Mutagenetix > Incidental Mutation

Incidental Mutation 'R5210:Adgre4'

403111

Institutional Source

Beutler Lab

Gene Symbol

Adgre4

Ensembl Gene

ENSMUSG00000032915

Gene Name

adhesion G protein-coupled receptor E4

Synonyms

EGF-TM7, Gpr127, FIRE, Emr4, D17Ertd479e

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5210 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

56056984-56160662 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 56092029 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Lysine at position 96 (N96K)

Ref Sequence

ENSEMBL: ENSMUSP00000025004 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000025004]

AlphaFold

Q91ZE5

Predicted Effect

probably damaging
Transcript: ENSMUST00000025004
AA Change: N96K

PolyPhen 2 Score 0.967 (Sensitivity: 0.77; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000025004
Gene: ENSMUSG00000032915
AA Change: N96K

Domain	Start	End	E-Value	Type
signal peptide	1	36	N/A	INTRINSIC
Blast:EGF_like	38	76	2e-10	BLAST
Pfam:EGF_CA	77	117	3.6e-9	PFAM
GPS	288	338	4.03e-12	SMART
Pfam:7tm_2	343	588	5.7e-57	PFAM
low complexity region	613	628	N/A	INTRINSIC

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.5%
20x: 95.8%

Validation Efficiency

98% (40/41)

Allele List at MGI

Other mutations in this stock

Total: 35 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ablim2	T	A	5: 35,994,416 (GRCm39)	V342E	probably benign	Het
Aopep	A	G	13: 63,215,924 (GRCm39)	I399V	probably benign	Het
Bltp1	T	C	3: 37,087,414 (GRCm39)	V710A	possibly damaging	Het
Ccdc171	A	G	4: 83,473,093 (GRCm39)	E174G	probably damaging	Het
Col11a1	T	C	3: 113,946,806 (GRCm39)	F1026S	probably damaging	Het
Coro1c	A	G	5: 113,983,367 (GRCm39)	L387P	probably damaging	Het
Dsg1c	C	T	18: 20,407,758 (GRCm39)	T368I	probably damaging	Het
Exoc3l4	C	A	12: 111,395,275 (GRCm39)		probably benign	Het
Extl1	T	C	4: 134,087,895 (GRCm39)	D453G	probably benign	Het
Gm21136	T	C	7: 38,567,165 (GRCm39)		noncoding transcript	Het
H3c4	G	T	13: 23,760,015 (GRCm39)	G14C	possibly damaging	Het
Ifi208	T	C	1: 173,510,831 (GRCm39)	S329P	probably benign	Het
Iscu	T	A	5: 113,915,034 (GRCm39)	L182*	probably null	Het
Klra6	T	A	6: 129,995,855 (GRCm39)	K168*	probably null	Het
Map3k5	T	C	10: 19,900,647 (GRCm39)	S274P	possibly damaging	Het
Megf6	G	T	4: 154,354,273 (GRCm39)		probably benign	Het
Mmp12	T	A	9: 7,349,729 (GRCm39)	Y53*	probably null	Het
Nucb2	T	A	7: 116,128,222 (GRCm39)	Y278N	probably damaging	Het
Numa1	C	A	7: 101,649,188 (GRCm39)	A973E	probably benign	Het
Or1o4	C	A	17: 37,590,824 (GRCm39)	K162N	probably benign	Het
Or2w1b	A	T	13: 21,300,222 (GRCm39)	Y120F	probably damaging	Het
Or5b123	A	G	19: 13,596,763 (GRCm39)	K79R	probably damaging	Het
Or7g18	T	A	9: 18,787,103 (GRCm39)	L157Q	probably damaging	Het
Pcdhga3	C	A	18: 37,808,963 (GRCm39)	T472K	probably benign	Het
Pclo	A	G	5: 14,763,464 (GRCm39)	D694G	probably damaging	Het
Plch1	A	G	3: 63,607,199 (GRCm39)		probably null	Het
Pou3f2	C	T	4: 22,487,324 (GRCm39)	D270N	probably damaging	Het
Prrc2a	G	A	17: 35,372,596 (GRCm39)	R1682W	probably damaging	Het
Rb1	A	G	14: 73,436,751 (GRCm39)	F838S	probably damaging	Het
Sec24c	A	G	14: 20,741,872 (GRCm39)	E769G	probably damaging	Het
Slc45a2	C	T	15: 11,027,871 (GRCm39)	T480I	probably damaging	Het
Tmem63c	A	T	12: 87,136,172 (GRCm39)	E796V	probably benign	Het
Tomm70a	G	A	16: 56,953,614 (GRCm39)		probably null	Het
Tpcn1	G	A	5: 120,677,279 (GRCm39)	T676I	probably damaging	Het
Vps51	T	G	19: 6,121,063 (GRCm39)	E283D	probably benign	Het

