Mutagenetix > Incidental Mutation

Incidental Mutation 'R5243:Rif1'

400966

Institutional Source

Beutler Lab

Gene Symbol

Rif1

Ensembl Gene

ENSMUSG00000036202

Gene Name

replication timing regulatory factor 1

Synonyms

6530403D07Rik, 5730435J01Rik, D2Ertd145e

MMRRC Submission

042814-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R5243 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

52072832-52122383 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 52111824 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Glutamine at position 1763 (H1763Q)

Ref Sequence

ENSEMBL: ENSMUSP00000108313 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000069794] [ENSMUST00000112693]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000069794
AA Change: H1763Q

PolyPhen 2 Score 0.862 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000064155
Gene: ENSMUSG00000036202
AA Change: H1763Q

Domain	Start	End	E-Value	Type
Pfam:Rif1_N	22	368	3.3e-78	PFAM
low complexity region	432	444	N/A	INTRINSIC
low complexity region	586	598	N/A	INTRINSIC
low complexity region	1018	1038	N/A	INTRINSIC
low complexity region	1180	1205	N/A	INTRINSIC
low complexity region	1310	1321	N/A	INTRINSIC
low complexity region	1423	1446	N/A	INTRINSIC
low complexity region	1576	1586	N/A	INTRINSIC
low complexity region	1677	1690	N/A	INTRINSIC
low complexity region	1702	1712	N/A	INTRINSIC
low complexity region	2176	2195	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000112693
AA Change: H1763Q

PolyPhen 2 Score 0.862 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000108313
Gene: ENSMUSG00000036202
AA Change: H1763Q

Domain	Start	End	E-Value	Type
Pfam:Rif1_N	18	381	1.4e-85	PFAM
low complexity region	432	444	N/A	INTRINSIC
low complexity region	586	598	N/A	INTRINSIC
low complexity region	1018	1038	N/A	INTRINSIC
low complexity region	1180	1205	N/A	INTRINSIC
low complexity region	1310	1321	N/A	INTRINSIC
low complexity region	1423	1446	N/A	INTRINSIC
low complexity region	1576	1586	N/A	INTRINSIC
low complexity region	1677	1690	N/A	INTRINSIC
low complexity region	1702	1712	N/A	INTRINSIC
low complexity region	2176	2195	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000125322
AA Change: H280Q

