Mutagenetix > Incidental Mutation

Incidental Mutation 'R0415:Fryl'

40222

Institutional Source

Beutler Lab

Gene Symbol

Fryl

Ensembl Gene

ENSMUSG00000070733

Gene Name

FRY like transcription coactivator

Synonyms

2510002A14Rik, 2310004H21Rik, 9030227G01Rik

MMRRC Submission

038617-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.758) question?

Stock #

R0415 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

73019987-73256619 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 73098414 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Cysteine at position 758 (Y758C)

Ref Sequence

ENSEMBL: ENSMUSP00000098687 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094700] [ENSMUST00000101127]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000094700
AA Change: Y758C

PolyPhen 2 Score 0.985 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000092289
Gene: ENSMUSG00000070733
AA Change: Y758C

Domain	Start	End	E-Value	Type
Pfam:MOR2-PAG1_N	117	649	5.7e-176	PFAM
low complexity region	873	884	N/A	INTRINSIC
Pfam:MOR2-PAG1_mid	896	1141	1.3e-5	PFAM
Pfam:MOR2-PAG1_mid	1145	1331	2e-19	PFAM
Pfam:MOR2-PAG1_mid	1351	1450	1.2e-5	PFAM
low complexity region	1476	1487	N/A	INTRINSIC
low complexity region	1530	1548	N/A	INTRINSIC
Pfam:MOR2-PAG1_mid	1590	1660	1.1e-5	PFAM
Pfam:MOR2-PAG1_mid	1725	1866	3.2e-15	PFAM
low complexity region	1973	1984	N/A	INTRINSIC
Pfam:MOR2-PAG1_C	2002	2255	9.9e-78	PFAM
low complexity region	2329	2341	N/A	INTRINSIC
low complexity region	2473	2482	N/A	INTRINSIC
low complexity region	2485	2500	N/A	INTRINSIC
low complexity region	2523	2534	N/A	INTRINSIC
coiled coil region	2625	2649	N/A	INTRINSIC
low complexity region	2827	2841	N/A	INTRINSIC
coiled coil region	2854	2893	N/A	INTRINSIC
coiled coil region	2963	2989	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000101127
AA Change: Y758C

PolyPhen 2 Score 0.985 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000098687
Gene: ENSMUSG00000070733
AA Change: Y758C

Domain	Start	End	E-Value	Type
Pfam:MOR2-PAG1_N	116	649	3e-172	PFAM
low complexity region	873	884	N/A	INTRINSIC
Pfam:MOR2-PAG1_mid	898	1115	7.8e-6	PFAM
Pfam:MOR2-PAG1_mid	1147	1331	9.5e-19	PFAM
Pfam:MOR2-PAG1_mid	1351	1503	1.1e-5	PFAM
low complexity region	1530	1548	N/A	INTRINSIC
Pfam:MOR2-PAG1_mid	1589	1664	6e-6	PFAM
Pfam:MOR2-PAG1_mid	1725	1866	1.6e-14	PFAM
low complexity region	1973	1984	N/A	INTRINSIC
Pfam:MOR2-PAG1_C	1998	2260	4.4e-76	PFAM
low complexity region	2329	2341	N/A	INTRINSIC
low complexity region	2473	2482	N/A	INTRINSIC
low complexity region	2485	2500	N/A	INTRINSIC
low complexity region	2523	2534	N/A	INTRINSIC
coiled coil region	2625	2649	N/A	INTRINSIC
low complexity region	2827	2841	N/A	INTRINSIC
coiled coil region	2854	2893	N/A	INTRINSIC
coiled coil region	2963	2989	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000153923

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000201405

SMART Domains

Protein: ENSMUSP00000144346
Gene: ENSMUSG00000070733

Domain	Start	End	E-Value	Type
low complexity region	103	114	N/A	INTRINSIC

Meta Mutation Damage Score

0.5998

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.7%
20x: 93.6%

Validation Efficiency

97% (99/102)

