Mutagenetix > Incidental Mutation

Incidental Mutation 'R5229:Apob'

403671

Institutional Source

Beutler Lab

Gene Symbol

Apob

Ensembl Gene

ENSMUSG00000020609

Gene Name

apolipoprotein B

Synonyms

apob-100, apob-48

MMRRC Submission

042802-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.859) question?

Stock #

R5229 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

8027648-8066835 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 8027806 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 10 (T10A)

Ref Sequence

ENSEMBL: ENSMUSP00000127147 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000037520] [ENSMUST00000037811] [ENSMUST00000171271]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000037520
AA Change: T10A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000035761
Gene: ENSMUSG00000020609
AA Change: T10A

Domain	Start	End	E-Value	Type
signal peptide	1	27	N/A	INTRINSIC
LPD_N	33	585	6.03e-94	SMART
DUF1943	619	932	7.88e-97	SMART
Pfam:DUF1081	945	1059	9.4e-32	PFAM
low complexity region	1100	1109	N/A	INTRINSIC
Blast:LPD_N	1249	1311	9e-22	BLAST
low complexity region	1632	1644	N/A	INTRINSIC
internal_repeat_1	1882	2038	6.61e-9	PROSPERO
SCOP:d1gw5a_	2105	2577	9e-5	SMART
internal_repeat_1	2973	3150	6.61e-9	PROSPERO
low complexity region	3561	3580	N/A	INTRINSIC
low complexity region	3928	3936	N/A	INTRINSIC
Pfam:ApoB100_C	4401	4456	5.6e-34	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000037811
AA Change: T10A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000036044
Gene: ENSMUSG00000020609
AA Change: T10A

Domain	Start	End	E-Value	Type
signal peptide	1	27	N/A	INTRINSIC
LPD_N	46	598	6.03e-94	SMART
DUF1943	632	945	7.88e-97	SMART
Pfam:DUF1081	960	1070	6.3e-39	PFAM
low complexity region	1113	1122	N/A	INTRINSIC
Blast:LPD_N	1282	1344	1e-21	BLAST
low complexity region	1665	1677	N/A	INTRINSIC
internal_repeat_1	1915	2071	6.6e-9	PROSPERO
SCOP:d1gw5a_	2138	2610	9e-5	SMART
internal_repeat_1	3006	3183	6.6e-9	PROSPERO
low complexity region	3594	3613	N/A	INTRINSIC
low complexity region	3961	3969	N/A	INTRINSIC
Pfam:ApoB100_C	4434	4490	1.6e-32	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000171271
AA Change: T10A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000127147
Gene: ENSMUSG00000020609
AA Change: T10A

Domain	Start	End	E-Value	Type
signal peptide	1	27	N/A	INTRINSIC
Pfam:Vitellogenin_N	45	324	6.7e-53	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 97.2%
20x: 95.1%

Validation Efficiency

98% (62/63)

