Mutagenetix > Incidental Mutation

Incidental Mutation 'R5261:Zfp35'

401470

Institutional Source

Beutler Lab

Gene Symbol

Zfp35

Ensembl Gene

ENSMUSG00000063281

Gene Name

zinc finger protein 35

Synonyms

Zfp-35

MMRRC Submission

042830-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.335) question?

Stock #

R5261 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

24122689-24138433 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 24136778 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Arginine at position 374 (H374R)

Ref Sequence

ENSEMBL: ENSMUSP00000074475 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000074941]

AlphaFold

P15620

Predicted Effect

probably damaging
Transcript: ENSMUST00000074941
AA Change: H374R

PolyPhen 2 Score 0.977 (Sensitivity: 0.76; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000074475
Gene: ENSMUSG00000063281
AA Change: H374R

Domain	Start	End	E-Value	Type
ZnF_C2H2	78	100	1.28e-3	SMART
ZnF_C2H2	104	126	8.47e-4	SMART
ZnF_C2H2	132	154	5.21e-4	SMART
ZnF_C2H2	160	182	1.84e-4	SMART
ZnF_C2H2	188	210	7.37e-4	SMART
ZnF_C2H2	216	238	4.72e-2	SMART
ZnF_C2H2	244	266	2.4e-3	SMART
ZnF_C2H2	272	294	1.04e-3	SMART
ZnF_C2H2	300	322	3.69e-4	SMART
ZnF_C2H2	328	350	5.21e-4	SMART
ZnF_C2H2	356	378	1.5e-4	SMART
ZnF_C2H2	384	406	1.6e-4	SMART
ZnF_C2H2	412	434	3.89e-3	SMART
ZnF_C2H2	440	462	1.72e-4	SMART
ZnF_C2H2	468	490	2.71e-2	SMART
ZnF_C2H2	496	518	1.6e-4	SMART
ZnF_C2H2	524	545	2.17e1	SMART
ZnF_C2H2	551	573	1.6e-4	SMART

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 96.5%
20x: 93.1%

Validation Efficiency

96% (64/67)

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out mutation exhibit increased airway responsiveness to ovalbumin and methacholine. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
6820408C15Rik	C	G	2: 152,282,777 (GRCm39)	P211A	probably damaging	Het
Ambra1	G	A	2: 91,715,951 (GRCm39)	V761M	probably damaging	Het
Amfr	T	A	8: 94,702,798 (GRCm39)		probably null	Het
Asphd1	C	A	7: 126,545,287 (GRCm39)	A357S	probably benign	Het
Brd3	C	A	2: 27,353,931 (GRCm39)	Q60H	probably damaging	Het
Cd96	T	A	16: 45,890,016 (GRCm39)	M336L	probably benign	Het
Cep152	T	A	2: 125,406,125 (GRCm39)	H1469L	probably benign	Het
Coro1a	G	T	7: 126,299,816 (GRCm39)		probably null	Het
D630045J12Rik	A	T	6: 38,171,555 (GRCm39)	L871Q	probably benign	Het
Depdc5	C	T	5: 33,095,635 (GRCm39)	P824L	probably damaging	Het
Enpep	T	G	3: 129,099,075 (GRCm39)	D467A	probably damaging	Het
Extl2	G	A	3: 115,821,013 (GRCm39)	A273T	probably benign	Het
Foxi3	T	A	6: 70,937,500 (GRCm39)	F218Y	probably damaging	Het
Golga2	A	G	2: 32,194,166 (GRCm39)	M521V	probably benign	Het
Gpr142	T	A	11: 114,695,168 (GRCm39)	N44K	probably damaging	Het
Gtf2ird2	A	G	5: 134,245,061 (GRCm39)	I440V	probably benign	Het
Gucy1b2	T	A	14: 62,642,028 (GRCm39)	K698I	probably damaging	Het
Hspa1b	T	C	17: 35,177,983 (GRCm39)	M1V	probably null	Het
Ints9	T	A	14: 65,245,521 (GRCm39)	Y260N	probably benign	Het
Khdc3	T	G	9: 73,010,768 (GRCm39)	V182G	possibly damaging	Het
Ky	A	G	9: 102,414,798 (GRCm39)		probably null	Het
Map2k1	T	A	9: 64,098,843 (GRCm39)	I263F	probably damaging	Het
Or13a18	A	C	7: 140,190,576 (GRCm39)	I166L	probably benign	Het
Or2t47	T	C	11: 58,442,877 (GRCm39)	S63G	probably benign	Het
Or5ak25	A	G	2: 85,269,241 (GRCm39)	V87A	probably benign	Het
Otogl	T	A	10: 107,613,453 (GRCm39)	H2004L	probably benign	Het
Palmd	T	C	3: 116,717,009 (GRCm39)	H496R	probably benign	Het
Papolb	C	G	5: 142,515,409 (GRCm39)	R78P	possibly damaging	Het
Pcdh10	G	A	3: 45,336,247 (GRCm39)	G854R	probably damaging	Het
Pdcd11	A	G	19: 47,101,976 (GRCm39)	I1054V	probably benign	Het
Pik3ap1	A	T	19: 41,364,545 (GRCm39)	L58Q	probably damaging	Het
Ppp2ca	A	G	11: 51,989,937 (GRCm39)	K21R	probably benign	Het
Prmt5	A	C	14: 54,745,373 (GRCm39)	I598S	probably damaging	Het
Pycr1	T	A	11: 120,532,050 (GRCm39)	I239F	probably damaging	Het
R3hdm2	C	T	10: 127,334,285 (GRCm39)	R896C	probably damaging	Het
Rev3l	C	T	10: 39,722,725 (GRCm39)	P699S	probably damaging	Het
Samd1	CGAGGAGGAGGAGGAGGAGGA	CGAGGAGGAGGAGGAGGA	8: 84,725,625 (GRCm39)		probably benign	Het
Sel1l	A	T	12: 91,791,658 (GRCm39)	M351K	possibly damaging	Het
Sesn2	A	G	4: 132,226,617 (GRCm39)	L159P	probably damaging	Het
Slc35f4	T	A	14: 49,540,946 (GRCm39)		probably benign	Het
Slc3a1	C	A	17: 85,359,403 (GRCm39)	N409K	probably damaging	Het
Slc45a2	C	T	15: 11,027,871 (GRCm39)	T480I	probably damaging	Het
Slco1c1	T	C	6: 141,492,502 (GRCm39)	F246S	probably damaging	Het
Socs2	T	G	10: 95,228,681 (GRCm39)	I190L	unknown	Het
Srsf1	T	A	11: 87,938,684 (GRCm39)	I7N	possibly damaging	Het
Stox1	T	A	10: 62,503,620 (GRCm39)	H145L	probably damaging	Het
Trap1	A	T	16: 3,874,286 (GRCm39)	I243N	probably damaging	Het
Trav7-3	T	C	14: 53,681,207 (GRCm39)	I83T	probably benign	Het
Trmt9b	T	C	8: 36,979,078 (GRCm39)	I227T	probably benign	Het
Tuft1	A	T	3: 94,546,712 (GRCm39)	I42K	possibly damaging	Het
Umps	A	G	16: 33,787,344 (GRCm39)	V3A	probably benign	Het
Vim	A	C	2: 13,579,643 (GRCm39)	E134A	probably null	Het
Vmn1r28	C	A	6: 58,242,524 (GRCm39)	H122Q	probably benign	Het
Vps51	T	G	19: 6,121,063 (GRCm39)	E283D	probably benign	Het
Vsig10l	T	A	7: 43,120,274 (GRCm39)	V760D	probably damaging	Het
Xrn1	A	G	9: 95,927,596 (GRCm39)	D1460G	probably benign	Het
Zfp282	A	G	6: 47,874,824 (GRCm39)	D343G	probably damaging	Het