MGI Phenotype

PHENOTYPE: Most mice homozygous for a knock-out allele exhibit postnatal lethality and defects in kidney development; rare survivors display growth retardation, decreased body weight, and premature death associated with chronic hydronephrosis. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 98 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933427G23Rik	A	T	5: 23,831,050 (GRCm38)		noncoding transcript	Het
Acox2	A	G	14: 8,243,835 (GRCm38)		probably benign	Het
Adgb	T	C	10: 10,431,067 (GRCm38)		probably null	Het
Adgra3	C	A	5: 49,961,757 (GRCm38)		probably benign	Het
Adgre4	G	A	17: 55,852,288 (GRCm38)	V658I	probably benign	Het
Ahnak	A	G	19: 9,012,871 (GRCm38)		probably benign	Het
Anapc2	A	G	2: 25,278,325 (GRCm38)	T159A	probably damaging	Het
Arfgef3	A	G	10: 18,613,127 (GRCm38)		probably benign	Het
Atf7ip	C	T	6: 136,560,012 (GRCm38)	S81L	possibly damaging	Het
Cacna1i	A	G	15: 80,368,830 (GRCm38)		probably benign	Het
Camk1	A	T	6: 113,341,891 (GRCm38)	Y20*	probably null	Het
Ccdc40	T	C	11: 119,232,118 (GRCm38)	Y249H	possibly damaging	Het
Cd109	T	A	9: 78,712,615 (GRCm38)	S1380T	probably benign	Het
Cfap57	A	T	4: 118,569,431 (GRCm38)	L1107Q	possibly damaging	Het
Col6a4	C	T	9: 106,075,080 (GRCm38)	V540I	probably damaging	Het
Cst9	T	A	2: 148,838,442 (GRCm38)		probably benign	Het
Cul5	C	T	9: 53,667,070 (GRCm38)	V73I	probably benign	Het
Cxcl16	T	A	11: 70,458,748 (GRCm38)	K84*	probably null	Het
Cyp2c29	T	C	19: 39,329,095 (GRCm38)		probably benign	Het
D6Ertd527e	C	G	6: 87,111,524 (GRCm38)	T223S	unknown	Het
Dip2c	C	T	13: 9,568,289 (GRCm38)		probably benign	Het
Dis3	A	T	14: 99,087,456 (GRCm38)	I513N	probably damaging	Het
Dnajc16	A	T	4: 141,789,048 (GRCm38)	L3*	probably null	Het
Dopey1	T	A	9: 86,506,502 (GRCm38)	L480M	probably damaging	Het
Eml6	A	G	11: 29,749,392 (GRCm38)	V1787A	possibly damaging	Het
Etnk1	A	G	6: 143,180,774 (GRCm38)	N115S	probably damaging	Het
Gbp4	G	A	5: 105,121,106 (GRCm38)	R394C	possibly damaging	Het
Ggnbp2	A	C	11: 84,833,225 (GRCm38)		probably benign	Het
Gm7137	A	G	10: 77,788,173 (GRCm38)		probably benign	Het
Gstm2	T	A	3: 107,984,006 (GRCm38)	Q132L	probably benign	Het
Habp2	T	C	19: 56,317,717 (GRCm38)		probably benign	Het
Hectd2	T	C	19: 36,584,884 (GRCm38)		probably benign	Het
Htr6	A	G	4: 139,062,081 (GRCm38)	I291T	possibly damaging	Het
Ighg2c	T	C	12: 113,287,910 (GRCm38)	D199G	unknown	Het
Itih2	A	G	2: 10,105,615 (GRCm38)		probably benign	Het
Kcnab2	A	G	4: 152,395,136 (GRCm38)	F248S	probably benign	Het
Kcnc4	T	C	3: 107,445,433 (GRCm38)	K610E	probably damaging	Het
Kcnk16	T	A	14: 20,262,975 (GRCm38)		probably null	Het
Kndc1	C	T	7: 139,930,124 (GRCm38)	T1293I	probably damaging	Het
Lcp1	A	T	14: 75,227,006 (GRCm38)	I556F	possibly damaging	Het
Lrrc8d	T	C	5: 105,811,865 (GRCm38)	L47P	probably damaging	Het
Lyst	T	C	13: 13,711,610 (GRCm38)		probably benign	Het
Macrod2	G	A	2: 142,210,145 (GRCm38)		probably null	Het
Micalcl	C	T	7: 112,381,028 (GRCm38)	R70C	probably damaging	Het
Mllt3	ACTGCTGCTGCTGCTGCTGCT	ACTGCTGCTGCTGCTGCT	4: 87,841,339 (GRCm38)		probably benign	Het
Msh3	A	G	13: 92,346,786 (GRCm38)	V283A	possibly damaging	Het
Nup205	T	C	6: 35,214,634 (GRCm38)		probably benign	Het
