Mutagenetix > Incidental Mutation

Incidental Mutation 'R5259:Or7e166'

401307

Institutional Source

Beutler Lab

Gene Symbol

Or7e166

Ensembl Gene

ENSMUSG00000094678

Gene Name

olfactory receptor family 7 subfamily E member 166

Synonyms

MOR146-8P, Olfr857, GA_x6K02T2PVTD-13452606-13453535

MMRRC Submission

042856-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.070) question?

Stock #

R5259 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

19624125-19625054 bp(+) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

A to G at 19624109 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000150889 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000077023] [ENSMUST00000212013] [ENSMUST00000212013] [ENSMUST00000217450]

AlphaFold

A0A1L1SUS1

Predicted Effect

probably null
Transcript: ENSMUST00000077023

SMART Domains

Protein: ENSMUSP00000076281
Gene: ENSMUSG00000094678

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	2.5e-49	PFAM
Pfam:7TM_GPCR_Srsx	35	304	1.5e-5	PFAM
Pfam:7tm_1	41	290	9.4e-21	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000212013

Predicted Effect

probably null
Transcript: ENSMUST00000212013

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000212753

Predicted Effect

probably null
Transcript: ENSMUST00000217450

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.0%
20x: 94.7%

Validation Efficiency

100% (68/68)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 61 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abhd18	A	C	3: 40,871,325 (GRCm39)	T50P	probably damaging	Het
Adam5	T	A	8: 25,300,850 (GRCm39)	L226F	possibly damaging	Het
Adi1	T	C	12: 28,725,544 (GRCm39)		probably benign	Het
Apc	T	C	18: 34,447,343 (GRCm39)	V1379A	probably benign	Het
Atp13a4	G	A	16: 29,275,428 (GRCm39)	T352M	probably damaging	Het
Baat	T	A	4: 49,490,070 (GRCm39)	N338I	probably benign	Het
Bdnf	A	G	2: 109,554,327 (GRCm39)	T234A	probably benign	Het
Catsperd	A	C	17: 56,967,235 (GRCm39)	T539P	possibly damaging	Het
Cd109	A	T	9: 78,617,434 (GRCm39)	T1311S	probably benign	Het
Ceacam18	T	C	7: 43,286,536 (GRCm39)		probably null	Het
Cfap96	T	C	8: 46,415,373 (GRCm39)	E211G	probably benign	Het
Cfhr4	C	T	1: 139,668,233 (GRCm39)	C300Y	probably damaging	Het
Chsy3	T	A	18: 59,543,318 (GRCm39)	S819T	probably damaging	Het
Col4a4	G	T	1: 82,431,614 (GRCm39)	R1557S	unknown	Het
Ddx18	A	G	1: 121,495,518 (GRCm39)		probably null	Het
Depdc5	C	T	5: 33,095,635 (GRCm39)	P824L	probably damaging	Het
Fam13c	C	G	10: 70,276,893 (GRCm39)	A17G	probably benign	Het
Fermt1	T	C	2: 132,748,685 (GRCm39)	Y646C	probably damaging	Het
Fra10ac1	A	G	19: 38,188,110 (GRCm39)	S229P	probably benign	Het
Gbp8	A	T	5: 105,198,845 (GRCm39)	H23Q	probably benign	Het
Gdf2	T	C	14: 33,666,788 (GRCm39)	V170A	probably benign	Het
Gm15723	T	C	10: 114,652,722 (GRCm39)		noncoding transcript	Het
Gm815	G	A	19: 26,863,806 (GRCm39)	V16I	unknown	Het
Ighv1-75	T	A	12: 115,797,797 (GRCm39)	K42*	probably null	Het
Isx	A	G	8: 75,619,473 (GRCm39)	T222A	probably benign	Het
Itgax	G	A	7: 127,747,450 (GRCm39)	D1018N	probably damaging	Het
Kcnc4	A	G	3: 107,355,401 (GRCm39)	F349S	probably damaging	Het
Lama3	A	C	18: 12,598,565 (GRCm39)	S991R	probably damaging	Het
Larp4b	C	T	13: 9,208,220 (GRCm39)	A398V	probably damaging	Het
Ltbp1	A	G	17: 75,670,357 (GRCm39)	N1466S	probably benign	Het
Metrn	A	T	17: 26,015,514 (GRCm39)	L67Q	probably damaging	Het
Morc1	G	A	16: 48,451,132 (GRCm39)	R937Q	probably benign	Het
Mta3	A	G	17: 84,112,003 (GRCm39)	Y577C	probably damaging	Het
Nalcn	A	G	14: 123,753,063 (GRCm39)	F308L	possibly damaging	Het
Nat8	A	T	6: 85,807,873 (GRCm39)	S87T	probably benign	Het
Oplah	A	T	15: 76,185,410 (GRCm39)		probably null	Het
Or2ak6	A	G	11: 58,592,778 (GRCm39)	N84D	probably benign	Het
Or2ak6	A	C	11: 58,592,779 (GRCm39)	N84T	possibly damaging	Het
Pcdh15	A	T	10: 74,232,204 (GRCm39)	I668L	possibly damaging	Het
Pecr	A	G	1: 72,316,444 (GRCm39)		probably null	Het
Plxna4	T	C	6: 32,493,956 (GRCm39)	E220G	possibly damaging	Het
Pnma8b	A	G	7: 16,679,199 (GRCm39)	K61R	unknown	Het
Prl8a6	C	T	13: 27,620,179 (GRCm39)	W81*	probably null	Het
Rab33b	C	T	3: 51,392,033 (GRCm39)		probably benign	Het
Rbm33	T	A	5: 28,557,772 (GRCm39)		probably null	Het
Relch	A	G	1: 105,649,101 (GRCm39)	S747G	probably benign	Het
Reln	C	T	5: 22,308,395 (GRCm39)	V325M	possibly damaging	Het
Rheb	A	C	5: 25,008,743 (GRCm39)	D158E	probably benign	Het
Rhebl1	T	A	15: 98,778,464 (GRCm39)		probably benign	Het
Rmdn2	T	A	17: 79,975,446 (GRCm39)	Y312N	probably damaging	Het
Scamp1	T	G	13: 94,368,594 (GRCm39)	N58T	probably benign	Het
Semp2l1	A	T	1: 32,584,598 (GRCm39)	C437*	probably null	Het
Slc35a1	C	T	4: 34,683,322 (GRCm39)	V53M	probably benign	Het
Slc35f3	A	T	8: 127,115,872 (GRCm39)	L266F	probably damaging	Het
Slc45a2	C	T	15: 11,027,871 (GRCm39)	T480I	probably damaging	Het
Ticrr	T	A	7: 79,344,471 (GRCm39)	S1445R	probably benign	Het
Ttc23l	T	C	15: 10,515,236 (GRCm39)	N381D	probably damaging	Het
Usp17ld	T	A	7: 102,899,781 (GRCm39)	K384*	probably null	Het
Vmn1r20	T	A	6: 57,409,050 (GRCm39)	Y125*	probably null	Het
Zfp738	G	T	13: 67,817,805 (GRCm39)	Q729K	probably benign	Het
Zfp770	T	A	2: 114,027,674 (GRCm39)	M132L	probably benign	Het

