Incidental Mutation 'R5305:Zfp574'
ID404503
Institutional Source Beutler Lab
Gene Symbol Zfp574
Ensembl Gene ENSMUSG00000045252
Gene Namezinc finger protein 574
SynonymsA630056B21Rik
MMRRC Submission 042888-MU
Accession Numbers
Is this an essential gene? Probably essential (E-score: 0.958) question?
Stock #R5305 (G1)
Quality Score225
Status Validated
Chromosome7
Chromosomal Location25072567-25083492 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) T to A at 25081090 bp
ZygosityHeterozygous
Amino Acid Change Histidine to Glutamine at position 512 (H512Q)
Ref Sequence ENSEMBL: ENSMUSP00000136547 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000053410] [ENSMUST00000179556]
Predicted Effect probably damaging
Transcript: ENSMUST00000053410
AA Change: H512Q

PolyPhen 2 Score 0.975 (Sensitivity: 0.76; Specificity: 0.96)
SMART Domains Protein: ENSMUSP00000057817
Gene: ENSMUSG00000045252
AA Change: H512Q

DomainStartEndE-ValueType
ZnF_C2H2 16 38 4.98e-1 SMART
ZnF_C2H2 76 98 4.05e-1 SMART
low complexity region 107 118 N/A INTRINSIC
ZnF_C2H2 126 148 1.99e0 SMART
low complexity region 156 173 N/A INTRINSIC
ZnF_C2H2 213 235 4.4e-2 SMART
low complexity region 237 254 N/A INTRINSIC
low complexity region 289 298 N/A INTRINSIC
ZnF_C2H2 312 334 7.15e-2 SMART
ZnF_C2H2 339 361 1.04e-3 SMART
ZnF_C2H2 367 389 4.12e0 SMART
ZnF_C2H2 395 416 2.12e1 SMART
low complexity region 418 434 N/A INTRINSIC
ZnF_C2H2 469 492 1.03e-2 SMART
ZnF_C2H2 498 520 2.75e-3 SMART
ZnF_C2H2 526 548 3.39e-3 SMART
ZnF_C2H2 554 576 2.75e-3 SMART
ZnF_C2H2 582 604 2.86e-1 SMART
ZnF_C2H2 610 633 1.25e-1 SMART
ZnF_C2H2 639 659 4.5e1 SMART
low complexity region 660 666 N/A INTRINSIC
ZnF_C2H2 670 692 2.29e0 SMART
ZnF_C2H2 742 764 2.91e-2 SMART
ZnF_C2H2 770 792 5.59e-4 SMART
ZnF_C2H2 798 820 3.34e-2 SMART
ZnF_C2H2 826 848 4.24e-4 SMART
Predicted Effect probably damaging
Transcript: ENSMUST00000179556
AA Change: H512Q

PolyPhen 2 Score 0.975 (Sensitivity: 0.76; Specificity: 0.96)
SMART Domains Protein: ENSMUSP00000136547
Gene: ENSMUSG00000045252
AA Change: H512Q

