Mutagenetix > Incidental Mutation

Incidental Mutation 'R5263:Vmn2r13'

401549

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r13

Ensembl Gene

ENSMUSG00000091635

Gene Name

vomeronasal 2, receptor 13

Synonyms

Gm4867

MMRRC Submission

042831-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.086) question?

Stock #

R5263 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

109156068-109192107 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 109173975 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Glutamine at position 285 (H285Q)

Ref Sequence

ENSEMBL: ENSMUSP00000052977 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000053253]

AlphaFold

L7N1X2

Predicted Effect

probably benign
Transcript: ENSMUST00000053253
AA Change: H285Q

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000052977
Gene: ENSMUSG00000091635
AA Change: H285Q

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
Pfam:ANF_receptor	76	463	2.8e-29	PFAM
Pfam:NCD3G	506	560	1.3e-18	PFAM
Pfam:7tm_3	593	828	1.8e-54	PFAM

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.9%
20x: 94.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 35 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adh4	G	T	3: 138,428,055	V309L	probably benign	Het
Agmo	T	C	12: 37,357,681	V188A	probably benign	Het
Aqr	A	G	2: 114,116,578	M1041T	probably damaging	Het
Arhgef4	T	A	1: 34,724,997	S1111R	possibly damaging	Het
Ascc3	A	C	10: 50,716,661	E1144D	probably benign	Het
Cct3	T	C	3: 88,321,365		probably null	Het
Cd209f	T	C	8: 4,104,506	T114A	probably benign	Het
Cgnl1	G	A	9: 71,632,654	Q1103*	probably null	Het
Cop1	G	A	1: 159,324,937	D586N	probably damaging	Het
Dcaf5	A	G	12: 80,348,346	S350P	probably damaging	Het
Dhx29	T	C	13: 112,948,221	C658R	probably damaging	Het
Dync1i1	G	A	6: 5,969,446	V424I	possibly damaging	Het
Gfap	C	T	11: 102,896,930	R63Q	probably damaging	Het
Gm10563	CTTT	CTTTATTT	4: 155,614,483		probably null	Het
Gprc6a	C	A	10: 51,626,804	G321V	probably damaging	Het
Itgb4	C	T	11: 115,984,157	R447W	probably benign	Het
Izumo1r	A	G	9: 14,901,680	C99R	probably damaging	Het
Lrp1b	T	C	2: 41,960,679	D102G	probably damaging	Het
Mettl4	G	A	17: 94,740,509	Q235*	probably null	Het
Mmrn2	A	G	14: 34,399,584	T804A	probably benign	Het
Mrgprb5	A	G	7: 48,168,189	V266A	probably damaging	Het
Ntng1	T	C	3: 109,934,872	D195G	probably damaging	Het
Pld4	T	C	12: 112,765,031	L206P	probably damaging	Het
Polr3a	T	C	14: 24,454,941	I1084V	possibly damaging	Het
Prkd1	A	G	12: 50,388,306	L546P	probably damaging	Het
Rhob	A	T	12: 8,499,232	M134K	probably benign	Het
Ryr3	A	G	2: 112,718,002	S3087P	possibly damaging	Het
Sema6c	T	C	3: 95,173,152	L887P	probably benign	Het
Sltm	T	C	9: 70,584,799	S648P	unknown	Het
Sucnr1	A	G	3: 60,086,769	I239M	possibly damaging	Het
Tgtp2	T	A	11: 49,059,263	M161L	probably damaging	Het
Trpm7	A	G	2: 126,821,217	V1037A	probably benign	Het
Zfp971	T	A	2: 178,033,762	C385S	probably damaging	Het
Zfpm2	T	A	15: 41,099,395	V283E	probably benign	Het
Zranb1	CTGATGATGATG	CTGATGATGATGATG	7: 132,982,827		probably benign	Het