MGI Phenotype

FUNCTION: This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100. Unlike the apoB-48 and apoB-100 structural equivalents in human, which are synthesized exclusively in the gut and liver, respectively, the mouse apoB-48 isoform is also found in mouse liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2179 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous null mutants usually die by midgestation and longer survivors exhibit exencephaly. Heterozygotes show reduced plasma cholesterol and apolipoprotein levels. Single isoform B100 and B48 null mutants are viable. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930444P10Rik	T	C	1: 16,151,183 (GRCm39)		probably benign	Het
4930562C15Rik	A	G	16: 4,667,915 (GRCm39)	I435M	possibly damaging	Het
Adcy5	A	G	16: 35,089,440 (GRCm39)	I546V	probably damaging	Het
Brwd1	C	A	16: 95,803,409 (GRCm39)	D2254Y	possibly damaging	Het
Bub1b	C	A	2: 118,460,470 (GRCm39)	D600E	probably damaging	Het
Cnga1	A	G	5: 72,766,843 (GRCm39)	S199P	probably damaging	Het
Cyp3a11	G	T	5: 145,791,945 (GRCm39)	L483I	probably benign	Het
Depp1	T	C	6: 116,628,992 (GRCm39)	S112P	possibly damaging	Het
Dpysl3	C	A	18: 43,466,016 (GRCm39)	G457V	probably damaging	Het
Eif4g3	C	A	4: 137,824,105 (GRCm39)	P36T	possibly damaging	Het
Epb41	C	A	4: 131,706,246 (GRCm39)	G415C	probably damaging	Het
Erap1	G	A	13: 74,808,494 (GRCm39)	V69M	possibly damaging	Het
F2	A	T	2: 91,460,586 (GRCm39)	Y301*	probably null	Het
F2rl2	A	T	13: 95,837,195 (GRCm39)	N80I	possibly damaging	Het
Fam237b	A	T	5: 5,625,565 (GRCm39)	Y87F	possibly damaging	Het
Gm15446	T	G	5: 110,091,036 (GRCm39)	H429Q	probably damaging	Het
Gpat3	G	A	5: 101,031,290 (GRCm39)	G148D	probably damaging	Het
Gpr26	A	T	7: 131,585,976 (GRCm39)	R315S	probably damaging	Het
Hdac9	A	C	12: 34,487,163 (GRCm39)	H100Q	probably damaging	Het
Heatr5a	A	T	12: 51,994,761 (GRCm39)	V457D	probably benign	Het
Igkv10-96	A	G	6: 68,609,223 (GRCm39)	M24T	possibly damaging	Het
Igkv2-95-2	A	G	6: 68,625,095 (GRCm39)		noncoding transcript	Het
Kdm4a	G	A	4: 118,003,802 (GRCm39)	S758F	probably damaging	Het
Lrg1	A	G	17: 56,427,154 (GRCm39)	W273R	probably damaging	Het
Man2a1	A	T	17: 65,017,729 (GRCm39)	Q658H	probably benign	Het
Mapkapk5	T	C	5: 121,671,454 (GRCm39)		probably null	Het
Mcm6	T	C	1: 128,261,321 (GRCm39)	D761G	possibly damaging	Het
Myh8	A	T	11: 67,175,310 (GRCm39)	Y286F	probably damaging	Het
Nbn	C	T	4: 15,963,893 (GRCm39)	T98I	probably damaging	Het
Nrdc	T	C	4: 108,906,305 (GRCm39)	S685P	probably damaging	Het
Nudcd3	A	G	11: 6,143,238 (GRCm39)	V80A	probably benign	Het
Or10al5	A	G	17: 38,063,192 (GRCm39)	H149R	probably benign	Het
Or5p54	A	G	7: 107,554,376 (GRCm39)	H176R	probably damaging	Het
Pdcd6ip	A	T	9: 113,507,401 (GRCm39)	M390K	probably damaging	Het
Pon1	C	T	6: 5,177,295 (GRCm39)	V205I	possibly damaging	Het
Ppp4r2	C	A	6: 100,842,176 (GRCm39)	H212Q	probably benign	Het
Pramel32	T	C	4: 88,548,372 (GRCm39)	D11G	possibly damaging	Het
Prl2c5	G	A	13: 13,360,441 (GRCm39)	C33Y	probably damaging	Het
Rbak	A	G	5: 143,159,917 (GRCm39)	F379L	probably damaging	Het
Rcc2	G	A	4: 140,444,340 (GRCm39)	D344N	probably damaging	Het
Rgs3	T	C	4: 62,620,424 (GRCm39)	L550P	probably damaging	Het
Rnasek	A	T	11: 70,130,486 (GRCm39)	M25K	probably damaging	Het
Scgb1b24	A	T	7: 33,443,520 (GRCm39)	T60S	possibly damaging	Het
Scn5a	A	G	9: 119,365,042 (GRCm39)	F392S	probably damaging	Het
Scpep1	A	T	11: 88,827,871 (GRCm39)	V209E	probably damaging	Het
Slc35g1	A	G	19: 38,391,080 (GRCm39)		probably null	Het
Sorbs1	A	G	19: 40,329,151 (GRCm39)	I554T	probably damaging	Het
Spats1	A	G	17: 45,777,059 (GRCm39)		probably benign	Het
Tdp1	A	G	12: 99,859,919 (GRCm39)	Q202R	probably damaging	Het
Trav6-5	T	G	14: 53,729,045 (GRCm39)	S102A	probably damaging	Het
Tspan2	T	C	3: 102,676,215 (GRCm39)	M208T	probably damaging	Het
Ube4b	A	G	4: 149,471,635 (GRCm39)	S84P	probably damaging	Het
Vmn1r237	C	A	17: 21,534,633 (GRCm39)	Q119K	probably benign	Het
Vmn2r125	C	T	4: 156,703,333 (GRCm39)	T237I	probably benign	Het
Zfp65	A	T	13: 67,856,929 (GRCm39)	S117T	probably benign	Het

