	V50A	probably damaging	Het
Cxcr4	A	G	1: 128,589,546	V126A	probably damaging	Het
Cyp4f15	A	G	17: 32,702,372	D464G	probably benign	Het
Dapp1	C	T	3: 137,981,385	S12N	probably benign	Het
Dhps	G	A	8: 85,073,406	G162R	probably damaging	Het
Dsp	T	G	13: 38,192,902	Y1554*	probably null	Het
Etl4	G	T	2: 20,744,042	R397L	probably damaging	Het
Ezh2	C	T	6: 47,551,725	C291Y	probably benign	Het
Gbp9	T	C	5: 105,083,812	S303G	probably benign	Het
Gm11444	A	C	11: 85,848,019	S83A	unknown	Het
Gm156	T	A	6: 129,775,818	Y8F	possibly damaging	Het
Gpat4	A	G	8: 23,182,696	V46A	possibly damaging	Het
Haus6	T	C	4: 86,582,985	D883G	possibly damaging	Het
Hinfp	T	C	9: 44,296,392	E439G	probably benign	Het
Ifna14	T	A	4: 88,571,362	Y146F	probably damaging	Het
Igkv3-3	A	T	6: 70,687,504	Y110F	probably damaging	Het
Kansl2-ps	A	G	7: 72,673,194		noncoding transcript	Het
Mcmdc2	T	C	1: 9,920,435	V279A	probably benign	Het
Mug2	T	A	6: 122,040,660	V452D	probably benign	Het
Ndufb3	C	G	1: 58,591,122		probably benign	Het
Oas1d	G	T	5: 120,919,145	K271N	probably benign	Het
Olfr1012	A	G	2: 85,760,214	L54P	probably damaging	Het
Olfr1215	T	A	2: 89,001,763	H175L	possibly damaging	Het
Otx1	C	A	11: 21,997,037	A91S	probably damaging	Het
Pcnt	T	A	10: 76,418,544	H817L	probably benign	Het
Per3	G	T	4: 151,012,895	S724R	probably benign	Het
Phlpp1	T	A	1: 106,173,394	V464E	probably damaging	Het
Psme4	A	G	11: 30,853,272	E38G	probably benign	Het
Qtrt2	T	C	16: 43,867,425	N264S	probably benign	Het
Ranbp2	G	A	10: 58,464,443	R557H	probably benign	Het
Rptor	T	A	11: 119,603,816	S3T	probably benign	Het
Saxo1	T	A	4: 86,487,782	Y60F	probably damaging	Het
St3gal1	A	G	15: 67,113,715	V30A	probably benign	Het
Tmem245	G	A	4: 56,925,149	S324L	probably benign	Het
Topbp1	A	G	9: 103,346,672		probably benign	Het
Urb1	T	C	16: 90,792,748	T382A	possibly damaging	Het
Vmn1r178	A	T	7: 23,894,389	L214F	probably benign	Het
Vmn2r82	G	A	10: 79,396,087	C640Y	probably damaging	Het
Vsx2	A	G	12: 84,593,210	D281G	probably benign	Het
Zfp804b	A	T	5: 6,770,013	C1017S	probably benign	Het

Other mutations in Olfr203

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02003:Olfr203	APN	16	59303633	missense	probably damaging	0.99
IGL02294:Olfr203	APN	16	59303612	missense	probably damaging	1.00
IGL02412:Olfr203	APN	16	59303192	missense	probably damaging	1.00
IGL02899:Olfr203	APN	16	59303286	missense	probably damaging	1.00
R0792:Olfr203	UTSW	16	59303989	missense	probably damaging	0.99
R1551:Olfr203	UTSW	16	59303403	missense	probably benign	0.03
R1701:Olfr203	UTSW	16	59303288	missense	probably benign	0.23
R1975:Olfr203	UTSW	16	59303728	missense	probably damaging	0.98
R2272:Olfr203	UTSW	16	59303444	missense	possibly damaging	0.55
R5843:Olfr203	UTSW	16	59303361	missense	probably damaging	1.00
R5928:Olfr203	UTSW	16	59303158	missense	probably damaging	1.00
R6708:Olfr203	UTSW	16	59304053	missense	probably damaging	1.00
R6747:Olfr203	UTSW	16	59303641	missense	probably benign	0.03
R6894:Olfr203	UTSW	16	59303779	missense	probably damaging	0.98
R7324:Olfr203	UTSW	16	59303248	missense	probably benign
R7380:Olfr203	UTSW	16	59304028	missense	probably damaging	1.00
R7612:Olfr203	UTSW	16	59303627	missense	probably damaging	1.00
R7775:Olfr203	UTSW	16	59303251	missense	probably damaging	1.00
R8010:Olfr203	UTSW	16	59303504	missense	probably damaging	1.00
Z1176:Olfr203	UTSW	16	59303169	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- GCTATGTAGCCATATGCAATCC -3'
(R):5'- CCAGGGCTCACGTACATAAAG -3'

Sequencing Primer
(F):5'- ATGTCCAACAGAGTGTGCACTTG -3'
(R):5'- TCACGTACATAAAGAAGAGAGTTCC -3'

Posted On

2016-07-06