Incidental Mutation 'R5306:Gpr3'
ID 404548
Institutional Source Beutler Lab
Gene Symbol Gpr3
Ensembl Gene ENSMUSG00000049649
Gene Name G-protein coupled receptor 3
Synonyms Gpcr3, Gpcr21
MMRRC Submission 042889-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.185) question?
Stock # R5306 (G1)
Quality Score 217
Status Validated
Chromosome 4
Chromosomal Location 133209340-133212536 bp(-) (GRCm38)
Type of Mutation missense
DNA Base Change (assembly) C to T at 133211179 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Valine to Methionine at position 61 (V61M)
Ref Sequence ENSEMBL: ENSMUSP00000116032 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000052090] [ENSMUST00000105911] [ENSMUST00000151025]
AlphaFold P35413
Predicted Effect probably damaging
Transcript: ENSMUST00000052090
AA Change: V61M

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000062083
Gene: ENSMUSG00000049649
AA Change: V61M

low complexity region 13 24 N/A INTRINSIC
Pfam:7tm_1 57 297 2.4e-40 PFAM
Predicted Effect probably damaging
Transcript: ENSMUST00000105911
AA Change: V61M

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000101531
Gene: ENSMUSG00000049649
AA Change: V61M

low complexity region 13 24 N/A INTRINSIC
Pfam:7tm_1 57 297 2e-34 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000150744
Predicted Effect probably damaging
Transcript: ENSMUST00000151025
AA Change: V61M

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000116032
Gene: ENSMUSG00000049649
AA Change: V61M

