Mutagenetix > Incidental Mutation

Incidental Mutation 'R5260:Zfp329'

401372

Institutional Source

Beutler Lab

Gene Symbol

Zfp329

Ensembl Gene

ENSMUSG00000057894

Gene Name

zinc finger protein 329

Synonyms

MMRRC Submission

042829-MU

Accession Numbers

Is this an essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5260 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

12804977-12818858 bp(-) (GRCm38)

Type of Mutation

unclassified

DNA Base Change (assembly)

A to G at 12806526 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000147550 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000072222] [ENSMUST00000108546] [ENSMUST00000121215] [ENSMUST00000210650]

AlphaFold

Q6GQR8

Predicted Effect

probably benign
Transcript: ENSMUST00000072222

SMART Domains

Protein: ENSMUSP00000072079
Gene: ENSMUSG00000057894

Domain	Start	End	E-Value	Type
low complexity region	147	160	N/A	INTRINSIC
ZnF_C2H2	184	206	9.58e-3	SMART
ZnF_C2H2	212	234	1.12e-3	SMART
ZnF_C2H2	240	262	1.22e-4	SMART
ZnF_C2H2	268	290	1.95e-3	SMART
ZnF_C2H2	296	318	2.61e-4	SMART
ZnF_C2H2	324	346	5.14e-3	SMART
ZnF_C2H2	352	374	2.24e-3	SMART
ZnF_C2H2	380	402	5.21e-4	SMART
ZnF_C2H2	408	430	1.92e-2	SMART
ZnF_C2H2	436	458	5.21e-4	SMART
ZnF_C2H2	464	486	3.16e-3	SMART
ZnF_C2H2	492	514	2.2e-2	SMART

Predicted Effect

unknown
Transcript: ENSMUST00000108546
AA Change: F307L

SMART Domains

Protein: ENSMUSP00000104186
Gene: ENSMUSG00000057894
AA Change: F307L

Domain	Start	End	E-Value	Type
low complexity region	147	160	N/A	INTRINSIC
ZnF_C2H2	184	206	9.58e-3	SMART
ZnF_C2H2	212	234	1.12e-3	SMART
ZnF_C2H2	240	262	1.22e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000121215

SMART Domains

Protein: ENSMUSP00000113355
Gene: ENSMUSG00000057894

Domain	Start	End	E-Value	Type
low complexity region	147	160	N/A	INTRINSIC
ZnF_C2H2	184	206	9.58e-3	SMART
ZnF_C2H2	212	234	1.12e-3	SMART
ZnF_C2H2	240	262	1.22e-4	SMART
ZnF_C2H2	268	290	1.95e-3	SMART
ZnF_C2H2	296	318	2.61e-4	SMART
ZnF_C2H2	324	346	5.14e-3	SMART
ZnF_C2H2	352	374	2.24e-3	SMART
ZnF_C2H2	380	402	5.21e-4	SMART
ZnF_C2H2	408	430	1.92e-2	SMART
ZnF_C2H2	436	458	5.21e-4	SMART
ZnF_C2H2	464	486	3.16e-3	SMART
ZnF_C2H2	492	514	2.2e-2	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000129647

Predicted Effect

probably benign
Transcript: ENSMUST00000210650

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.1%
20x: 95.0%

Validation Efficiency

99% (77/78)