Other mutations in Apob

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00402:Apob	APN	12	8,043,065 (GRCm39)	splice site	probably benign
IGL00421:Apob	APN	12	8,060,197 (GRCm39)	missense	probably damaging	0.99
IGL00658:Apob	APN	12	8,059,471 (GRCm39)	missense	probably benign	0.08
IGL00768:Apob	APN	12	8,052,107 (GRCm39)	missense	probably damaging	1.00
IGL00833:Apob	APN	12	8,060,101 (GRCm39)	missense	probably benign	0.14
IGL00926:Apob	APN	12	8,065,421 (GRCm39)	missense	probably benign	0.01
IGL01065:Apob	APN	12	8,053,299 (GRCm39)	missense	probably damaging	0.99
IGL01313:Apob	APN	12	8,050,898 (GRCm39)	missense	probably damaging	1.00
IGL01419:Apob	APN	12	8,052,251 (GRCm39)	missense	probably damaging	0.99
IGL01461:Apob	APN	12	8,051,884 (GRCm39)	missense	probably benign	0.13
IGL02002:Apob	APN	12	8,044,822 (GRCm39)	missense	probably benign	0.03
IGL02031:Apob	APN	12	8,065,222 (GRCm39)	missense	probably benign
IGL02102:Apob	APN	12	8,039,407 (GRCm39)	missense	possibly damaging	0.94
IGL02115:Apob	APN	12	8,042,923 (GRCm39)	missense	probably benign	0.06
IGL02513:Apob	APN	12	8,042,979 (GRCm39)	missense	probably benign	0.01
IGL02967:Apob	APN	12	8,065,366 (GRCm39)	nonsense	probably null
IGL03005:Apob	APN	12	8,043,059 (GRCm39)	splice site	probably benign
IGL03011:Apob	APN	12	8,047,883 (GRCm39)	missense	probably damaging	1.00
IGL03116:Apob	APN	12	8,066,350 (GRCm39)	missense	probably damaging	0.98
IGL03215:Apob	APN	12	8,063,818 (GRCm39)	missense	possibly damaging	0.92
IGL03227:Apob	APN	12	8,066,089 (GRCm39)	missense	probably benign	0.04
Aesthete	UTSW	12	8,060,080 (GRCm39)	nonsense	probably null
Essence	UTSW	12	8,057,769 (GRCm39)	nonsense	probably null
Ethos	UTSW	12	8,040,394 (GRCm39)	missense	probably null	1.00
IGL02835:Apob	UTSW	12	8,065,097 (GRCm39)	missense	possibly damaging	0.86
IGL02837:Apob	UTSW	12	8,055,102 (GRCm39)	missense	probably damaging	1.00
R0071:Apob	UTSW	12	8,052,111 (GRCm39)	missense	probably damaging	0.98
R0071:Apob	UTSW	12	8,052,111 (GRCm39)	missense	probably damaging	0.98
R0116:Apob	UTSW	12	8,039,113 (GRCm39)	unclassified	probably benign
R0180:Apob	UTSW	12	8,058,285 (GRCm39)	nonsense	probably null
R0288:Apob	UTSW	12	8,040,779 (GRCm39)	nonsense	probably null
R0295:Apob	UTSW	12	8,052,181 (GRCm39)	nonsense	probably null
R0305:Apob	UTSW	12	8,062,210 (GRCm39)	missense	probably damaging	1.00
R0312:Apob	UTSW	12	8,059,034 (GRCm39)	missense	probably benign
R0324:Apob	UTSW	12	8,060,521 (GRCm39)	missense	probably benign	0.41
R0326:Apob	UTSW	12	8,040,307 (GRCm39)	missense	probably damaging	1.00
R0363:Apob	UTSW	12	8,060,136 (GRCm39)	missense	probably damaging	1.00
R0390:Apob	UTSW	12	8,038,678 (GRCm39)	missense	probably damaging	0.99
R0462:Apob	UTSW	12	8,050,896 (GRCm39)	missense	probably damaging	1.00
R0471:Apob	UTSW	12	8,040,406 (GRCm39)	missense	probably damaging	1.00
R0532:Apob	UTSW	12	8,066,188 (GRCm39)	missense	possibly damaging	0.48