Other mutations in Or7e166

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01510:Or7e166	APN	9	19,624,575 (GRCm39)	missense	probably benign	0.00
IGL01919:Or7e166	APN	9	19,624,638 (GRCm39)	missense	probably benign	0.00
IGL02157:Or7e166	APN	9	19,624,585 (GRCm39)	missense	probably benign	0.07
IGL02550:Or7e166	APN	9	19,624,343 (GRCm39)	missense	possibly damaging	0.92
IGL03329:Or7e166	APN	9	19,624,597 (GRCm39)	missense	probably benign	0.16
IGL02799:Or7e166	UTSW	9	19,624,314 (GRCm39)	missense	probably damaging	0.99
R0356:Or7e166	UTSW	9	19,624,743 (GRCm39)	missense	probably damaging	1.00
R0927:Or7e166	UTSW	9	19,624,945 (GRCm39)	missense	probably benign	0.39
R1161:Or7e166	UTSW	9	19,624,476 (GRCm39)	missense	probably damaging	1.00
R1848:Or7e166	UTSW	9	19,624,386 (GRCm39)	missense	probably benign	0.01
R5191:Or7e166	UTSW	9	19,624,630 (GRCm39)	missense	probably damaging	0.98
R5216:Or7e166	UTSW	9	19,624,585 (GRCm39)	missense	probably benign	0.07
R5342:Or7e166	UTSW	9	19,624,333 (GRCm39)	missense	probably damaging	1.00
R5506:Or7e166	UTSW	9	19,624,570 (GRCm39)	missense	possibly damaging	0.61
R5526:Or7e166	UTSW	9	19,624,994 (GRCm39)	nonsense	probably null
R5594:Or7e166	UTSW	9	19,624,302 (GRCm39)	missense	probably damaging	0.99
R5928:Or7e166	UTSW	9	19,625,049 (GRCm39)	missense	probably benign	0.02
R6569:Or7e166	UTSW	9	19,624,638 (GRCm39)	missense	probably benign	0.00
R6858:Or7e166	UTSW	9	19,624,765 (GRCm39)	missense	probably damaging	0.98
R7077:Or7e166	UTSW	9	19,624,428 (GRCm39)	missense	probably benign
R7378:Or7e166	UTSW	9	19,624,183 (GRCm39)	missense	probably damaging	1.00
R7771:Or7e166	UTSW	9	19,624,767 (GRCm39)	missense	probably benign
R8038:Or7e166	UTSW	9	19,624,976 (GRCm39)	missense	possibly damaging	0.52
R8160:Or7e166	UTSW	9	19,624,085 (GRCm39)	intron	probably benign
R8223:Or7e166	UTSW	9	19,624,705 (GRCm39)	missense	probably benign
R8400:Or7e166	UTSW	9	19,624,389 (GRCm39)	missense	probably benign	0.45
R8780:Or7e166	UTSW	9	19,624,653 (GRCm39)	missense	possibly damaging	0.92
R8946:Or7e166	UTSW	9	19,624,885 (GRCm39)	missense	probably damaging	0.99
R9164:Or7e166	UTSW	9	19,624,954 (GRCm39)	missense	probably benign	0.25
R9475:Or7e166	UTSW	9	19,624,939 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- AAGCTCCTAACCCTAGAACCTTTTC -3'
(R):5'- AGCCCATGTCAGACAATGAAAG -3'

Sequencing Primer
(F):5'- AACCCTAGAACCTTTTCCTGAG -3'
(R):5'- CCATGTCAGACAATGAAAGGTTGTAG -3'

Posted On

2016-07-06