Mutagenetix > Incidental Mutation

Incidental Mutation 'R5221:Or2d3c'

402321

Institutional Source

Beutler Lab

Gene Symbol

Or2d3c

Ensembl Gene

ENSMUSG00000062553

Gene Name

olfactory receptor family 2 subfamily D member 3C

Synonyms

MOR260-7, GA_x6K02T2PBJ9-9307730-9306814, Olfr709

MMRRC Submission

042794-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.369) question?

Stock #

R5221 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

106525744-106526664 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 106526268 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Threonine at position 133 (S133T)

Ref Sequence

ENSEMBL: ENSMUSP00000150392 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000072368] [ENSMUST00000215468]

AlphaFold

A0A140T8J9

Predicted Effect

probably benign
Transcript: ENSMUST00000072368
AA Change: S133T

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000072205
Gene: ENSMUSG00000062553
AA Change: S133T

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	307	7.8e-58	PFAM
Pfam:7TM_GPCR_Srsx	35	282	8.4e-9	PFAM
Pfam:7tm_1	41	290	5.9e-27	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000215468
AA Change: S133T

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

Meta Mutation Damage Score

0.0945

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.5%
20x: 95.9%

Validation Efficiency

98% (53/54)

Allele List at MGI

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adrm1b	T	C	3: 92,335,815 (GRCm39)	I296V	probably benign	Het
Antkmt	T	C	17: 26,010,613 (GRCm39)	N67S	possibly damaging	Het
Arhgap26	T	A	18: 39,243,525 (GRCm39)	Y134*	probably null	Het
Asap2	A	G	12: 21,263,191 (GRCm39)	I269V	probably benign	Het
Bean1	A	T	8: 104,941,784 (GRCm39)	D34V	probably damaging	Het
C8a	C	T	4: 104,703,122 (GRCm39)	V312M	probably damaging	Het
Ccser1	T	C	6: 61,289,075 (GRCm39)	S413P	probably damaging	Het
Col4a2	A	G	8: 11,498,225 (GRCm39)	N1678S	probably benign	Het
Coq2	G	A	5: 100,805,698 (GRCm39)	H313Y	possibly damaging	Het
Dsg1a	T	A	18: 20,457,071 (GRCm39)	M148K	possibly damaging	Het
Farp2	T	A	1: 93,504,140 (GRCm39)	C306S	probably damaging	Het
Frem2	A	G	3: 53,493,032 (GRCm39)	V1828A	probably damaging	Het
Gm6457	T	C	18: 14,703,174 (GRCm39)		noncoding transcript	Het
Gmip	T	G	8: 70,266,785 (GRCm39)	V300G	probably damaging	Het
Gstp3	A	G	19: 4,107,607 (GRCm39)	S186P	probably damaging	Het
Gvin-ps3	G	C	7: 105,683,181 (GRCm39)		noncoding transcript	Het
Kl	G	T	5: 150,912,616 (GRCm39)	L788F	probably damaging	Het
Lin9	T	A	1: 180,496,763 (GRCm39)	L351I	probably benign	Het
Lrp1b	A	C	2: 41,002,994 (GRCm39)	S1932A	possibly damaging	Het
Lrrc36	A	G	8: 106,170,488 (GRCm39)	T234A	probably damaging	Het
Mafa	T	A	15: 75,618,891 (GRCm39)	Q294L	possibly damaging	Het
Mesp2	C	T	7: 79,461,467 (GRCm39)	T264I	possibly damaging	Het
Mindy4	A	G	6: 55,201,092 (GRCm39)	Y259C	probably benign	Het
Or1e16	AGCGGTCGTAGGC	AGC	11: 73,286,480 (GRCm39)		probably null	Het
Or5as1	T	C	2: 86,980,825 (GRCm39)	Y60C	probably damaging	Het
Or5m13b	A	G	2: 85,754,493 (GRCm39)	N294D	probably damaging	Het
Or8b48	A	T	9: 38,493,148 (GRCm39)	T192S	probably damaging	Het
Pcm1	T	C	8: 41,741,193 (GRCm39)		probably null	Het
Plxna1	T	C	6: 89,297,998 (GRCm39)	D1760G	probably damaging	Het
Polq	T	A	16: 36,862,540 (GRCm39)	D345E	probably damaging	Het
Ppp1r14a	T	A	7: 28,988,926 (GRCm39)	I56N	probably damaging	Het
Ppp1r1a	G	A	15: 103,441,477 (GRCm39)	P79S	probably damaging	Het
Ptprf	C	T	4: 118,082,305 (GRCm39)	R1010H	probably benign	Het
Rai1	A	G	11: 60,081,423 (GRCm39)	E1829G	probably damaging	Het
Rmdn3	A	G	2: 118,986,935 (GRCm39)	Y31H	probably damaging	Het
Rrh	T	C	3: 129,609,280 (GRCm39)	S69G	probably damaging	Het
Skint6	A	T	4: 112,752,121 (GRCm39)		probably null	Het
Slc22a27	C	G	19: 7,843,303 (GRCm39)	A359P	probably damaging	Het
Sltm	T	G	9: 70,486,685 (GRCm39)	L450R	probably damaging	Het
Sufu	T	C	19: 46,439,404 (GRCm39)		probably null	Het
Tmem68	T	C	4: 3,560,561 (GRCm39)	T208A	possibly damaging	Het
Tnc	T	A	4: 63,911,534 (GRCm39)	R1346*	probably null	Het
Tpbg	T	C	9: 85,726,478 (GRCm39)	L149P	probably damaging	Het
Trappc14	C	T	5: 138,260,502 (GRCm39)	S308N	probably benign	Het
Ubtf	G	A	11: 102,198,816 (GRCm39)	Q585*	probably null	Het
Vmn2r68	A	T	7: 84,871,085 (GRCm39)	C733S	probably damaging	Het
Vmn2r-ps158	T	A	7: 42,672,684 (GRCm39)	H106Q	probably benign	Het
Wscd1	G	T	11: 71,659,501 (GRCm39)	G194W	possibly damaging	Het
Zfp354b	A	C	11: 50,813,917 (GRCm39)	I336S	probably benign	Het
Zfp462	T	A	4: 55,016,887 (GRCm39)	V870E	possibly damaging	Het

