Mutagenetix > Incidental Mutation

Incidental Mutation 'R5223:Oprk1'

402397

Institutional Source

Beutler Lab

Gene Symbol

Oprk1

Ensembl Gene

ENSMUSG00000025905

Gene Name

opioid receptor, kappa 1

Synonyms

R21, KOR-1, Oprk2

MMRRC Submission

042796-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.166) question?

Stock #

R5223 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

5588466-5606131 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 5589296 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 83 (V83A)

Ref Sequence

ENSEMBL: ENSMUSP00000125105 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000027038] [ENSMUST00000160339] [ENSMUST00000160777]

AlphaFold

P33534

Predicted Effect

probably benign
Transcript: ENSMUST00000027038
AA Change: V83A

PolyPhen 2 Score 0.304 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000027038
Gene: ENSMUSG00000025905
AA Change: V83A

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	67	280	4.8e-8	PFAM
Pfam:7TM_GPCR_Srsx	70	345	5.4e-14	PFAM
Pfam:7tm_1	76	330	2.6e-67	PFAM
Pfam:7TM_GPCR_Srv	79	345	6.8e-9	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000160339
AA Change: V83A

PolyPhen 2 Score 0.304 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000124030
Gene: ENSMUSG00000025905
AA Change: V83A

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	67	284	1.1e-7	PFAM
Pfam:7TM_GPCR_Srsx	70	345	5.4e-14	PFAM
Pfam:7tm_1	76	330	2.6e-60	PFAM
Pfam:7TM_GPCR_Srv	78	345	5.2e-9	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000160777
AA Change: V83A

PolyPhen 2 Score 0.304 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000125105
Gene: ENSMUSG00000025905
AA Change: V83A

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	67	280	4.8e-8	PFAM
Pfam:7TM_GPCR_Srsx	70	345	5.4e-14	PFAM
Pfam:7tm_1	76	330	2.6e-67	PFAM
Pfam:7TM_GPCR_Srv	79	345	6.8e-9	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000192685

Coding Region Coverage

1x: 99.2%
3x: 98.4%
10x: 96.6%
20x: 93.2%

Validation Efficiency

MGI Phenotype

FUNCTION: This gene encodes an opioid receptor, which is a member of the 7 transmembrane-spanning G protein-coupled receptor family. It functions as a receptor for endogenous ligands, as well as a receptor for various synthetic opioids. Ligand binding results in inhibition of adenylate cyclase activity and neurotransmitter release. This opioid receptor plays a role in the perception of pain and mediating the hypolocomotor, analgesic and aversive actions of synthetic opioids. Variations in this gene have also been associated with alcohol dependence and opiate addiction. Alternatively spliced transcript variants have been found for this gene. A recent study provided evidence for translational readthrough in this gene and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Jan 2016]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit impaired response to morphine and an opioid agonist, abnormal pain threshold, and increased litter size. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 70 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aatk	C	T	11: 120,013,452	V273M	possibly damaging	Het
Acin1	CCGC	CC	14: 54,642,941		probably null	Het
Acvr1b	T	A	15: 101,193,976	C46S	probably damaging	Het
Ahi1	A	T	10: 20,970,919	H416L	possibly damaging	Het
Aspm	T	A	1: 139,478,334	L1653Q	probably damaging	Het
Cacna1a	G	A	8: 84,587,195	V1533M	possibly damaging	Het
Ccdc33	C	T	9: 58,032,984	E502K	possibly damaging	Het
Ctnnd1	C	T	2: 84,616,789	V371M	probably damaging	Het
Cyp2a12	A	T	7: 27,036,463		probably null	Het
Ep400	A	G	5: 110,668,630	V2675A	probably damaging	Het
Foxh1	T	A	15: 76,668,729		probably null	Het
Gad1-ps	G	A	10: 99,445,147		noncoding transcript	Het
Gm10093	A	G	17: 78,492,438	E286G	probably benign	Het
Gm13078	T	A	4: 143,728,021	S296R	probably benign	Het
Gpnmb	C	T	6: 49,056,205	T539M	probably benign	Het
Hspa9	A	G	18: 34,952,671		probably null	Het
Hspg2	T	C	4: 137,543,914	L2454P	probably damaging	Het
Ift140	T	A	17: 25,035,812	I422N	probably benign	Het
Igkv6-32	T	C	6: 70,074,223	S50G	probably benign	Het
Il23r	T	A	6: 67,486,170	Y113F	probably benign	Het
Kcnt1	A	G	2: 25,903,422	D636G	probably benign	Het
Klhl41	G	A	2: 69,679,827	W569*	probably null	Het
Klra4	T	A	6: 130,062,147	D94V	probably damaging	Het
Lca5	T	A	9: 83,398,613	H378L	probably benign	Het
Lhx8	T	A	3: 154,321,644	T254S	probably damaging	Het
Lrch3	C	T	16: 32,914,397	R86W	probably damaging	Het
Lrp2	T	A	2: 69,524,053	N477I	probably damaging	Het
Man2a1	T	A	17: 64,712,271	I710K	probably benign	Het
Ncor1	A	T	11: 62,339,000	Y881N	probably damaging	Het
Nhsl1	T	A	10: 18,526,326	V1100E	probably damaging	Het
Olfr1208	T	C	2: 88,897,334	T88A	probably benign	Het
Olfr486	A	G	7: 108,172,708	V12A	probably benign	Het
Olfr855	T	C	9: 19,585,026	V163A	probably benign	Het
Pacs1	C	T	19: 5,145,141	V472I	probably benign	Het
Pard3b	T	C	1: 62,344,113	Y789H	probably damaging	Het
Pcdha2	T	C	18: 36,940,791	Y492H	probably damaging	Het
Pcnt	T	C	10: 76,380,272	N2261D	probably damaging	Het
Pex5l	A	T	3: 32,958,796	S15T	probably damaging	Het
Plekhg4	A	G	8: 105,378,949	N682S	probably benign	Het
Poc5	A	T	13: 96,402,955	M335L	probably benign	Het
Polr1a	C	T	6: 71,967,907	R1316W	possibly damaging	Het
Pp2d1	T	C	17: 53,507,845	H617R	probably benign	Het
Prpsap1	T	C	11: 116,488,148	K65E	probably benign	Het
Ptgfrn	T	C	3: 101,045,593	E775G	probably benign	Het
Ptprc	T	A	1: 138,117,862	I87F	probably benign	Het
Rbbp8	C	A	18: 11,721,690	A324E	probably benign	Het
Rfx6	G	T	10: 51,677,996	G63*	probably null	Het
Rpl37a	T	C	1: 72,712,149	M47T	probably benign	Het
Samm50	T	G	15: 84,200,630	N187K	probably benign	Het
Skiv2l	A	G	17: 34,845,166		probably null	Het
Slamf9	T	C	1: 172,476,232	I48T	possibly damaging	Het
Slc2a12	A	G	10: 22,702,032	K576E	probably damaging	Het
Slc4a10	G	A	2: 62,253,366	G388S	probably damaging	Het
Smtn	G	T	11: 3,529,530	N512K	probably benign	Het
Sntb1	C	G	15: 55,642,795	G461R	probably damaging	Het
Sspo	A	G	6: 48,478,324	Y3040C	probably damaging	Het
Stat1	T	A	1: 52,144,242	V389E	probably damaging	Het
Sult5a1	A	T	8: 123,145,422	M227K	probably damaging	Het
Thsd4	T	C	9: 60,057,042	D389G	probably damaging	Het
Tnxb	T	C	17: 34,704,078	V2545A	possibly damaging	Het
Tpo	T	A	12: 30,092,590	I712F	probably damaging	Het
Trim62	T	C	4: 128,909,411	V418A	probably damaging	Het
Uqcrc1	C	A	9: 108,942,156	H95N	probably damaging	Het
Vmn2r114	ATTT	ATT	17: 23,290,932		probably null	Het
Vmn2r66	T	C	7: 85,007,885	D104G	probably benign	Het
Wdr26	T	C	1: 181,187,686	I371V	probably benign	Het
Wdr78	T	A	4: 103,049,403	S738C	possibly damaging	Het
Zfp273	T	G	13: 67,826,179	C475W	probably damaging	Het
Zfp738	G	T	13: 67,673,063	T55K	probably damaging	Het
Zmym2	A	G	14: 56,946,514	I978V	probably benign	Het