Allele List at MGI

Other mutations in this stock

Total: 71 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310007B03Rik	A	T	1: 93,159,978	V51E	probably damaging	Het
Abraxas2	A	T	7: 132,859,274	I14F	probably damaging	Het
Acin1	T	C	14: 54,642,822		probably benign	Het
Adamts9	C	A	6: 92,807,137	V1579L	probably benign	Het
Adra2a	T	A	19: 54,046,608	C132S	probably damaging	Het
Aif1	T	A	17: 35,171,941		probably null	Het
Atf5	A	T	7: 44,815,086	Y27*	probably null	Het
Atm	A	G	9: 53,506,611	S799P	probably damaging	Het
Bmp2k	T	G	5: 97,087,351		probably benign	Het
Chaf1a	C	T	17: 56,065,000	H723Y	probably damaging	Het
Clint1	T	C	11: 45,907,942	W493R	probably damaging	Het
Cyb561a3	T	A	19: 10,587,866	V198D	possibly damaging	Het
Cyp2j8	T	C	4: 96,501,064	E174G	possibly damaging	Het
Cyp4v3	C	T	8: 45,306,980	G512S	probably damaging	Het
Dnah8	T	A	17: 30,700,419	V1122D	probably benign	Het
Doc2b	C	A	11: 75,786,163	G128V	probably damaging	Het
Dysf	T	C	6: 84,150,034	V1378A	probably damaging	Het
Efcab5	A	T	11: 77,137,651	S421T	possibly damaging	Het
Efcab6	T	G	15: 83,945,123	D672A	probably benign	Het
Eif2ak3	C	A	6: 70,893,129	H933Q	probably damaging	Het
Erc1	A	T	6: 119,761,159	N574K	probably damaging	Het
Eri2	A	T	7: 119,787,846		probably benign	Het
Faxc	A	G	4: 21,948,744	Y152C	probably damaging	Het
Fbxl7	T	A	15: 26,543,499	Y354F	probably damaging	Het
Fras1	T	A	5: 96,735,187	I2526N	possibly damaging	Het
Gm1966	A	G	7: 106,599,204		noncoding transcript	Het
Gm5424	A	T	10: 62,071,595		noncoding transcript	Het
Gm6728	T	C	6: 136,486,703		noncoding transcript	Het
Golgb1	A	T	16: 36,913,141	S917C	probably benign	Het
Gtpbp3	T	C	8: 71,489,418		probably benign	Het
Gusb	A	T	5: 129,999,988	Y220*	probably null	Het
Hmcn1	A	G	1: 150,595,861	V4914A	possibly damaging	Het
Iars2	C	A	1: 185,323,734	C211F	probably damaging	Het
Kif5b	A	G	18: 6,211,058	L802P	probably damaging	Het
Kif5c	A	G	2: 49,735,590	E624G	probably damaging	Het
Kmt2d	G	T	15: 98,842,860		probably benign	Het
Krt82	C	A	15: 101,548,388	G186C	possibly damaging	Het
Lca5	G	A	9: 83,423,223	R177C	probably damaging	Het
Mier1	T	C	4: 103,162,710	S318P	probably benign	Het
Obscn	A	T	11: 59,003,369	I1207N	probably damaging	Het
Olfr1161	A	C	2: 88,025,474	I251L	probably benign	Het
Olfr1261	A	G	2: 89,994,182	D263G	probably damaging	Het
Olfr1302	A	G	2: 111,781,181	Y287C	probably damaging	Het
Olfr607	A	G	7: 103,460,615	F198L	probably benign	Het
Oog4	C	T	4: 143,437,854	G369D	probably benign	Het
Plec	T	C	15: 76,176,624	T3060A	probably damaging	Het
Plekhh2	C	T	17: 84,577,165	T769I	probably damaging	Het
Prkaa1	A	T	15: 5,160,668	S65C	probably damaging	Het
Psma3	T	C	12: 70,984,642		probably benign	Het
Ptpn18	T	A	1: 34,463,510		probably benign	Het
Ptprv	T	C	1: 135,112,260		noncoding transcript	Het
Rac1	C	A	5: 143,508,131	V104L	probably benign	Het
Serpinb7	A	T	1: 107,434,749	N61I	possibly damaging	Het
Sirt7	A	C	11: 120,620,521		probably benign	Het
Srl	G	A	16: 4,482,895	R333*	probably null	Het
Srprb	A	T	9: 103,201,920	L756Q	probably damaging	Het
Tchhl1	T	C	3: 93,470,795	S269P	probably damaging	Het
Tdo2	C	T	3: 81,975,323		probably null	Het
Teddm1a	T	C	1: 153,891,900	Y37H	probably benign	Het
Tenm3	T	C	8: 48,236,855	Y1899C	probably damaging	Het
Tep1	T	C	14: 50,838,631	T1681A	probably benign	Het
Timm44	G	T	8: 4,275,919		probably null	Het
Trp73	A	G	4: 154,062,602	V322A	possibly damaging	Het
Tsr1	A	C	11: 74,905,955	E611A	probably damaging	Het
Unc80	A	G	1: 66,646,587	N2290S	possibly damaging	Het
Ush2a	T	C	1: 188,947,079	V4828A	possibly damaging	Het
Vps51	T	G	19: 6,071,033	E283D	probably benign	Het
Wnt9b	C	A	11: 103,732,049	S176I	possibly damaging	Het
Zfp352	T	A	4: 90,224,460	V279D	probably damaging	Het
Zfp932	C	T	5: 110,009,635	Q400*	probably null	Het
Zxdc	T	A	6: 90,382,093	L569Q	probably damaging	Het