Nxpe2	T	C	9: 48,326,614 (GRCm38)	T114A	probably damaging	Het
Olfr1023	A	T	2: 85,887,438 (GRCm38)	I213F	possibly damaging	Het
Olfr1034	A	T	2: 86,047,055 (GRCm38)	H191L	probably benign	Het
Olfr229	A	G	9: 39,909,983 (GRCm38)	Y60C	probably damaging	Het
Olfr78	C	T	7: 102,742,087 (GRCm38)	M305I	probably benign	Het
Olfr893	A	T	9: 38,209,973 (GRCm38)	M305L	probably benign	Het
Pard3	G	A	8: 127,610,566 (GRCm38)	G1221D	probably damaging	Het
Pax5	G	A	4: 44,691,886 (GRCm38)	A120V	probably damaging	Het
Pcsk6	C	T	7: 66,033,874 (GRCm38)	R746C	probably damaging	Het
Pif1	G	A	9: 65,588,051 (GRCm38)	C81Y	probably benign	Het
Plcb1	A	G	2: 135,337,499 (GRCm38)	Y609C	probably damaging	Het
Plcd4	C	A	1: 74,552,097 (GRCm38)	S217Y	probably damaging	Het
Plxna1	G	A	6: 89,357,336 (GRCm38)	H104Y	probably benign	Het
Polr2k	A	G	15: 36,175,456 (GRCm38)	Y45C	probably damaging	Het
Pqlc3	C	A	12: 16,997,710 (GRCm38)		probably benign	Het
Prex1	A	G	2: 166,586,699 (GRCm38)		probably benign	Het
Pth2r	A	G	1: 65,388,439 (GRCm38)	M424V	probably benign	Het
Pygm	A	G	19: 6,391,366 (GRCm38)	R464G	probably benign	Het
Rad51c	A	G	11: 87,397,655 (GRCm38)	L234P	probably damaging	Het
Rnf145	A	G	11: 44,525,138 (GRCm38)	Y60C	probably damaging	Het
Rnf167	T	C	11: 70,649,699 (GRCm38)	I135T	probably damaging	Het
Rnf213	A	T	11: 119,414,469 (GRCm38)	I509F	probably damaging	Het
Ryr2	T	C	13: 11,869,156 (GRCm38)	S213G	probably damaging	Het
Selenbp1	T	C	3: 94,936,913 (GRCm38)	V27A	possibly damaging	Het
Selenof	T	G	3: 144,577,692 (GRCm38)	L14R	probably damaging	Het
Sfswap	A	T	5: 129,504,126 (GRCm38)	D121V	probably damaging	Het
Slc25a34	C	A	4: 141,620,469 (GRCm38)	M300I	possibly damaging	Het
Slc34a3	T	G	2: 25,229,110 (GRCm38)	T583P	probably benign	Het
Smg1	C	A	7: 118,182,468 (GRCm38)	A1199S	probably benign	Het
Spint1	A	G	2: 119,245,615 (GRCm38)	T231A	probably damaging	Het
Sptbn1	A	C	11: 30,149,576 (GRCm38)	N229K	probably damaging	Het
Sult2b1	A	G	7: 45,730,092 (GRCm38)		probably benign	Het
Tas2r123	A	T	6: 132,847,838 (GRCm38)	M233L	probably damaging	Het
Tbcel	C	A	9: 42,444,500 (GRCm38)	C139F	probably benign	Het
Thbs2	A	C	17: 14,679,973 (GRCm38)	S573A	probably benign	Het
Tmem132c	A	G	5: 127,563,705 (GRCm38)	E980G	probably damaging	Het
Tmem247	G	T	17: 86,922,322 (GRCm38)	C197F	probably damaging	Het
Tmem251	T	A	12: 102,744,876 (GRCm38)	Y119*	probably null	Het
Tmem43	C	A	6: 91,482,318 (GRCm38)	P257Q	probably benign	Het
Tmprss13	A	G	9: 45,337,132 (GRCm38)		probably null	Het
Trove2	G	T	1: 143,760,075 (GRCm38)	N444K	probably benign	Het
Ubr5	T	C	15: 37,972,980 (GRCm38)	T2626A	probably damaging	Het
Vmn1r196	T	A	13: 22,293,836 (GRCm38)	V215D	probably damaging	Het
Vmn1r22	G	T	6: 57,900,332 (GRCm38)	T220K	probably benign	Het
Vmn2r116	G	A	17: 23,387,279 (GRCm38)	M388I	possibly damaging	Het
Vmn2r74	A	C	7: 85,961,410 (GRCm38)	C25G	probably damaging	Het
Xndc1	T	C	7: 102,080,616 (GRCm38)		probably benign	Het
Zfp282	T	A	6: 47,905,053 (GRCm38)	I558N	possibly damaging	Het
Zfp282	A	G	6: 47,897,881 (GRCm38)	D340G	probably damaging	Het
Zfp316	T	A	5: 143,264,491 (GRCm38)	T56S	unknown	Het
Zfp345	A	G	2: 150,474,559 (GRCm38)		probably benign	Het

