Mutagenetix > Incidental Mutation

Incidental Mutation 'R5277:Vmn2r75'

404000

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r75

Ensembl Gene

ENSMUSG00000090436

Gene Name

vomeronasal 2, receptor 75

Synonyms

EG546981

MMRRC Submission

042864-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.090) question?

Stock #

R5277 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

85797250-85820932 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to C at 85815500 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Arginine at position 121 (S121R)

Ref Sequence

ENSEMBL: ENSMUSP00000126973 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000167830]

AlphaFold

G5E8Z7

Predicted Effect

probably benign
Transcript: ENSMUST00000167830
AA Change: S121R

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000126973
Gene: ENSMUSG00000090436
AA Change: S121R

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
Pfam:ANF_receptor	80	466	2.8e-31	PFAM
Pfam:NCD3G	510	562	4.6e-20	PFAM
Pfam:7tm_3	593	829	7.7e-51	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.5%
20x: 95.9%

Validation Efficiency

98% (52/53)

Allele List at MGI

Other mutations in this stock

Total: 45 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abi1	T	C	2: 22,884,660 (GRCm39)	T54A	probably damaging	Het
Ablim1	T	C	19: 57,143,693 (GRCm39)	R89G	probably damaging	Het
Bco1	T	A	8: 117,844,128 (GRCm39)		probably null	Het
Bhmt	A	T	13: 93,761,393 (GRCm39)	M185K	possibly damaging	Het
Camk2d	T	C	3: 126,478,390 (GRCm39)		probably benign	Het
Cmtm1	CGGCACGTACTGAAGGTCGCTGACTGGATGGTGTGGCACGTACTGAAGGTCGCTGACTGGATGGTGTGGCACGTACTGAAGGTCGCTGACTGGATGGT	CGGCACGTACTGAAGGTCGCTGACTGGATGGTGTGGCACGTACTGAAGGTCGCTGACTGGATGGT	8: 105,036,102 (GRCm39)		probably benign	Het
Dcaf6	A	G	1: 165,251,915 (GRCm39)	S70P	probably benign	Het
Dclre1a	C	T	19: 56,533,164 (GRCm39)	V477I	possibly damaging	Het
Dnah10	C	T	5: 124,905,201 (GRCm39)	P4021L	probably damaging	Het
Dusp10	A	G	1: 183,769,204 (GRCm39)	N57D	possibly damaging	Het
Fam13b	G	A	18: 34,595,243 (GRCm39)	R374C	probably benign	Het
Fyb2	G	A	4: 104,872,876 (GRCm39)	D686N	probably damaging	Het
Glra3	G	A	8: 56,444,242 (GRCm39)	M67I	possibly damaging	Het
Gm14325	G	A	2: 177,474,777 (GRCm39)	H102Y	possibly damaging	Het
Gm6180	A	G	8: 42,700,177 (GRCm39)		noncoding transcript	Het
Grin2c	A	G	11: 115,144,639 (GRCm39)	V629A	probably damaging	Het
Kif4-ps	A	T	12: 101,112,186 (GRCm39)		noncoding transcript	Het
Mc3r	T	C	2: 172,091,707 (GRCm39)	F310L	probably damaging	Het
Myh4	A	G	11: 67,143,180 (GRCm39)	D1036G	probably damaging	Het
Myh6	A	G	14: 55,194,019 (GRCm39)	I790T	probably benign	Het
Myo15a	C	A	11: 60,367,940 (GRCm39)	Y233*	probably null	Het
Nckap5	A	T	1: 125,954,277 (GRCm39)	C758*	probably null	Het
Neurog3	T	A	10: 61,969,632 (GRCm39)	Y36N	probably damaging	Het
Nlrp4e	T	C	7: 23,020,863 (GRCm39)	L450P	probably benign	Het
Or52d13	A	T	7: 103,110,148 (GRCm39)	L84H	probably damaging	Het
Otog	A	G	7: 45,896,045 (GRCm39)	E170G	possibly damaging	Het
Ppp2r2b	G	T	18: 42,874,207 (GRCm39)	T41K	probably damaging	Het
Prmt5	A	T	14: 54,747,399 (GRCm39)	D459E	probably benign	Het
Ric8b	G	A	10: 84,783,516 (GRCm39)	V125M	probably damaging	Het
Rptor	A	G	11: 119,713,782 (GRCm39)	S4G	probably damaging	Het
Rslcan18	T	G	13: 67,246,498 (GRCm39)	E371D	probably benign	Het
Scaf11	A	T	15: 96,317,107 (GRCm39)	F819Y	probably damaging	Het
Sema6a	A	T	18: 47,409,611 (GRCm39)		probably benign	Het
Snx29	C	T	16: 11,217,688 (GRCm39)	T163I	possibly damaging	Het
Sphkap	T	C	1: 83,253,885 (GRCm39)	N1288S	probably benign	Het
Tmbim7	G	A	5: 3,723,192 (GRCm39)		probably null	Het
Tmem63c	T	A	12: 87,104,531 (GRCm39)		probably null	Het
Tmem69	A	G	4: 116,410,458 (GRCm39)	F171L	probably benign	Het
Urod	A	T	4: 116,847,482 (GRCm39)		probably benign	Het
Vmn1r37	A	T	6: 66,708,460 (GRCm39)	I29L	probably benign	Het
Vmn2r102	A	G	17: 19,914,393 (GRCm39)	T653A	possibly damaging	Het
Vwde	T	C	6: 13,186,995 (GRCm39)	T831A	probably benign	Het
Wdr70	C	T	15: 8,006,465 (GRCm39)	W362*	probably null	Het
Zfat	A	G	15: 68,037,758 (GRCm39)	C906R	probably damaging	Het
Zfp7	T	C	15: 76,765,403 (GRCm39)	V32A	probably damaging	Het

