Mutagenetix > Incidental Mutations

Incidental Mutation 'R5280:Vmn2r73'

404163

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r73

Ensembl Gene

ENSMUSG00000070458

Gene Name

vomeronasal 2, receptor 73

Synonyms

EG620928

MMRRC Submission

042840-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.207) question?

Stock #

R5280 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

85857547-85875938 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 85857947 bp

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 719 (D719G)

Ref Sequence

ENSEMBL: ENSMUSP00000076687 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000077478]

Predicted Effect

probably damaging
Transcript: ENSMUST00000077478
AA Change: D719G

PolyPhen 2 Score 0.979 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000076687
Gene: ENSMUSG00000070458
AA Change: D719G

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	465	1.4e-30	PFAM
Pfam:NCD3G	508	560	5.9e-21	PFAM
Pfam:7tm_3	590	827	4.4e-51	PFAM

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.3%
20x: 95.5%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 33 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrf5	C	T	17: 43,426,334	T218I	probably damaging	Het
Adgrv1	C	A	13: 81,397,465	S5567I	possibly damaging	Het
Alpk1	T	C	3: 127,681,164	T397A	probably benign	Het
Atp9a	T	A	2: 168,639,988	Y880F	possibly damaging	Het
Celsr1	T	C	15: 85,930,546	T1905A	probably benign	Het
Chd8	G	A	14: 52,205,125	A656V	possibly damaging	Het
Dffa	A	G	4: 149,117,934	N265S	probably benign	Het
Frrs1	A	G	3: 116,880,896	D109G	probably benign	Het
Gm20821	A	T	Y: 9,783,963	K195M	probably damaging	Het
Ifi207	A	T	1: 173,730,304	N289K	unknown	Het
Kank1	T	C	19: 25,411,305	F781L	probably benign	Het
Lrba	A	T	3: 86,325,022	M759L	possibly damaging	Het
Lrp8	A	G	4: 107,854,321	Y307C	probably damaging	Het
Neb	T	C	2: 52,147,156	H6847R	probably damaging	Het
Olfr599	T	C	7: 103,338,501	V149A	probably benign	Het
Olfr703	G	A	7: 106,844,695	C28Y	probably benign	Het
Pcdha5	C	A	18: 36,961,702	Y421*	probably null	Het
Pclo	T	A	5: 14,540,717	D1010E	unknown	Het
Pld2	A	T	11: 70,552,759	D449V	probably damaging	Het
Ppfia1	C	A	7: 144,485,095	M1032I	possibly damaging	Het
Prickle2	C	A	6: 92,376,210	L758F	probably damaging	Het
Sarm1	A	G	11: 78,483,476	V564A	probably damaging	Het
Taf1b	C	A	12: 24,549,438	F398L	probably benign	Het
Tll2	A	G	19: 41,117,257	I361T	possibly damaging	Het
Tmem260	T	C	14: 48,505,259	V517A	probably benign	Het
Usp24	A	G	4: 106,341,214	K106E	probably benign	Het
Vcan	C	A	13: 89,690,286	V2380F	probably benign	Het
Vmn2r65	A	G	7: 84,946,334	S381P	probably damaging	Het
Wdr5b	T	C	16: 36,041,832	V107A	probably benign	Het
Wdr7	G	T	18: 63,987,312	V1475F	probably benign	Het
Ythdc2	A	G	18: 44,860,621	D911G	probably damaging	Het
Zbtb32	T	A	7: 30,590,204	E11V	probably damaging	Het
Zfp563	T	G	17: 33,104,838	Y136D	probably damaging	Het

