Mutagenetix > Incidental Mutation

Incidental Mutation 'R5307:Abca8b'

404661

Institutional Source

Beutler Lab

Gene Symbol

Abca8b

Ensembl Gene

ENSMUSG00000020620

Gene Name

ATP-binding cassette, sub-family A (ABC1), member 8b

Synonyms

Abca8

MMRRC Submission

042890-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5307 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

109932190-109995845 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 109977813 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Valine at position 175 (G175V)

Ref Sequence

ENSEMBL: ENSMUSP00000102280 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000020948] [ENSMUST00000106669]

AlphaFold

Q8K440

Predicted Effect

probably damaging
Transcript: ENSMUST00000020948
AA Change: G175V

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000020948
Gene: ENSMUSG00000020620
AA Change: G175V

Domain	Start	End	E-Value	Type
Pfam:ABC2_membrane_3	28	417	3.9e-28	PFAM
AAA	507	691	6.36e-10	SMART
Pfam:ABC2_membrane_3	859	1215	1e-10	PFAM
low complexity region	1246	1255	N/A	INTRINSIC
AAA	1313	1492	6.17e-8	SMART
low complexity region	1597	1607	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000106669
AA Change: G175V

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000102280
Gene: ENSMUSG00000020620
AA Change: G175V

Domain	Start	End	E-Value	Type
Pfam:ABC2_membrane_3	28	344	2.6e-16	PFAM
AAA	445	629	6.36e-10	SMART
transmembrane domain	798	815	N/A	INTRINSIC
transmembrane domain	1001	1023	N/A	INTRINSIC
transmembrane domain	1038	1060	N/A	INTRINSIC
transmembrane domain	1072	1091	N/A	INTRINSIC
transmembrane domain	1101	1123	N/A	INTRINSIC
transmembrane domain	1136	1158	N/A	INTRINSIC
low complexity region	1184	1193	N/A	INTRINSIC
AAA	1251	1430	6.17e-8	SMART
low complexity region	1535	1545	N/A	INTRINSIC

Meta Mutation Damage Score

0.9202

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 97.2%
20x: 95.1%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. The encoded protein may regulate lipid metabolism and be involved in the formation and maintenance of myelin. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Allele List at MGI