Predicted Effect

unknown
Transcript: ENSMUST00000125376
AA Change: H121Q

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000145130

Predicted Effect

probably benign
Transcript: ENSMUST00000152178

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.1%
20x: 95.0%

Validation Efficiency

95% (62/65)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that shares homology with the yeast teleomere binding protein, Rap1 interacting factor 1. This protein localizes to aberrant telomeres may be involved in DNA repair. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit embryonic lethality. Mice homozygous for a gene trap allele exhibit embryonic and postnatal lethality, reduced fertility, and decreased cell proliferation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930447C04Rik	A	T	12: 72,909,769 (GRCm38)		probably null	Het
Aatk	A	G	11: 120,016,768 (GRCm38)	L78P	probably damaging	Het
Acadsb	T	A	7: 131,444,243 (GRCm38)	I412K	probably damaging	Het
Acer3	T	C	7: 98,218,070 (GRCm38)	R246G	possibly damaging	Het
Atp1a3	T	A	7: 24,994,569 (GRCm38)	I387F	probably damaging	Het
Atp6v1b2	A	T	8: 69,103,739 (GRCm38)	M300L	probably benign	Het
Aurkb	T	A	11: 69,045,926 (GRCm38)		probably benign	Het
Card10	C	A	15: 78,780,672 (GRCm38)	G694V	possibly damaging	Het
Ccdc171	A	G	4: 83,604,107 (GRCm38)	N336S	probably damaging	Het
Copg2	T	C	6: 30,750,627 (GRCm38)	T720A	probably benign	Het
Coro2a	A	T	4: 46,545,620 (GRCm38)	W272R	probably damaging	Het
Cpne6	T	A	14: 55,512,747 (GRCm38)	C112S	probably damaging	Het
Ddx21	A	G	10: 62,602,213 (GRCm38)	M1T	probably null	Het
Dohh	A	G	10: 81,387,369 (GRCm38)	R161G	probably benign	Het
Dsc1	A	C	18: 20,099,159 (GRCm38)	V264G	probably damaging	Het
E4f1	A	T	17: 24,447,318 (GRCm38)	C198S	probably damaging	Het
Evpl	T	A	11: 116,222,969 (GRCm38)	E1298D	probably damaging	Het
Grid2ip	A	T	5: 143,377,505 (GRCm38)	I246F	probably damaging	Het
H2-M1	A	G	17: 36,671,301 (GRCm38)	F136S	possibly damaging	Het
Hck	T	C	2: 153,144,492 (GRCm38)	V387A	probably damaging	Het
Hdac1	G	A	4: 129,516,853 (GRCm38)		probably benign	Het
Hydin	A	C	8: 110,505,748 (GRCm38)	Q1633P	possibly damaging	Het
Igkv12-41	A	G	6: 69,858,702 (GRCm38)	I22T	probably damaging	Het
Ints3	CA	CAA	3: 90,401,144 (GRCm38)		probably null	Het
Itpr2	C	G	6: 146,187,546 (GRCm38)	W2188C	probably damaging	Het
Kcnt1	A	G	2: 25,908,074 (GRCm38)	E899G	probably damaging	Het
Lgals8	C	A	13: 12,454,764 (GRCm38)	V12L	probably benign	Het
Lgr6	T	C	1: 135,109,272 (GRCm38)		probably benign	Het
Lrrc8b	T	G	5: 105,480,946 (GRCm38)	I386S	probably damaging	Het
Mks1	T	C	11: 87,856,678 (GRCm38)		probably benign	Het
Mon1b	A	G	8: 113,637,921 (GRCm38)	D110G	possibly damaging	Het
Mpdz	G	T	4: 81,306,879 (GRCm38)	A1437E	probably damaging	Het
Nbeal1	T	A	1: 60,270,328 (GRCm38)	N1753K	probably damaging	Het
Nceh1	T	C	3: 27,241,688 (GRCm38)	V366A	probably damaging	Het
Ncor1	A	T	11: 62,338,962 (GRCm38)	D893E	probably damaging	Het
Npc1	T	A	18: 12,198,631 (GRCm38)		probably benign	Het
Olfr1505	A	G	19: 13,919,661 (GRCm38)	I214V	probably damaging	Het
Olfr344	T	C	2: 36,568,643 (GRCm38)	L15P	probably damaging	Het
Podxl2	T	C	6: 88,849,374 (GRCm38)	T317A	probably benign	Het
Prl8a9	T	G	13: 27,562,463 (GRCm38)	Q54P	probably damaging	Het
Prpf31	C	T	7: 3,638,754 (GRCm38)	R351*	probably null	Het
Pspc1	C	T	14: 56,764,191 (GRCm38)	G242D	probably damaging	Het
Rbmxl2	G	C	7: 107,209,837 (GRCm38)	G110R	probably damaging	Het
Rpusd3	T	C	6: 113,418,064 (GRCm38)	T85A	probably benign	Het
Rttn	A	G	18: 89,108,063 (GRCm38)	I1853M	possibly damaging	Het
Sacs	T	A	14: 61,205,957 (GRCm38)	C1817*	probably null	Het
Scrib	A	T	15: 76,065,252 (GRCm38)	V335E	probably benign	Het
Sdk2	A	G	11: 113,825,086 (GRCm38)	L1549P	possibly damaging	Het
Slc25a11	C	T	11: 70,646,098 (GRCm38)	G44S	probably damaging	Het
Slc6a3	A	G	13: 73,571,451 (GRCm38)	Y533C	possibly damaging	Het
Slc7a5	A	G	8: 121,896,535 (GRCm38)	V191A	probably damaging	Het
Son	A	G	16: 91,654,733 (GRCm38)	K123E	probably damaging	Het
Tcstv3	T	A	13: 120,317,589 (GRCm38)	M8K	probably benign	Het
Tie1	A	G	4: 118,482,351 (GRCm38)	V411A	probably damaging	Het
Upk3bl	C	A	5: 136,060,123 (GRCm38)	D1E	possibly damaging	Het
Vmn2r5	T	C	3: 64,504,076 (GRCm38)	D357G	probably damaging	Het
Vmn2r75	T	C	7: 86,164,239 (GRCm38)	N452D	probably damaging	Het
Wnk2	T	A	13: 49,072,578 (GRCm38)	N1043I	possibly damaging	Het
Zfp407	A	T	18: 84,561,091 (GRCm38)	H632Q	probably damaging	Het
Zfp423	T	C	8: 87,773,647 (GRCm38)	K1174R	probably benign	Het

