Mutagenetix > Incidental Mutation

Incidental Mutation 'R5265:Gm10563'

401638

Institutional Source

Beutler Lab

Gene Symbol

Gm10563

Ensembl Gene

ENSMUSG00000073682

Gene Name

predicted gene 10563

Synonyms

MMRRC Submission

042833-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.034) question?

Stock #

R5265 (G1)

Quality Score

111

Status

Validated

Chromosome

Chromosomal Location

155698870-155729865 bp(-) (GRCm39)

Type of Mutation

frame shift

DNA Base Change (assembly)

TTTC to TTTCATTC at 155698953 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000111488 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000043829] [ENSMUST00000105608] [ENSMUST00000115821] [ENSMUST00000118607]

AlphaFold

F6WIE4

Predicted Effect

probably benign
Transcript: ENSMUST00000043829

SMART Domains

Protein: ENSMUSP00000041449
Gene: ENSMUSG00000042202

Domain	Start	End	E-Value	Type
low complexity region	10	21	N/A	INTRINSIC
low complexity region	50	66	N/A	INTRINSIC
Pfam:TPT	73	368	7.9e-93	PFAM
Pfam:UAA	74	371	1e-10	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000105608

SMART Domains

Protein: ENSMUSP00000101233
Gene: ENSMUSG00000042202

Domain	Start	End	E-Value	Type
low complexity region	10	21	N/A	INTRINSIC
low complexity region	50	66	N/A	INTRINSIC
Pfam:UAA	75	375	6.9e-10	PFAM
Pfam:EamA	84	215	5.8e-8	PFAM
Pfam:TPT	224	369	3.8e-34	PFAM
Pfam:EamA	237	369	3.4e-9	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000115821

SMART Domains

Protein: ENSMUSP00000111488
Gene: ENSMUSG00000073682

Domain	Start	End	E-Value	Type
SCOP:d1l9ha_	17	52	2e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000118607

SMART Domains

Protein: ENSMUSP00000113189
Gene: ENSMUSG00000042202

Domain	Start	End	E-Value	Type
low complexity region	10	21	N/A	INTRINSIC
low complexity region	50	66	N/A	INTRINSIC
Pfam:UAA	75	375	6.9e-10	PFAM
Pfam:EamA	84	215	5.8e-8	PFAM
Pfam:TPT	224	369	3.8e-34	PFAM
Pfam:EamA	237	369	3.4e-9	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000128841

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000137752

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000151425

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000154931

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.0%
20x: 94.7%

Validation Efficiency

100% (59/59)