Other mutations in this stock

Total: 78 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700006A11Rik	C	G	3: 124,406,350 (GRCm38)	G531A	probably damaging	Het
2410089E03Rik	G	A	15: 8,260,690 (GRCm38)		probably null	Het
Ank3	G	A	10: 70,002,565 (GRCm38)	R1566K	possibly damaging	Het
Ap3d1	T	C	10: 80,723,549 (GRCm38)	T264A	probably benign	Het
Arhgef17	C	G	7: 100,929,428 (GRCm38)	G771A	probably benign	Het
Atg2b	T	A	12: 105,658,329 (GRCm38)	D637V	probably benign	Het
Atp10b	A	G	11: 43,212,475 (GRCm38)	E562G	probably damaging	Het
Atp1a1	A	G	3: 101,589,964 (GRCm38)	V342A	probably damaging	Het
Atp2a2	A	G	5: 122,461,747 (GRCm38)	I527T	probably benign	Het
Atr	T	A	9: 95,878,544 (GRCm38)	N1022K	probably benign	Het
Bach2	T	A	4: 32,562,683 (GRCm38)	D383E	probably benign	Het
Casq1	A	T	1: 172,219,416 (GRCm38)	L92Q	probably damaging	Het
Chd1	T	A	17: 15,732,570 (GRCm38)	Y371N	probably damaging	Het
Chd9	G	A	8: 90,997,149 (GRCm38)	A617T	probably damaging	Het
Cntrob	T	A	11: 69,314,750 (GRCm38)	R419S	possibly damaging	Het
Corin	C	A	5: 72,356,978 (GRCm38)	G318C	probably damaging	Het
Cpa3	A	G	3: 20,227,163 (GRCm38)		probably null	Het
Crybg1	T	C	10: 44,003,714 (GRCm38)	S493G	probably benign	Het
Ddc	A	G	11: 11,876,321 (GRCm38)	F80S	probably damaging	Het
Dhrs2	A	G	14: 55,236,144 (GRCm38)	S87G	possibly damaging	Het
Dnah12	A	G	14: 26,693,486 (GRCm38)	E14G	possibly damaging	Het
Dtd1	A	G	2: 144,747,022 (GRCm38)	E200G	possibly damaging	Het
Dync2h1	T	C	9: 7,155,099 (GRCm38)	E895G	probably damaging	Het
Ehhadh	A	C	16: 21,762,692 (GRCm38)	S517A	probably benign	Het
Ephb2	C	A	4: 136,693,787 (GRCm38)	Q417H	possibly damaging	Het
Ephb4	A	G	5: 137,363,312 (GRCm38)	T526A	probably damaging	Het
Fam222b	G	A	11: 78,153,768 (GRCm38)	V52I	probably damaging	Het
Galm	G	T	17: 80,144,988 (GRCm38)	W118C	probably damaging	Het
Galm	G	A	17: 80,144,987 (GRCm38)	W118*	probably null	Het
Gcfc2	A	G	6: 81,944,386 (GRCm38)	N458D	probably damaging	Het
Gm11595	G	A	11: 99,772,555 (GRCm38)	R100C	unknown	Het
Gykl1	A	C	18: 52,694,651 (GRCm38)	R310S	possibly damaging	Het
Gzmn	A	C	14: 56,167,946 (GRCm38)	V27G	probably damaging	Het
H2-T23	T	A	17: 36,032,216 (GRCm38)	M90L	probably benign	Het
Hnrnpu	T	C	1: 178,337,312 (GRCm38)	E87G	unknown	Het
Hps3	G	A	3: 20,012,701 (GRCm38)	S567L	possibly damaging	Het
Igfn1	A	T	1: 135,964,938 (GRCm38)	V2148E	probably damaging	Het
Ighv1-75	T	C	12: 115,833,952 (GRCm38)	R117G	probably damaging	Het