R0548:Apob	UTSW	12	8,056,282 (GRCm39)	missense	probably damaging	1.00
R0560:Apob	UTSW	12	8,055,101 (GRCm39)	missense	probably damaging	1.00
R0595:Apob	UTSW	12	8,058,369 (GRCm39)	missense	probably benign	0.01
R0600:Apob	UTSW	12	8,056,440 (GRCm39)	missense	probably damaging	1.00
R0626:Apob	UTSW	12	8,066,193 (GRCm39)	missense	probably benign	0.45
R0685:Apob	UTSW	12	8,060,742 (GRCm39)	missense	probably benign
R0765:Apob	UTSW	12	8,066,518 (GRCm39)	missense	probably benign
R0790:Apob	UTSW	12	8,060,245 (GRCm39)	missense	probably damaging	1.00
R0918:Apob	UTSW	12	8,033,941 (GRCm39)	missense	probably benign	0.10
R0962:Apob	UTSW	12	8,039,191 (GRCm39)	missense	probably damaging	0.98
R1055:Apob	UTSW	12	8,044,963 (GRCm39)	missense	probably damaging	1.00
R1077:Apob	UTSW	12	8,056,017 (GRCm39)	missense	probably benign
R1143:Apob	UTSW	12	8,062,354 (GRCm39)	missense	probably benign	0.26
R1163:Apob	UTSW	12	8,061,654 (GRCm39)	missense	probably damaging	1.00
R1266:Apob	UTSW	12	8,056,093 (GRCm39)	missense	probably benign	0.37
R1434:Apob	UTSW	12	8,059,715 (GRCm39)	missense	probably damaging	1.00
R1442:Apob	UTSW	12	8,036,165 (GRCm39)	missense	probably benign	0.31
R1445:Apob	UTSW	12	8,066,084 (GRCm39)	missense	possibly damaging	0.48
R1459:Apob	UTSW	12	8,061,937 (GRCm39)	missense	possibly damaging	0.92
R1459:Apob	UTSW	12	8,056,047 (GRCm39)	missense	probably benign
R1465:Apob	UTSW	12	8,061,421 (GRCm39)	missense	possibly damaging	0.91
R1465:Apob	UTSW	12	8,061,421 (GRCm39)	missense	possibly damaging	0.91
R1508:Apob	UTSW	12	8,061,481 (GRCm39)	missense	possibly damaging	0.92
R1518:Apob	UTSW	12	8,039,207 (GRCm39)	missense	probably benign	0.01
R1531:Apob	UTSW	12	8,047,880 (GRCm39)	missense	possibly damaging	0.65
R1547:Apob	UTSW	12	8,053,368 (GRCm39)	missense	probably benign	0.08
R1574:Apob	UTSW	12	8,040,839 (GRCm39)	missense	possibly damaging	0.51
R1574:Apob	UTSW	12	8,040,839 (GRCm39)	missense	possibly damaging	0.51
R1682:Apob	UTSW	12	8,062,365 (GRCm39)	missense	probably benign	0.00
R1709:Apob	UTSW	12	8,059,306 (GRCm39)	missense	probably damaging	0.98
R1718:Apob	UTSW	12	8,066,087 (GRCm39)	missense	probably benign	0.02
R1752:Apob	UTSW	12	8,038,766 (GRCm39)	missense	probably benign	0.01
R1781:Apob	UTSW	12	8,059,603 (GRCm39)	missense	possibly damaging	0.96
R1818:Apob	UTSW	12	8,063,064 (GRCm39)	missense	possibly damaging	0.93
R1818:Apob	UTSW	12	8,056,834 (GRCm39)	missense	probably damaging	0.98
R1842:Apob	UTSW	12	8,061,559 (GRCm39)	missense	probably damaging	1.00
R1843:Apob	UTSW	12	8,057,602 (GRCm39)	missense	possibly damaging	0.65
R1853:Apob	UTSW	12	8,060,928 (GRCm39)	nonsense	probably null
R1990:Apob	UTSW	12	8,051,039 (GRCm39)	missense	probably damaging	1.00
R2016:Apob	UTSW	12	8,057,751 (GRCm39)	missense	possibly damaging	0.48
R2017:Apob	UTSW	12	8,057,751 (GRCm39)	missense	possibly damaging	0.48
R2023:Apob	UTSW	12	8,061,090 (GRCm39)	missense	probably benign	0.01
R2037:Apob	UTSW	12	8,057,488 (GRCm39)	missense	probably benign	0.37
