Mutagenetix > Incidental Mutation

Incidental Mutation 'R5264:Tcp11l2'

401611

Institutional Source

Beutler Lab

Gene Symbol

Tcp11l2

Ensembl Gene

ENSMUSG00000020034

Gene Name

t-complex 11 (mouse) like 2

Synonyms

E430026E19Rik

MMRRC Submission

042832-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.111) question?

Stock #

R5264 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

84412811-84450219 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 84449524 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Methionine at position 496 (I496M)

Ref Sequence

ENSEMBL: ENSMUSP00000020223 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000020223]

AlphaFold

Q8K1H7

Predicted Effect

probably damaging
Transcript: ENSMUST00000020223
AA Change: I496M

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000020223
Gene: ENSMUSG00000020034
AA Change: I496M

Domain	Start	End	E-Value	Type
low complexity region	11	21	N/A	INTRINSIC
low complexity region	36	55	N/A	INTRINSIC
Pfam:Tcp11	77	497	5.8e-103	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000158471

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000181277

Meta Mutation Damage Score

0.6389

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.0%
20x: 94.8%

Validation Efficiency

98% (54/55)

Allele List at MGI

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ckap2l	A	T	2: 129,127,299 (GRCm39)	M293K	probably benign	Het
Col4a4	C	T	1: 82,471,312 (GRCm39)	G681E	unknown	Het
Dnai7	A	G	6: 145,127,502 (GRCm39)	V469A	probably benign	Het
Efcab7	G	A	4: 99,735,372 (GRCm39)	R132H	probably benign	Het
Elovl4	T	C	9: 83,662,817 (GRCm39)	T239A	probably benign	Het
Fank1	A	G	7: 133,481,621 (GRCm39)	D240G	probably damaging	Het
Fbln5	T	A	12: 101,723,703 (GRCm39)	M346L	possibly damaging	Het
Fbxl21	T	C	13: 56,680,136 (GRCm39)	F174L	probably benign	Het
Gns	A	G	10: 121,216,090 (GRCm39)	D279G	probably benign	Het
Hmcn1	A	G	1: 150,555,265 (GRCm39)	V2502A	probably benign	Het
Itgb4	C	T	11: 115,874,983 (GRCm39)	R447W	probably benign	Het
Large2	A	G	2: 92,205,088 (GRCm39)		probably benign	Het
Lrrc8b	A	T	5: 105,628,118 (GRCm39)	I155F	probably damaging	Het
Morc2b	T	C	17: 33,357,353 (GRCm39)	I140V	probably benign	Het
Mrgprb5	G	A	7: 47,817,796 (GRCm39)	S313L	probably benign	Het
Nectin4	A	T	1: 171,211,273 (GRCm39)	T266S	probably benign	Het
Nsd1	C	T	13: 55,395,159 (GRCm39)	A1023V	possibly damaging	Het
Or51a39	T	C	7: 102,363,558 (GRCm39)	T21A	probably benign	Het
Paqr8	A	G	1: 21,005,332 (GRCm39)	H162R	possibly damaging	Het
Pclo	G	A	5: 14,726,937 (GRCm39)		probably benign	Het
Phactr4	T	C	4: 132,098,293 (GRCm39)	D325G	probably damaging	Het
Plcg2	A	T	8: 118,361,532 (GRCm39)	E1255V	possibly damaging	Het
Polr3a	T	C	14: 24,505,009 (GRCm39)	I1084V	possibly damaging	Het
Polr3b	A	T	10: 84,503,280 (GRCm39)	Q399L	probably benign	Het
Ppp1r35	G	A	5: 137,778,286 (GRCm39)		probably benign	Het
Psd3	A	T	8: 68,166,377 (GRCm39)	D919E	probably benign	Het
Ptgs2	A	G	1: 149,978,481 (GRCm39)	T198A	possibly damaging	Het
Ptpn14	T	C	1: 189,564,997 (GRCm39)		probably null	Het
Ptprk	A	G	10: 28,461,582 (GRCm39)	Y39C	probably damaging	Het
R3hdm4	A	G	10: 79,749,175 (GRCm39)	Y75H	probably benign	Het
Rsph4a	A	G	10: 33,785,379 (GRCm39)	Y430C	probably damaging	Het
Samd12	C	T	15: 53,723,669 (GRCm39)	C8Y	probably damaging	Het
Sema3e	A	C	5: 14,276,662 (GRCm39)	L314F	probably damaging	Het
Sis	A	G	3: 72,857,089 (GRCm39)	F401L	probably damaging	Het
Smoc1	T	A	12: 81,151,474 (GRCm39)	S64T	probably damaging	Het
Socs5	T	A	17: 87,441,769 (GRCm39)	H236Q	probably damaging	Het
Spaca1	C	A	4: 34,049,863 (GRCm39)	R45L	possibly damaging	Het
Spag6	A	G	2: 18,750,324 (GRCm39)	K457E	probably benign	Het
Stat2	T	A	10: 128,116,934 (GRCm39)		probably null	Het
Ttll4	A	G	1: 74,725,535 (GRCm39)	I648V	possibly damaging	Het
Vmn2r67	T	C	7: 84,801,453 (GRCm39)	Y161C	probably damaging	Het
Wnt5b	A	T	6: 119,410,813 (GRCm39)	V171E	probably damaging	Het
Zfp236	T	C	18: 82,648,219 (GRCm39)	K933E	probably damaging	Het
Zfp236	T	C	18: 82,676,198 (GRCm39)	E373G	probably damaging	Het
Zfp617	A	G	8: 72,686,885 (GRCm39)	Y405C	probably damaging	Het
Zranb1	CTGATGATGATG	CTGATGATGATGATG	7: 132,584,556 (GRCm39)		probably benign	Het

