Incidental Mutation 'R5266:Rexo5'
ID401704
Institutional Source Beutler Lab
Gene Symbol Rexo5
Ensembl Gene ENSMUSG00000030924
Gene NameRNA exonuclease 5
Synonyms2610020H08Rik
MMRRC Submission 042858-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.136) question?
Stock #R5266 (G1)
Quality Score225
Status Validated
Chromosome7
Chromosomal Location119794006-119848943 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) C to A at 119844437 bp
ZygosityHeterozygous
Amino Acid Change Histidine to Glutamine at position 690 (H690Q)
Ref Sequence ENSEMBL: ENSMUSP00000102130 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000033218] [ENSMUST00000084644] [ENSMUST00000106520] [ENSMUST00000133758]
Predicted Effect probably benign
Transcript: ENSMUST00000033218
SMART Domains Protein: ENSMUSP00000033218
Gene: ENSMUSG00000030924

DomainStartEndE-ValueType
low complexity region 105 116 N/A INTRINSIC
low complexity region 123 134 N/A INTRINSIC
Pfam:RNase_T 225 330 1.4e-12 PFAM
Blast:RRM 424 463 5e-17 BLAST
Predicted Effect probably benign
Transcript: ENSMUST00000084644
SMART Domains Protein: ENSMUSP00000081694
Gene: ENSMUSG00000030924

DomainStartEndE-ValueType
EXOIII 31 189 2.72e-29 SMART
RRM 298 367 3.23e-9 SMART
Blast:RRM 393 437 2e-22 BLAST
Predicted Effect probably benign
Transcript: ENSMUST00000106520
AA Change: H690Q

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)
SMART Domains Protein: ENSMUSP00000102130
Gene: ENSMUSG00000030924
AA Change: H690Q