R2054:Apob	UTSW	12	8,063,134 (GRCm39)	missense	probably damaging	1.00
R2057:Apob	UTSW	12	8,052,164 (GRCm39)	nonsense	probably null
R2085:Apob	UTSW	12	8,062,240 (GRCm39)	missense	probably damaging	1.00
R2159:Apob	UTSW	12	8,060,081 (GRCm39)	missense	probably benign	0.12
R2209:Apob	UTSW	12	8,057,752 (GRCm39)	missense	probably benign	0.28
R2249:Apob	UTSW	12	8,057,499 (GRCm39)	missense	probably damaging	1.00
R2254:Apob	UTSW	12	8,061,256 (GRCm39)	missense	possibly damaging	0.92
R2265:Apob	UTSW	12	8,065,475 (GRCm39)	missense	possibly damaging	0.74
R2266:Apob	UTSW	12	8,065,475 (GRCm39)	missense	possibly damaging	0.74
R2267:Apob	UTSW	12	8,065,475 (GRCm39)	missense	possibly damaging	0.74
R2268:Apob	UTSW	12	8,065,475 (GRCm39)	missense	possibly damaging	0.74
R2296:Apob	UTSW	12	8,044,879 (GRCm39)	missense	probably damaging	0.97
R2897:Apob	UTSW	12	8,060,356 (GRCm39)	missense	probably damaging	1.00
R3431:Apob	UTSW	12	8,060,778 (GRCm39)	missense	probably damaging	1.00
R3723:Apob	UTSW	12	8,056,327 (GRCm39)	missense	probably damaging	1.00
R3723:Apob	UTSW	12	8,061,763 (GRCm39)	missense	possibly damaging	0.46
R3899:Apob	UTSW	12	8,065,849 (GRCm39)	missense	possibly damaging	0.87
R4020:Apob	UTSW	12	8,044,914 (GRCm39)	nonsense	probably null
R4050:Apob	UTSW	12	8,065,390 (GRCm39)	missense	probably benign	0.02
R4351:Apob	UTSW	12	8,043,054 (GRCm39)	missense	probably benign	0.03
R4365:Apob	UTSW	12	8,066,083 (GRCm39)	missense	possibly damaging	0.95
R4366:Apob	UTSW	12	8,066,083 (GRCm39)	missense	possibly damaging	0.95
R4456:Apob	UTSW	12	8,065,445 (GRCm39)	missense	probably damaging	1.00
R4458:Apob	UTSW	12	8,065,445 (GRCm39)	missense	probably damaging	1.00
R4600:Apob	UTSW	12	8,058,568 (GRCm39)	missense	probably damaging	1.00
R4611:Apob	UTSW	12	8,061,331 (GRCm39)	missense	probably damaging	1.00
R4646:Apob	UTSW	12	8,062,759 (GRCm39)	missense	probably benign	0.21
R4678:Apob	UTSW	12	8,045,585 (GRCm39)	missense	probably damaging	1.00
R4685:Apob	UTSW	12	8,056,456 (GRCm39)	missense	probably benign	0.00
R4707:Apob	UTSW	12	8,056,205 (GRCm39)	missense	probably damaging	0.96
R4726:Apob	UTSW	12	8,040,267 (GRCm39)	missense	probably damaging	0.98
R4792:Apob	UTSW	12	8,058,051 (GRCm39)	missense	probably benign	0.26
R4822:Apob	UTSW	12	8,065,741 (GRCm39)	missense	probably benign	0.04
R4834:Apob	UTSW	12	8,064,101 (GRCm39)	missense	possibly damaging	0.49
R4835:Apob	UTSW	12	8,065,391 (GRCm39)	missense	possibly damaging	0.56
R4887:Apob	UTSW	12	8,063,099 (GRCm39)	missense	probably damaging	1.00
R4910:Apob	UTSW	12	8,057,848 (GRCm39)	missense	probably damaging	1.00
R5072:Apob	UTSW	12	8,058,714 (GRCm39)	missense	probably benign	0.00
R5073:Apob	UTSW	12	8,055,219 (GRCm39)	critical splice donor site	probably null
R5074:Apob	UTSW	12	8,055,219 (GRCm39)	critical splice donor site	probably null
R5101:Apob	UTSW	12	8,061,934 (GRCm39)	missense	probably benign	0.09
R5123:Apob	UTSW	12	8,057,630 (GRCm39)	splice site	probably null
