|Institutional Source||Beutler Lab|
|Gene Name||wingless-type MMTV integration site family, member 9B|
|Synonyms||Wnt15, Wnt14b, clf, clf1|
|Is this an essential gene?||Essential (E-score: 1.000)|
|Stock #||R5260 (G1)|
|Chromosomal Location||103727364-103749821 bp(-) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||C to A at 103732049 bp|
|Amino Acid Change||Serine to Isoleucine at position 176 (S176I)|
|Ref Sequence||ENSEMBL: ENSMUSP00000018630 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000018630]|
|Predicted Effect||possibly damaging
AA Change: S176I
PolyPhen 2 Score 0.867 (Sensitivity: 0.83; Specificity: 0.93)
AA Change: S176I
|Meta Mutation Damage Score||0.1938|
|Coding Region Coverage||
|Validation Efficiency||99% (77/78)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. Study of its expression in the teratocarcinoma cell line NT2 suggests that it may be implicated in the early process of neuronal differentiation of NT2 cells induced by retinoic acid. This gene is clustered with WNT3, another family member, in the chromosome 17q21 region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
PHENOTYPE: Homozygous null mice display neonatal lethality, disrupted ureteric bud branching, impaired Mullerian duct formation, and incompletely penetrant cleft lip and palate. In mice with alleles that decrease expression kidneys are smaller with fewer mature nephrons. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Wnt9b||
(F):5'- TACGCAGAGAAGGTTCACAAC -3'
(R):5'- GCACAGGCTTTAAGGAGACG -3'
(F):5'- TCACAACGGGGTCTGAAGTTCATC -3'
(R):5'- CTTTAAGGAGACGGCCTTCCTG -3'