Other mutations in Vmn2r75

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01133:Vmn2r75	APN	7	85,797,240 (GRCm39)	unclassified	probably benign
IGL01287:Vmn2r75	APN	7	85,797,801 (GRCm39)	missense	probably damaging	0.97
IGL01318:Vmn2r75	APN	7	85,814,774 (GRCm39)	missense	probably benign	0.06
IGL01331:Vmn2r75	APN	7	85,820,870 (GRCm39)	nonsense	probably null
IGL01406:Vmn2r75	APN	7	85,812,500 (GRCm39)	splice site	probably benign
IGL01615:Vmn2r75	APN	7	85,797,681 (GRCm39)	missense	probably benign	0.03
IGL01657:Vmn2r75	APN	7	85,813,455 (GRCm39)	missense	probably damaging	1.00
IGL02237:Vmn2r75	APN	7	85,814,786 (GRCm39)	missense	possibly damaging	0.88
IGL02275:Vmn2r75	APN	7	85,814,348 (GRCm39)	missense	probably benign	0.04
IGL02307:Vmn2r75	APN	7	85,814,974 (GRCm39)	missense	probably benign	0.00
IGL03136:Vmn2r75	APN	7	85,797,911 (GRCm39)	missense	possibly damaging	0.89
IGL03160:Vmn2r75	APN	7	85,797,644 (GRCm39)	missense	probably damaging	1.00
IGL03244:Vmn2r75	APN	7	85,820,933 (GRCm39)	unclassified	probably benign
PIT4449001:Vmn2r75	UTSW	7	85,814,791 (GRCm39)	missense	probably damaging	1.00
R0049:Vmn2r75	UTSW	7	85,797,309 (GRCm39)	nonsense	probably null
R0049:Vmn2r75	UTSW	7	85,797,309 (GRCm39)	nonsense	probably null
R0083:Vmn2r75	UTSW	7	85,814,866 (GRCm39)	missense	probably benign	0.00
R0108:Vmn2r75	UTSW	7	85,814,866 (GRCm39)	missense	probably benign	0.00
R0276:Vmn2r75	UTSW	7	85,797,515 (GRCm39)	missense	probably benign	0.01
R0320:Vmn2r75	UTSW	7	85,814,288 (GRCm39)	missense	probably benign	0.36
R0471:Vmn2r75	UTSW	7	85,814,721 (GRCm39)	missense	probably benign	0.01
R0562:Vmn2r75	UTSW	7	85,797,449 (GRCm39)	nonsense	probably null
R0631:Vmn2r75	UTSW	7	85,812,478 (GRCm39)	missense	probably null	1.00
R0661:Vmn2r75	UTSW	7	85,814,866 (GRCm39)	missense	probably benign	0.00
R0811:Vmn2r75	UTSW	7	85,814,575 (GRCm39)	missense	probably benign	0.38
R0812:Vmn2r75	UTSW	7	85,814,575 (GRCm39)	missense	probably benign	0.38
R0891:Vmn2r75	UTSW	7	85,813,476 (GRCm39)	missense	possibly damaging	0.81
R1340:Vmn2r75	UTSW	7	85,797,798 (GRCm39)	missense	probably damaging	0.98
R1501:Vmn2r75	UTSW	7	85,814,850 (GRCm39)	missense	possibly damaging	0.85
R1760:Vmn2r75	UTSW	7	85,798,019 (GRCm39)	missense	probably damaging	1.00
R1970:Vmn2r75	UTSW	7	85,797,470 (GRCm39)	missense	probably damaging	1.00
R2060:Vmn2r75	UTSW	7	85,814,372 (GRCm39)	missense	probably benign	0.00
R2292:Vmn2r75	UTSW	7	85,798,144 (GRCm39)	missense	probably damaging	1.00
R3688:Vmn2r75	UTSW	7	85,797,629 (GRCm39)	missense	probably damaging	0.99
R3892:Vmn2r75	UTSW	7	85,813,494 (GRCm39)	missense	probably null	1.00
R4532:Vmn2r75	UTSW	7	85,797,349 (GRCm39)	nonsense	probably null
R4583:Vmn2r75	UTSW	7	85,813,290 (GRCm39)	missense	possibly damaging	0.81