Other mutations in Fryl

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00819:Fryl	APN	5	73,148,108 (GRCm38)	missense	possibly damaging	0.92
IGL01518:Fryl	APN	5	73,086,962 (GRCm38)	missense	possibly damaging	0.76
IGL01545:Fryl	APN	5	73,054,597 (GRCm38)	missense	probably damaging	1.00
IGL01646:Fryl	APN	5	73,022,501 (GRCm38)	critical splice donor site	probably null
IGL01938:Fryl	APN	5	73,122,364 (GRCm38)	missense	probably damaging	0.98
IGL01962:Fryl	APN	5	73,032,791 (GRCm38)	missense	possibly damaging	0.62
IGL02064:Fryl	APN	5	73,124,769 (GRCm38)	unclassified	probably benign
IGL02148:Fryl	APN	5	73,075,959 (GRCm38)	missense	probably benign	0.35
IGL02418:Fryl	APN	5	73,110,176 (GRCm38)	splice site	probably benign
IGL02431:Fryl	APN	5	73,098,308 (GRCm38)	missense	probably benign	0.02
IGL02513:Fryl	APN	5	73,065,293 (GRCm38)	missense	probably damaging	1.00
IGL02557:Fryl	APN	5	73,098,393 (GRCm38)	missense	probably damaging	1.00
IGL02625:Fryl	APN	5	73,069,877 (GRCm38)	intron	probably benign
IGL02642:Fryl	APN	5	73,095,466 (GRCm38)	missense	probably benign
IGL02657:Fryl	APN	5	73,054,860 (GRCm38)	missense	probably benign	0.01
IGL02706:Fryl	APN	5	73,093,163 (GRCm38)	missense	probably benign	0.45
IGL03022:Fryl	APN	5	73,059,383 (GRCm38)	missense	possibly damaging	0.82
IGL03144:Fryl	APN	5	73,101,455 (GRCm38)	missense	probably null	0.22
IGL03155:Fryl	APN	5	73,076,695 (GRCm38)	missense	probably benign
IGL03183:Fryl	APN	5	73,076,695 (GRCm38)	missense	probably benign
IGL03275:Fryl	APN	5	73,148,033 (GRCm38)	missense	possibly damaging	0.47
IGL03310:Fryl	APN	5	73,136,316 (GRCm38)	splice site	probably benign
IGL03341:Fryl	APN	5	73,076,695 (GRCm38)	missense	probably benign
IGL03343:Fryl	APN	5	73,076,695 (GRCm38)	missense	probably benign
IGL03350:Fryl	APN	5	73,133,306 (GRCm38)	missense	probably damaging	0.99
IGL03357:Fryl	APN	5	73,054,059 (GRCm38)	missense	probably damaging	1.00
IGL03374:Fryl	APN	5	73,110,281 (GRCm38)	splice site	probably benign
IGL03375:Fryl	APN	5	73,088,449 (GRCm38)	missense	possibly damaging	0.91
bedeviled	UTSW	5	73,059,500 (GRCm38)	missense	probably damaging	1.00
Besotted	UTSW	5	73,072,912 (GRCm38)	missense	probably damaging	1.00
R0062:Fryl	UTSW	5	73,022,278 (GRCm38)	missense	probably benign	0.02
R0062:Fryl	UTSW	5	73,022,278 (GRCm38)	missense	probably benign	0.02
R0308:Fryl	UTSW	5	73,041,604 (GRCm38)	splice site	probably benign
R0312:Fryl	UTSW	5	73,072,888 (GRCm38)	missense	probably damaging	1.00
R0440:Fryl	UTSW	5	73,086,972 (GRCm38)	missense	possibly damaging	0.91
R0446:Fryl	UTSW	5	73,097,417 (GRCm38)	missense	possibly damaging	0.91
R0566:Fryl	UTSW	5	73,064,497 (GRCm38)	splice site	probably benign