Other mutations in Adgre4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00089:Adgre4	APN	17	56,098,915 (GRCm39)	splice site	probably benign
IGL00228:Adgre4	APN	17	56,109,135 (GRCm39)	missense	probably damaging	1.00
IGL00572:Adgre4	APN	17	56,127,648 (GRCm39)	missense	probably benign	0.00
IGL01404:Adgre4	APN	17	56,104,639 (GRCm39)	missense	possibly damaging	0.63
IGL01420:Adgre4	APN	17	56,106,785 (GRCm39)	splice site	probably benign
IGL01501:Adgre4	APN	17	56,109,002 (GRCm39)	splice site	probably benign
IGL01510:Adgre4	APN	17	56,125,760 (GRCm39)	critical splice donor site	probably null
IGL01554:Adgre4	APN	17	56,124,090 (GRCm39)	missense	probably damaging	1.00
IGL01607:Adgre4	APN	17	56,101,748 (GRCm39)	splice site	probably benign
IGL01767:Adgre4	APN	17	56,104,740 (GRCm39)	missense	probably benign	0.19
IGL02253:Adgre4	APN	17	56,067,573 (GRCm39)	missense	probably benign	0.01
IGL02358:Adgre4	APN	17	56,150,209 (GRCm39)	missense	probably benign	0.15
IGL02466:Adgre4	APN	17	56,121,188 (GRCm39)	missense	probably benign	0.42
IGL03057:Adgre4	APN	17	56,106,602 (GRCm39)	splice site	probably benign
R0070:Adgre4	UTSW	17	56,109,154 (GRCm39)	missense	probably damaging	0.98
R0070:Adgre4	UTSW	17	56,109,154 (GRCm39)	missense	probably damaging	0.98
R0111:Adgre4	UTSW	17	56,124,073 (GRCm39)	missense	possibly damaging	0.92
R0311:Adgre4	UTSW	17	56,109,010 (GRCm39)	missense	probably benign	0.36
R0366:Adgre4	UTSW	17	56,099,001 (GRCm39)	nonsense	probably null
R0415:Adgre4	UTSW	17	56,159,288 (GRCm39)	missense	probably benign	0.03
R0465:Adgre4	UTSW	17	56,092,137 (GRCm39)	splice site	probably benign
R0619:Adgre4	UTSW	17	56,127,679 (GRCm39)	missense	possibly damaging	0.52
R0685:Adgre4	UTSW	17	56,099,035 (GRCm39)	missense	probably benign	0.05
R0724:Adgre4	UTSW	17	56,159,281 (GRCm39)	missense	probably benign	0.00
R0835:Adgre4	UTSW	17	56,106,637 (GRCm39)	missense	probably damaging	1.00
R1330:Adgre4	UTSW	17	56,085,814 (GRCm39)	missense	probably benign	0.36
R1452:Adgre4	UTSW	17	56,091,996 (GRCm39)	missense	probably benign	0.35
R1960:Adgre4	UTSW	17	56,098,497 (GRCm39)	missense	probably benign
R1961:Adgre4	UTSW	17	56,098,497 (GRCm39)	missense	probably benign
R2046:Adgre4	UTSW	17	56,085,847 (GRCm39)	missense	possibly damaging	0.82
R2421:Adgre4	UTSW	17	56,085,872 (GRCm39)	missense	probably benign	0.10
R2570:Adgre4	UTSW	17	56,085,878 (GRCm39)	missense	possibly damaging	0.54
