Mutagenetix > Incidental Mutation

Incidental Mutation 'R0415:Pard3'

40246

Institutional Source

Beutler Lab

Gene Symbol

Pard3

Ensembl Gene

ENSMUSG00000025812

Gene Name

par-3 family cell polarity regulator

Synonyms

ASIP, PAR-3, Pard3a, Par3, D8Ertd580e

MMRRC Submission

038617-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R0415 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

127063893-127612286 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 127610566 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Aspartic acid at position 1221 (G1221D)

Ref Sequence

ENSEMBL: ENSMUSP00000125212 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000026921] [ENSMUST00000160272] [ENSMUST00000160766] [ENSMUST00000162309] [ENSMUST00000162536]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000026921
AA Change: G1251D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000026921
Gene: ENSMUSG00000025812
AA Change: G1251D

Domain	Start	End	E-Value	Type
Pfam:DUF3534	1	146	1.1e-72	PFAM
low complexity region	234	246	N/A	INTRINSIC
PDZ	282	361	2.34e-6	SMART
low complexity region	431	440	N/A	INTRINSIC
PDZ	469	548	4.1e-20	SMART
PDZ	599	684	9.87e-14	SMART
low complexity region	771	781	N/A	INTRINSIC
PDB:4DC2\|Z	810	837	3e-10	PDB
low complexity region	863	875	N/A	INTRINSIC
low complexity region	892	902	N/A	INTRINSIC
low complexity region	921	950	N/A	INTRINSIC
low complexity region	965	1005	N/A	INTRINSIC
low complexity region	1162	1200	N/A	INTRINSIC
low complexity region	1264	1281	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000159940

Predicted Effect

probably damaging
Transcript: ENSMUST00000160272
AA Change: G1266D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000125453
Gene: ENSMUSG00000025812
AA Change: G1266D

Domain	Start	End	E-Value	Type
Pfam:DUF3534	1	146	1.7e-60	PFAM
low complexity region	234	246	N/A	INTRINSIC
PDZ	282	361	2.34e-6	SMART
low complexity region	431	440	N/A	INTRINSIC
PDZ	469	548	4.1e-20	SMART
PDZ	599	684	9.87e-14	SMART
low complexity region	771	781	N/A	INTRINSIC
PDB:4DC2\|Z	810	837	4e-10	PDB
low complexity region	878	890	N/A	INTRINSIC
low complexity region	907	917	N/A	INTRINSIC
low complexity region	936	965	N/A	INTRINSIC
low complexity region	980	1020	N/A	INTRINSIC
low complexity region	1177	1215	N/A	INTRINSIC
low complexity region	1279	1296	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000160766
AA Change: G1179D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000124533
Gene: ENSMUSG00000025812
AA Change: G1179D

Domain	Start	End	E-Value	Type
Pfam:DUF3534	1	146	1e-72	PFAM
PDZ	238	317	2.34e-6	SMART
low complexity region	387	396	N/A	INTRINSIC
PDZ	425	504	4.1e-20	SMART
PDZ	542	627	9.87e-14	SMART
low complexity region	714	724	N/A	INTRINSIC
low complexity region	791	803	N/A	INTRINSIC
low complexity region	820	830	N/A	INTRINSIC
low complexity region	849	878	N/A	INTRINSIC
low complexity region	893	933	N/A	INTRINSIC
low complexity region	1090	1128	N/A	INTRINSIC
low complexity region	1192	1209	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000162309
AA Change: G1265D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000124282
Gene: ENSMUSG00000025812
AA Change: G1265D

Domain	Start	End	E-Value	Type
Pfam:DUF3534	1	146	6.2e-73	PFAM
low complexity region	234	246	N/A	INTRINSIC
PDZ	282	361	2.34e-6	SMART
low complexity region	431	440	N/A	INTRINSIC
PDZ	469	548	4.1e-20	SMART
PDZ	599	684	9.87e-14	SMART
low complexity region	771	781	N/A	INTRINSIC
PDB:4DC2\|Z	810	837	4e-10	PDB
low complexity region	877	889	N/A	INTRINSIC
low complexity region	906	916	N/A	INTRINSIC
low complexity region	935	964	N/A	INTRINSIC
low complexity region	979	1019	N/A	INTRINSIC
low complexity region	1176	1214	N/A	INTRINSIC
low complexity region	1278	1295	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000162536
AA Change: G1221D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000125212
Gene: ENSMUSG00000025812
AA Change: G1221D