R0567:Fryl	UTSW	5	73,065,391 (GRCm38)	missense	possibly damaging	0.50
R0606:Fryl	UTSW	5	73,124,734 (GRCm38)	missense	probably benign	0.15
R0619:Fryl	UTSW	5	73,068,731 (GRCm38)	missense	probably benign	0.22
R0654:Fryl	UTSW	5	73,083,372 (GRCm38)	missense	probably benign	0.17
R0658:Fryl	UTSW	5	73,065,359 (GRCm38)	missense	probably damaging	1.00
R0707:Fryl	UTSW	5	73,083,372 (GRCm38)	missense	probably benign	0.17
R0744:Fryl	UTSW	5	73,089,081 (GRCm38)	unclassified	probably benign
R0745:Fryl	UTSW	5	73,071,126 (GRCm38)	missense	probably damaging	0.96
R0833:Fryl	UTSW	5	73,089,081 (GRCm38)	unclassified	probably benign
R0885:Fryl	UTSW	5	73,089,196 (GRCm38)	missense	probably damaging	0.97
R0894:Fryl	UTSW	5	73,041,332 (GRCm38)	splice site	probably benign
R1076:Fryl	UTSW	5	73,124,673 (GRCm38)	unclassified	probably benign
R1241:Fryl	UTSW	5	73,110,271 (GRCm38)	missense	probably damaging	1.00
R1241:Fryl	UTSW	5	73,064,925 (GRCm38)	splice site	probably benign
R1394:Fryl	UTSW	5	73,072,912 (GRCm38)	missense	probably damaging	1.00
R1395:Fryl	UTSW	5	73,072,912 (GRCm38)	missense	probably damaging	1.00
R1608:Fryl	UTSW	5	73,074,751 (GRCm38)	nonsense	probably null
R1664:Fryl	UTSW	5	73,059,435 (GRCm38)	missense	probably damaging	1.00
R1745:Fryl	UTSW	5	73,032,861 (GRCm38)	splice site	probably benign
R1937:Fryl	UTSW	5	73,133,367 (GRCm38)	missense	probably damaging	1.00
R1969:Fryl	UTSW	5	73,098,266 (GRCm38)	missense	probably benign	0.18
R1993:Fryl	UTSW	5	73,108,493 (GRCm38)	missense	probably damaging	1.00
R1994:Fryl	UTSW	5	73,108,493 (GRCm38)	missense	probably damaging	1.00
R2029:Fryl	UTSW	5	73,022,122 (GRCm38)	nonsense	probably null
R2036:Fryl	UTSW	5	73,107,962 (GRCm38)	critical splice donor site	probably null
R2036:Fryl	UTSW	5	73,022,544 (GRCm38)	missense	probably benign
R2088:Fryl	UTSW	5	73,065,461 (GRCm38)	missense	probably benign	0.02
R2105:Fryl	UTSW	5	73,122,299 (GRCm38)	missense	probably benign
R2106:Fryl	UTSW	5	73,098,331 (GRCm38)	missense	probably damaging	1.00
R2186:Fryl	UTSW	5	73,064,975 (GRCm38)	missense	probably damaging	1.00
R2239:Fryl	UTSW	5	73,108,547 (GRCm38)	missense	probably damaging	0.99
R2256:Fryl	UTSW	5	73,072,844 (GRCm38)	missense	possibly damaging	0.47
R2257:Fryl	UTSW	5	73,072,844 (GRCm38)	missense	possibly damaging	0.47
R2280:Fryl	UTSW	5	73,041,364 (GRCm38)	missense	possibly damaging	0.47
R2281:Fryl	UTSW	5	73,041,364 (GRCm38)	missense	possibly damaging	0.47
R2911:Fryl	UTSW	5	73,050,456 (GRCm38)	missense	probably damaging	0.99
R3019:Fryl	UTSW	5	73,082,850 (GRCm38)	missense	probably benign	0.01
R3416:Fryl	UTSW	5	73,108,074 (GRCm38)	missense	possibly damaging	0.84
