Mutagenetix > Incidental Mutation

Incidental Mutation 'R5202:Nbeal2'

400915

Institutional Source

Beutler Lab

Gene Symbol

Nbeal2

Ensembl Gene

ENSMUSG00000056724

Gene Name

neurobeachin-like 2

Synonyms

1110014F23Rik

MMRRC Submission

042777-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.228) question?

Stock #

R5202 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

110624789-110654161 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 110644666 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Glycine at position 28 (E28G)

Ref Sequence

ENSEMBL: ENSMUSP00000119254 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000133191] [ENSMUST00000149089] [ENSMUST00000167320] [ENSMUST00000196488]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000133191
AA Change: E35G

PolyPhen 2 Score 0.903 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000121373
Gene: ENSMUSG00000056724
AA Change: E35G

Domain	Start	End	E-Value	Type
low complexity region	56	84	N/A	INTRINSIC
low complexity region	192	201	N/A	INTRINSIC
low complexity region	227	238	N/A	INTRINSIC
low complexity region	514	522	N/A	INTRINSIC
low complexity region	527	542	N/A	INTRINSIC
Pfam:Laminin_G_3	578	818	5.9e-8	PFAM
low complexity region	1014	1028	N/A	INTRINSIC
low complexity region	1128	1136	N/A	INTRINSIC
low complexity region	1149	1163	N/A	INTRINSIC
low complexity region	1359	1375	N/A	INTRINSIC
low complexity region	1515	1530	N/A	INTRINSIC
low complexity region	1621	1647	N/A	INTRINSIC
low complexity region	1875	1904	N/A	INTRINSIC
Pfam:PH_BEACH	1908	2002	6.2e-28	PFAM
Beach	2054	2334	5.2e-205	SMART
WD40	2432	2477	1.03e1	SMART
WD40	2480	2519	6.19e-5	SMART
WD40	2531	2570	1.02e-5	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000149089
AA Change: E28G

PolyPhen 2 Score 0.993 (Sensitivity: 0.70; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000119254
Gene: ENSMUSG00000056724
AA Change: E28G

Domain	Start	End	E-Value	Type
low complexity region	49	77	N/A	INTRINSIC
low complexity region	185	194	N/A	INTRINSIC
low complexity region	220	231	N/A	INTRINSIC
low complexity region	480	488	N/A	INTRINSIC
low complexity region	493	508	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000167320
AA Change: E35G

PolyPhen 2 Score 0.916 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000128586
Gene: ENSMUSG00000056724
AA Change: E35G

Domain	Start	End	E-Value	Type
low complexity region	56	84	N/A	INTRINSIC
low complexity region	192	201	N/A	INTRINSIC
low complexity region	227	238	N/A	INTRINSIC
low complexity region	514	522	N/A	INTRINSIC
low complexity region	527	542	N/A	INTRINSIC
low complexity region	763	775	N/A	INTRINSIC
Pfam:DUF4704	872	1148	9.2e-32	PFAM
low complexity region	1149	1163	N/A	INTRINSIC
low complexity region	1366	1382	N/A	INTRINSIC
low complexity region	1522	1537	N/A	INTRINSIC
Pfam:DUF4800	1590	1856	1.5e-112	PFAM
low complexity region	1882	1911	N/A	INTRINSIC
Pfam:PH_BEACH	1922	2009	3.1e-21	PFAM
Beach	2061	2341	5.2e-205	SMART
WD40	2439	2484	1.03e1	SMART
WD40	2487	2526	6.19e-5	SMART
WD40	2538	2577	1.02e-5	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000196488
AA Change: E35G

PolyPhen 2 Score 0.932 (Sensitivity: 0.80; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000143265
Gene: ENSMUSG00000056724
AA Change: E35G

Domain	Start	End	E-Value	Type
low complexity region	56	84	N/A	INTRINSIC
low complexity region	192	201	N/A	INTRINSIC
low complexity region	227	238	N/A	INTRINSIC
low complexity region	487	495	N/A	INTRINSIC
low complexity region	500	515	N/A	INTRINSIC
Pfam:Laminin_G_3	551	791	5.3e-6	PFAM
low complexity region	987	1001	N/A	INTRINSIC
low complexity region	1101	1109	N/A	INTRINSIC
low complexity region	1122	1136	N/A	INTRINSIC
low complexity region	1332	1348	N/A	INTRINSIC
low complexity region	1488	1503	N/A	INTRINSIC
low complexity region	1594	1620	N/A	INTRINSIC
low complexity region	1848	1877	N/A	INTRINSIC
Pfam:PH_BEACH	1881	1975	3.1e-25	PFAM
Beach	2027	2307	3.8e-209	SMART
WD40	2405	2450	6.3e-2	SMART
WD40	2453	2492	3.8e-7	SMART
WD40	2504	2543	6.5e-8	SMART

