Incidental Mutation 'R5302:Nid2'
ID 404317
Institutional Source Beutler Lab
Gene Symbol Nid2
Ensembl Gene ENSMUSG00000021806
Gene Name nidogen 2
Synonyms entactin 2, entactin-2
MMRRC Submission 042885-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.164) question?
Stock # R5302 (G1)
Quality Score 225
Status Not validated
Chromosome 14
Chromosomal Location 19801333-19861855 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to G at 19829769 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Threonine to Alanine at position 687 (T687A)
Ref Sequence ENSEMBL: ENSMUSP00000022340 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000022340]
AlphaFold O88322
Predicted Effect probably benign
Transcript: ENSMUST00000022340
AA Change: T687A

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
SMART Domains Protein: ENSMUSP00000022340
Gene: ENSMUSG00000021806
AA Change: T687A

DomainStartEndE-ValueType
signal peptide 1 30 N/A INTRINSIC
Blast:NIDO 39 77 3e-11 BLAST
NIDO 108 276 1.12e-72 SMART
low complexity region 421 430 N/A INTRINSIC
low complexity region 452 470 N/A INTRINSIC
EGF 510 547 1.84e1 SMART
G2F 548 780 4.36e-143 SMART
EGF 785 823 2.52e-2 SMART
EGF_CA 824 866 1.45e-11 SMART
EGF 874 914 3.15e-3 SMART
EGF_CA 915 953 5.03e-11 SMART
TY 988 1037 8.27e-20 SMART
TY 1068 1116 1.19e-20 SMART
LY 1162 1204 1.15e-5 SMART
LY 1206 1248 8.82e-16 SMART
LY 1249 1293 1.51e-14 SMART
LY 1294 1336 3.56e-11 SMART
Predicted Effect unknown
Transcript: ENSMUST00000224263
AA Change: T421A
Predicted Effect noncoding transcript
Transcript: ENSMUST00000225791
Coding Region Coverage
  • 1x: 99.4%
  • 3x: 98.8%
  • 10x: 97.6%
  • 20x: 96.0%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the nidogen family of basement membrane proteins. This protein is a cell-adhesion protein that binds collagens I and IV and laminin and may be involved in maintaining the structure of the basement membrane.[provided by RefSeq, Jun 2010]
PHENOTYPE: Mice homozygous for a null alleleexhibit calcification of joint cartilage and osteoarthritis. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 41 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abca12 A G 1: 71,323,111 (GRCm39) V1657A possibly damaging Het
Abca8b C A 11: 109,868,639 (GRCm39) G175V probably damaging Het
Acot5 A G 12: 84,120,215 (GRCm39) Y190C probably damaging Het
Ascc3 T A 10: 50,583,873 (GRCm39) Y941N probably benign Het
BC035947 A T 1: 78,488,599 (GRCm39) M1K probably null Het
C2cd5 A G 6: 143,019,482 (GRCm39) C278R probably benign Het
Cd200r1 G T 16: 44,613,172 (GRCm39) L259F possibly damaging Het
Clcn4 A G 7: 7,297,050 (GRCm39) V136A possibly damaging Het
Cntnap5a T C 1: 116,085,300 (GRCm39) S413P probably benign Het
Corin T A 5: 72,473,441 (GRCm39) E748D probably benign Het
Crtc2 G T 3: 90,168,325 (GRCm39) G356V probably damaging Het
D1Pas1 T A 1: 186,701,642 (GRCm39) Y524N probably damaging Het
Dlgap4 T C 2: 156,602,818 (GRCm39) S147P probably damaging Het
Eif1ad16 T A 12: 87,985,316 (GRCm39) I76F probably damaging Het