Other mutations in Rif1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00465:Rif1	APN	2	52,121,007 (GRCm38)	missense	probably damaging	0.96
IGL00711:Rif1	APN	2	52,111,070 (GRCm38)	missense	probably benign	0.00
IGL00721:Rif1	APN	2	52,119,117 (GRCm38)	missense	probably damaging	1.00
IGL01085:Rif1	APN	2	52,085,140 (GRCm38)	missense	possibly damaging	0.71
IGL01093:Rif1	APN	2	52,095,948 (GRCm38)	missense	probably damaging	1.00
IGL01107:Rif1	APN	2	52,111,303 (GRCm38)	missense	probably benign	0.00
IGL01138:Rif1	APN	2	52,111,522 (GRCm38)	missense	probably damaging	1.00
IGL01844:Rif1	APN	2	52,112,543 (GRCm38)	missense	probably benign	0.07
IGL02441:Rif1	APN	2	52,105,515 (GRCm38)	missense	probably benign	0.00
IGL02448:Rif1	APN	2	52,116,696 (GRCm38)	missense	probably damaging	0.99
IGL02563:Rif1	APN	2	52,077,065 (GRCm38)	missense	probably damaging	1.00
IGL02704:Rif1	APN	2	52,093,576 (GRCm38)	missense	probably damaging	1.00
IGL02946:Rif1	APN	2	52,110,125 (GRCm38)	nonsense	probably null
IGL03060:Rif1	APN	2	52,112,137 (GRCm38)	missense	probably damaging	0.97
IGL03206:Rif1	APN	2	52,103,622 (GRCm38)	missense	probably damaging	1.00
IGL03263:Rif1	APN	2	52,090,261 (GRCm38)	missense	probably damaging	0.99
IGL03267:Rif1	APN	2	52,076,988 (GRCm38)	missense	possibly damaging	0.94
IGL03280:Rif1	APN	2	52,112,599 (GRCm38)	missense	probably benign	0.32
hifi	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
nietzsche	UTSW	2	52,077,020 (GRCm38)	missense	probably benign	0.08
PIT4305001:Rif1	UTSW	2	52,111,958 (GRCm38)	missense
R0017:Rif1	UTSW	2	52,116,674 (GRCm38)	missense	probably benign	0.18
R0017:Rif1	UTSW	2	52,116,674 (GRCm38)	missense	probably benign	0.18
R0060:Rif1	UTSW	2	52,111,117 (GRCm38)	missense	probably damaging	1.00
R0060:Rif1	UTSW	2	52,111,117 (GRCm38)	missense	probably damaging	1.00
R0104:Rif1	UTSW	2	52,110,092 (GRCm38)	missense	possibly damaging	0.77
R0268:Rif1	UTSW	2	52,090,286 (GRCm38)	critical splice donor site	probably null
R0276:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0278:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0288:Rif1	UTSW	2	52,110,013 (GRCm38)	missense	probably damaging	1.00
R0314:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0345:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0346:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0383:Rif1	UTSW	2	52,085,141 (GRCm38)	missense	probably damaging	0.96
R0384:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0387:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0388:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0456:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0477:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0505:Rif1	UTSW	2	52,110,737 (GRCm38)	missense	probably damaging	0.99
R0510:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0511:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0512:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0633:Rif1	UTSW	2	52,112,563 (GRCm38)	missense	probably benign	0.00
R0637:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0638:Rif1	UTSW	2	52,111,588 (GRCm38)	missense	probably benign	0.12
R0666:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0675:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0707:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0726:Rif1	UTSW	2	52,110,353 (GRCm38)	missense	possibly damaging	0.52
R0743:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0744:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0938:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0939:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0940:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0941:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0942:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R0943:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1006:Rif1	UTSW	2	52,085,029 (GRCm38)	missense	probably damaging	0.99