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.1%
20x: 94.9%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene contains a beige and Chediak-Higashi (BEACH) domain and multiple WD40 domains, and may play a role in megakaryocyte alpha-granule biogenesis. Mutations in this gene are a cause of gray platelet syndrome. [provided by RefSeq, Dec 2011]
PHENOTYPE: Homozygous null mice exhibit megakaryocyte and platelet abnormalities resulting in impaired arterial thrombus formation and protection from infarction following cerebral ischemia. Wound repair is impaired. These abnormalities result in a bleeding disorder similiar to Gray Platelet Syndrome. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4932414N04Rik	A	T	2: 68,731,964 (GRCm38)	M312L	probably benign	Het
Adam7	C	A	14: 68,507,856 (GRCm38)	D640Y	possibly damaging	Het
Alcam	T	C	16: 52,274,236 (GRCm38)	Y384C	probably damaging	Het
Apob	A	G	12: 8,013,737 (GRCm38)	E289G	probably damaging	Het
Arhgap15	A	T	2: 44,063,857 (GRCm38)	R198S	probably benign	Het
Bbs2	G	T	8: 94,092,414 (GRCm38)	S109*	probably null	Het
Bfsp1	T	C	2: 143,826,971 (GRCm38)	S569G	probably benign	Het
Chst9	A	T	18: 15,453,239 (GRCm38)	I89N	probably benign	Het
Cntln	A	C	4: 84,971,229 (GRCm38)	E316D	probably benign	Het
Csde1	T	G	3: 103,039,934 (GRCm38)	D67E	probably damaging	Het
Csf2rb	T	G	15: 78,349,057 (GRCm38)	S855A	possibly damaging	Het
Cyp4a10	T	A	4: 115,532,615 (GRCm38)	D472E	probably damaging	Het
Cyp4f18	C	A	8: 72,009,096 (GRCm38)	R49L	probably benign	Het
Daam1	G	T	12: 71,944,274 (GRCm38)	V221L	unknown	Het
Dbr1	T	G	9: 99,583,891 (GRCm38)	D507E	probably benign	Het
Dhrs7c	A	G	11: 67,815,801 (GRCm38)	M188V	probably benign	Het
Dicer1	A	T	12: 104,694,731 (GRCm38)	L1688*	probably null	Het
Dmpk	C	G	7: 19,088,019 (GRCm38)	L301V	probably benign	Het
E2f3	G	A	13: 29,918,636 (GRCm38)	T91I	probably damaging	Het
Etaa1	A	T	11: 17,947,853 (GRCm38)	V157E	probably damaging	Het
Foxp2	A	T	6: 15,394,771 (GRCm38)	T157S	probably benign	Het
Frem2	A	G	3: 53,551,346 (GRCm38)	V2034A	probably benign	Het
Fzd10	T	C	5: 128,602,116 (GRCm38)	V300A	possibly damaging	Het
Gbf1	A	T	19: 46,268,454 (GRCm38)	M778L	probably benign	Het
Gm17472	T	A	6: 42,981,134 (GRCm38)	N112K	probably benign	Het
Gria4	T	C	9: 4,424,330 (GRCm38)	K845R	probably benign	Het
Hspa4l	T	A	3: 40,781,569 (GRCm38)	S541T	probably benign	Het
Igkv4-68	G	A	6: 69,304,942 (GRCm38)	R82C	probably damaging	Het
Itgb1	A	T	8: 128,720,010 (GRCm38)	I383F	probably damaging	Het
Kcnk12	T	A	17: 87,746,605 (GRCm38)	K210*	probably null	Het
Limch1	C	G	5: 66,993,173 (GRCm38)	D163E	probably damaging	Het
Mbd4	A	T	6: 115,849,402 (GRCm38)	D188E	probably damaging	Het
Myh9	C	T	15: 77,781,110 (GRCm38)		probably null	Het
Nfatc4	A	G	14: 55,826,659 (GRCm38)	E201G	probably damaging	Het
Nrap	G	A	19: 56,335,151 (GRCm38)	H1330Y	probably damaging	Het
Nudt14	A	G	12: 112,935,028 (GRCm38)	S151P	probably damaging	Het
Olfr297	C	A	7: 86,527,116 (GRCm38)	R120S	probably damaging	Het
Olfr58	T	A	9: 19,783,218 (GRCm38)	F28L	possibly damaging	Het
Olfr706	T	A	7: 106,886,596 (GRCm38)	T74S	possibly damaging	Het
Otx1	C	A	11: 21,997,037 (GRCm38)	A91S	probably damaging	Het
Paqr9	A	G	9: 95,560,110 (GRCm38)	D51G	probably damaging	Het
Pcgf5	T	A	19: 36,437,183 (GRCm38)	F80I	probably damaging	Het
Pkhd1	T	G	1: 20,547,341 (GRCm38)	S1007R	probably benign	Het
Rab30	T	C	7: 92,835,913 (GRCm38)	Y196H	probably benign	Het
Setd2	A	T	9: 110,551,230 (GRCm38)	D1371V	probably damaging	Het
Slc48a1	A	T	15: 97,790,700 (GRCm38)	I140F	possibly damaging	Het
Sorl1	A	G	9: 42,033,583 (GRCm38)	V882A	probably benign	Het
Sptbn2	G	T	19: 4,724,184 (GRCm38)	G88W	probably damaging	Het
Tdrd12	A	G	7: 35,490,030 (GRCm38)	V427A	possibly damaging	Het
Tekt2	T	C	4: 126,324,670 (GRCm38)	D69G	probably benign	Het
Tie1	C	T	4: 118,480,510 (GRCm38)	V463I	probably benign	Het
Tmem150c	G	T	5: 100,079,954 (GRCm38)	H217N	probably damaging	Het
Tnks2	G	A	19: 36,888,852 (GRCm38)	G1080R	probably damaging	Het
Ttll4	G	A	1: 74,687,852 (GRCm38)		probably null	Het