DomainStartEndE-ValueType
low complexity region 105 116 N/A INTRINSIC
low complexity region 123 134 N/A INTRINSIC
EXOIII 223 381 2.72e-29 SMART
RRM 491 560 3.23e-9 SMART
RRM 586 661 3.28e-2 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000133758
Predicted Effect noncoding transcript
Transcript: ENSMUST00000138689
Predicted Effect noncoding transcript
Transcript: ENSMUST00000151248
Predicted Effect probably benign
Transcript: ENSMUST00000207042
Predicted Effect probably benign
Transcript: ENSMUST00000208789
Meta Mutation Damage Score 0.0898 question?
Coding Region Coverage
  • 1x: 99.1%
  • 3x: 98.3%
  • 10x: 96.3%
  • 20x: 92.6%
Validation Efficiency 97% (56/58)
MGI Phenotype PHENOTYPE: Mice homozygous for a knock-out allele exhibit normal fertility. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 51 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4930503L19Rik T C 18: 70,458,384 N457S probably damaging Het
Asic4 G A 1: 75,450,923 G31E probably benign Het
Atp2a3 A G 11: 72,975,397 D281G probably damaging Het
Azin1 T C 15: 38,491,551 D387G probably benign Het
Bdp1 T C 13: 100,067,535 M660V probably benign Het
Catsperg2 G A 7: 29,717,066 T307M probably damaging Het
Cfap54 T G 10: 92,815,902 K3095N probably benign Het
Chl1 A G 6: 103,700,543 N706S probably damaging Het
Crym A G 7: 120,199,294 V113A probably benign Het
Cux1 A G 5: 136,312,694 S607P probably damaging Het
Cyp3a44 T C 5: 145,794,397 N198D possibly damaging Het
Dnase1l1 C T X: 74,277,038 probably null Het
Elac1 A G 18: 73,742,669 V97A probably benign Het
Erbb3 G A 10: 128,569,636 T1251M probably damaging Het
Fam198b T A 3: 79,936,603 N12K probably damaging Het
Gprc5c G T 11: 114,864,267 V257L possibly damaging Het
Hydin A G 8: 110,334,784 H316R possibly damaging Het
Ikzf3 A T 11: 98,490,580 M58K probably benign Het
Lyst T C 13: 13,660,970 Y1746H probably damaging Het
Map3k11 T A 19: 5,700,594 N613K probably benign Het
Mfng C T 15: 78,764,388 R163H probably benign Het
Mrm1 A T 11: 84,819,260 L38Q possibly damaging Het
Myo7b A G 18: 31,998,734 F470L probably damaging Het
Ndst2 G A 14: 20,724,487 R834W probably damaging Het
Olfr1173 T C 2: 88,274,221 Y276C possibly damaging Het
Olfr651 A T 7: 104,553,819 Q300L probably benign Het
Opa1 T A 16: 29,618,130 I637N probably benign Het
Padi4 A G 4: 140,746,131 V665A possibly damaging Het
Pcdh1 A G 18: 38,192,199 Y897H probably damaging Het
Pkp3 A G 7: 141,083,277 D345G probably damaging Het
Pla2g4a T C 1: 149,865,167 M366V possibly damaging Het
Pnkp C T 7: 44,862,403 S113L probably damaging Het
Pon3 T C 6: 5,240,860 D34G possibly damaging Het
Ppargc1b A T 18: 61,315,805 S133T probably damaging Het
Ppp4r3b T C 11: 29,173,309 S2P possibly damaging Het
Rbm20 A G 19: 53,813,387 T109A probably damaging Het
Scube2 C T 7: 109,809,230 G670D probably damaging Het
Sipa1l2 T C 8: 125,492,126 I157M probably damaging Het
Slbp A T 5: 33,643,866 I167N probably damaging Het
Sry C G Y: 2,662,975 Q228H unknown Het
Stk36 T C 1: 74,611,158 V283A probably benign Het
Tead1 A G 7: 112,759,466 probably benign Het
Tecpr2 G C 12: 110,915,402 W135S probably damaging Het
Tha1 A C 11: 117,869,676 S241A probably damaging Het
Ttc39a A T 4: 109,422,504 I112F probably benign Het
Vmn1r128 A T 7: 21,349,403 T11S probably benign Het
Vnn1 G A 10: 23,903,405 C404Y probably damaging Het
Wdr41 T C 13: 94,995,251 F57L probably damaging Het
Zfp975 T G 7: 42,662,230 T320P probably damaging Het
Zfp985 A T 4: 147,582,832 probably null Het
Zfpm2 T C 15: 41,099,469 S176P probably benign Het
Other mutations in Rexo5
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01308:Rexo5 APN 7 119834276 missense probably damaging 1.00
R0347:Rexo5 UTSW 7 119823896 critical splice donor site probably null
R0442:Rexo5 UTSW 7 119843285 missense probably damaging 1.00
R0589:Rexo5 UTSW 7 119845383 missense probably benign 0.00
R0980:Rexo5 UTSW 7 119823812 missense probably damaging 1.00
R1465:Rexo5 UTSW 7 119801358 critical splice donor site probably null
R1465:Rexo5 UTSW 7 119801358 critical splice donor site probably null
R1505:Rexo5 UTSW 7 119799603 nonsense probably null
R1775:Rexo5 UTSW 7 119845411 missense probably benign 0.00
R1911:Rexo5 UTSW 7 119799644 missense probably damaging 1.00
R1996:Rexo5 UTSW 7 119823857 missense probably damaging 1.00
R4168:Rexo5 UTSW 7 119827398 intron probably benign
R4169:Rexo5 UTSW 7 119827398 intron probably benign
R4402:Rexo5 UTSW 7 119834376 missense possibly damaging 0.82
R4486:Rexo5 UTSW 7 119825577 missense probably benign 0.00
R4620:Rexo5 UTSW 7 119827303 missense probably benign 0.37
R4621:Rexo5 UTSW 7 119819499 missense probably benign 0.19
R4865:Rexo5 UTSW 7 119801330 nonsense probably null
R4884:Rexo5 UTSW 7 119825551 nonsense probably null
R5171:Rexo5 UTSW 7 119823779 missense probably damaging 1.00
R5209:Rexo5 UTSW 7 119834299 nonsense probably null
R5463:Rexo5 UTSW 7 119834303 missense probably damaging 1.00
R5579:Rexo5 UTSW 7 119834403 critical splice donor site probably null
R6163:Rexo5 UTSW 7 119805247 missense probably damaging 1.00
R6305:Rexo5 UTSW 7 119828125 missense probably damaging 1.00
R7144:Rexo5 UTSW 7 119805191 missense probably damaging 1.00
R7282:Rexo5 UTSW 7 119818413 missense probably damaging 0.97
R8143:Rexo5 UTSW 7 119834261 splice site probably null
R8379:Rexo5 UTSW 7 119834285 missense probably benign 0.03
R8550:Rexo5 UTSW 7 119801345 missense probably benign 0.01
Predicted Primers PCR Primer
(F):5'- GGCCCTCGGTAACAAATGTTG -3'
(R):5'- CTAGAGTTGGCTGGAGAGTGAC -3'

Sequencing Primer
(F):5'- CTCGGTAACAAATGTTGGGATTTTTC -3'
(R):5'- TTGGCTGGAGAGTGACATGAG -3'
Posted On2016-07-06