R5133:Apob	UTSW	12	8,058,898 (GRCm39)	missense	probably damaging	0.99
R5135:Apob	UTSW	12	8,060,086 (GRCm39)	missense	probably damaging	1.00
R5137:Apob	UTSW	12	8,061,384 (GRCm39)	missense	possibly damaging	0.63
R5160:Apob	UTSW	12	8,062,126 (GRCm39)	missense	possibly damaging	0.90
R5173:Apob	UTSW	12	8,058,238 (GRCm39)	missense	probably benign	0.00
R5202:Apob	UTSW	12	8,063,737 (GRCm39)	missense	probably damaging	0.98
R5292:Apob	UTSW	12	8,055,912 (GRCm39)	missense	probably benign	0.01
R5378:Apob	UTSW	12	8,061,865 (GRCm39)	missense	probably damaging	0.99
R5494:Apob	UTSW	12	8,061,762 (GRCm39)	missense	probably damaging	0.99
R5517:Apob	UTSW	12	8,040,906 (GRCm39)	missense	probably damaging	1.00
R5576:Apob	UTSW	12	8,048,662 (GRCm39)	missense	probably damaging	1.00
R5582:Apob	UTSW	12	8,060,788 (GRCm39)	missense	probably damaging	1.00
R5629:Apob	UTSW	12	8,057,847 (GRCm39)	missense	probably damaging	1.00
R5678:Apob	UTSW	12	8,041,494 (GRCm39)	missense	possibly damaging	0.92
R5732:Apob	UTSW	12	8,060,353 (GRCm39)	missense	probably benign	0.15
R5734:Apob	UTSW	12	8,038,781 (GRCm39)	missense	probably damaging	1.00
R5742:Apob	UTSW	12	8,057,191 (GRCm39)	missense	probably damaging	1.00
R5751:Apob	UTSW	12	8,062,619 (GRCm39)	nonsense	probably null
R5776:Apob	UTSW	12	8,056,149 (GRCm39)	missense	possibly damaging	0.57
R5778:Apob	UTSW	12	8,065,074 (GRCm39)	missense	probably benign	0.45
R5783:Apob	UTSW	12	8,051,022 (GRCm39)	missense	probably damaging	1.00
R5786:Apob	UTSW	12	8,065,304 (GRCm39)	missense	possibly damaging	0.48
R5837:Apob	UTSW	12	8,053,277 (GRCm39)	missense	probably benign	0.04
R5857:Apob	UTSW	12	8,065,397 (GRCm39)	missense	probably benign	0.00
R6029:Apob	UTSW	12	8,066,243 (GRCm39)	missense	probably damaging	0.99
R6032:Apob	UTSW	12	8,045,513 (GRCm39)	missense	probably benign	0.02
R6032:Apob	UTSW	12	8,045,513 (GRCm39)	missense	probably benign	0.02
R6086:Apob	UTSW	12	8,065,164 (GRCm39)	missense	probably benign
R6110:Apob	UTSW	12	8,061,883 (GRCm39)	missense	probably damaging	1.00
R6131:Apob	UTSW	12	8,065,874 (GRCm39)	missense	probably benign	0.17
R6157:Apob	UTSW	12	8,056,077 (GRCm39)	missense	probably benign
R6179:Apob	UTSW	12	8,055,060 (GRCm39)	nonsense	probably null
R6247:Apob	UTSW	12	8,051,801 (GRCm39)	missense	probably damaging	1.00
R6279:Apob	UTSW	12	8,057,769 (GRCm39)	nonsense	probably null
R6300:Apob	UTSW	12	8,057,769 (GRCm39)	nonsense	probably null
R6320:Apob	UTSW	12	8,039,194 (GRCm39)	missense	probably benign	0.27
R6339:Apob	UTSW	12	8,066,188 (GRCm39)	missense	probably damaging	0.99
R6353:Apob	UTSW	12	8,059,421 (GRCm39)	missense	probably damaging	1.00
R6395:Apob	UTSW	12	8,058,507 (GRCm39)	missense	probably benign	0.45
R6441:Apob	UTSW	12	8,037,796 (GRCm39)	missense	probably damaging	1.00
R6492:Apob	UTSW	12	8,058,261 (GRCm39)	missense	probably damaging	0.99
R6495:Apob	UTSW	12	8,040,394 (GRCm39)	missense	probably null	1.00
