Mutagenetix > Incidental Mutation

Incidental Mutation 'R5263:Gm10563'

401548

Institutional Source

Beutler Lab

Gene Symbol

Gm10563

Ensembl Gene

ENSMUSG00000073682

Gene Name

predicted gene 10563

Synonyms

MMRRC Submission

042831-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.034) question?

Stock #

R5263 (G1)

Quality Score

155

Status

Not validated

Chromosome

Chromosomal Location

155698870-155729865 bp(-) (GRCm39)

Type of Mutation

frame shift

DNA Base Change (assembly)

CTTT to CTTTATTT at 155698940 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000111488 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000043829] [ENSMUST00000105608] [ENSMUST00000115821] [ENSMUST00000118607]

AlphaFold

F6WIE4

Predicted Effect

probably benign
Transcript: ENSMUST00000043829

SMART Domains

Protein: ENSMUSP00000041449
Gene: ENSMUSG00000042202

Domain	Start	End	E-Value	Type
low complexity region	10	21	N/A	INTRINSIC
low complexity region	50	66	N/A	INTRINSIC
Pfam:TPT	73	368	7.9e-93	PFAM
Pfam:UAA	74	371	1e-10	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000105608

SMART Domains

Protein: ENSMUSP00000101233
Gene: ENSMUSG00000042202

Domain	Start	End	E-Value	Type
low complexity region	10	21	N/A	INTRINSIC
low complexity region	50	66	N/A	INTRINSIC
Pfam:UAA	75	375	6.9e-10	PFAM
Pfam:EamA	84	215	5.8e-8	PFAM
Pfam:TPT	224	369	3.8e-34	PFAM
Pfam:EamA	237	369	3.4e-9	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000115821

SMART Domains

Protein: ENSMUSP00000111488
Gene: ENSMUSG00000073682

Domain	Start	End	E-Value	Type
SCOP:d1l9ha_	17	52	2e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000118607

SMART Domains

Protein: ENSMUSP00000113189
Gene: ENSMUSG00000042202

Domain	Start	End	E-Value	Type
low complexity region	10	21	N/A	INTRINSIC
low complexity region	50	66	N/A	INTRINSIC
Pfam:UAA	75	375	6.9e-10	PFAM
Pfam:EamA	84	215	5.8e-8	PFAM
Pfam:TPT	224	369	3.8e-34	PFAM
Pfam:EamA	237	369	3.4e-9	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000128841

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000137752

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000151425

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000154931

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.9%
20x: 94.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 35 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adh4	G	T	3: 138,133,816 (GRCm39)	V309L	probably benign	Het
Agmo	T	C	12: 37,407,680 (GRCm39)	V188A	probably benign	Het
Aqr	A	G	2: 113,947,059 (GRCm39)	M1041T	probably damaging	Het
Arhgef4	T	A	1: 34,764,078 (GRCm39)	S1111R	possibly damaging	Het
Ascc3	A	C	10: 50,592,757 (GRCm39)	E1144D	probably benign	Het
Cct3	T	C	3: 88,228,672 (GRCm39)		probably null	Het
Cd209f	T	C	8: 4,154,506 (GRCm39)	T114A	probably benign	Het
Cgnl1	G	A	9: 71,539,936 (GRCm39)	Q1103*	probably null	Het
Cop1	G	A	1: 159,152,507 (GRCm39)	D586N	probably damaging	Het
Dcaf5	A	G	12: 80,395,120 (GRCm39)	S350P	probably damaging	Het
Dhx29	T	C	13: 113,084,755 (GRCm39)	C658R	probably damaging	Het
Dync1i1	G	A	6: 5,969,446 (GRCm39)	V424I	possibly damaging	Het
Gfap	C	T	11: 102,787,756 (GRCm39)	R63Q	probably damaging	Het
Gprc6a	C	A	10: 51,502,900 (GRCm39)	G321V	probably damaging	Het
Itgb4	C	T	11: 115,874,983 (GRCm39)	R447W	probably benign	Het
Izumo1r	A	G	9: 14,812,976 (GRCm39)	C99R	probably damaging	Het
Lrp1b	T	C	2: 41,850,691 (GRCm39)	D102G	probably damaging	Het
Mettl4	G	A	17: 95,047,937 (GRCm39)	Q235*	probably null	Het
Mmrn2	A	G	14: 34,121,541 (GRCm39)	T804A	probably benign	Het
Mrgprb5	A	G	7: 47,817,937 (GRCm39)	V266A	probably damaging	Het
Ntng1	T	C	3: 109,842,188 (GRCm39)	D195G	probably damaging	Het
Pld4	T	C	12: 112,731,465 (GRCm39)	L206P	probably damaging	Het
Polr3a	T	C	14: 24,505,009 (GRCm39)	I1084V	possibly damaging	Het
Prkd1	A	G	12: 50,435,089 (GRCm39)	L546P	probably damaging	Het
Rhob	A	T	12: 8,549,232 (GRCm39)	M134K	probably benign	Het
Ryr3	A	G	2: 112,548,347 (GRCm39)	S3087P	possibly damaging	Het
Sema6c	T	C	3: 95,080,463 (GRCm39)	L887P	probably benign	Het
Sltm	T	C	9: 70,492,081 (GRCm39)	S648P	unknown	Het
Sucnr1	A	G	3: 59,994,190 (GRCm39)	I239M	possibly damaging	Het
Tgtp2	T	A	11: 48,950,090 (GRCm39)	M161L	probably damaging	Het
Trpm7	A	G	2: 126,663,137 (GRCm39)	V1037A	probably benign	Het
Vmn2r13	A	T	5: 109,321,841 (GRCm39)	H285Q	probably benign	Het
Zfp971	T	A	2: 177,675,555 (GRCm39)	C385S	probably damaging	Het
Zfpm2	T	A	15: 40,962,791 (GRCm39)	V283E	probably benign	Het
Zranb1	CTGATGATGATG	CTGATGATGATGATG	7: 132,584,556 (GRCm39)		probably benign	Het

Other mutations in Gm10563

Allele	Source	Chr	Coord	Type	Predicted Effect
R1762:Gm10563	UTSW	4	155,720,337 (GRCm39)	intron	probably benign
R1784:Gm10563	UTSW	4	155,720,337 (GRCm39)	intron	probably benign
R1785:Gm10563	UTSW	4	155,720,337 (GRCm39)	intron	probably benign
R5265:Gm10563	UTSW	4	155,698,953 (GRCm39)	frame shift	probably null
R5291:Gm10563	UTSW	4	155,698,969 (GRCm39)	intron	probably benign
R9205:Gm10563	UTSW	4	155,720,307 (GRCm39)	small deletion	probably benign

Predicted Primers

PCR Primer
(F):5'- ACTGCCCTGTTGAGTAAGCC -3'
(R):5'- AGATCAGATACGCCACAGTG -3'

Sequencing Primer
(F):5'- GCCCTGTTGAGTAAGCCTCTAAAAG -3'
(R):5'- GTGAAACCACACAAGTAGCCAATAGG -3'

Posted On

2016-07-06