SCOP:d1l9ha_ 20 95 3e-3 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000218338
Predicted Effect noncoding transcript
Transcript: ENSMUST00000218998
Meta Mutation Damage Score 0.8206 question?
Coding Region Coverage
  • 1x: 99.3%
  • 3x: 98.7%
  • 10x: 97.3%
  • 20x: 95.2%
Validation Efficiency 97% (64/66)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is a member of the G protein-coupled receptor family and is found in the cell membrane. G protein-coupled receptors, characterized by a seven transmembrane domain motif, are involved in translating outside signals into G protein mediated intracellular effects. The encoded protein activates adenylate cyclase and modulates amyloid-beta production in a mouse model, suggesting that it may play a role in Alzheimer's disease. [provided by RefSeq, Oct 2012]
PHENOTYPE: Mice homozygous for one allele display an increased thermal nociceptive threshold while females homozygous for another allele display age-related reproductive failure due to ovulation of a progressively increasing proportion of developmentally incompetent oocytes. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 62 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Adam29 T A 8: 55,871,757 (GRCm38) D554V probably damaging Het
Ankrd44 G A 1: 54,926,203 (GRCm38) probably benign Het
Api5 A T 2: 94,423,466 (GRCm38) C297* probably null Het
Asb14 G A 14: 26,911,909 (GRCm38) C357Y probably damaging Het
Brd10 A G 19: 29,729,830 (GRCm38) probably benign Het
Brdt T A 5: 107,345,144 (GRCm38) D112E probably damaging Het
Capsl C A 15: 9,457,790 (GRCm38) Q32K probably benign Het
Ccdc106 A G 7: 5,058,097 (GRCm38) D81G probably damaging Het
Ccdc121rt3 T C 5: 112,355,044 (GRCm38) R265G probably benign Het
Cep104 C A 4: 154,006,242 (GRCm38) T884K probably benign Het
Cmbl T C 15: 31,582,069 (GRCm38) Y71H probably damaging Het
Crybg3 A T 16: 59,559,993 (GRCm38) probably benign Het
Dynlt1c T C 17: 6,601,811 (GRCm38) M1T probably null Het
Erbb2 T C 11: 98,428,206 (GRCm38) S574P probably benign Het
Exosc10 T C 4: 148,562,392 (GRCm38) V153A probably benign Het
Faxc G T 4: 21,931,557 (GRCm38) probably benign Het
Fcgbp A G 7: 28,091,818 (GRCm38) T835A probably damaging Het
Fmo5 G T 3: 97,641,760 (GRCm38) M241I probably benign Het
Gabra1 A C 11: 42,133,552 (GRCm38) I432S probably benign Het
Gfap A G 11: 102,895,748 (GRCm38) probably null Het
Gm11595 G A 11: 99,772,555 (GRCm38) R100C unknown Het
Gm12185 T A 11: 48,915,555 (GRCm38) M270L probably benign Het
Gm14443 T C 2: 175,169,579 (GRCm38) N358S possibly damaging Het
Herc2 C T 7: 56,184,961 (GRCm38) T3229M probably damaging Het
Ifit3 A G 19: 34,587,807 (GRCm38) Y251C probably damaging Het
Inf2 G A 12: 112,601,553 (GRCm38) V180I probably benign Het
Ints11 T C 4: 155,875,208 (GRCm38) Y91H probably damaging Het
Ints4 A C 7: 97,509,678 (GRCm38) D419A probably damaging Het
Kmt2e T C 5: 23,499,333 (GRCm38) S1175P probably damaging Het
Mki67 A T 7: 135,714,001 (GRCm38) V44E probably damaging Het
Mrgprb13 A T 7: 48,312,192 (GRCm38) noncoding transcript Het
Myh2 T C 11: 67,186,556 (GRCm38) L839P probably damaging Het
Nos1ap T A 1: 170,349,399 (GRCm38) K145M probably damaging Het
Or4l1 A G 14: 49,929,550 (GRCm38) probably benign Het
Or6c70 A C 10: 129,873,941 (GRCm38) I272R probably damaging Het
Pced1a T A 2: 130,419,171 (GRCm38) H422L probably benign Het
Plpp1 G T 13: 112,851,555 (GRCm38) probably null Het
Plxna4 T C 6: 32,206,121 (GRCm38) Y949C probably damaging Het
Polg2 G A 11: 106,778,970 (GRCm38) T158I probably damaging Het
Prss38 A T 11: 59,372,995 (GRCm38) I297K probably benign Het
Psph A G 5: 129,769,367 (GRCm38) L98P probably damaging Het
Rab11b G A 17: 33,760,269 (GRCm38) probably benign Het
Rsf1 CGGCGGC CGGCGGCGGGGGCGGC 7: 97,579,929 (GRCm38) probably benign Het
Serpine3 G A 14: 62,670,933 (GRCm38) A137T probably damaging Het
Sh3bp4 T C 1: 89,144,275 (GRCm38) F282L probably damaging Het
Sh3bp5 C A 14: 31,377,495 (GRCm38) R265L probably benign Het
Skic3 G A 13: 76,147,767 (GRCm38) E1050K possibly damaging Het
Slco2b1 T A 7: 99,688,991 (GRCm38) Y109F possibly damaging Het
Slfn10-ps T A 11: 83,035,529 (GRCm38) noncoding transcript Het
Smc5 A G 19: 23,259,645 (GRCm38) probably null Het
Smyd4 A G 11: 75,402,158 (GRCm38) N638S probably benign Het
Stxbp5 T C 10: 9,799,991 (GRCm38) E628G probably damaging Het
Tmem236 A T 2: 14,219,164 (GRCm38) K255* probably null Het
Ttc29 T A 8: 78,251,910 (GRCm38) probably null Het
Tyr A G 7: 87,438,014 (GRCm38) I430T probably damaging Het
Uckl1 A G 2: 181,574,367 (GRCm38) probably null Het
Vmn2r52 A C 7: 10,170,745 (GRCm38) I389R possibly damaging Het
Wdr62 A T 7: 30,265,263 (GRCm38) F352Y possibly damaging Het
Wdr70 T C 15: 7,924,273 (GRCm38) D379G probably benign Het
Zfp408 T A 2: 91,646,345 (GRCm38) M155L probably benign Het
Zfp459 T A 13: 67,413,130 (GRCm38) Q66H probably damaging Het
Zfp870 C A 17: 32,883,653 (GRCm38) G234V probably damaging Het
Other mutations in Gpr3
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02424:Gpr3 APN 4 133,211,094 (GRCm38) missense probably damaging 1.00
IGL02974:Gpr3 APN 4 133,210,909 (GRCm38) missense possibly damaging 0.90
R0095:Gpr3 UTSW 4 133,211,286 (GRCm38) missense probably benign 0.01
R0532:Gpr3 UTSW 4 133,210,485 (GRCm38) missense probably damaging 1.00
R1831:Gpr3 UTSW 4 133,211,143 (GRCm38) missense possibly damaging 0.80
R2127:Gpr3 UTSW 4 133,210,621 (GRCm38) missense probably damaging 0.96
R4077:Gpr3 UTSW 4 133,210,915 (GRCm38) missense probably damaging 1.00
R4078:Gpr3 UTSW 4 133,210,915 (GRCm38) missense probably damaging 1.00
R5602:Gpr3 UTSW 4 133,210,494 (GRCm38) missense probably damaging 1.00
R5712:Gpr3 UTSW 4 133,210,408 (GRCm38) missense probably benign 0.01
R5913:Gpr3 UTSW 4 133,211,178 (GRCm38) missense probably damaging 1.00
R7434:Gpr3 UTSW 4 133,211,137 (GRCm38) missense probably benign 0.45
R7645:Gpr3 UTSW 4 133,211,329 (GRCm38) missense probably damaging 1.00
R7709:Gpr3 UTSW 4 133,210,437 (GRCm38) missense probably damaging 1.00
R8043:Gpr3 UTSW 4 133,210,960 (GRCm38) missense probably damaging 1.00
R8818:Gpr3 UTSW 4 133,211,227 (GRCm38) missense possibly damaging 0.95
R9015:Gpr3 UTSW 4 133,211,079 (GRCm38) missense possibly damaging 0.80
R9027:Gpr3 UTSW 4 133,210,898 (GRCm38) nonsense probably null
R9587:Gpr3 UTSW 4 133,210,677 (GRCm38) missense probably damaging 1.00
Predicted Primers PCR Primer

Sequencing Primer
Posted On 2016-07-22