Other mutations in Nbeal2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00229:Nbeal2	APN	9	110,635,869 (GRCm38)	missense	probably damaging	1.00
IGL00784:Nbeal2	APN	9	110,629,763 (GRCm38)	splice site	probably benign
IGL00826:Nbeal2	APN	9	110,626,903 (GRCm38)	missense	probably benign
IGL00885:Nbeal2	APN	9	110,638,661 (GRCm38)	missense	probably damaging	1.00
IGL01348:Nbeal2	APN	9	110,629,146 (GRCm38)	missense	probably damaging	0.99
IGL01511:Nbeal2	APN	9	110,629,234 (GRCm38)	missense	probably damaging	1.00
IGL01571:Nbeal2	APN	9	110,632,758 (GRCm38)	missense	possibly damaging	0.63
IGL01612:Nbeal2	APN	9	110,644,678 (GRCm38)	missense	probably damaging	1.00
IGL01924:Nbeal2	APN	9	110,631,414 (GRCm38)	missense	probably benign	0.23
IGL02056:Nbeal2	APN	9	110,627,324 (GRCm38)	missense	probably benign	0.17
IGL02481:Nbeal2	APN	9	110,625,995 (GRCm38)	nonsense	probably null
IGL02483:Nbeal2	APN	9	110,625,995 (GRCm38)	nonsense	probably null
IGL02502:Nbeal2	APN	9	110,633,768 (GRCm38)	missense	probably damaging	1.00
IGL02631:Nbeal2	APN	9	110,630,208 (GRCm38)	missense	probably damaging	0.99
IGL02637:Nbeal2	APN	9	110,625,977 (GRCm38)	missense	possibly damaging	0.62
IGL02727:Nbeal2	APN	9	110,639,285 (GRCm38)	splice site	probably benign
IGL02887:Nbeal2	APN	9	110,628,276 (GRCm38)	missense	probably damaging	1.00
IGL02896:Nbeal2	APN	9	110,639,292 (GRCm38)	critical splice donor site	probably null
IGL03110:Nbeal2	APN	9	110,631,433 (GRCm38)	missense	probably damaging	1.00
Antonym	UTSW	9	110,630,252 (GRCm38)	missense	probably damaging	1.00
Beowulf	UTSW	9	110,637,937 (GRCm38)	missense	possibly damaging	0.65
Blackmail	UTSW	9	110,629,639 (GRCm38)	missense	probably damaging	1.00
dog	UTSW	9	110,635,341 (GRCm38)	missense	possibly damaging	0.89
extortion	UTSW	9	110,630,243 (GRCm38)	missense	probably damaging	1.00
legion	UTSW	9	110,629,179 (GRCm38)	missense	probably damaging	1.00
litigious	UTSW	9	110,628,195 (GRCm38)	missense	probably damaging	1.00
mall	UTSW	9	110,632,886 (GRCm38)	missense	probably damaging	1.00
Mollusca	UTSW	9	110,645,438 (GRCm38)	splice site	probably null
Schleuter	UTSW	9	110,628,744 (GRCm38)	missense	possibly damaging	0.69
shellfish	UTSW	9	110,628,720 (GRCm38)	missense	probably damaging	1.00
Sophomoric	UTSW	9	110,633,047 (GRCm38)	missense	probably damaging	1.00
F5770:Nbeal2	UTSW	9	110,637,937 (GRCm38)	missense	possibly damaging	0.65
R0032:Nbeal2	UTSW	9	110,637,868 (GRCm38)	splice site	probably benign
R0084:Nbeal2	UTSW	9	110,643,710 (GRCm38)	critical splice donor site	probably null