R3783:Fryl	UTSW	5	73,101,476 (GRCm38)	missense	probably benign
R3787:Fryl	UTSW	5	73,101,476 (GRCm38)	missense	probably benign
R3837:Fryl	UTSW	5	73,071,265 (GRCm38)	missense	probably benign	0.03
R3969:Fryl	UTSW	5	73,112,423 (GRCm38)	missense	probably damaging	0.97
R4387:Fryl	UTSW	5	73,086,560 (GRCm38)	missense	possibly damaging	0.91
R4502:Fryl	UTSW	5	73,088,397 (GRCm38)	missense	probably damaging	1.00
R4658:Fryl	UTSW	5	73,081,053 (GRCm38)	missense	probably damaging	1.00
R4664:Fryl	UTSW	5	73,090,679 (GRCm38)	missense	possibly damaging	0.80
R4690:Fryl	UTSW	5	73,100,293 (GRCm38)	missense	probably benign
R4700:Fryl	UTSW	5	73,065,538 (GRCm38)	missense	possibly damaging	0.88
R4709:Fryl	UTSW	5	73,080,972 (GRCm38)	missense	probably benign	0.03
R4807:Fryl	UTSW	5	73,041,362 (GRCm38)	missense	probably benign	0.00
R4912:Fryl	UTSW	5	73,068,782 (GRCm38)	frame shift	probably null
R4948:Fryl	UTSW	5	73,089,130 (GRCm38)	missense	probably benign	0.08
R4959:Fryl	UTSW	5	73,035,058 (GRCm38)	missense	probably benign	0.00
R5062:Fryl	UTSW	5	73,075,893 (GRCm38)	missense	possibly damaging	0.89
R5067:Fryl	UTSW	5	73,057,755 (GRCm38)	missense	probably benign	0.13
R5071:Fryl	UTSW	5	73,074,767 (GRCm38)	missense	probably damaging	0.99
R5072:Fryl	UTSW	5	73,074,767 (GRCm38)	missense	probably damaging	0.99
R5073:Fryl	UTSW	5	73,074,767 (GRCm38)	missense	probably damaging	0.99
R5074:Fryl	UTSW	5	73,074,767 (GRCm38)	missense	probably damaging	0.99
R5139:Fryl	UTSW	5	73,090,718 (GRCm38)	missense	probably damaging	1.00
R5172:Fryl	UTSW	5	73,101,673 (GRCm38)	missense	possibly damaging	0.95
R5187:Fryl	UTSW	5	73,086,600 (GRCm38)	missense	possibly damaging	0.95
R5272:Fryl	UTSW	5	73,065,136 (GRCm38)	nonsense	probably null
R5275:Fryl	UTSW	5	73,112,791 (GRCm38)	missense	probably damaging	1.00
R5295:Fryl	UTSW	5	73,112,791 (GRCm38)	missense	probably damaging	1.00
R5344:Fryl	UTSW	5	73,104,774 (GRCm38)	missense	probably damaging	1.00
R5355:Fryl	UTSW	5	73,073,904 (GRCm38)	missense	probably damaging	1.00
R5716:Fryl	UTSW	5	73,100,465 (GRCm38)	missense	probably benign
R5778:Fryl	UTSW	5	73,072,778 (GRCm38)	missense	probably damaging	1.00
R5810:Fryl	UTSW	5	73,090,755 (GRCm38)	missense	probably benign	0.06
R5934:Fryl	UTSW	5	73,090,717 (GRCm38)	missense	probably damaging	1.00
R5948:Fryl	UTSW	5	73,097,372 (GRCm38)	critical splice donor site	probably null
R6005:Fryl	UTSW	5	73,083,295 (GRCm38)	missense	probably damaging	1.00
R6026:Fryl	UTSW	5	73,099,997 (GRCm38)	missense	probably benign	0.04
R6045:Fryl	UTSW	5	73,118,551 (GRCm38)	missense	probably damaging	0.99
R6185:Fryl	UTSW	5	73,112,788 (GRCm38)	missense	probably benign	0.43
R6247:Fryl	UTSW	5	73,065,481 (GRCm38)	missense	probably damaging	0.98