Itgae	C	T	11: 73,145,638 (GRCm38)	A1134V	probably benign	Het
Kmt2b	C	T	7: 30,581,673 (GRCm38)	A1294T	possibly damaging	Het
Leng8	C	T	7: 4,145,473 (GRCm38)	T748I	probably damaging	Het
Lrig3	G	C	10: 126,006,690 (GRCm38)	D495H	probably damaging	Het
Mctp1	G	A	13: 76,712,079 (GRCm38)		probably null	Het
Mfsd3	T	A	15: 76,702,171 (GRCm38)	L168*	probably null	Het
Nlrp4d	C	A	7: 10,362,782 (GRCm38)	G921*	probably null	Het
Nsun4	G	A	4: 116,034,138 (GRCm38)	T348I	probably damaging	Het
Nucb1	T	C	7: 45,498,418 (GRCm38)	T246A	probably damaging	Het
Nynrin	A	C	14: 55,863,806 (GRCm38)	S311R	probably damaging	Het
Olfr1019	T	G	2: 85,841,014 (GRCm38)	Y259S	probably damaging	Het
Olfr1281	T	A	2: 111,328,396 (GRCm38)		probably null	Het
Ovch2	C	A	7: 107,792,134 (GRCm38)	R303L	probably benign	Het
Pcsk9	A	G	4: 106,447,174 (GRCm38)	S490P	probably damaging	Het
Pi4ka	A	G	16: 17,323,030 (GRCm38)	F859L	probably benign	Het
Pkd1l3	A	G	8: 109,640,792 (GRCm38)	D1207G	probably damaging	Het
Pnpla7	G	A	2: 25,021,952 (GRCm38)	R710Q	possibly damaging	Het
Prex2	T	G	1: 11,200,032 (GRCm38)	S1314A	probably damaging	Het
Rnf216	A	G	5: 143,093,002 (GRCm38)	L64P	probably damaging	Het
Sh3bp5	C	A	14: 31,377,495 (GRCm38)	R265L	probably benign	Het
Slc6a20b	T	G	9: 123,603,834 (GRCm38)	S374R	possibly damaging	Het
Slc8a1	G	T	17: 81,649,224 (GRCm38)	N128K	probably damaging	Het
Slc9a3r1	T	A	11: 115,163,761 (GRCm38)	I79N	probably damaging	Het
Slfn5	A	T	11: 82,956,385 (GRCm38)	D32V	probably damaging	Het
Snrnp35	T	C	5: 124,490,490 (GRCm38)	I122T	possibly damaging	Het
Snx24	C	T	18: 53,340,211 (GRCm38)	Q76*	probably null	Het
Sspo	T	A	6: 48,454,850 (GRCm38)	H692Q	probably damaging	Het
Stxbp3	T	C	3: 108,793,798 (GRCm38)	D585G	probably damaging	Het
Svep1	T	C	4: 58,072,677 (GRCm38)	N2211D	possibly damaging	Het
Tnfrsf18	G	A	4: 156,028,424 (GRCm38)		probably null	Het
Tnik	T	G	3: 28,541,972 (GRCm38)	D171E	probably damaging	Het
Ttc23l	T	A	15: 10,533,659 (GRCm38)	H266L	probably damaging	Het
Ttn	G	T	2: 76,894,770 (GRCm38)	S2037*	probably null	Het
Tuba3a	G	A	6: 125,281,310 (GRCm38)	T239I	probably damaging	Het
Usp25	T	A	16: 77,093,706 (GRCm38)	D767E	probably benign	Het
Whrn	G	T	4: 63,431,843 (GRCm38)	H546N	probably benign	Het
Xirp2	C	T	2: 67,511,162 (GRCm38)	T1249I	probably damaging	Het
Zbtb1	T	A	12: 76,386,240 (GRCm38)	D333E	probably damaging	Het
Zfp689	T	G	7: 127,448,815 (GRCm38)	E15A	possibly damaging	Het
Zhx3	A	G	2: 160,779,868 (GRCm38)	M793T	probably benign	Het

