Mutagenetix > Incidental Mutation

Incidental Mutation 'R5265:Vmn1r28'

401645

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r28

Ensembl Gene

ENSMUSG00000115705

Gene Name

vomeronasal 1 receptor 28

Synonyms

V1rc25

MMRRC Submission

042833-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.112) question?

Stock #

R5265 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

58262037-58275639 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 58265964 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Aspartic acid at position 264 (V264D)

Ref Sequence

ENSEMBL: ENSMUSP00000153785 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000176874] [ENSMUST00000226813] [ENSMUST00000227805] [ENSMUST00000228678]

AlphaFold

Q8R2C9

Predicted Effect

probably damaging
Transcript: ENSMUST00000176874
AA Change: V264D

PolyPhen 2 Score 0.984 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000135311
Gene: ENSMUSG00000094291
AA Change: V264D

Domain	Start	End	E-Value	Type
Pfam:V1R	28	293	8.4e-59	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000226813
AA Change: V264D

PolyPhen 2 Score 0.984 (Sensitivity: 0.74; Specificity: 0.96)

Predicted Effect

probably damaging
Transcript: ENSMUST00000227805
AA Change: V264D

PolyPhen 2 Score 0.984 (Sensitivity: 0.74; Specificity: 0.96)

Predicted Effect

probably damaging
Transcript: ENSMUST00000228678
AA Change: V264D

PolyPhen 2 Score 0.984 (Sensitivity: 0.74; Specificity: 0.96)

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.0%
20x: 94.7%

Validation Efficiency

100% (59/59)

Allele List at MGI

Other mutations in this stock

Total: 56 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700017B05Rik	A	G	9: 57,258,894	W66R	probably damaging	Het
Adamts3	A	G	5: 89,861,552	V84A	possibly damaging	Het
Caap1	C	A	4: 94,501,228	E290*	probably null	Het
Cant1	G	A	11: 118,408,050	R296C	probably damaging	Het
Ccdc114	T	A	7: 45,947,435	D395E	probably damaging	Het
Cdh5	A	T	8: 104,142,739	H699L	probably benign	Het
Cfdp1	T	C	8: 111,830,985	T175A	probably benign	Het
Col4a4	C	T	1: 82,493,591	G681E	unknown	Het
Comtd1	G	A	14: 21,848,793	T27I	probably benign	Het
Copg1	T	A	6: 87,892,270	V155D	probably damaging	Het
Dag1	A	G	9: 108,207,699	Y748H	possibly damaging	Het
Dmwd	T	A	7: 19,080,281	N285K	possibly damaging	Het
Dsp	A	G	13: 38,195,183	E1968G	possibly damaging	Het
Ednra	A	G	8: 77,667,375	I364T	probably damaging	Het
Elovl3	C	A	19: 46,134,681	T232K	probably damaging	Het
Ercc3	T	A	18: 32,254,243	I503N	probably damaging	Het
Ercc6	C	A	14: 32,569,623	A1008D	probably benign	Het
Gm10563	TTTC	TTTCATTC	4: 155,614,496		probably null	Het
H2-DMb2	G	T	17: 34,148,562	V117F	probably damaging	Het
Helt	A	T	8: 46,292,433	W138R	probably damaging	Het
Itga11	A	T	9: 62,737,412	H215L	probably benign	Het
Kcnn1	T	A	8: 70,854,653	I156F	probably benign	Het
Kdm1b	A	G	13: 47,062,969	N272D	probably benign	Het
Kdm2b	T	C	5: 122,878,588	T1161A	probably damaging	Het
Lin54	A	G	5: 100,485,519	L102P	probably damaging	Het
Mlh1	A	C	9: 111,271,523	M1R	probably null	Het
Naip2	G	A	13: 100,152,560	L1165F	probably damaging	Het
Nfkbiz	A	G	16: 55,819,641	S118P	probably damaging	Het
Nkx3-2	T	A	5: 41,761,848	M266L	probably benign	Het
Npr1	T	C	3: 90,457,002	E771G	probably benign	Het
Obox8	C	T	7: 14,332,029	R188H	probably benign	Het
Olfr187	A	T	16: 59,036,143	V198D	possibly damaging	Het
Olfr497	T	A	7: 108,423,402	V277E	possibly damaging	Het
Palm3	T	C	8: 84,021,530		probably null	Het
Palmd	A	T	3: 116,923,849	V333D	possibly damaging	Het
Pikfyve	A	G	1: 65,267,829	E1747G	possibly damaging	Het
Polr3a	T	C	14: 24,454,941	I1084V	possibly damaging	Het
Ranbp3l	T	A	15: 9,007,203	F127I	probably benign	Het
Rsl1d1	A	G	16: 11,201,384	F97L	possibly damaging	Het
Scaper	A	G	9: 55,864,546	V362A	probably benign	Het
Scg5	A	T	2: 113,776,865	L192*	probably null	Het
Slc34a2	T	C	5: 53,061,434	I198T	probably damaging	Het
Slc45a3	T	A	1: 131,978,194	D318E	possibly damaging	Het
Sorl1	A	G	9: 42,106,516	M105T	possibly damaging	Het
St3gal5	T	A	6: 72,149,131	I320N	probably damaging	Het
Stx17	A	G	4: 48,183,470		probably benign	Het
Syt5	C	T	7: 4,541,075		probably null	Het
Thrap3	A	G	4: 126,167,640	S774P	probably damaging	Het
Tnc	A	T	4: 63,993,206	M1376K	probably benign	Het
Tnks1bp1	T	A	2: 85,062,754	D1008E	probably benign	Het
Trav7-1	G	T	14: 52,655,304	A105S	probably damaging	Het
Vmn1r44	T	C	6: 89,893,839	V46A	probably benign	Het
Vmn2r10	A	G	5: 108,995,720	I788T	probably damaging	Het
Vmn2r78	T	A	7: 86,920,124	I75N	probably damaging	Het
Zfp821	T	C	8: 109,724,359	M328T	probably damaging	Het
Zfp995	G	A	17: 21,880,623	P210L	possibly damaging	Het