Enpp1 A T 10: 24,527,288 (GRCm39) I633N probably benign Het
Gm3095 A G 14: 15,170,367 (GRCm39) D72G probably null Het
Gm5449 C T 13: 53,679,787 (GRCm39) noncoding transcript Het
Gpx7 T C 4: 108,258,111 (GRCm39) T161A probably benign Het
Grip1 T C 10: 119,855,982 (GRCm39) L236P probably damaging Het
H2-Q2 G T 17: 35,563,885 (GRCm39) R255S probably damaging Het
Il17rc G T 6: 113,459,997 (GRCm39) A648S possibly damaging Het
Klhl12 A G 1: 134,417,189 (GRCm39) E540G possibly damaging Het
Mcm10 T C 2: 5,012,181 (GRCm39) I135V probably benign Het
Mrps26 C A 2: 130,406,087 (GRCm39) T100K probably benign Het
Npas3 A T 12: 54,115,619 (GRCm39) D829V probably damaging Het
Ocln T C 13: 100,642,807 (GRCm39) D176G probably damaging Het
Or11h6 A G 14: 50,879,776 (GRCm39) probably null Het
Pax3 A C 1: 78,098,249 (GRCm39) M380R possibly damaging Het
Pcdha3 A G 18: 37,081,208 (GRCm39) E650G probably damaging Het
Pdcd11 C A 19: 47,096,083 (GRCm39) H668N probably damaging Het
Polr3b T C 10: 84,535,264 (GRCm39) Y858H possibly damaging Het
Pus10 T C 11: 23,617,416 (GRCm39) probably null Het
Raver1 T C 9: 20,986,677 (GRCm39) D739G probably damaging Het
Skic3 G A 13: 76,295,886 (GRCm39) E1050K possibly damaging Het
Slc26a11 T C 11: 119,254,276 (GRCm39) L198P probably damaging Het
Slc44a3 A G 3: 121,303,962 (GRCm39) V258A probably damaging Het
Socs7 T A 11: 97,280,025 (GRCm39) I524N probably damaging Het
Steap4 T A 5: 8,025,547 (GRCm39) L36* probably null Het
Svep1 G C 4: 58,096,183 (GRCm39) T1479S possibly damaging Het
Ttn C A 2: 76,547,619 (GRCm39) V32184F probably damaging Het
Vmn1r22 T C 6: 57,877,960 (GRCm39) N6D possibly damaging Het
Other mutations in Nid2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01783:Nid2 APN 14 19,818,745 (GRCm39) missense probably benign
IGL01788:Nid2 APN 14 19,858,047 (GRCm39) missense probably damaging 1.00
IGL02259:Nid2 APN 14 19,818,277 (GRCm39) critical splice acceptor site probably null
IGL03084:Nid2 APN 14 19,819,000 (GRCm39) missense probably benign 0.05
IGL03247:Nid2 APN 14 19,829,688 (GRCm39) missense probably damaging 1.00
IGL03098:Nid2 UTSW 14 19,856,006 (GRCm39) missense probably damaging 0.99
PIT4810001:Nid2 UTSW 14 19,860,158 (GRCm39) missense possibly damaging 0.82
R0173:Nid2 UTSW 14 19,852,400 (GRCm39) splice site probably benign
R0501:Nid2 UTSW 14 19,839,736 (GRCm39) splice site probably null
R1117:Nid2 UTSW 14 19,813,732 (GRCm39) critical splice acceptor site probably null
R1305:Nid2 UTSW 14 19,818,930 (GRCm39) missense probably benign 0.00
R1572:Nid2 UTSW 14 19,855,480 (GRCm39) missense probably benign 0.08
R1594:Nid2 UTSW 14 19,831,329 (GRCm39) missense probably benign 0.03
R1789:Nid2 UTSW 14 19,802,499 (GRCm39) missense possibly damaging 0.95
R1927:Nid2 UTSW 14 19,818,344 (GRCm39) missense probably damaging 1.00
R2085:Nid2 UTSW 14 19,828,111 (GRCm39) missense probably benign 0.12
R2086:Nid2 UTSW 14 19,828,111 (GRCm39) missense probably benign 0.12
R2100:Nid2 UTSW 14 19,828,946 (GRCm39) nonsense probably null
