Incidental Mutation 'R5214:Zscan20'
ID 403328
Institutional Source Beutler Lab
Gene Symbol Zscan20
Ensembl Gene ENSMUSG00000061894
Gene Name zinc finger and SCAN domains 20
Synonyms Zfp31
MMRRC Submission 042787-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.223) question?
Stock # R5214 (G1)
Quality Score 225
Status Validated
Chromosome 4
Chromosomal Location 128477332-128503891 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) G to A at 128482109 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Arginine to Cysteine at position 518 (R518C)
Ref Sequence ENSEMBL: ENSMUSP00000095487 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000097877] [ENSMUST00000135309]
AlphaFold B2KFW1
Predicted Effect noncoding transcript
Transcript: ENSMUST00000084276
Predicted Effect probably benign
Transcript: ENSMUST00000097877
AA Change: R518C

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)
SMART Domains Protein: ENSMUSP00000095487
Gene: ENSMUSG00000061894
AA Change: R518C

DomainStartEndE-ValueType
SCAN 41 147 1.31e-54 SMART
low complexity region 275 284 N/A INTRINSIC
SANT 314 378 8.04e-1 SMART
SANT 475 539 4.85e-3 SMART
ZnF_C2H2 725 747 2.61e1 SMART
ZnF_C2H2 753 775 9.88e-5 SMART
ZnF_C2H2 781 803 1.12e-3 SMART
ZnF_C2H2 862 884 3.21e-4 SMART
ZnF_C2H2 890 912 1.04e-3 SMART
ZnF_C2H2 918 940 1.56e-2 SMART
ZnF_C2H2 946 968 2.84e-5 SMART
ZnF_C2H2 974 996 4.54e-4 SMART
ZnF_C2H2 1002 1024 1.47e-3 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000135309
SMART Domains Protein: ENSMUSP00000119338
Gene: ENSMUSG00000061894

DomainStartEndE-ValueType
SCAN 41 147 1.31e-54 SMART
low complexity region 279 293 N/A INTRINSIC
Meta Mutation Damage Score 0.0898 question?
Coding Region Coverage
  • 1x: 99.3%
  • 3x: 98.7%
  • 10x: 97.5%
  • 20x: 95.9%
Validation Efficiency 100% (77/77)
Allele List at MGI
Other mutations in this stock
Total: 69 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1700061I17Rik A G 3: 116,861,424 (GRCm39) noncoding transcript Het
4930449A18Rik A T 3: 59,733,305 (GRCm39) noncoding transcript Het
Acsbg3 T C 17: 57,193,493 (GRCm39) V613A probably benign Het
Adgrl1 G A 8: 84,642,202 (GRCm39) probably null Het
Aldh1l1 A G 6: 90,540,399 (GRCm39) D228G probably damaging Het
Ankrd17 T C 5: 90,431,319 (GRCm39) I822V possibly damaging Het
Atm C A 9: 53,402,327 (GRCm39) A1382S probably benign Het
Bltp3a G A 17: 28,106,489 (GRCm39) S1005N probably benign Het
Cacna1e T A 1: 154,577,110 (GRCm39) I96F possibly damaging Het
Calhm5 A G 10: 33,968,487 (GRCm39) S189P probably damaging Het
Ccar1 G A 10: 62,606,740 (GRCm39) R335C probably damaging Het
Ccdc113 T C 8: 96,272,601 (GRCm39) I236T possibly damaging Het
Ccdc14 T A 16: 34,525,225 (GRCm39) S125T probably benign Het
Cdc45 C T 16: 18,614,647 (GRCm39) R205H probably damaging Het
Cdon T C 9: 35,394,504 (GRCm39) C917R probably damaging Het
Ckap2l T C 2: 129,127,389 (GRCm39) D263G probably benign Het
Cntnap3 G T 13: 64,909,824 (GRCm39) H760Q probably damaging Het
Dennd2d G A 3: 106,393,637 (GRCm39) probably null Het
Dock4 A G 12: 40,754,465 (GRCm39) I485V probably benign Het
Dspp A G 5: 104,326,364 (GRCm39) D909G unknown Het