Domain	Start	End	E-Value	Type
Pfam:DUF3534	1	146	1e-72	PFAM
PDZ	238	317	2.34e-6	SMART
low complexity region	387	396	N/A	INTRINSIC
PDZ	425	504	4.1e-20	SMART
PDZ	555	640	9.87e-14	SMART
low complexity region	727	737	N/A	INTRINSIC
PDB:4DC2\|Z	766	793	3e-10	PDB
low complexity region	833	845	N/A	INTRINSIC
low complexity region	862	872	N/A	INTRINSIC
low complexity region	891	920	N/A	INTRINSIC
low complexity region	935	975	N/A	INTRINSIC
low complexity region	1132	1170	N/A	INTRINSIC
low complexity region	1234	1251	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000162665
AA Change: G1255D

SMART Domains

Protein: ENSMUSP00000124718
Gene: ENSMUSG00000025812
AA Change: G1255D

Domain	Start	End	E-Value	Type
Pfam:DUF3534	21	166	1.4e-60	PFAM
low complexity region	254	266	N/A	INTRINSIC
PDZ	302	381	2.34e-6	SMART
low complexity region	451	460	N/A	INTRINSIC
PDZ	489	568	4.1e-20	SMART
PDZ	619	704	9.87e-14	SMART
low complexity region	791	801	N/A	INTRINSIC
low complexity region	868	880	N/A	INTRINSIC
low complexity region	897	907	N/A	INTRINSIC
low complexity region	926	955	N/A	INTRINSIC
low complexity region	970	1010	N/A	INTRINSIC
low complexity region	1167	1205	N/A	INTRINSIC
low complexity region	1269	1286	N/A	INTRINSIC