Allele List at MGI

Other mutations in this stock

Total: 56 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700017B05Rik	A	G	9: 57,166,177 (GRCm39)	W66R	probably damaging	Het
Adamts3	A	G	5: 90,009,411 (GRCm39)	V84A	possibly damaging	Het
Caap1	C	A	4: 94,389,465 (GRCm39)	E290*	probably null	Het
Cant1	G	A	11: 118,298,876 (GRCm39)	R296C	probably damaging	Het
Cdh5	A	T	8: 104,869,371 (GRCm39)	H699L	probably benign	Het
Cfdp1	T	C	8: 112,557,617 (GRCm39)	T175A	probably benign	Het
Col4a4	C	T	1: 82,471,312 (GRCm39)	G681E	unknown	Het
Comtd1	G	A	14: 21,898,861 (GRCm39)	T27I	probably benign	Het
Copg1	T	A	6: 87,869,252 (GRCm39)	V155D	probably damaging	Het
Dag1	A	G	9: 108,084,898 (GRCm39)	Y748H	possibly damaging	Het
Dmwd	T	A	7: 18,814,206 (GRCm39)	N285K	possibly damaging	Het
Dsp	A	G	13: 38,379,159 (GRCm39)	E1968G	possibly damaging	Het
Ednra	A	G	8: 78,394,004 (GRCm39)	I364T	probably damaging	Het
Elovl3	C	A	19: 46,123,120 (GRCm39)	T232K	probably damaging	Het
Ercc3	T	A	18: 32,387,296 (GRCm39)	I503N	probably damaging	Het
Ercc6	C	A	14: 32,291,580 (GRCm39)	A1008D	probably benign	Het
H2-DMb2	G	T	17: 34,367,536 (GRCm39)	V117F	probably damaging	Het
Helt	A	T	8: 46,745,470 (GRCm39)	W138R	probably damaging	Het
Itga11	A	T	9: 62,644,694 (GRCm39)	H215L	probably benign	Het
Kcnn1	T	A	8: 71,307,297 (GRCm39)	I156F	probably benign	Het
Kdm1b	A	G	13: 47,216,445 (GRCm39)	N272D	probably benign	Het
Kdm2b	T	C	5: 123,016,651 (GRCm39)	T1161A	probably damaging	Het
Lin54	A	G	5: 100,633,378 (GRCm39)	L102P	probably damaging	Het
Mlh1	A	C	9: 111,100,591 (GRCm39)	M1R	probably null	Het
Naip2	G	A	13: 100,289,068 (GRCm39)	L1165F	probably damaging	Het
Nfkbiz	A	G	16: 55,640,004 (GRCm39)	S118P	probably damaging	Het
Nkx3-2	T	A	5: 41,919,191 (GRCm39)	M266L	probably benign	Het
Npr1	T	C	3: 90,364,309 (GRCm39)	E771G	probably benign	Het
Obox8	C	T	7: 14,065,954 (GRCm39)	R188H	probably benign	Het
Odad1	T	A	7: 45,596,859 (GRCm39)	D395E	probably damaging	Het
Or5h19	A	T	16: 58,856,506 (GRCm39)	V198D	possibly damaging	Het
Or5p72	T	A	7: 108,022,609 (GRCm39)	V277E	possibly damaging	Het
Palm3	T	C	8: 84,748,159 (GRCm39)		probably null	Het
Palmd	A	T	3: 116,717,498 (GRCm39)	V333D	possibly damaging	Het
Pikfyve	A	G	1: 65,306,988 (GRCm39)	E1747G	possibly damaging	Het
Polr3a	T	C	14: 24,505,009 (GRCm39)	I1084V	possibly damaging	Het
Ranbp3l	T	A	15: 9,037,077 (GRCm39)	F127I	probably benign	Het
Rsl1d1	A	G	16: 11,019,248 (GRCm39)	F97L	possibly damaging	Het
Scaper	A	G	9: 55,771,830 (GRCm39)	V362A	probably benign	Het
Scg5	A	T	2: 113,607,210 (GRCm39)	L192*	probably null	Het
Slc34a2	T	C	5: 53,218,776 (GRCm39)	I198T	probably damaging	Het
Slc45a3	T	A	1: 131,905,932 (GRCm39)	D318E	possibly damaging	Het
Sorl1	A	G	9: 42,017,812 (GRCm39)	M105T	possibly damaging	Het
St3gal5	T	A	6: 72,126,115 (GRCm39)	I320N	probably damaging	Het
Stx17	A	G	4: 48,183,470 (GRCm39)		probably benign	Het
Syt5	C	T	7: 4,544,074 (GRCm39)		probably null	Het
Thrap3	A	G	4: 126,061,433 (GRCm39)	S774P	probably damaging	Het
Tnc	A	T	4: 63,911,443 (GRCm39)	M1376K	probably benign	Het
Tnks1bp1	T	A	2: 84,893,098 (GRCm39)	D1008E	probably benign	Het
Trav7-1	G	T	14: 52,892,761 (GRCm39)	A105S	probably damaging	Het
Vmn1r28	T	A	6: 58,242,949 (GRCm39)	V264D	probably damaging	Het
Vmn1r44	T	C	6: 89,870,821 (GRCm39)	V46A	probably benign	Het
Vmn2r10	A	G	5: 109,143,586 (GRCm39)	I788T	probably damaging	Het
Vmn2r78	T	A	7: 86,569,332 (GRCm39)	I75N	probably damaging	Het
Zfp821	T	C	8: 110,450,991 (GRCm39)	M328T	probably damaging	Het
Zfp995	G	A	17: 22,099,604 (GRCm39)	P210L	possibly damaging	Het

Other mutations in Gm10563

Allele	Source	Chr	Coord	Type	Predicted Effect
R1762:Gm10563	UTSW	4	155,720,337 (GRCm39)	intron	probably benign
R1784:Gm10563	UTSW	4	155,720,337 (GRCm39)	intron	probably benign
R1785:Gm10563	UTSW	4	155,720,337 (GRCm39)	intron	probably benign
R5263:Gm10563	UTSW	4	155,698,940 (GRCm39)	frame shift	probably null
R5291:Gm10563	UTSW	4	155,698,969 (GRCm39)	intron	probably benign
R9205:Gm10563	UTSW	4	155,720,307 (GRCm39)	small deletion	probably benign

Predicted Primers

PCR Primer
(F):5'- TACTGCCCTGTTGAGTAAGCC -3'
(R):5'- CAGATCAGATACGCCACAGTG -3'

Sequencing Primer
(F):5'- GCCCTGTTGAGTAAGCCTCTAAAAG -3'
(R):5'- GTGAAACCACACAAGTAGCCAATAGG -3'

Posted On

2016-07-06