R2158:Nid2 UTSW 14 19,828,111 (GRCm39) missense probably benign 0.12
R2240:Nid2 UTSW 14 19,855,982 (GRCm39) missense probably damaging 0.99
R2314:Nid2 UTSW 14 19,839,829 (GRCm39) missense probably benign 0.01
R2863:Nid2 UTSW 14 19,818,471 (GRCm39) missense possibly damaging 0.92
R3113:Nid2 UTSW 14 19,828,111 (GRCm39) missense probably benign 0.12
R3545:Nid2 UTSW 14 19,813,779 (GRCm39) missense probably damaging 1.00
R3548:Nid2 UTSW 14 19,813,779 (GRCm39) missense probably damaging 1.00
R3801:Nid2 UTSW 14 19,860,065 (GRCm39) missense probably damaging 1.00
R4618:Nid2 UTSW 14 19,858,078 (GRCm39) missense probably damaging 0.99
R4798:Nid2 UTSW 14 19,839,829 (GRCm39) missense probably benign 0.01
R4953:Nid2 UTSW 14 19,828,146 (GRCm39) nonsense probably null
R5256:Nid2 UTSW 14 19,818,276 (GRCm39) critical splice acceptor site probably null
R5289:Nid2 UTSW 14 19,855,379 (GRCm39) missense possibly damaging 0.95
R5409:Nid2 UTSW 14 19,856,030 (GRCm39) missense probably damaging 1.00
R5514:Nid2 UTSW 14 19,852,535 (GRCm39) missense probably damaging 1.00
R6134:Nid2 UTSW 14 19,828,851 (GRCm39) missense probably damaging 1.00
R6365:Nid2 UTSW 14 19,853,201 (GRCm39) missense probably damaging 1.00
R6647:Nid2 UTSW 14 19,852,484 (GRCm39) missense probably benign 0.04
R6758:Nid2 UTSW 14 19,852,551 (GRCm39) missense probably damaging 1.00
R6882:Nid2 UTSW 14 19,839,775 (GRCm39) missense probably damaging 1.00
R6893:Nid2 UTSW 14 19,839,855 (GRCm39) missense probably benign 0.34
R7045:Nid2 UTSW 14 19,829,749 (GRCm39) missense possibly damaging 0.94
R7392:Nid2 UTSW 14 19,818,724 (GRCm39) missense probably benign 0.00
R7477:Nid2 UTSW 14 19,856,041 (GRCm39) missense probably benign 0.09
R7515:Nid2 UTSW 14 19,841,635 (GRCm39) missense probably benign 0.06
R7547:Nid2 UTSW 14 19,847,345 (GRCm39) missense probably benign
R7594:Nid2 UTSW 14 19,818,791 (GRCm39) missense probably benign 0.00
R7615:Nid2 UTSW 14 19,852,598 (GRCm39) missense probably damaging 1.00
R7680:Nid2 UTSW 14 19,829,715 (GRCm39) missense probably damaging 1.00
R8097:Nid2 UTSW 14 19,848,657 (GRCm39) missense possibly damaging 0.77
R8167:Nid2 UTSW 14 19,860,131 (GRCm39) missense possibly damaging 0.57
R8292:Nid2 UTSW 14 19,818,346 (GRCm39) missense probably damaging 0.99
R8766:Nid2 UTSW 14 19,802,340 (GRCm39) missense probably benign 0.26
R9093:Nid2 UTSW 14 19,858,009 (GRCm39) missense
R9193:Nid2 UTSW 14 19,853,278 (GRCm39) missense probably damaging 1.00
RF002:Nid2 UTSW 14 19,801,434 (GRCm39) small deletion probably benign
RF016:Nid2 UTSW 14 19,801,431 (GRCm39) small deletion probably benign
X0009:Nid2 UTSW 14 19,852,579 (GRCm39) missense probably damaging 1.00
X0021:Nid2 UTSW 14 19,818,930 (GRCm39) missense probably benign 0.00
X0026:Nid2 UTSW 14 19,828,199 (GRCm39) missense probably damaging 0.96
Z1177:Nid2 UTSW 14 19,839,876 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- GCTGAGTTTCTGTCTCCTGCAG -3'
(R):5'- AGCACTCTGATCACCTGTATAC -3'

Sequencing Primer
(F):5'- GGTGCTACCTTTGTCCATGACG -3'
(R):5'- GTGTATACGTCCTAAAAACAAGAACG -3'
Posted On 2016-07-22