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.7%
20x: 93.6%

Validation Efficiency

97% (99/102)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the PARD protein family. PARD family members interact with other PARD family members and other proteins; they affect asymmetrical cell division and direct polarized cell growth. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Oct 2011]
PHENOTYPE: Mice homozygous for a null allele exhibit embryonic lethality at E12.5 associated with growth retardation, abnormal heart development, and abnormal epicardial cell development. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 98 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933427G23Rik	A	T	5: 23,831,050 (GRCm38)		noncoding transcript	Het
Acox2	A	G	14: 8,243,835 (GRCm38)		probably benign	Het
Adgb	T	C	10: 10,431,067 (GRCm38)		probably null	Het
Adgra3	C	A	5: 49,961,757 (GRCm38)		probably benign	Het
Adgre4	G	A	17: 55,852,288 (GRCm38)	V658I	probably benign	Het
Ahnak	A	G	19: 9,012,871 (GRCm38)		probably benign	Het
Anapc2	A	G	2: 25,278,325 (GRCm38)	T159A	probably damaging	Het
Arfgef3	A	G	10: 18,613,127 (GRCm38)		probably benign	Het
Atf7ip	C	T	6: 136,560,012 (GRCm38)	S81L	possibly damaging	Het
Cacna1i	A	G	15: 80,368,830 (GRCm38)		probably benign	Het
Camk1	A	T	6: 113,341,891 (GRCm38)	Y20*	probably null	Het
Ccdc40	T	C	11: 119,232,118 (GRCm38)	Y249H	possibly damaging	Het
Cd109	T	A	9: 78,712,615 (GRCm38)	S1380T	probably benign	Het
Cfap57	A	T	4: 118,569,431 (GRCm38)	L1107Q	possibly damaging	Het
Col6a4	C	T	9: 106,075,080 (GRCm38)	V540I	probably damaging	Het
Cst9	T	A	2: 148,838,442 (GRCm38)		probably benign	Het
Cul5	C	T	9: 53,667,070 (GRCm38)	V73I	probably benign	Het
Cxcl16	T	A	11: 70,458,748 (GRCm38)	K84*	probably null	Het
Cyp2c29	T	C	19: 39,329,095 (GRCm38)		probably benign	Het
D6Ertd527e	C	G	6: 87,111,524 (GRCm38)	T223S	unknown	Het
Dip2c	C	T	13: 9,568,289 (GRCm38)		probably benign	Het
Dis3	A	T	14: 99,087,456 (GRCm38)	I513N	probably damaging	Het
Dnajc16	A	T	4: 141,789,048 (GRCm38)	L3*	probably null	Het
Dopey1	T	A	9: 86,506,502 (GRCm38)	L480M	probably damaging	Het
Eml6	A	G	11: 29,749,392 (GRCm38)	V1787A	possibly damaging	Het
Etnk1	A	G	6: 143,180,774 (GRCm38)	N115S	probably damaging	Het
Fryl	T	C	5: 73,098,414 (GRCm38)	Y758C	probably damaging	Het
Gbp4	G	A	5: 105,121,106 (GRCm38)	R394C	possibly damaging	Het
Ggnbp2	A	C	11: 84,833,225 (GRCm38)		probably benign	Het
Gm7137	A	G	10: 77,788,173 (GRCm38)		probably benign	Het
Gstm2	T	A	3: 107,984,006 (GRCm38)	Q132L	probably benign	Het
Habp2	T	C	19: 56,317,717 (GRCm38)		probably benign	Het
Hectd2	T	C	19: 36,584,884 (GRCm38)		probably benign	Het
Htr6	A	G	4: 139,062,081 (GRCm38)	I291T	possibly damaging	Het
Ighg2c	T	C	12: 113,287,910 (GRCm38)	D199G	unknown	Het
Itih2	A	G	2: 10,105,615 (GRCm38)		probably benign	Het
Kcnab2	A	G	4: 152,395,136 (GRCm38)	F248S	probably benign	Het
Kcnc4	T	C	3: 107,445,433 (GRCm38)	K610E	probably damaging	Het
Kcnk16	T	A	14: 20,262,975 (GRCm38)		probably null	Het
Kndc1	C	T	7: 139,930,124 (GRCm38)	T1293I	probably damaging	Het
Lcp1	A	T	14: 75,227,006 (GRCm38)	I556F	possibly damaging	Het
Lrrc8d	T	C	5: 105,811,865 (GRCm38)	L47P	probably damaging	Het
Lyst	T	C	13: 13,711,610 (GRCm38)		probably benign	Het
Macrod2	G	A	2: 142,210,145 (GRCm38)		probably null	Het