Other mutations in Vmn2r73

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00158:Vmn2r73	APN	7	85857587	missense	probably benign
IGL01151:Vmn2r73	APN	7	85857878	missense	probably damaging	0.99
IGL01408:Vmn2r73	APN	7	85872247	missense	probably benign	0.45
IGL01505:Vmn2r73	APN	7	85858059	nonsense	probably null
IGL01731:Vmn2r73	APN	7	85857549	makesense	probably null
IGL01818:Vmn2r73	APN	7	85869901	splice site	probably benign
IGL02236:Vmn2r73	APN	7	85872694	missense	probably benign	0.00
IGL02265:Vmn2r73	APN	7	85871639	missense	probably benign	0.02
IGL02266:Vmn2r73	APN	7	85875799	missense	possibly damaging	0.52
IGL02276:Vmn2r73	APN	7	85869772	critical splice donor site	probably null
IGL02284:Vmn2r73	APN	7	85857915	missense	possibly damaging	0.92
IGL02380:Vmn2r73	APN	7	85858175	missense	probably benign	0.00
IGL02466:Vmn2r73	APN	7	85872876	missense	probably damaging	1.00
IGL03172:Vmn2r73	APN	7	85858287	missense	probably benign	0.01
IGL03179:Vmn2r73	APN	7	85870260	missense	probably benign	0.04
G1Funyon:Vmn2r73	UTSW	7	85858302	missense	probably benign	0.00
R0077:Vmn2r73	UTSW	7	85875867	missense	probably benign	0.00
R0311:Vmn2r73	UTSW	7	85871789	missense	probably benign
R0413:Vmn2r73	UTSW	7	85871879	missense	possibly damaging	0.68
R0494:Vmn2r73	UTSW	7	85872932	missense	probably benign
R1523:Vmn2r73	UTSW	7	85870278	missense	probably benign	0.02
R1618:Vmn2r73	UTSW	7	85875912	nonsense	probably null
R1667:Vmn2r73	UTSW	7	85857681	missense	probably benign	0.00
R1728:Vmn2r73	UTSW	7	85857878	missense	probably damaging	1.00
R1729:Vmn2r73	UTSW	7	85857878	missense	probably damaging	1.00
R1747:Vmn2r73	UTSW	7	85858167	missense	probably damaging	1.00
R1784:Vmn2r73	UTSW	7	85857878	missense	probably damaging	1.00
R2152:Vmn2r73	UTSW	7	85857728	missense	probably benign	0.03
R2395:Vmn2r73	UTSW	7	85857767	missense	probably damaging	1.00
R2415:Vmn2r73	UTSW	7	85872223	missense	probably damaging	1.00
R2926:Vmn2r73	UTSW	7	85871663	missense	probably benign	0.17
R3719:Vmn2r73	UTSW	7	85870374	missense	probably damaging	1.00
R3766:Vmn2r73	UTSW	7	85871990	missense	probably benign
R3838:Vmn2r73	UTSW	7	85858050	missense	probably benign	0.00
R3890:Vmn2r73	UTSW	7	85857936	missense	probably benign
R4030:Vmn2r73	UTSW	7	85871836	missense	possibly damaging	0.92
R4247:Vmn2r73	UTSW	7	85872675	missense	probably damaging	0.99
R4409:Vmn2r73	UTSW	7	85871560	missense	probably damaging	0.97
R4427:Vmn2r73	UTSW	7	85857773	missense	probably damaging	1.00
R4430:Vmn2r73	UTSW	7	85870241	missense	probably benign
R4552:Vmn2r73	UTSW	7	85875847	missense	probably benign	0.12
R4779:Vmn2r73	UTSW	7	85871715	nonsense	probably null
R4906:Vmn2r73	UTSW	7	85857621	missense	probably damaging	0.99
R4942:Vmn2r73	UTSW	7	85870374	missense	probably damaging	1.00
R5344:Vmn2r73	UTSW	7	85875838	missense	probably benign	0.00
R5478:Vmn2r73	UTSW	7	85869788	missense	probably damaging	1.00
R5527:Vmn2r73	UTSW	7	85858367	missense	possibly damaging	0.65
R5691:Vmn2r73	UTSW	7	85858091	missense	probably damaging	1.00
R5826:Vmn2r73	UTSW	7	85875748	missense	possibly damaging	0.77
R6018:Vmn2r73	UTSW	7	85872667	missense	possibly damaging	0.48
R6082:Vmn2r73	UTSW	7	85858221	missense	probably benign	0.00
R6111:Vmn2r73	UTSW	7	85871789	missense	probably benign
R6233:Vmn2r73	UTSW	7	85869891	missense	probably benign	0.00
R6278:Vmn2r73	UTSW	7	85872932	missense	probably benign
R6283:Vmn2r73	UTSW	7	85871841	missense	probably benign	0.00
R6307:Vmn2r73	UTSW	7	85857620	missense	probably damaging	1.00
R6413:Vmn2r73	UTSW	7	85870336	nonsense	probably null
R6782:Vmn2r73	UTSW	7	85870355	missense	probably benign	0.00
R6884:Vmn2r73	UTSW	7	85858005	missense	probably benign	0.05
R6921:Vmn2r73	UTSW	7	85858238	missense	probably benign	0.27
R7169:Vmn2r73	UTSW	7	85858455	nonsense	probably null
R7206:Vmn2r73	UTSW	7	85872867	missense	probably benign	0.03
R7311:Vmn2r73	UTSW	7	85871984	missense	possibly damaging	0.80
R7650:Vmn2r73	UTSW	7	85871939	missense	probably benign
R7811:Vmn2r73	UTSW	7	85875748	missense	possibly damaging	0.77
R8263:Vmn2r73	UTSW	7	85858411	missense	probably benign
R8301:Vmn2r73	UTSW	7	85858302	missense	probably benign	0.00
R8341:Vmn2r73	UTSW	7	85857920	missense	probably benign	0.01
R8500:Vmn2r73	UTSW	7	85870430	missense	probably benign	0.01
R8673:Vmn2r73	UTSW	7	85872694	missense	probably benign	0.01
R8719:Vmn2r73	UTSW	7	85871887	missense	probably damaging	0.96
R8783:Vmn2r73	UTSW	7	85858460	missense	probably damaging	0.96
R8828:Vmn2r73	UTSW	7	85871971	missense	probably damaging	0.98
X0023:Vmn2r73	UTSW	7	85858456	missense	probably benign	0.31
Z1176:Vmn2r73	UTSW	7	85871968	missense	probably damaging	0.99
Z1177:Vmn2r73	UTSW	7	85872272	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AATGTCAAGAGCTTGGCTTCATTG -3'
(R):5'- TCATCTGTGTCCTGCAGCAG -3'

Sequencing Primer
(F):5'- GCTTCATTGAATGTATCAGGCAG -3'
(R):5'- TCCTGCAGCAGAGCACATTTG -3'

Posted On

2016-07-22