R4592:Vmn2r75	UTSW	7	85,815,494 (GRCm39)	missense	probably benign	0.00
R4792:Vmn2r75	UTSW	7	85,812,378 (GRCm39)	missense	possibly damaging	0.46
R4859:Vmn2r75	UTSW	7	85,797,611 (GRCm39)	missense	probably benign	0.35
R4896:Vmn2r75	UTSW	7	85,820,787 (GRCm39)	missense	probably benign	0.01
R4943:Vmn2r75	UTSW	7	85,814,705 (GRCm39)	missense	probably damaging	1.00
R4992:Vmn2r75	UTSW	7	85,815,375 (GRCm39)	critical splice donor site	probably null
R5048:Vmn2r75	UTSW	7	85,814,735 (GRCm39)	missense	possibly damaging	0.66
R5063:Vmn2r75	UTSW	7	85,813,372 (GRCm39)	missense	probably benign
R5156:Vmn2r75	UTSW	7	85,813,436 (GRCm39)	missense	possibly damaging	0.51
R5243:Vmn2r75	UTSW	7	85,813,447 (GRCm39)	missense	probably damaging	1.00
R5574:Vmn2r75	UTSW	7	85,815,510 (GRCm39)	missense	probably benign	0.22
R5622:Vmn2r75	UTSW	7	85,797,702 (GRCm39)	missense	probably benign	0.15
R5680:Vmn2r75	UTSW	7	85,820,779 (GRCm39)	missense	probably benign	0.10
R5884:Vmn2r75	UTSW	7	85,814,578 (GRCm39)	missense	probably benign
R6021:Vmn2r75	UTSW	7	85,820,820 (GRCm39)	missense	probably benign	0.01
R6217:Vmn2r75	UTSW	7	85,815,375 (GRCm39)	critical splice donor site	probably benign
R6242:Vmn2r75	UTSW	7	85,814,592 (GRCm39)	missense	probably damaging	1.00
R6299:Vmn2r75	UTSW	7	85,814,482 (GRCm39)	missense	probably benign	0.12
R6441:Vmn2r75	UTSW	7	85,820,784 (GRCm39)	missense	probably damaging	0.99
R6495:Vmn2r75	UTSW	7	85,813,287 (GRCm39)	missense	probably benign	0.00
R6553:Vmn2r75	UTSW	7	85,813,453 (GRCm39)	missense	probably benign	0.28
R6670:Vmn2r75	UTSW	7	85,797,644 (GRCm39)	missense	probably damaging	1.00
R7078:Vmn2r75	UTSW	7	85,815,568 (GRCm39)	missense	probably damaging	1.00
R7164:Vmn2r75	UTSW	7	85,814,592 (GRCm39)	missense	probably damaging	1.00
R8411:Vmn2r75	UTSW	7	85,797,722 (GRCm39)	missense	probably damaging	1.00
R8507:Vmn2r75	UTSW	7	85,797,685 (GRCm39)	nonsense	probably null
R8559:Vmn2r75	UTSW	7	85,815,480 (GRCm39)	missense	possibly damaging	0.65
R8677:Vmn2r75	UTSW	7	85,814,410 (GRCm39)	missense	possibly damaging	0.86
R8708:Vmn2r75	UTSW	7	85,812,476 (GRCm39)	missense	probably damaging	0.99
R8778:Vmn2r75	UTSW	7	85,813,497 (GRCm39)	missense	probably benign	0.40
R8968:Vmn2r75	UTSW	7	85,820,765 (GRCm39)	nonsense	probably null
R9145:Vmn2r75	UTSW	7	85,813,447 (GRCm39)	missense	probably damaging	1.00
R9316:Vmn2r75	UTSW	7	85,797,313 (GRCm39)	missense	possibly damaging	0.63
R9363:Vmn2r75	UTSW	7	85,815,423 (GRCm39)	missense	probably benign	0.03

Predicted Primers

PCR Primer
(F):5'- TCCCATGCCCACTTATGATAAAG -3'
(R):5'- TCTGTTTTCAGGGTGACACC -3'

Sequencing Primer
(F):5'- ATTACCTTCATACTGTGGTGAGAAAG -3'
(R):5'- CCCAAGACAAACCATTTAATTTTCTC -3'

Posted On

2016-07-22