Other mutations in Tcp11l2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00725:Tcp11l2	APN	10	84,430,574 (GRCm39)	missense	possibly damaging	0.82
IGL00845:Tcp11l2	APN	10	84,440,847 (GRCm39)	missense	possibly damaging	0.95
IGL02375:Tcp11l2	APN	10	84,440,932 (GRCm39)	critical splice donor site	probably null
IGL02418:Tcp11l2	APN	10	84,449,470 (GRCm39)	nonsense	probably null
IGL03325:Tcp11l2	APN	10	84,440,764 (GRCm39)	missense	possibly damaging	0.76
R0031:Tcp11l2	UTSW	10	84,427,004 (GRCm39)	missense	probably damaging	0.98
R0591:Tcp11l2	UTSW	10	84,440,458 (GRCm39)	missense	probably benign	0.05
R1563:Tcp11l2	UTSW	10	84,420,808 (GRCm39)	missense	probably damaging	0.96
R1607:Tcp11l2	UTSW	10	84,449,351 (GRCm39)	missense	probably damaging	1.00
R1840:Tcp11l2	UTSW	10	84,440,463 (GRCm39)	missense	probably damaging	0.98
R2144:Tcp11l2	UTSW	10	84,449,363 (GRCm39)	missense	probably damaging	1.00
R2251:Tcp11l2	UTSW	10	84,440,933 (GRCm39)	critical splice donor site	probably null
R4289:Tcp11l2	UTSW	10	84,440,937 (GRCm39)	splice site	probably null
R4639:Tcp11l2	UTSW	10	84,420,800 (GRCm39)	missense	probably damaging	1.00
R4844:Tcp11l2	UTSW	10	84,449,555 (GRCm39)	missense	probably benign	0.00
R4973:Tcp11l2	UTSW	10	84,427,027 (GRCm39)	missense	probably damaging	0.98
R5970:Tcp11l2	UTSW	10	84,430,661 (GRCm39)	splice site	probably benign
R6966:Tcp11l2	UTSW	10	84,427,133 (GRCm39)	missense	possibly damaging	0.79
R7250:Tcp11l2	UTSW	10	84,423,105 (GRCm39)	critical splice donor site	probably null
R7535:Tcp11l2	UTSW	10	84,430,523 (GRCm39)	missense	possibly damaging	0.67
R7565:Tcp11l2	UTSW	10	84,422,998 (GRCm39)	missense	probably damaging	1.00
R7619:Tcp11l2	UTSW	10	84,430,622 (GRCm39)	missense	probably damaging	1.00
R7774:Tcp11l2	UTSW	10	84,440,847 (GRCm39)	missense	possibly damaging	0.95
R8145:Tcp11l2	UTSW	10	84,444,480 (GRCm39)	missense	probably damaging	1.00
R8379:Tcp11l2	UTSW	10	84,449,469 (GRCm39)	missense	probably damaging	1.00
R8458:Tcp11l2	UTSW	10	84,449,396 (GRCm39)	nonsense	probably null
R8821:Tcp11l2	UTSW	10	84,449,522 (GRCm39)	missense	probably damaging	1.00
R8831:Tcp11l2	UTSW	10	84,449,522 (GRCm39)	missense	probably damaging	1.00
RF008:Tcp11l2	UTSW	10	84,449,388 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- CGGATCCAGTTGTACATGAAAGGC -3'
(R):5'- TGCTTTAAACACAAGAGGGGTG -3'

Sequencing Primer
(F):5'- TGTACATGAAAGGCCTGCTC -3'
(R):5'- TCCATTACCAGGTTACACGGATG -3'

Posted On

2016-07-06