Micalcl	C	T	7: 112,381,028 (GRCm38)	R70C	probably damaging	Het
Mllt3	ACTGCTGCTGCTGCTGCTGCT	ACTGCTGCTGCTGCTGCT	4: 87,841,339 (GRCm38)		probably benign	Het
Msh3	A	G	13: 92,346,786 (GRCm38)	V283A	possibly damaging	Het
Nup205	T	C	6: 35,214,634 (GRCm38)		probably benign	Het
Nxpe2	T	C	9: 48,326,614 (GRCm38)	T114A	probably damaging	Het
Olfr1023	A	T	2: 85,887,438 (GRCm38)	I213F	possibly damaging	Het
Olfr1034	A	T	2: 86,047,055 (GRCm38)	H191L	probably benign	Het
Olfr229	A	G	9: 39,909,983 (GRCm38)	Y60C	probably damaging	Het
Olfr78	C	T	7: 102,742,087 (GRCm38)	M305I	probably benign	Het
Olfr893	A	T	9: 38,209,973 (GRCm38)	M305L	probably benign	Het
Pax5	G	A	4: 44,691,886 (GRCm38)	A120V	probably damaging	Het
Pcsk6	C	T	7: 66,033,874 (GRCm38)	R746C	probably damaging	Het
Pif1	G	A	9: 65,588,051 (GRCm38)	C81Y	probably benign	Het
Plcb1	A	G	2: 135,337,499 (GRCm38)	Y609C	probably damaging	Het
Plcd4	C	A	1: 74,552,097 (GRCm38)	S217Y	probably damaging	Het
Plxna1	G	A	6: 89,357,336 (GRCm38)	H104Y	probably benign	Het
Polr2k	A	G	15: 36,175,456 (GRCm38)	Y45C	probably damaging	Het
Pqlc3	C	A	12: 16,997,710 (GRCm38)		probably benign	Het
Prex1	A	G	2: 166,586,699 (GRCm38)		probably benign	Het
Pth2r	A	G	1: 65,388,439 (GRCm38)	M424V	probably benign	Het
Pygm	A	G	19: 6,391,366 (GRCm38)	R464G	probably benign	Het
Rad51c	A	G	11: 87,397,655 (GRCm38)	L234P	probably damaging	Het
Rnf145	A	G	11: 44,525,138 (GRCm38)	Y60C	probably damaging	Het
Rnf167	T	C	11: 70,649,699 (GRCm38)	I135T	probably damaging	Het
Rnf213	A	T	11: 119,414,469 (GRCm38)	I509F	probably damaging	Het
Ryr2	T	C	13: 11,869,156 (GRCm38)	S213G	probably damaging	Het
Selenbp1	T	C	3: 94,936,913 (GRCm38)	V27A	possibly damaging	Het
Selenof	T	G	3: 144,577,692 (GRCm38)	L14R	probably damaging	Het
Sfswap	A	T	5: 129,504,126 (GRCm38)	D121V	probably damaging	Het
Slc25a34	C	A	4: 141,620,469 (GRCm38)	M300I	possibly damaging	Het
Slc34a3	T	G	2: 25,229,110 (GRCm38)	T583P	probably benign	Het
Smg1	C	A	7: 118,182,468 (GRCm38)	A1199S	probably benign	Het
Spint1	A	G	2: 119,245,615 (GRCm38)	T231A	probably damaging	Het
Sptbn1	A	C	11: 30,149,576 (GRCm38)	N229K	probably damaging	Het
Sult2b1	A	G	7: 45,730,092 (GRCm38)		probably benign	Het
Tas2r123	A	T	6: 132,847,838 (GRCm38)	M233L	probably damaging	Het
Tbcel	C	A	9: 42,444,500 (GRCm38)	C139F	probably benign	Het
Thbs2	A	C	17: 14,679,973 (GRCm38)	S573A	probably benign	Het
Tmem132c	A	G	5: 127,563,705 (GRCm38)	E980G	probably damaging	Het
Tmem247	G	T	17: 86,922,322 (GRCm38)	C197F	probably damaging	Het
Tmem251	T	A	12: 102,744,876 (GRCm38)	Y119*	probably null	Het
Tmem43	C	A	6: 91,482,318 (GRCm38)	P257Q	probably benign	Het
Tmprss13	A	G	9: 45,337,132 (GRCm38)		probably null	Het
Trove2	G	T	1: 143,760,075 (GRCm38)	N444K	probably benign	Het
Ubr5	T	C	15: 37,972,980 (GRCm38)	T2626A	probably damaging	Het
Vmn1r196	T	A	13: 22,293,836 (GRCm38)	V215D	probably damaging	Het
Vmn1r22	G	T	6: 57,900,332 (GRCm38)	T220K	probably benign	Het
Vmn2r116	G	A	17: 23,387,279 (GRCm38)	M388I	possibly damaging	Het
Vmn2r74	A	C	7: 85,961,410 (GRCm38)	C25G	probably damaging	Het
Xndc1	T	C	7: 102,080,616 (GRCm38)		probably benign	Het
Zfp282	A	G	6: 47,897,881 (GRCm38)	D340G	probably damaging	Het
Zfp282	T	A	6: 47,905,053 (GRCm38)	I558N	possibly damaging	Het
Zfp316	T	A	5: 143,264,491 (GRCm38)	T56S	unknown	Het
Zfp345	A	G	2: 150,474,559 (GRCm38)		probably benign	Het