Other mutations in Vmn2r13

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00965:Vmn2r13	APN	5	109156098	missense	probably damaging	1.00
IGL01373:Vmn2r13	APN	5	109156702	missense	probably damaging	1.00
IGL01946:Vmn2r13	APN	5	109174219	missense	probably benign	0.01
IGL01971:Vmn2r13	APN	5	109174115	missense	probably benign	0.01
IGL02636:Vmn2r13	APN	5	109192017	missense	probably damaging	0.98
IGL03062:Vmn2r13	APN	5	109156282	missense	probably damaging	1.00
IGL03173:Vmn2r13	APN	5	109171779	missense	possibly damaging	0.95
IGL03301:Vmn2r13	APN	5	109158089	missense	probably damaging	0.99
IGL03383:Vmn2r13	APN	5	109156532	missense	probably damaging	0.98
IGL03048:Vmn2r13	UTSW	5	109156285	missense	probably damaging	1.00
R0123:Vmn2r13	UTSW	5	109175049	missense	probably benign	0.00
R0134:Vmn2r13	UTSW	5	109175049	missense	probably benign	0.00
R0220:Vmn2r13	UTSW	5	109156466	missense	probably damaging	1.00
R0225:Vmn2r13	UTSW	5	109175049	missense	probably benign	0.00
R0393:Vmn2r13	UTSW	5	109156529	missense	probably benign	0.01
R0410:Vmn2r13	UTSW	5	109173813	missense	probably benign	0.35
R0787:Vmn2r13	UTSW	5	109156847	missense	probably damaging	0.99
R1200:Vmn2r13	UTSW	5	109174202	missense	probably damaging	1.00
R1448:Vmn2r13	UTSW	5	109174135	missense	probably damaging	1.00
R1782:Vmn2r13	UTSW	5	109158174	missense	probably benign	0.08
R1939:Vmn2r13	UTSW	5	109191986	missense	possibly damaging	0.88
R2029:Vmn2r13	UTSW	5	109192077	missense	probably benign	0.13
R2125:Vmn2r13	UTSW	5	109158192	missense	probably benign	0.00
R2126:Vmn2r13	UTSW	5	109158192	missense	probably benign	0.00
R2379:Vmn2r13	UTSW	5	109171778	missense	probably benign	0.05
R2680:Vmn2r13	UTSW	5	109174312	missense	possibly damaging	0.66
R2888:Vmn2r13	UTSW	5	109191974	missense	possibly damaging	0.88
R2889:Vmn2r13	UTSW	5	109191974	missense	possibly damaging	0.88
R2890:Vmn2r13	UTSW	5	109191974	missense	possibly damaging	0.88
R3014:Vmn2r13	UTSW	5	109171761	missense	possibly damaging	0.81
R3683:Vmn2r13	UTSW	5	109156855	missense	probably damaging	1.00
R4074:Vmn2r13	UTSW	5	109156700	missense	probably damaging	1.00
R4599:Vmn2r13	UTSW	5	109156456	missense	probably damaging	1.00
R4614:Vmn2r13	UTSW	5	109175199	missense	probably benign	0.01
R4805:Vmn2r13	UTSW	5	109156465	missense	probably damaging	1.00
R4822:Vmn2r13	UTSW	5	109174072	missense	probably damaging	0.99
R4943:Vmn2r13	UTSW	5	109175049	missense	probably benign	0.00
R5297:Vmn2r13	UTSW	5	109191939	missense	probably benign	0.00
R5502:Vmn2r13	UTSW	5	109173714	missense	probably damaging	1.00
R5554:Vmn2r13	UTSW	5	109191994	missense	possibly damaging	0.49
R5563:Vmn2r13	UTSW	5	109173980	missense	probably benign	0.00
R5819:Vmn2r13	UTSW	5	109174100	missense	possibly damaging	0.79
R6074:Vmn2r13	UTSW	5	109174301	missense	probably benign	0.04
R6416:Vmn2r13	UTSW	5	109174116	missense	probably damaging	0.99
R6419:Vmn2r13	UTSW	5	109175219	missense	possibly damaging	0.87
R6484:Vmn2r13	UTSW	5	109156674	nonsense	probably null
R6486:Vmn2r13	UTSW	5	109156559	missense	probably benign	0.05
R6545:Vmn2r13	UTSW	5	109156940	splice site	probably null
R6700:Vmn2r13	UTSW	5	109175072	missense	probably benign	0.00
R6897:Vmn2r13	UTSW	5	109158149	missense	possibly damaging	0.90
R6957:Vmn2r13	UTSW	5	109156887	nonsense	probably null
R7276:Vmn2r13	UTSW	5	109173779	missense	probably damaging	1.00
R7363:Vmn2r13	UTSW	5	109192043	missense	probably benign	0.03
R7443:Vmn2r13	UTSW	5	109192043	missense	probably benign	0.03
R7555:Vmn2r13	UTSW	5	109171691	splice site	probably null
R7607:Vmn2r13	UTSW	5	109173640	missense	probably damaging	0.98
R7719:Vmn2r13	UTSW	5	109171752	missense	probably benign	0.00
R8116:Vmn2r13	UTSW	5	109175060	missense	probably benign	0.12
R8242:Vmn2r13	UTSW	5	109175006	missense	possibly damaging	0.65
R8294:Vmn2r13	UTSW	5	109175112	missense	probably benign	0.02
R8340:Vmn2r13	UTSW	5	109174140	missense	probably benign	0.00
R8692:Vmn2r13	UTSW	5	109171648	missense	probably benign	0.03
R8742:Vmn2r13	UTSW	5	109156397	missense	probably benign	0.02
R9022:Vmn2r13	UTSW	5	109156376	missense	possibly damaging	0.94
R9281:Vmn2r13	UTSW	5	109156087	missense	probably damaging	1.00
R9529:Vmn2r13	UTSW	5	109156198	missense	probably damaging	1.00
R9708:Vmn2r13	UTSW	5	109174141	missense	probably benign	0.00
R9746:Vmn2r13	UTSW	5	109191907	critical splice donor site	probably null
X0066:Vmn2r13	UTSW	5	109156219	missense	probably benign	0.44

Predicted Primers

PCR Primer
(F):5'- ACCCCAGTCTAGTCTCAGAAATG -3'
(R):5'- CTCAGATGATGACCAAGGTATTCAG -3'

Sequencing Primer
(F):5'- CCCAGTCTAGTCTCAGAAATGTTTAC -3'
(R):5'- GACCAAGGTATTCAGTTTCTATCAG -3'

Posted On

2016-07-06