DomainStartEndE-ValueType
ZnF_C2H2 16 38 4.98e-1 SMART
ZnF_C2H2 76 98 4.05e-1 SMART
low complexity region 107 118 N/A INTRINSIC
ZnF_C2H2 126 148 1.99e0 SMART
low complexity region 156 173 N/A INTRINSIC
ZnF_C2H2 213 235 4.4e-2 SMART
low complexity region 237 254 N/A INTRINSIC
low complexity region 289 298 N/A INTRINSIC
ZnF_C2H2 312 334 7.15e-2 SMART
ZnF_C2H2 339 361 1.04e-3 SMART
ZnF_C2H2 367 389 4.12e0 SMART
ZnF_C2H2 395 416 2.12e1 SMART
low complexity region 418 434 N/A INTRINSIC
ZnF_C2H2 469 492 1.03e-2 SMART
ZnF_C2H2 498 520 2.75e-3 SMART
ZnF_C2H2 526 548 3.39e-3 SMART
ZnF_C2H2 554 576 2.75e-3 SMART
ZnF_C2H2 582 604 2.86e-1 SMART
ZnF_C2H2 610 633 1.25e-1 SMART
ZnF_C2H2 639 659 4.5e1 SMART
low complexity region 660 666 N/A INTRINSIC
ZnF_C2H2 670 692 2.29e0 SMART
ZnF_C2H2 742 764 2.91e-2 SMART
ZnF_C2H2 770 792 5.59e-4 SMART
ZnF_C2H2 798 820 3.34e-2 SMART
ZnF_C2H2 826 848 4.24e-4 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000206530
Predicted Effect noncoding transcript
Transcript: ENSMUST00000206733
Meta Mutation Damage Score 0.6467 question?
Coding Region Coverage
  • 1x: 99.3%
  • 3x: 98.7%
  • 10x: 97.4%
  • 20x: 95.4%
Validation Efficiency 99% (67/68)
Allele List at MGI
Other mutations in this stock
Total: 57 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4930407I10Rik G A 15: 82,059,219 V11M possibly damaging Het
Acot8 A G 2: 164,795,765 V179A probably benign Het
Actb A G 5: 142,904,230 I194T probably benign Het
Ap1g2 A G 14: 55,099,076 V787A probably benign Het
Areg G T 5: 91,144,449 A203S probably damaging Het
Asb13 A T 13: 3,643,479 D79V probably damaging Het
Atad2b C T 12: 4,965,855 T527I probably damaging Het
Auts2 T G 5: 131,443,794 probably benign Het
Ceacam3 T A 7: 17,151,576 S35T probably damaging Het
Crebzf T C 7: 90,444,134 probably benign Het
Cttnbp2 G T 6: 18,381,098 N1366K probably benign Het
Cubn C A 2: 13,388,939 C1417F probably damaging Het
Dot1l C T 10: 80,790,793 P162S probably benign Het
Epc1 G A 18: 6,490,690 probably benign Het
Eps8l1 A G 7: 4,477,896 S613G possibly damaging Het
Erich6 T G 3: 58,625,116 I357L probably benign Het
Foxj3 T A 4: 119,619,958 S288T possibly damaging Het
Gls2 T G 10: 128,204,709 Y326* probably null Het
Gm11595 G A 11: 99,772,555 R100C unknown Het
Gm7535 T C 17: 17,911,799 probably benign Het
Gxylt2 T G 6: 100,787,218 L288R probably damaging Het
Kdm4a T C 4: 118,160,501 Y456C probably damaging Het
Mgat4c T A 10: 102,389,279 F451L possibly damaging Het
Mrpl20 G A 4: 155,803,705 R17H probably damaging Het
Mtf2 A G 5: 108,104,499 T465A possibly damaging Het
Mycbp2 A C 14: 103,346,321 L66R probably benign Het
Nos1ap T A 1: 170,349,399 K145M probably damaging Het
Nr2e1 A T 10: 42,571,487 Y176* probably null Het
Obscn T C 11: 59,012,715 T7628A possibly damaging Het
Olfr1353 A T 10: 78,970,556 K302N possibly damaging Het
Pitx2 T G 3: 129,215,840 V129G probably damaging Het
Polr2e A G 10: 80,038,063 probably benign Het
Ppard A G 17: 28,298,858 D300G probably damaging Het
Ppp1r27 A G 11: 120,550,917 V46A probably benign Het
Prex2 T A 1: 11,107,678 V332E probably damaging Het
Prss30 T G 17: 23,972,776 Y257S probably benign Het
Ptprd T C 4: 75,982,626 E1082G probably damaging Het
Rab3c T A 13: 110,181,077 R89S probably damaging Het
Rimbp2 A G 5: 128,797,381 V389A possibly damaging Het
Rims1 T A 1: 22,596,542 R119S probably damaging Het
Sema3b T C 9: 107,603,337 H137R probably null Het
Sf3b4 G C 3: 96,173,642 A89P probably damaging Het
Sgta T A 10: 81,046,247 Q298L probably damaging Het
Spag9 C A 11: 94,069,012 D342E probably damaging Het
Sry T A Y: 2,662,982 D226V unknown Het
Sv2a A G 3: 96,185,458 E158G possibly damaging Het
Sytl2 A G 7: 90,381,863 probably benign Het
Thbs3 T A 3: 89,217,976 probably benign Het
Top3a A T 11: 60,762,539 N56K possibly damaging Het
Ttc37 G A 13: 76,147,767 E1050K possibly damaging Het
Tyk2 T C 9: 21,109,381 D918G probably damaging Het
Uqcrh A G 4: 116,067,284 probably benign Het
Vmn1r61 A G 7: 5,610,815 S167P probably damaging Het
Wdr25 C A 12: 109,026,440 H74N probably damaging Het
Zfp458 T C 13: 67,256,318 N686D probably benign Het
Zfp976 A T 7: 42,613,478 Y312N probably benign Het
Zscan4c G A 7: 11,009,535 V354I probably benign Het
Other mutations in Zfp574
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00228:Zfp574 APN 7 25081590 missense probably benign 0.00
IGL02370:Zfp574 APN 7 25079589 missense possibly damaging 0.95
IGL02706:Zfp574 APN 7 25081365 missense probably damaging 0.98
IGL03119:Zfp574 APN 7 25080473 missense probably benign
glue UTSW 7 25081090 missense probably damaging 0.98
R0866:Zfp574 UTSW 7 25079898 missense probably damaging 1.00
R2429:Zfp574 UTSW 7 25080057 nonsense probably null
R3123:Zfp574 UTSW 7 25081601 missense possibly damaging 0.88
R3124:Zfp574 UTSW 7 25081601 missense possibly damaging 0.88
R3125:Zfp574 UTSW 7 25081601 missense possibly damaging 0.88
R4581:Zfp574 UTSW 7 25081313 missense probably damaging 0.98
R4591:Zfp574 UTSW 7 25079544 start gained probably benign
R4915:Zfp574 UTSW 7 25080726 missense probably damaging 0.98
R4953:Zfp574 UTSW 7 25080963 missense probably damaging 0.97
R5541:Zfp574 UTSW 7 25081950 missense probably damaging 0.99
R5934:Zfp574 UTSW 7 25080332 missense probably benign
R6088:Zfp574 UTSW 7 25080339 missense probably benign 0.01
R7061:Zfp574 UTSW 7 25080197 missense possibly damaging 0.95
R7563:Zfp574 UTSW 7 25081352 missense possibly damaging 0.94
R7615:Zfp574 UTSW 7 25080576 missense possibly damaging 0.95
R8019:Zfp574 UTSW 7 25080670 nonsense probably null
X0026:Zfp574 UTSW 7 25081052 missense probably damaging 0.99
Predicted Primers PCR Primer
(F):5'- TATGTGAGGAGAGTTCAGCCG -3'
(R):5'- ATCACAAGCTGGTGGACCTC -3'

Sequencing Primer
(F):5'- AGAGTTCAGCCGAGCCTG -3'
(R):5'- ACGGCACTTGTAGGGGTACTC -3'
Posted On2016-07-22