R6294:Fryl	UTSW	5	73,191,759 (GRCm38)	intron	probably benign
R6310:Fryl	UTSW	5	73,191,761 (GRCm38)	intron	probably benign
R6429:Fryl	UTSW	5	73,090,751 (GRCm38)	missense	possibly damaging	0.84
R6568:Fryl	UTSW	5	73,059,516 (GRCm38)	missense	probably damaging	1.00
R6636:Fryl	UTSW	5	73,133,312 (GRCm38)	missense	probably benign	0.01
R6664:Fryl	UTSW	5	73,132,481 (GRCm38)	missense	probably damaging	1.00
R6732:Fryl	UTSW	5	73,054,781 (GRCm38)	missense	probably damaging	1.00
R6750:Fryl	UTSW	5	73,022,232 (GRCm38)	missense	probably damaging	1.00
R6805:Fryl	UTSW	5	73,065,094 (GRCm38)	missense	probably benign	0.03
R6823:Fryl	UTSW	5	73,065,217 (GRCm38)	missense	probably damaging	0.99
R6855:Fryl	UTSW	5	73,059,500 (GRCm38)	missense	probably damaging	1.00
R6858:Fryl	UTSW	5	73,065,032 (GRCm38)	missense	probably damaging	1.00
R6868:Fryl	UTSW	5	73,068,803 (GRCm38)	missense	probably damaging	1.00
R6898:Fryl	UTSW	5	73,022,142 (GRCm38)	missense	probably damaging	0.96
R6908:Fryl	UTSW	5	73,022,211 (GRCm38)	missense	probably damaging	1.00
R6958:Fryl	UTSW	5	73,073,929 (GRCm38)	missense	possibly damaging	0.89
R6980:Fryl	UTSW	5	73,050,430 (GRCm38)	missense	probably benign	0.06
R7036:Fryl	UTSW	5	73,055,608 (GRCm38)	missense	probably benign	0.03
R7065:Fryl	UTSW	5	73,090,756 (GRCm38)	missense	probably damaging	0.96
R7097:Fryl	UTSW	5	73,073,908 (GRCm38)	missense	probably benign	0.31
R7171:Fryl	UTSW	5	73,122,310 (GRCm38)	missense	probably damaging	0.97
R7191:Fryl	UTSW	5	73,072,912 (GRCm38)	missense	probably damaging	1.00
R7207:Fryl	UTSW	5	73,065,095 (GRCm38)	missense	probably benign
R7236:Fryl	UTSW	5	73,108,478 (GRCm38)	missense	possibly damaging	0.66
R7334:Fryl	UTSW	5	73,047,496 (GRCm38)	splice site	probably null
R7425:Fryl	UTSW	5	73,104,748 (GRCm38)	missense	probably damaging	1.00
R7452:Fryl	UTSW	5	73,023,988 (GRCm38)	missense	probably damaging	1.00
R7479:Fryl	UTSW	5	73,097,561 (GRCm38)	missense	possibly damaging	0.71
R7535:Fryl	UTSW	5	73,098,196 (GRCm38)	missense	probably benign	0.15
R7538:Fryl	UTSW	5	73,022,676 (GRCm38)	missense	probably benign	0.09
R7544:Fryl	UTSW	5	73,081,039 (GRCm38)	missense	probably benign
R7548:Fryl	UTSW	5	73,191,762 (GRCm38)	missense	unknown
R7565:Fryl	UTSW	5	73,033,720 (GRCm38)	missense	probably benign	0.18
R7572:Fryl	UTSW	5	73,088,396 (GRCm38)	missense	possibly damaging	0.91
R7582:Fryl	UTSW	5	73,022,500 (GRCm38)	critical splice donor site	probably null
R7630:Fryl	UTSW	5	73,110,245 (GRCm38)	missense	possibly damaging	0.62
R7774:Fryl	UTSW	5	73,083,384 (GRCm38)	missense	probably benign	0.12
R7777:Fryl	UTSW	5	73,071,298 (GRCm38)	missense	probably damaging	0.98