Other mutations in Oprk1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00926:Oprk1	APN	1	5598905	missense	probably damaging	0.99
IGL02049:Oprk1	APN	1	5598844	missense	probably damaging	1.00
IGL02076:Oprk1	APN	1	5602289	missense	probably damaging	1.00
IGL02265:Oprk1	APN	1	5602648	missense	probably damaging	1.00
IGL02294:Oprk1	APN	1	5602387	missense	probably damaging	1.00
IGL02584:Oprk1	APN	1	5598604	missense	probably damaging	1.00
IGL03164:Oprk1	APN	1	5598864	missense	probably damaging	1.00
R0295:Oprk1	UTSW	1	5598850	missense	possibly damaging	0.78
R1209:Oprk1	UTSW	1	5602261	missense	probably benign	0.00
R1420:Oprk1	UTSW	1	5602321	missense	probably damaging	1.00
R2994:Oprk1	UTSW	1	5602732	missense	probably benign	0.00
R3876:Oprk1	UTSW	1	5602661	nonsense	probably null
R4026:Oprk1	UTSW	1	5598685	missense	probably benign	0.04
R4096:Oprk1	UTSW	1	5602811	utr 3 prime	probably benign
R4097:Oprk1	UTSW	1	5602811	utr 3 prime	probably benign
R4475:Oprk1	UTSW	1	5602601	nonsense	probably null
R5177:Oprk1	UTSW	1	5602674	missense	probably damaging	1.00
R6397:Oprk1	UTSW	1	5598748	missense	probably damaging	1.00
R6647:Oprk1	UTSW	1	5602284	missense	probably damaging	1.00
R7169:Oprk1	UTSW	1	5589081	missense	probably benign
R7170:Oprk1	UTSW	1	5602396	missense	probably damaging	1.00
R8186:Oprk1	UTSW	1	5602317	missense	probably benign	0.16
R9712:Oprk1	UTSW	1	5598873	missense	probably damaging	0.98
Z1176:Oprk1	UTSW	1	5602702	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- AGATCTTCCGAGGAGATCCAG -3'
(R):5'- GATGTCACCTTGGAGAATCTCTC -3'

Sequencing Primer
(F):5'- AGGAGATCCAGGCCCTACC -3'
(R):5'- GCTGTCTGGTATACACAC -3'

Posted On

2016-07-22