R6502:Apob	UTSW	12	8,051,814 (GRCm39)	missense	probably damaging	0.99
R6520:Apob	UTSW	12	8,033,124 (GRCm39)	missense	probably damaging	1.00
R6644:Apob	UTSW	12	8,059,077 (GRCm39)	missense	probably damaging	0.97
R6704:Apob	UTSW	12	8,060,379 (GRCm39)	missense	probably damaging	0.98
R6750:Apob	UTSW	12	8,047,853 (GRCm39)	missense	probably damaging	1.00
R6759:Apob	UTSW	12	8,061,049 (GRCm39)	missense	probably benign	0.06
R6812:Apob	UTSW	12	8,033,062 (GRCm39)	missense	probably damaging	0.98
R6865:Apob	UTSW	12	8,058,847 (GRCm39)	missense	probably benign	0.05
R6873:Apob	UTSW	12	8,065,995 (GRCm39)	missense	probably benign	0.00
R7013:Apob	UTSW	12	8,060,080 (GRCm39)	nonsense	probably null
R7067:Apob	UTSW	12	8,059,423 (GRCm39)	missense	probably damaging	1.00
R7084:Apob	UTSW	12	8,059,591 (GRCm39)	missense	probably benign
R7113:Apob	UTSW	12	8,045,539 (GRCm39)	missense	probably damaging	1.00
R7175:Apob	UTSW	12	8,057,034 (GRCm39)	missense	probably benign	0.33
R7196:Apob	UTSW	12	8,033,893 (GRCm39)	missense	possibly damaging	0.90
R7199:Apob	UTSW	12	8,055,072 (GRCm39)	missense	probably damaging	1.00
R7205:Apob	UTSW	12	8,055,087 (GRCm39)	missense	probably damaging	0.98
R7251:Apob	UTSW	12	8,057,037 (GRCm39)	missense	probably damaging	0.98
R7474:Apob	UTSW	12	8,059,185 (GRCm39)	missense	probably benign	0.29
R7484:Apob	UTSW	12	8,056,884 (GRCm39)	nonsense	probably null
R7538:Apob	UTSW	12	8,052,219 (GRCm39)	missense	probably damaging	0.98
R7636:Apob	UTSW	12	8,059,516 (GRCm39)	missense	possibly damaging	0.86
R7646:Apob	UTSW	12	8,059,189 (GRCm39)	missense	probably damaging	0.99
R7787:Apob	UTSW	12	8,040,780 (GRCm39)	missense	probably damaging	0.97
R7793:Apob	UTSW	12	8,058,124 (GRCm39)	missense	probably damaging	0.99
R7836:Apob	UTSW	12	8,051,885 (GRCm39)	missense	possibly damaging	0.72
R7895:Apob	UTSW	12	8,061,933 (GRCm39)	missense	probably benign	0.00
R8005:Apob	UTSW	12	8,059,744 (GRCm39)	missense	probably benign	0.01
R8013:Apob	UTSW	12	8,060,798 (GRCm39)	missense	possibly damaging	0.94
R8014:Apob	UTSW	12	8,060,798 (GRCm39)	missense	possibly damaging	0.94
R8111:Apob	UTSW	12	8,058,801 (GRCm39)	missense	probably benign	0.16
R8117:Apob	UTSW	12	8,056,435 (GRCm39)	missense	probably damaging	0.99
R8226:Apob	UTSW	12	8,059,056 (GRCm39)	missense	probably benign	0.00
R8244:Apob	UTSW	12	8,060,548 (GRCm39)	missense	probably damaging	0.96
R8280:Apob	UTSW	12	8,060,851 (GRCm39)	missense	possibly damaging	0.46
R8310:Apob	UTSW	12	8,059,033 (GRCm39)	missense	probably benign	0.00
R8327:Apob	UTSW	12	8,051,015 (GRCm39)	missense	possibly damaging	0.72
R8329:Apob	UTSW	12	8,061,135 (GRCm39)	missense	probably damaging	0.98
R8331:Apob	UTSW	12	8,051,882 (GRCm39)	missense	probably benign	0.28
R8351:Apob	UTSW	12	8,056,356 (GRCm39)	missense	probably benign	0.29
R8412:Apob	UTSW	12	8,058,069 (GRCm39)	missense	probably benign	0.33
R8425:Apob	UTSW	12	8,038,842 (GRCm39)	missense	possibly damaging	0.70
R8481:Apob	UTSW	12	8,044,807 (GRCm39)	splice site	probably null