R0147:Nbeal2	UTSW	9	110,642,143 (GRCm38)	nonsense	probably null
R0294:Nbeal2	UTSW	9	110,632,859 (GRCm38)	missense	probably damaging	1.00
R0310:Nbeal2	UTSW	9	110,638,163 (GRCm38)	missense	probably damaging	1.00
R0494:Nbeal2	UTSW	9	110,627,187 (GRCm38)	missense	probably damaging	1.00
R0550:Nbeal2	UTSW	9	110,642,158 (GRCm38)	missense	probably benign	0.01
R0630:Nbeal2	UTSW	9	110,636,034 (GRCm38)	splice site	probably benign
R0762:Nbeal2	UTSW	9	110,643,808 (GRCm38)	splice site	probably benign
R0862:Nbeal2	UTSW	9	110,628,195 (GRCm38)	missense	probably damaging	1.00
R0864:Nbeal2	UTSW	9	110,628,195 (GRCm38)	missense	probably damaging	1.00
R1225:Nbeal2	UTSW	9	110,632,886 (GRCm38)	missense	probably damaging	1.00
R1240:Nbeal2	UTSW	9	110,627,108 (GRCm38)	missense	probably damaging	0.98
R1450:Nbeal2	UTSW	9	110,633,672 (GRCm38)	splice site	probably benign
R1519:Nbeal2	UTSW	9	110,636,305 (GRCm38)	missense	probably damaging	1.00
R1655:Nbeal2	UTSW	9	110,632,872 (GRCm38)	missense	probably damaging	1.00
R1668:Nbeal2	UTSW	9	110,638,893 (GRCm38)	missense	probably damaging	1.00
R1705:Nbeal2	UTSW	9	110,625,196 (GRCm38)	missense	probably damaging	1.00
R1784:Nbeal2	UTSW	9	110,630,857 (GRCm38)	nonsense	probably null
R1834:Nbeal2	UTSW	9	110,627,129 (GRCm38)	missense	probably damaging	1.00
R1997:Nbeal2	UTSW	9	110,632,198 (GRCm38)	missense	probably damaging	1.00
R2013:Nbeal2	UTSW	9	110,634,071 (GRCm38)	missense	probably benign	0.09
R2014:Nbeal2	UTSW	9	110,634,071 (GRCm38)	missense	probably benign	0.09
R2055:Nbeal2	UTSW	9	110,635,307 (GRCm38)	missense	possibly damaging	0.92
R2086:Nbeal2	UTSW	9	110,634,071 (GRCm38)	missense	probably benign	0.09
R2113:Nbeal2	UTSW	9	110,625,406 (GRCm38)	missense	probably damaging	1.00
R2167:Nbeal2	UTSW	9	110,638,308 (GRCm38)	missense	probably damaging	1.00
R2201:Nbeal2	UTSW	9	110,630,250 (GRCm38)	missense	probably benign	0.16
R2309:Nbeal2	UTSW	9	110,626,570 (GRCm38)	missense	probably damaging	1.00
R2378:Nbeal2	UTSW	9	110,630,808 (GRCm38)	missense	probably damaging	0.99
R2945:Nbeal2	UTSW	9	110,628,068 (GRCm38)	missense	possibly damaging	0.82
R3052:Nbeal2	UTSW	9	110,633,085 (GRCm38)	missense	possibly damaging	0.93
R3076:Nbeal2	UTSW	9	110,631,700 (GRCm38)	missense	probably damaging	1.00
R3176:Nbeal2	UTSW	9	110,636,887 (GRCm38)	splice site	probably benign
R3974:Nbeal2	UTSW	9	110,633,846 (GRCm38)	missense	probably damaging	1.00
R4183:Nbeal2	UTSW	9	110,636,675 (GRCm38)	missense	probably benign
R4342:Nbeal2	UTSW	9	110,631,793 (GRCm38)	intron	probably benign