Other mutations in Or2d3c

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01626:Or2d3c	APN	7	106,526,627 (GRCm39)	missense	probably benign	0.12
IGL02111:Or2d3c	APN	7	106,525,823 (GRCm39)	missense	probably damaging	0.99
IGL02891:Or2d3c	APN	7	106,526,288 (GRCm39)	missense	probably damaging	0.99
PIT4453001:Or2d3c	UTSW	7	106,526,049 (GRCm39)	missense	probably benign	0.07
R1553:Or2d3c	UTSW	7	106,526,201 (GRCm39)	missense	possibly damaging	0.78
R1701:Or2d3c	UTSW	7	106,526,129 (GRCm39)	missense	probably damaging	1.00
R2065:Or2d3c	UTSW	7	106,526,162 (GRCm39)	missense	probably damaging	1.00
R2068:Or2d3c	UTSW	7	106,526,162 (GRCm39)	missense	probably damaging	1.00
R2167:Or2d3c	UTSW	7	106,525,797 (GRCm39)	missense	probably damaging	1.00
R3683:Or2d3c	UTSW	7	106,526,301 (GRCm39)	nonsense	probably null
R4640:Or2d3c	UTSW	7	106,525,800 (GRCm39)	missense	possibly damaging	0.73
R5153:Or2d3c	UTSW	7	106,525,776 (GRCm39)	missense	possibly damaging	0.91
R5591:Or2d3c	UTSW	7	106,526,489 (GRCm39)	missense	probably damaging	1.00
R6052:Or2d3c	UTSW	7	106,525,896 (GRCm39)	missense	probably benign	0.05
R7358:Or2d3c	UTSW	7	106,526,111 (GRCm39)	missense	probably damaging	1.00
R7513:Or2d3c	UTSW	7	106,526,483 (GRCm39)	missense	possibly damaging	0.64
R7720:Or2d3c	UTSW	7	106,526,618 (GRCm39)	missense	probably benign	0.27
R7860:Or2d3c	UTSW	7	106,525,777 (GRCm39)	missense	probably benign	0.06
R8814:Or2d3c	UTSW	7	106,526,025 (GRCm39)	missense	probably damaging	1.00
R8989:Or2d3c	UTSW	7	106,526,366 (GRCm39)	missense	probably damaging	1.00
R9342:Or2d3c	UTSW	7	106,526,564 (GRCm39)	missense	possibly damaging	0.90
R9564:Or2d3c	UTSW	7	106,525,847 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- GCTAGGAGGATTACCACACC -3'
(R):5'- TTCTACTACCACAGTGCCCCAG -3'

Sequencing Primer
(F):5'- GCTAGGAGGATTACCACACCCATTG -3'
(R):5'- AGGTGCTTGTCCACTTCCTGG -3'

Posted On

2016-07-22