Other mutations in Pard3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00161:Pard3	APN	8	127,359,818 (GRCm38)	splice site	probably benign
IGL00484:Pard3	APN	8	127,371,846 (GRCm38)	missense	probably benign	0.05
IGL00674:Pard3	APN	8	127,388,678 (GRCm38)	missense	probably damaging	1.00
IGL01471:Pard3	APN	8	127,378,246 (GRCm38)	missense	probably benign	0.01
IGL01505:Pard3	APN	8	127,324,063 (GRCm38)	missense	probably damaging	1.00
IGL02252:Pard3	APN	8	127,398,756 (GRCm38)	missense	probably benign	0.09
IGL02511:Pard3	APN	8	127,161,320 (GRCm38)	splice site	probably benign
IGL02838:Pard3	APN	8	127,426,647 (GRCm38)	missense	probably damaging	0.99
IGL02948:Pard3	APN	8	127,306,494 (GRCm38)	missense	probably benign	0.00
IGL02987:Pard3	APN	8	127,389,491 (GRCm38)	missense	probably damaging	0.98
IGL03037:Pard3	APN	8	127,306,494 (GRCm38)	missense	probably benign	0.00
IGL03084:Pard3	APN	8	127,593,092 (GRCm38)	missense	probably damaging	0.96
BB001:Pard3	UTSW	8	127,410,750 (GRCm38)	missense	probably benign
BB011:Pard3	UTSW	8	127,410,750 (GRCm38)	missense	probably benign
R0025:Pard3	UTSW	8	127,161,308 (GRCm38)	missense	probably damaging	1.00
R0025:Pard3	UTSW	8	127,161,308 (GRCm38)	missense	probably damaging	1.00
R0029:Pard3	UTSW	8	127,426,758 (GRCm38)	splice site	probably benign
R0109:Pard3	UTSW	8	127,398,666 (GRCm38)	missense	probably damaging	1.00
R0309:Pard3	UTSW	8	127,376,897 (GRCm38)	splice site	probably benign
R0507:Pard3	UTSW	8	127,371,486 (GRCm38)	splice site	probably benign
R1055:Pard3	UTSW	8	127,378,280 (GRCm38)	missense	probably benign	0.34
R1305:Pard3	UTSW	8	127,306,410 (GRCm38)	missense	possibly damaging	0.62
R1619:Pard3	UTSW	8	127,380,502 (GRCm38)	missense	probably benign	0.02
R1855:Pard3	UTSW	8	127,447,812 (GRCm38)	splice site	probably null
R2001:Pard3	UTSW	8	127,064,347 (GRCm38)	splice site	probably null
R2060:Pard3	UTSW	8	127,398,604 (GRCm38)	missense	probably benign	0.05
R2064:Pard3	UTSW	8	127,610,611 (GRCm38)	missense	probably damaging	1.00
R2113:Pard3	UTSW	8	127,388,537 (GRCm38)	missense	probably damaging	1.00
R2136:Pard3	UTSW	8	127,376,885 (GRCm38)	critical splice donor site	probably null
R2224:Pard3	UTSW	8	127,359,776 (GRCm38)	missense	probably damaging	1.00
R2252:Pard3	UTSW	8	127,610,599 (GRCm38)	missense	probably damaging	1.00
R3870:Pard3	UTSW	8	127,409,686 (GRCm38)	missense	probably damaging	1.00
R4154:Pard3	UTSW	8	127,474,396 (GRCm38)	missense	probably damaging	1.00
R4212:Pard3	UTSW	8	127,610,458 (GRCm38)	missense	probably benign	0.43
R4243:Pard3	UTSW	8	127,371,647 (GRCm38)	missense	probably benign	0.09
R4523:Pard3	UTSW	8	127,398,627 (GRCm38)	missense	probably benign	0.08