Other mutations in Zfp35

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0012:Zfp35	UTSW	18	24,136,001 (GRCm39)	missense	probably benign	0.01
R1453:Zfp35	UTSW	18	24,136,557 (GRCm39)	nonsense	probably null
R1807:Zfp35	UTSW	18	24,136,986 (GRCm39)	missense	probably benign	0.28
R2317:Zfp35	UTSW	18	24,136,555 (GRCm39)	missense	probably damaging	1.00
R2863:Zfp35	UTSW	18	24,137,352 (GRCm39)	missense	probably damaging	0.99
R3080:Zfp35	UTSW	18	24,136,367 (GRCm39)	missense	probably damaging	0.99
R3756:Zfp35	UTSW	18	24,135,982 (GRCm39)	missense	possibly damaging	0.88
R4114:Zfp35	UTSW	18	24,135,766 (GRCm39)	missense	probably benign	0.08
R4222:Zfp35	UTSW	18	24,136,246 (GRCm39)	missense	possibly damaging	0.91
R4780:Zfp35	UTSW	18	24,136,326 (GRCm39)	missense	probably damaging	1.00
R5137:Zfp35	UTSW	18	24,137,194 (GRCm39)	nonsense	probably null
R5375:Zfp35	UTSW	18	24,135,973 (GRCm39)	missense	possibly damaging	0.83
R6001:Zfp35	UTSW	18	24,135,816 (GRCm39)	missense	probably benign
R6190:Zfp35	UTSW	18	24,137,118 (GRCm39)	missense	probably benign	0.00
R6774:Zfp35	UTSW	18	24,136,015 (GRCm39)	missense	possibly damaging	0.51
R6850:Zfp35	UTSW	18	24,135,839 (GRCm39)	missense	possibly damaging	0.76
R6979:Zfp35	UTSW	18	24,136,927 (GRCm39)	missense	probably benign	0.32
R7029:Zfp35	UTSW	18	24,136,583 (GRCm39)	missense	probably damaging	0.98
R7079:Zfp35	UTSW	18	24,136,357 (GRCm39)	missense	possibly damaging	0.89
R7858:Zfp35	UTSW	18	24,136,897 (GRCm39)	missense	probably damaging	1.00
R8257:Zfp35	UTSW	18	24,137,288 (GRCm39)	missense	possibly damaging	0.55
R9477:Zfp35	UTSW	18	24,136,188 (GRCm39)	missense	possibly damaging	0.86
R9642:Zfp35	UTSW	18	24,137,155 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- ATGTGGGAAGGCTTTTAACCAGAG -3'
(R):5'- CTTCTAGGGCACTGTGTACACG -3'

Sequencing Primer
(F):5'- GAAGGCTTTTAACCAGAGCTCAGTC -3'
(R):5'- GCACTGTGTACACGCATATG -3'

Posted On

2016-07-06