Eps8 A T 6: 137,504,490 (GRCm39) M81K probably damaging Het
Fras1 T C 5: 96,917,452 (GRCm39) S3491P probably damaging Het
Gm17669 C T 18: 67,695,479 (GRCm39) T8I possibly damaging Het
Gm5114 G T 7: 39,057,792 (GRCm39) T609K probably benign Het
Gm973 A G 1: 59,565,880 (GRCm39) N32S probably damaging Het
Herpud1 G T 8: 95,117,479 (GRCm39) probably null Het
Jcad T A 18: 4,674,134 (GRCm39) L632Q probably damaging Het
Kcnh8 T C 17: 53,205,486 (GRCm39) L527S probably damaging Het
Klk1b11 C A 7: 43,647,266 (GRCm39) H67N probably benign Het
Ldhb T A 6: 142,441,321 (GRCm39) I190F probably damaging Het
Lrrc34 A T 3: 30,690,397 (GRCm39) C168* probably null Het
Lrtm1 C T 14: 28,743,651 (GRCm39) H40Y possibly damaging Het
Mageb3 T C 2: 121,785,319 (GRCm39) M128V possibly damaging Het
Miga2 A G 2: 30,261,208 (GRCm39) T90A probably benign Het
Msantd5 C A 11: 51,125,675 (GRCm39) H199Q possibly damaging Het
Ndrg1 G A 15: 66,831,239 (GRCm39) T24I probably damaging Het
Nos2 T C 11: 78,846,267 (GRCm39) L878P probably damaging Het
Or11g27 T G 14: 50,771,804 (GRCm39) *312E probably null Het
Pcdhgb1 T A 18: 37,814,478 (GRCm39) I323N probably damaging Het
Plec A G 15: 76,061,921 (GRCm39) I2537T probably damaging Het
Pnpo C A 11: 96,833,295 (GRCm39) E68D probably benign Het
Ppp6r1 C T 7: 4,646,176 (GRCm39) R175Q probably benign Het
Prnp C T 2: 131,778,924 (GRCm39) T192I probably damaging Het
Ptprb T C 10: 116,205,229 (GRCm39) I2148T possibly damaging Het
Raf1 G T 6: 115,614,583 (GRCm39) F99L possibly damaging Het
Rbks A G 5: 31,807,736 (GRCm39) probably benign Het
Rlf T C 4: 121,007,897 (GRCm39) D361G probably damaging Het
Rnpepl1 G T 1: 92,847,001 (GRCm39) D608Y probably benign Het
Scaf1 G A 7: 44,652,662 (GRCm39) probably benign Het
Sh3rf1 A T 8: 61,825,765 (GRCm39) M587L probably damaging Het
Slc22a21 A T 11: 53,843,869 (GRCm39) S473T probably damaging Het
Syt15 A G 14: 33,943,703 (GRCm39) D84G possibly damaging Het
Tbc1d19 T C 5: 54,007,183 (GRCm39) L236P probably benign Het
Tbx2 G T 11: 85,729,263 (GRCm39) A549S probably benign Het
Tc2n A T 12: 101,659,461 (GRCm39) C157* probably null Het
Tecta C T 9: 42,256,964 (GRCm39) V1571I probably benign Het
Them4 A G 3: 94,224,818 (GRCm39) K65R probably benign Het
Tmc5 C T 7: 118,247,155 (GRCm39) T553M probably damaging Het
Tmem200c C T 17: 69,148,122 (GRCm39) A235V probably damaging Het
Tmem8b G A 4: 43,673,992 (GRCm39) V208I probably benign Het
Tmf1 G C 6: 97,144,253 (GRCm39) A701G possibly damaging Het
Tomm70a G A 16: 56,942,300 (GRCm39) G26S unknown Het
Treh T C 9: 44,594,173 (GRCm39) Y140H probably damaging Het
Ttc28 A G 5: 111,325,489 (GRCm39) probably benign Het
Uba7 T C 9: 107,854,713 (GRCm39) probably benign Het
Ube4a T C 9: 44,860,166 (GRCm39) I299V probably benign Het
Zfhx4 A C 3: 5,468,701 (GRCm39) K2953T probably damaging Het
Zfp280d T A 9: 72,215,395 (GRCm39) probably benign Het
Zw10 T C 9: 48,975,463 (GRCm39) I296T possibly damaging Het
Other mutations in Zscan20
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00552:Zscan20 APN 4 128,480,428 (GRCm39) missense probably damaging 0.99
IGL01454:Zscan20 APN 4 128,483,334 (GRCm39) missense probably benign 0.01