R4857:Pard3	UTSW	8	127,324,054 (GRCm38)	missense	probably damaging	0.98
R4876:Pard3	UTSW	8	127,561,469 (GRCm38)	intron	probably benign
R4877:Pard3	UTSW	8	127,388,537 (GRCm38)	missense	probably damaging	1.00
R5197:Pard3	UTSW	8	127,073,290 (GRCm38)	splice site	probably null
R5215:Pard3	UTSW	8	127,378,264 (GRCm38)	missense	probably damaging	1.00
R5279:Pard3	UTSW	8	127,460,386 (GRCm38)	critical splice donor site	probably null
R5349:Pard3	UTSW	8	127,415,743 (GRCm38)	missense	probably damaging	1.00
R5479:Pard3	UTSW	8	127,370,355 (GRCm38)	missense	probably damaging	1.00
R5514:Pard3	UTSW	8	127,426,605 (GRCm38)	missense	probably damaging	1.00
R5681:Pard3	UTSW	8	127,389,433 (GRCm38)	missense	possibly damaging	0.81
R5934:Pard3	UTSW	8	127,389,338 (GRCm38)	missense	probably damaging	1.00
R6034:Pard3	UTSW	8	127,064,327 (GRCm38)	utr 5 prime	probably benign
R6034:Pard3	UTSW	8	127,064,327 (GRCm38)	utr 5 prime	probably benign
R6187:Pard3	UTSW	8	127,073,273 (GRCm38)	missense	probably benign	0.00
R6382:Pard3	UTSW	8	127,376,783 (GRCm38)	missense	probably damaging	1.00
R6774:Pard3	UTSW	8	127,410,747 (GRCm38)	missense	probably damaging	0.98
R7130:Pard3	UTSW	8	127,415,683 (GRCm38)	missense	probably damaging	1.00
R7267:Pard3	UTSW	8	127,371,575 (GRCm38)	missense	probably damaging	0.97
R7358:Pard3	UTSW	8	127,593,092 (GRCm38)	missense	probably damaging	0.98
R7528:Pard3	UTSW	8	127,603,165 (GRCm38)	missense	probably damaging	1.00
R7537:Pard3	UTSW	8	127,610,582 (GRCm38)	missense	probably damaging	1.00
R7679:Pard3	UTSW	8	127,371,846 (GRCm38)	missense	probably benign	0.05
R7924:Pard3	UTSW	8	127,410,750 (GRCm38)	missense	probably benign
R8076:Pard3	UTSW	8	127,415,596 (GRCm38)	missense	probably damaging	1.00
R8258:Pard3	UTSW	8	127,371,540 (GRCm38)	nonsense	probably null
R8259:Pard3	UTSW	8	127,371,540 (GRCm38)	nonsense	probably null
R8345:Pard3	UTSW	8	127,324,068 (GRCm38)	missense	probably damaging	1.00
R8421:Pard3	UTSW	8	127,140,408 (GRCm38)	intron	probably benign
R8500:Pard3	UTSW	8	127,460,303 (GRCm38)	missense	probably damaging	1.00
R8742:Pard3	UTSW	8	127,324,111 (GRCm38)	missense	possibly damaging	0.85
R8918:Pard3	UTSW	8	127,371,530 (GRCm38)	missense	probably benign	0.29
R9005:Pard3	UTSW	8	127,277,166 (GRCm38)	missense	probably damaging	1.00
R9629:Pard3	UTSW	8	127,409,672 (GRCm38)	missense	possibly damaging	0.94
R9643:Pard3	UTSW	8	127,389,419 (GRCm38)	missense	possibly damaging	0.87

Predicted Primers

PCR Primer
(F):5'- AGCCCCTCACCATGATACCTGG -3'
(R):5'- CTTTGCTCATGCTGCTTAAAGGCAC -3'

Sequencing Primer
(F):5'- TGCTAAAGCCTGGAGATTCATC -3'
(R):5'- TCTGTTCCTGTCGGAGAAGCT -3'

Posted On

2013-05-23