R1052:Rif1	UTSW	2	52,111,562 (GRCm38)	missense	probably benign	0.01
R1061:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1175:Rif1	UTSW	2	52,107,628 (GRCm38)	unclassified	probably benign
R1183:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1184:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1271:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1332:Rif1	UTSW	2	52,078,314 (GRCm38)	missense	probably benign	0.06
R1336:Rif1	UTSW	2	52,078,314 (GRCm38)	missense	probably benign	0.06
R1351:Rif1	UTSW	2	52,111,555 (GRCm38)	missense	possibly damaging	0.74
R1517:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1527:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1560:Rif1	UTSW	2	52,111,131 (GRCm38)	missense	probably damaging	1.00
R1563:Rif1	UTSW	2	52,073,223 (GRCm38)	missense	probably damaging	0.99
R1571:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1625:Rif1	UTSW	2	52,103,640 (GRCm38)	missense	probably benign	0.25
R1679:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1689:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1731:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1744:Rif1	UTSW	2	52,112,392 (GRCm38)	missense	possibly damaging	0.56
R1746:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1748:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R1831:Rif1	UTSW	2	52,078,495 (GRCm38)	nonsense	probably null
R1902:Rif1	UTSW	2	52,116,673 (GRCm38)	missense	possibly damaging	0.93
R1964:Rif1	UTSW	2	52,098,409 (GRCm38)	missense	probably benign	0.01
R1978:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2000:Rif1	UTSW	2	52,081,298 (GRCm38)	missense	probably damaging	0.99
R2030:Rif1	UTSW	2	52,092,346 (GRCm38)	missense	probably damaging	1.00
R2056:Rif1	UTSW	2	52,093,576 (GRCm38)	missense	probably damaging	1.00
R2106:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2109:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2125:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2126:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2145:Rif1	UTSW	2	52,111,400 (GRCm38)	missense	possibly damaging	0.63
R2152:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2153:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2213:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2327:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2512:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2513:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2516:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2520:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R2905:Rif1	UTSW	2	52,098,504 (GRCm38)	missense	probably damaging	0.99
R3005:Rif1	UTSW	2	52,082,764 (GRCm38)	missense	probably damaging	1.00
R3155:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R3156:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R3429:Rif1	UTSW	2	52,110,324 (GRCm38)	unclassified	probably benign
R3707:Rif1	UTSW	2	52,093,580 (GRCm38)	missense	probably damaging	1.00
R3907:Rif1	UTSW	2	52,112,545 (GRCm38)	missense	probably benign	0.03
R3978:Rif1	UTSW	2	52,116,747 (GRCm38)	critical splice donor site	probably null
R4023:Rif1	UTSW	2	52,121,087 (GRCm38)	missense	probably benign	0.01
R4052:Rif1	UTSW	2	52,098,471 (GRCm38)	nonsense	probably null
R4668:Rif1	UTSW	2	52,111,952 (GRCm38)	missense	probably benign	0.01
R4674:Rif1	UTSW	2	52,106,942 (GRCm38)	missense	probably null	1.00
R4715:Rif1	UTSW	2	52,073,139 (GRCm38)	utr 5 prime	probably benign
R4766:Rif1	UTSW	2	52,098,934 (GRCm38)	missense	probably damaging	1.00
R4783:Rif1	UTSW	2	52,112,747 (GRCm38)	missense	probably damaging	0.96
R4785:Rif1	UTSW	2	52,112,747 (GRCm38)	missense	probably damaging	0.96