R4654:Nbeal2	UTSW	9	110,632,004 (GRCm38)	missense	probably damaging	1.00
R4707:Nbeal2	UTSW	9	110,632,055 (GRCm38)	missense	probably benign	0.10
R4822:Nbeal2	UTSW	9	110,636,315 (GRCm38)	missense	possibly damaging	0.82
R4854:Nbeal2	UTSW	9	110,631,396 (GRCm38)	missense	probably damaging	1.00
R4860:Nbeal2	UTSW	9	110,635,194 (GRCm38)	missense	probably benign	0.00
R4860:Nbeal2	UTSW	9	110,635,194 (GRCm38)	missense	probably benign	0.00
R4990:Nbeal2	UTSW	9	110,634,803 (GRCm38)	missense	probably benign	0.10
R4991:Nbeal2	UTSW	9	110,638,767 (GRCm38)	missense	probably damaging	1.00
R5021:Nbeal2	UTSW	9	110,637,463 (GRCm38)	missense	probably damaging	0.99
R5057:Nbeal2	UTSW	9	110,631,005 (GRCm38)	missense	probably damaging	1.00
R5092:Nbeal2	UTSW	9	110,626,728 (GRCm38)	splice site	probably null
R5161:Nbeal2	UTSW	9	110,629,868 (GRCm38)	missense	probably benign
R5217:Nbeal2	UTSW	9	110,632,090 (GRCm38)	missense	possibly damaging	0.56
R5408:Nbeal2	UTSW	9	110,637,520 (GRCm38)	missense	possibly damaging	0.91
R5540:Nbeal2	UTSW	9	110,631,733 (GRCm38)	missense	probably damaging	1.00
R5866:Nbeal2	UTSW	9	110,631,492 (GRCm38)	missense	probably damaging	1.00
R5925:Nbeal2	UTSW	9	110,629,880 (GRCm38)	missense	probably benign	0.00
R6057:Nbeal2	UTSW	9	110,641,877 (GRCm38)	missense	possibly damaging	0.81
R6180:Nbeal2	UTSW	9	110,625,147 (GRCm38)	missense	probably damaging	1.00
R6191:Nbeal2	UTSW	9	110,627,990 (GRCm38)	critical splice donor site	probably null
R6232:Nbeal2	UTSW	9	110,638,734 (GRCm38)	missense	probably damaging	1.00
R6372:Nbeal2	UTSW	9	110,628,744 (GRCm38)	missense	possibly damaging	0.69
R6423:Nbeal2	UTSW	9	110,625,994 (GRCm38)	missense	probably damaging	1.00
R6543:Nbeal2	UTSW	9	110,644,458 (GRCm38)	missense	probably benign
R6648:Nbeal2	UTSW	9	110,637,642 (GRCm38)	missense	probably damaging	1.00
R6722:Nbeal2	UTSW	9	110,632,992 (GRCm38)	missense	probably damaging	1.00
R6738:Nbeal2	UTSW	9	110,636,905 (GRCm38)	missense	possibly damaging	0.93
R6916:Nbeal2	UTSW	9	110,626,108 (GRCm38)	missense	probably damaging	1.00
R6935:Nbeal2	UTSW	9	110,639,391 (GRCm38)	missense	probably damaging	1.00
R7022:Nbeal2	UTSW	9	110,638,618 (GRCm38)	missense	probably damaging	1.00
R7023:Nbeal2	UTSW	9	110,638,618 (GRCm38)	missense	probably damaging	1.00
R7050:Nbeal2	UTSW	9	110,628,720 (GRCm38)	missense	probably damaging	1.00
R7072:Nbeal2	UTSW	9	110,626,051 (GRCm38)	missense	probably benign	0.01
R7073:Nbeal2	UTSW	9	110,626,109 (GRCm38)	missense	probably damaging	0.99
R7099:Nbeal2	UTSW	9	110,645,438 (GRCm38)	splice site	probably null