R3162:Adgre4	UTSW	17	56,109,218 (GRCm39)	splice site	probably benign
R4222:Adgre4	UTSW	17	56,092,121 (GRCm39)	missense	probably damaging	1.00
R4526:Adgre4	UTSW	17	56,092,016 (GRCm39)	nonsense	probably null
R4631:Adgre4	UTSW	17	56,121,305 (GRCm39)	missense	probably null	1.00
R4689:Adgre4	UTSW	17	56,109,096 (GRCm39)	missense	probably damaging	1.00
R4701:Adgre4	UTSW	17	56,091,971 (GRCm39)	missense	probably damaging	1.00
R4792:Adgre4	UTSW	17	56,098,491 (GRCm39)	missense	probably benign	0.00
R5205:Adgre4	UTSW	17	56,101,727 (GRCm39)	nonsense	probably null
R5358:Adgre4	UTSW	17	56,125,758 (GRCm39)	missense	probably benign	0.00
R5873:Adgre4	UTSW	17	56,159,282 (GRCm39)	missense	probably benign	0.13
R6025:Adgre4	UTSW	17	56,099,013 (GRCm39)	missense	probably benign	0.00
R6257:Adgre4	UTSW	17	56,109,133 (GRCm39)	missense	possibly damaging	0.87
R6426:Adgre4	UTSW	17	56,109,196 (GRCm39)	missense	probably benign	0.18
R6440:Adgre4	UTSW	17	56,101,744 (GRCm39)	critical splice donor site	probably null
R6484:Adgre4	UTSW	17	56,109,036 (GRCm39)	missense	possibly damaging	0.52
R6680:Adgre4	UTSW	17	56,098,959 (GRCm39)	missense	probably benign	0.09
R7086:Adgre4	UTSW	17	56,127,649 (GRCm39)	missense	probably benign	0.00
R7442:Adgre4	UTSW	17	56,159,340 (GRCm39)	missense	probably benign	0.04
R7467:Adgre4	UTSW	17	56,098,952 (GRCm39)	missense	probably benign	0.00
R7875:Adgre4	UTSW	17	56,099,016 (GRCm39)	missense	probably benign	0.00
R8007:Adgre4	UTSW	17	56,121,233 (GRCm39)	missense	probably damaging	0.99
R8096:Adgre4	UTSW	17	56,127,700 (GRCm39)	missense	probably damaging	1.00
R8172:Adgre4	UTSW	17	56,104,769 (GRCm39)	missense	probably benign	0.00
R8512:Adgre4	UTSW	17	56,125,760 (GRCm39)	critical splice donor site	probably null
R8972:Adgre4	UTSW	17	56,109,189 (GRCm39)	missense	probably damaging	1.00
R9018:Adgre4	UTSW	17	56,098,993 (GRCm39)	missense	probably benign	0.00
R9049:Adgre4	UTSW	17	56,092,094 (GRCm39)	missense	probably benign	0.05
S24628:Adgre4	UTSW	17	56,159,288 (GRCm39)	missense	probably benign	0.03
X0010:Adgre4	UTSW	17	56,121,308 (GRCm39)	missense	probably damaging	1.00
Z1177:Adgre4	UTSW	17	56,121,152 (GRCm39)	critical splice acceptor site	probably null

Predicted Primers

PCR Primer
(F):5'- GATACATACTAAAGTGCTTGGAGAC -3'
(R):5'- CTAGCAGTGTTGTAGCTGTCAC -3'

Sequencing Primer
(F):5'- CTGGGAGTAGAGGCTTTATA -3'
(R):5'- AGTGTTGTAGCTGTCACAAGCTCTAC -3'

Posted On

2016-07-22