R4869:Rif1	UTSW	2	52,093,611 (GRCm38)	intron	probably benign
R4911:Rif1	UTSW	2	52,110,518 (GRCm38)	missense	probably damaging	0.98
R4951:Rif1	UTSW	2	52,084,986 (GRCm38)	splice site	probably null
R5044:Rif1	UTSW	2	52,109,928 (GRCm38)	missense	probably damaging	0.99
R5088:Rif1	UTSW	2	52,092,295 (GRCm38)	missense	possibly damaging	0.91
R5151:Rif1	UTSW	2	52,120,309 (GRCm38)	missense	probably damaging	1.00
R5187:Rif1	UTSW	2	52,081,289 (GRCm38)	missense	probably damaging	1.00
R5222:Rif1	UTSW	2	52,077,020 (GRCm38)	missense	probably benign	0.08
R5436:Rif1	UTSW	2	52,120,971 (GRCm38)	intron	probably benign
R5476:Rif1	UTSW	2	52,089,595 (GRCm38)	missense	probably damaging	1.00
R5496:Rif1	UTSW	2	52,098,916 (GRCm38)	missense	probably damaging	1.00
R5641:Rif1	UTSW	2	52,121,158 (GRCm38)	missense	possibly damaging	0.80
R5883:Rif1	UTSW	2	52,105,639 (GRCm38)	critical splice donor site	probably null
R5987:Rif1	UTSW	2	52,095,844 (GRCm38)	missense	probably damaging	1.00
R5990:Rif1	UTSW	2	52,095,844 (GRCm38)	missense	probably damaging	1.00
R5992:Rif1	UTSW	2	52,095,844 (GRCm38)	missense	probably damaging	1.00
R6019:Rif1	UTSW	2	52,095,844 (GRCm38)	missense	probably damaging	1.00
R6020:Rif1	UTSW	2	52,095,844 (GRCm38)	missense	probably damaging	1.00
R6255:Rif1	UTSW	2	52,085,053 (GRCm38)	missense	probably damaging	1.00
R6342:Rif1	UTSW	2	52,119,156 (GRCm38)	missense	probably damaging	0.97
R6364:Rif1	UTSW	2	52,107,669 (GRCm38)	missense	probably damaging	0.97
R6747:Rif1	UTSW	2	52,078,263 (GRCm38)	splice site	probably null
R6928:Rif1	UTSW	2	52,095,961 (GRCm38)	missense	probably damaging	1.00
R6954:Rif1	UTSW	2	52,112,691 (GRCm38)	missense	probably benign	0.00
R7003:Rif1	UTSW	2	52,076,989 (GRCm38)	missense	probably benign	0.06
R7310:Rif1	UTSW	2	52,105,619 (GRCm38)	missense	probably benign	0.12
R7549:Rif1	UTSW	2	52,078,507 (GRCm38)	missense	possibly damaging	0.52
R7603:Rif1	UTSW	2	52,076,175 (GRCm38)	missense	probably damaging	1.00
R7673:Rif1	UTSW	2	52,088,654 (GRCm38)	missense	probably damaging	1.00
R7741:Rif1	UTSW	2	52,085,141 (GRCm38)	missense	probably damaging	0.96
R7777:Rif1	UTSW	2	52,116,356 (GRCm38)	missense	probably benign	0.00
R7910:Rif1	UTSW	2	52,078,387 (GRCm38)	nonsense	probably null
R7962:Rif1	UTSW	2	52,074,276 (GRCm38)	missense	probably damaging	1.00
R8264:Rif1	UTSW	2	52,090,278 (GRCm38)	missense	noncoding transcript
R8390:Rif1	UTSW	2	52,110,923 (GRCm38)	missense	probably damaging	1.00
R8479:Rif1	UTSW	2	52,112,551 (GRCm38)	missense	possibly damaging	0.52
R8490:Rif1	UTSW	2	52,110,999 (GRCm38)	missense	probably damaging	0.96
R8762:Rif1	UTSW	2	52,111,730 (GRCm38)	missense
R8785:Rif1	UTSW	2	52,110,481 (GRCm38)	missense	probably benign	0.06
R8890:Rif1	UTSW	2	52,098,863 (GRCm38)	missense	probably damaging	0.99
R9081:Rif1	UTSW	2	52,110,977 (GRCm38)	missense	probably damaging	0.99
R9225:Rif1	UTSW	2	52,111,850 (GRCm38)	missense	probably benign	0.22
R9284:Rif1	UTSW	2	52,108,552 (GRCm38)	missense	probably benign	0.00
R9300:Rif1	UTSW	2	52,111,139 (GRCm38)	missense	probably damaging	1.00
R9366:Rif1	UTSW	2	52,120,344 (GRCm38)	missense
R9477:Rif1	UTSW	2	52,111,330 (GRCm38)	missense	probably benign	0.02
R9522:Rif1	UTSW	2	52,081,299 (GRCm38)	missense	probably damaging	1.00
R9573:Rif1	UTSW	2	52,110,454 (GRCm38)	missense	probably benign	0.29
R9630:Rif1	UTSW	2	52,089,595 (GRCm38)	missense	probably damaging	1.00
X0064:Rif1	UTSW	2	52,094,633 (GRCm38)	missense	probably damaging	0.96
X0064:Rif1	UTSW	2	52,074,315 (GRCm38)	missense	probably benign	0.00
Z1177:Rif1	UTSW	2	52,088,648 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AATTCACCCAAGTTGAATGCC -3'
(R):5'- GGCCCTTGGATTTCAGACAC -3'

Sequencing Primer
(F):5'- TTGAATGCCAACACAAGAGAAGTAG -3'
(R):5'- GGATTTCAGACACTGCTTCACTAG -3'

Posted On

2016-07-06