R7917:Fryl	UTSW	5	73,054,532 (GRCm38)	missense	probably damaging	1.00
R7920:Fryl	UTSW	5	73,101,807 (GRCm38)	splice site	probably null
R8110:Fryl	UTSW	5	73,133,277 (GRCm38)	missense	probably benign	0.10
R8120:Fryl	UTSW	5	73,071,184 (GRCm38)	missense	probably benign	0.01
R8143:Fryl	UTSW	5	73,050,339 (GRCm38)	missense	probably benign	0.00
R8207:Fryl	UTSW	5	73,100,500 (GRCm38)	splice site	probably null
R8263:Fryl	UTSW	5	73,081,005 (GRCm38)	missense	probably damaging	1.00
R8350:Fryl	UTSW	5	73,068,730 (GRCm38)	missense	probably benign
R8359:Fryl	UTSW	5	73,075,933 (GRCm38)	missense	probably benign	0.39
R8387:Fryl	UTSW	5	73,136,320 (GRCm38)	critical splice donor site	probably null
R8403:Fryl	UTSW	5	73,118,447 (GRCm38)	makesense	probably null
R8450:Fryl	UTSW	5	73,068,730 (GRCm38)	missense	probably benign
R8514:Fryl	UTSW	5	73,085,356 (GRCm38)	missense	probably benign
R8536:Fryl	UTSW	5	73,100,353 (GRCm38)	missense	probably damaging	0.99
R8703:Fryl	UTSW	5	73,090,654 (GRCm38)	missense	probably damaging	0.99
R8708:Fryl	UTSW	5	73,132,562 (GRCm38)	missense	probably benign	0.01
R8783:Fryl	UTSW	5	73,068,842 (GRCm38)	missense	probably benign	0.45
R9028:Fryl	UTSW	5	73,098,266 (GRCm38)	missense	probably benign	0.18
R9045:Fryl	UTSW	5	73,024,775 (GRCm38)	missense
R9063:Fryl	UTSW	5	73,081,003 (GRCm38)	missense	possibly damaging	0.70
R9096:Fryl	UTSW	5	73,108,577 (GRCm38)	missense	probably benign	0.01
R9244:Fryl	UTSW	5	73,191,519 (GRCm38)	intron	probably benign
R9345:Fryl	UTSW	5	73,050,411 (GRCm38)	missense	probably benign
R9381:Fryl	UTSW	5	73,083,294 (GRCm38)	missense	probably benign	0.24
R9386:Fryl	UTSW	5	73,191,809 (GRCm38)	missense	unknown
R9401:Fryl	UTSW	5	73,065,220 (GRCm38)	nonsense	probably null
R9497:Fryl	UTSW	5	73,057,791 (GRCm38)	missense
R9514:Fryl	UTSW	5	73,104,772 (GRCm38)	missense	probably damaging	1.00
R9570:Fryl	UTSW	5	73,022,155 (GRCm38)	missense	probably benign	0.02
R9654:Fryl	UTSW	5	73,118,458 (GRCm38)	missense	probably benign
R9665:Fryl	UTSW	5	73,064,956 (GRCm38)	missense	probably damaging	1.00
R9685:Fryl	UTSW	5	73,059,536 (GRCm38)	missense	probably damaging	0.99
R9798:Fryl	UTSW	5	73,035,059 (GRCm38)	missense	probably benign
Z1088:Fryl	UTSW	5	73,090,738 (GRCm38)	missense	probably damaging	1.00
Z1088:Fryl	UTSW	5	73,090,709 (GRCm38)	missense	probably damaging	0.99
Z1176:Fryl	UTSW	5	73,072,837 (GRCm38)	missense	probably benign
Z1177:Fryl	UTSW	5	73,041,595 (GRCm38)	critical splice acceptor site	probably null

Predicted Primers

PCR Primer
(F):5'- TGCGAACATCCACGCATAGCTC -3'
(R):5'- CGGTGGAAATGTCAGAGACGCATTC -3'

Sequencing Primer
(F):5'- CACGCATAGCTCACGGC -3'
(R):5'- GAGACGCATTCCTTCCTTAAAC -3'

Posted On

2013-05-23