R8493:Apob	UTSW	12	8,059,009 (GRCm39)	missense	possibly damaging	0.87
R8529:Apob	UTSW	12	8,057,353 (GRCm39)	missense	probably damaging	1.00
R8554:Apob	UTSW	12	8,037,830 (GRCm39)	missense	probably damaging	0.98
R8692:Apob	UTSW	12	8,058,270 (GRCm39)	missense	probably damaging	0.98
R8695:Apob	UTSW	12	8,057,830 (GRCm39)	missense	probably damaging	1.00
R8977:Apob	UTSW	12	8,065,990 (GRCm39)	missense	probably damaging	0.99
R9016:Apob	UTSW	12	8,035,408 (GRCm39)	splice site	silent
R9020:Apob	UTSW	12	8,063,999 (GRCm39)	missense	probably damaging	1.00
R9037:Apob	UTSW	12	8,066,501 (GRCm39)	missense	probably benign	0.15
R9053:Apob	UTSW	12	8,058,954 (GRCm39)	missense	possibly damaging	0.72
R9062:Apob	UTSW	12	8,058,046 (GRCm39)	missense	possibly damaging	0.91
R9142:Apob	UTSW	12	8,062,705 (GRCm39)	missense	possibly damaging	0.95
R9180:Apob	UTSW	12	8,047,925 (GRCm39)	missense	probably damaging	1.00
R9205:Apob	UTSW	12	8,030,635 (GRCm39)	missense	probably damaging	0.99
R9248:Apob	UTSW	12	8,065,231 (GRCm39)	nonsense	probably null
R9277:Apob	UTSW	12	8,061,183 (GRCm39)	missense	probably benign	0.01
R9305:Apob	UTSW	12	8,058,053 (GRCm39)	missense	probably benign	0.04
R9358:Apob	UTSW	12	8,060,833 (GRCm39)	missense	probably benign	0.14
R9375:Apob	UTSW	12	8,029,261 (GRCm39)	missense	possibly damaging	0.91
R9385:Apob	UTSW	12	8,056,399 (GRCm39)	missense	possibly damaging	0.91
R9386:Apob	UTSW	12	8,056,629 (GRCm39)	missense	probably damaging	0.99
R9392:Apob	UTSW	12	8,057,098 (GRCm39)	missense	probably benign	0.45
R9470:Apob	UTSW	12	8,039,219 (GRCm39)	missense	possibly damaging	0.94
R9523:Apob	UTSW	12	8,052,069 (GRCm39)	missense	probably damaging	1.00
R9545:Apob	UTSW	12	8,033,890 (GRCm39)	missense	possibly damaging	0.81
R9629:Apob	UTSW	12	8,059,054 (GRCm39)	missense	probably damaging	1.00
R9702:Apob	UTSW	12	8,057,559 (GRCm39)	missense	probably damaging	0.96
R9703:Apob	UTSW	12	8,030,507 (GRCm39)	missense	probably damaging	0.99
R9719:Apob	UTSW	12	8,065,464 (GRCm39)	missense	probably benign	0.15
R9726:Apob	UTSW	12	8,056,926 (GRCm39)	missense	probably damaging	0.99
R9729:Apob	UTSW	12	8,066,125 (GRCm39)	missense	probably damaging	0.99
X0027:Apob	UTSW	12	8,057,975 (GRCm39)	missense	probably benign
Z1088:Apob	UTSW	12	8,062,936 (GRCm39)	missense	possibly damaging	0.95
Z1088:Apob	UTSW	12	8,055,945 (GRCm39)	nonsense	probably null
Z1088:Apob	UTSW	12	8,055,074 (GRCm39)	missense	possibly damaging	0.91
Z1176:Apob	UTSW	12	8,054,978 (GRCm39)	missense	probably benign	0.00
Z1176:Apob	UTSW	12	8,048,011 (GRCm39)	missense	probably damaging	1.00
Z1177:Apob	UTSW	12	8,065,249 (GRCm39)	frame shift	probably null
Z1177:Apob	UTSW	12	8,038,765 (GRCm39)	missense	probably benign	0.43

Predicted Primers

PCR Primer
(F):5'- CCTAGAAATGCGCTTGTATGG -3'
(R):5'- TACCTAATAGCCAGCCTGCC -3'

Sequencing Primer
(F):5'- TTAAGCCAGCTCCTCCTATAAATGG -3'
(R):5'- CACCAGTTTCTACCCCAGGATG -3'

Posted On

2016-07-22