Other mutations in Vmn1r28

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01365:Vmn1r28	APN	6	58265191	missense	possibly damaging	0.76
IGL01712:Vmn1r28	APN	6	58265408	missense	probably benign	0.00
IGL01788:Vmn1r28	APN	6	58265537	missense	probably benign	0.00
IGL02629:Vmn1r28	APN	6	58265816	missense	probably benign	0.00
R0001:Vmn1r28	UTSW	6	58265717	missense	probably benign	0.00
R0009:Vmn1r28	UTSW	6	58265717	missense	probably benign	0.00
R0040:Vmn1r28	UTSW	6	58265894	missense	probably damaging	1.00
R0078:Vmn1r28	UTSW	6	58265717	missense	probably benign	0.00
R0079:Vmn1r28	UTSW	6	58265717	missense	probably benign	0.00
R0081:Vmn1r28	UTSW	6	58265717	missense	probably benign	0.00
R0164:Vmn1r28	UTSW	6	58265717	missense	probably benign	0.00
R0164:Vmn1r28	UTSW	6	58265717	missense	probably benign	0.00
R0165:Vmn1r28	UTSW	6	58265717	missense	probably benign	0.00
R0166:Vmn1r28	UTSW	6	58265717	missense	probably benign	0.00
R0167:Vmn1r28	UTSW	6	58265717	missense	probably benign	0.00
R0172:Vmn1r28	UTSW	6	58265717	missense	probably benign	0.00
R0173:Vmn1r28	UTSW	6	58265717	missense	probably benign	0.00
R0284:Vmn1r28	UTSW	6	58265717	missense	probably benign	0.00
R0288:Vmn1r28	UTSW	6	58265717	missense	probably benign	0.00
R0704:Vmn1r28	UTSW	6	58265717	missense	probably benign	0.00
R1143:Vmn1r28	UTSW	6	58265742	missense	probably benign	0.00
R1225:Vmn1r28	UTSW	6	58265966	nonsense	probably null
R1464:Vmn1r28	UTSW	6	58265232	missense	probably benign	0.05
R1464:Vmn1r28	UTSW	6	58265232	missense	probably benign	0.05
R1836:Vmn1r28	UTSW	6	58265252	missense	probably damaging	0.98
R1882:Vmn1r28	UTSW	6	58265978	missense	probably benign
R1905:Vmn1r28	UTSW	6	58265927	missense	probably benign	0.01
R4912:Vmn1r28	UTSW	6	58265540	missense	possibly damaging	0.86
R5071:Vmn1r28	UTSW	6	58265909	missense	probably benign	0.25
R5113:Vmn1r28	UTSW	6	58265858	missense	probably benign	0.12
R5261:Vmn1r28	UTSW	6	58265539	missense	probably benign	0.02
R5839:Vmn1r28	UTSW	6	58265295	missense	possibly damaging	0.94
R6180:Vmn1r28	UTSW	6	58265491	missense	probably damaging	0.99
R6643:Vmn1r28	UTSW	6	58265960	missense	probably benign	0.17
R6884:Vmn1r28	UTSW	6	58265648	missense	probably benign	0.03
R7393:Vmn1r28	UTSW	6	58265589	missense	possibly damaging	0.81
R7471:Vmn1r28	UTSW	6	58265865	nonsense	probably null
R7738:Vmn1r28	UTSW	6	58266054	missense	probably benign	0.00
R7961:Vmn1r28	UTSW	6	58265193	missense	probably benign
R8009:Vmn1r28	UTSW	6	58265193	missense	probably benign
R8156:Vmn1r28	UTSW	6	58265198	missense	probably damaging	1.00
R8167:Vmn1r28	UTSW	6	58266067	missense	noncoding transcript
R8879:Vmn1r28	UTSW	6	58265684	missense	probably benign	0.05
R9639:Vmn1r28	UTSW	6	58266011	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- TGCAGACATCAAATGCAATGC -3'
(R):5'- GCATCAAGGACACTTCTAAGAAAAG -3'

Sequencing Primer
(F):5'- TCAAATGCAATGCAAACATCTTC -3'
(R):5'- TGCTGTACAATGAACTTGAGAAG -3'

Posted On

2016-07-06