Other mutations in Zfp329

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02501:Zfp329	APN	7	12811179	missense	possibly damaging	0.87
IGL02830:Zfp329	APN	7	12810116	missense	probably damaging	1.00
R0069:Zfp329	UTSW	7	12810932	missense	probably damaging	0.98
R0069:Zfp329	UTSW	7	12810932	missense	probably damaging	0.98
R0122:Zfp329	UTSW	7	12810987	missense	probably damaging	1.00
R0238:Zfp329	UTSW	7	12810829	missense	probably damaging	1.00
R0238:Zfp329	UTSW	7	12810829	missense	probably damaging	1.00
R0539:Zfp329	UTSW	7	12806593	critical splice acceptor site	probably null
R0570:Zfp329	UTSW	7	12810452	missense	probably damaging	1.00
R0682:Zfp329	UTSW	7	12810284	missense	probably damaging	1.00
R0811:Zfp329	UTSW	7	12811468	missense	probably benign
R0812:Zfp329	UTSW	7	12811468	missense	probably benign
R0944:Zfp329	UTSW	7	12811468	missense	probably benign
R0945:Zfp329	UTSW	7	12811468	missense	probably benign
R0946:Zfp329	UTSW	7	12811468	missense	probably benign
R0948:Zfp329	UTSW	7	12811468	missense	probably benign
R1632:Zfp329	UTSW	7	12810949	missense	possibly damaging	0.63
R1980:Zfp329	UTSW	7	12811468	missense	probably benign
R2172:Zfp329	UTSW	7	12810767	missense	probably damaging	1.00
R2897:Zfp329	UTSW	7	12810486	missense	probably damaging	1.00
R4256:Zfp329	UTSW	7	12807913	missense	probably benign	0.03
R4383:Zfp329	UTSW	7	12811657	start gained	probably benign
R4384:Zfp329	UTSW	7	12811657	start gained	probably benign
R4692:Zfp329	UTSW	7	12810632	missense	probably damaging	1.00
R5327:Zfp329	UTSW	7	12811494	missense	probably benign	0.04
R5679:Zfp329	UTSW	7	12810031	missense	probably damaging	0.96
R6886:Zfp329	UTSW	7	12810098	missense	probably benign	0.00
R6904:Zfp329	UTSW	7	12806530	unclassified	probably benign
R7304:Zfp329	UTSW	7	12810899	missense	probably damaging	1.00
R7564:Zfp329	UTSW	7	12811040	missense	probably damaging	1.00
R8130:Zfp329	UTSW	7	12810386	missense	probably damaging	1.00
R8310:Zfp329	UTSW	7	12810189	nonsense	probably null
R8788:Zfp329	UTSW	7	12810563	missense	possibly damaging	0.85
R9206:Zfp329	UTSW	7	12811158	missense	probably benign
R9497:Zfp329	UTSW	7	12810288	nonsense	probably null
R9656:Zfp329	UTSW	7	12810490	missense	probably damaging	1.00
R9707:Zfp329	UTSW	7	12810202	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- CACACATGTAATTGCCTTTCATTGG -3'
(R):5'- CCCAAGAATGTGTCAGTGCC -3'

Sequencing Primer
(F):5'- TTCATTGGGAAAGGAAATGAATGATC -3'
(R):5'- GAGCAGATTTTACCTCCAACATGG -3'

Posted On

2016-07-06