R7354:Nbeal2	UTSW	9	110,629,179 (GRCm38)	missense	probably damaging	1.00
R7394:Nbeal2	UTSW	9	110,630,189 (GRCm38)	critical splice donor site	probably null
R7397:Nbeal2	UTSW	9	110,628,032 (GRCm38)	missense	possibly damaging	0.78
R7552:Nbeal2	UTSW	9	110,653,917 (GRCm38)	missense	probably benign	0.16
R7619:Nbeal2	UTSW	9	110,625,818 (GRCm38)	missense	probably benign	0.19
R7821:Nbeal2	UTSW	9	110,630,252 (GRCm38)	missense	probably damaging	1.00
R7902:Nbeal2	UTSW	9	110,637,547 (GRCm38)	missense	probably benign
R7923:Nbeal2	UTSW	9	110,631,446 (GRCm38)	nonsense	probably null
R8018:Nbeal2	UTSW	9	110,629,157 (GRCm38)	unclassified	probably benign
R8190:Nbeal2	UTSW	9	110,626,090 (GRCm38)	missense	probably benign	0.04
R8297:Nbeal2	UTSW	9	110,635,341 (GRCm38)	missense	possibly damaging	0.89
R8404:Nbeal2	UTSW	9	110,634,389 (GRCm38)	missense	possibly damaging	0.48
R8502:Nbeal2	UTSW	9	110,634,389 (GRCm38)	missense	possibly damaging	0.48
R8737:Nbeal2	UTSW	9	110,627,881 (GRCm38)	missense	probably damaging	1.00
R8782:Nbeal2	UTSW	9	110,630,805 (GRCm38)	missense	probably benign	0.04
R8807:Nbeal2	UTSW	9	110,629,639 (GRCm38)	missense	probably damaging	1.00
R8877:Nbeal2	UTSW	9	110,630,243 (GRCm38)	missense	probably damaging	1.00
R9057:Nbeal2	UTSW	9	110,627,150 (GRCm38)	missense	probably benign
R9267:Nbeal2	UTSW	9	110,633,047 (GRCm38)	missense	probably damaging	1.00
R9313:Nbeal2	UTSW	9	110,634,368 (GRCm38)	missense	probably damaging	1.00
R9352:Nbeal2	UTSW	9	110,627,848 (GRCm38)	missense	probably benign	0.03
R9482:Nbeal2	UTSW	9	110,633,998 (GRCm38)	missense	probably benign	0.25
R9533:Nbeal2	UTSW	9	110,644,661 (GRCm38)	missense	probably benign	0.01
R9566:Nbeal2	UTSW	9	110,628,921 (GRCm38)	missense	probably benign	0.00
R9769:Nbeal2	UTSW	9	110,626,279 (GRCm38)	missense	probably benign	0.01
V7583:Nbeal2	UTSW	9	110,637,937 (GRCm38)	missense	possibly damaging	0.65
X0017:Nbeal2	UTSW	9	110,644,278 (GRCm38)	missense	probably benign	0.02
X0065:Nbeal2	UTSW	9	110,644,413 (GRCm38)	splice site	probably benign
Z1088:Nbeal2	UTSW	9	110,632,372 (GRCm38)	missense	possibly damaging	0.51
Z1176:Nbeal2	UTSW	9	110,638,835 (GRCm38)	missense	probably benign
Z1176:Nbeal2	UTSW	9	110,625,816 (GRCm38)	missense	probably benign	0.01
Z1177:Nbeal2	UTSW	9	110,629,854 (GRCm38)	missense	probably benign	0.03

Predicted Primers

PCR Primer
(F):5'- ATGTAGAGCATCTAGGGGTAGC -3'
(R):5'- ACCACAGTCCTCTGGTTCTG -3'

Sequencing Primer
(F):5'- CTAGGGGTAGCAGAGGCACTTC -3'
(R):5'- TGACCTTCCACGTCCCGG -3'

Posted On

2016-07-06