IGL01934:Zscan20 APN 4 128,486,277 (GRCm39) missense possibly damaging 0.94
IGL02288:Zscan20 APN 4 128,480,436 (GRCm39) missense probably damaging 1.00
IGL02336:Zscan20 APN 4 128,479,587 (GRCm39) missense probably damaging 1.00
IGL02385:Zscan20 APN 4 128,498,392 (GRCm39) missense possibly damaging 0.66
IGL02437:Zscan20 APN 4 128,482,210 (GRCm39) missense probably damaging 1.00
IGL02450:Zscan20 APN 4 128,480,450 (GRCm39) missense probably damaging 0.99
R0034:Zscan20 UTSW 4 128,479,455 (GRCm39) missense probably damaging 0.96
R0034:Zscan20 UTSW 4 128,479,455 (GRCm39) missense probably damaging 0.96
R0070:Zscan20 UTSW 4 128,479,675 (GRCm39) missense possibly damaging 0.73
R0142:Zscan20 UTSW 4 128,479,630 (GRCm39) missense probably benign 0.38
R0496:Zscan20 UTSW 4 128,485,682 (GRCm39) missense probably benign 0.42
R0567:Zscan20 UTSW 4 128,483,243 (GRCm39) critical splice donor site probably null
R1333:Zscan20 UTSW 4 128,481,889 (GRCm39) missense possibly damaging 0.61
R1716:Zscan20 UTSW 4 128,480,334 (GRCm39) missense probably damaging 0.96
R2302:Zscan20 UTSW 4 128,482,057 (GRCm39) missense probably damaging 1.00
R3870:Zscan20 UTSW 4 128,480,218 (GRCm39) missense probably damaging 1.00
R4611:Zscan20 UTSW 4 128,481,899 (GRCm39) missense probably benign 0.00
R4884:Zscan20 UTSW 4 128,481,958 (GRCm39) missense possibly damaging 0.56
R4972:Zscan20 UTSW 4 128,486,152 (GRCm39) missense probably benign 0.39
R5033:Zscan20 UTSW 4 128,479,921 (GRCm39) missense probably benign 0.01
R5160:Zscan20 UTSW 4 128,486,275 (GRCm39) missense possibly damaging 0.69
R5182:Zscan20 UTSW 4 128,480,504 (GRCm39) missense possibly damaging 0.71
R5345:Zscan20 UTSW 4 128,481,914 (GRCm39) missense probably benign 0.04
R5863:Zscan20 UTSW 4 128,480,141 (GRCm39) nonsense probably null
R6217:Zscan20 UTSW 4 128,498,327 (GRCm39) missense probably damaging 1.00
R6597:Zscan20 UTSW 4 128,479,539 (GRCm39) missense probably damaging 0.96
R6751:Zscan20 UTSW 4 128,479,668 (GRCm39) missense probably damaging 1.00
R6852:Zscan20 UTSW 4 128,483,515 (GRCm39) missense probably damaging 1.00
R7172:Zscan20 UTSW 4 128,479,469 (GRCm39) nonsense probably null
R7338:Zscan20 UTSW 4 128,481,943 (GRCm39) missense probably benign
R7805:Zscan20 UTSW 4 128,479,599 (GRCm39) missense probably damaging 1.00
R8063:Zscan20 UTSW 4 128,480,028 (GRCm39) missense probably benign 0.01
R8244:Zscan20 UTSW 4 128,479,759 (GRCm39) missense probably benign 0.32
R8421:Zscan20 UTSW 4 128,479,620 (GRCm39) missense probably damaging 1.00
R8752:Zscan20 UTSW 4 128,479,480 (GRCm39) missense probably damaging 1.00
R8939:Zscan20 UTSW 4 128,498,315 (GRCm39) missense probably benign 0.00
R8971:Zscan20 UTSW 4 128,479,848 (GRCm39) missense probably damaging 1.00
R8971:Zscan20 UTSW 4 128,479,847 (GRCm39) missense probably damaging 1.00
R9149:Zscan20 UTSW 4 128,481,914 (GRCm39) missense probably benign 0.15
R9458:Zscan20 UTSW 4 128,480,639 (GRCm39) critical splice acceptor site probably null
Predicted Primers PCR Primer
(F):5'- TCAGTACTGCTCTGCCCAGATC -3'
(R):5'- ACCCCAGTCCTAATGACCTAGG -3'

Sequencing Primer
(F):5'- AGATCCAGGGAGGCCTGTG -3'
(R):5'- GTCCTAATGACCTAGGTAAAGCTC -3'
Posted On 2016-07-22