Other mutations in Abca8b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00862:Abca8b	APN	11	109,953,548 (GRCm38)	missense	possibly damaging	0.66
IGL00952:Abca8b	APN	11	109,969,060 (GRCm38)	critical splice donor site	probably null
IGL01141:Abca8b	APN	11	109,937,730 (GRCm38)	missense	probably damaging	1.00
IGL01523:Abca8b	APN	11	109,976,494 (GRCm38)	missense	probably damaging	1.00
IGL01633:Abca8b	APN	11	109,936,754 (GRCm38)	missense	probably damaging	0.99
IGL01862:Abca8b	APN	11	109,947,171 (GRCm38)	nonsense	probably null
IGL01963:Abca8b	APN	11	109,971,763 (GRCm38)	missense	probably damaging	0.99
IGL02169:Abca8b	APN	11	109,952,582 (GRCm38)	missense	probably damaging	0.98
IGL02536:Abca8b	APN	11	109,981,748 (GRCm38)	missense	probably benign	0.02
IGL02658:Abca8b	APN	11	109,952,560 (GRCm38)	missense	probably benign
IGL02828:Abca8b	APN	11	109,980,894 (GRCm38)	missense	probably damaging	0.99
IGL03118:Abca8b	APN	11	109,947,181 (GRCm38)	missense	possibly damaging	0.66
IGL03302:Abca8b	APN	11	109,967,750 (GRCm38)	missense	possibly damaging	0.80
IGL03325:Abca8b	APN	11	109,953,596 (GRCm38)	missense	possibly damaging	0.94
R0057:Abca8b	UTSW	11	109,941,559 (GRCm38)	missense	possibly damaging	0.91
R0131:Abca8b	UTSW	11	109,942,289 (GRCm38)	missense	possibly damaging	0.46
R0226:Abca8b	UTSW	11	109,957,018 (GRCm38)	splice site	probably null
R0426:Abca8b	UTSW	11	109,955,027 (GRCm38)	splice site	probably benign
R0432:Abca8b	UTSW	11	109,980,015 (GRCm38)	missense	possibly damaging	0.94
R0512:Abca8b	UTSW	11	109,950,650 (GRCm38)	missense	probably benign	0.32
R0589:Abca8b	UTSW	11	109,942,268 (GRCm38)	missense	probably damaging	0.96
R0690:Abca8b	UTSW	11	109,969,808 (GRCm38)	splice site	probably benign
R1263:Abca8b	UTSW	11	109,941,607 (GRCm38)	missense	possibly damaging	0.66
R1371:Abca8b	UTSW	11	109,953,553 (GRCm38)	missense	probably damaging	0.99
R1497:Abca8b	UTSW	11	109,973,821 (GRCm38)	splice site	probably benign
R1502:Abca8b	UTSW	11	109,974,645 (GRCm38)	missense	probably damaging	1.00
R1517:Abca8b	UTSW	11	109,971,814 (GRCm38)	missense	possibly damaging	0.66
R1543:Abca8b	UTSW	11	109,974,674 (GRCm38)	missense	probably damaging	0.98
R1618:Abca8b	UTSW	11	109,949,888 (GRCm38)	splice site	probably benign
R1625:Abca8b	UTSW	11	109,967,121 (GRCm38)	missense	probably benign	0.11
R1753:Abca8b	UTSW	11	109,973,716 (GRCm38)	missense	probably benign	0.00
R1819:Abca8b	UTSW	11	109,981,056 (GRCm38)	critical splice acceptor site	probably null
R1822:Abca8b	UTSW	11	109,957,075 (GRCm38)	missense	possibly damaging	0.92
R1829:Abca8b	UTSW	11	109,942,341 (GRCm38)	missense	probably damaging	0.97
R1873:Abca8b	UTSW	11	109,979,955 (GRCm38)	missense	probably benign	0.01
R1899:Abca8b	UTSW	11	109,937,918 (GRCm38)	missense	possibly damaging	0.92
R1908:Abca8b	UTSW	11	109,957,098 (GRCm38)	missense	possibly damaging	0.92
R1962:Abca8b	UTSW	11	109,979,898 (GRCm38)	missense	probably benign	0.00
R1984:Abca8b	UTSW	11	109,977,841 (GRCm38)	missense	probably damaging	1.00
R2035:Abca8b	UTSW	11	109,957,106 (GRCm38)	missense	possibly damaging	0.94
R2092:Abca8b	UTSW	11	109,966,708 (GRCm38)	missense	possibly damaging	0.63
R2100:Abca8b	UTSW	11	109,937,782 (GRCm38)	missense	probably damaging	1.00
R2267:Abca8b	UTSW	11	109,955,148 (GRCm38)	missense	probably benign	0.03
R2871:Abca8b	UTSW	11	109,955,176 (GRCm38)	missense	possibly damaging	0.83
R2871:Abca8b	UTSW	11	109,955,176 (GRCm38)	missense	possibly damaging	0.83
R2872:Abca8b	UTSW	11	109,955,176 (GRCm38)	missense	possibly damaging	0.83
R2872:Abca8b	UTSW	11	109,955,176 (GRCm38)	missense	possibly damaging	0.83
R2873:Abca8b	UTSW	11	109,955,176 (GRCm38)	missense	possibly damaging	0.83
R3711:Abca8b	UTSW	11	109,946,255 (GRCm38)	missense	possibly damaging	0.46
R3937:Abca8b	UTSW	11	109,974,567 (GRCm38)	missense	probably benign	0.01
R4052:Abca8b	UTSW	11	109,981,725 (GRCm38)	nonsense	probably null
R4060:Abca8b	UTSW	11	109,957,201 (GRCm38)	missense	probably benign	0.04
R4207:Abca8b	UTSW	11	109,981,725 (GRCm38)	nonsense	probably null
R4208:Abca8b	UTSW	11	109,981,725 (GRCm38)	nonsense	probably null
R4354:Abca8b	UTSW	11	109,971,692 (GRCm38)	missense	probably benign	0.27
R4399:Abca8b	UTSW	11	109,936,385 (GRCm38)	missense	possibly damaging	0.66
R4456:Abca8b	UTSW	11	109,942,245 (GRCm38)	missense	probably benign	0.27
R4509:Abca8b	UTSW	11	109,966,755 (GRCm38)	missense	probably damaging	1.00
R4672:Abca8b	UTSW	11	109,936,448 (GRCm38)	missense	possibly damaging	0.81
R4868:Abca8b	UTSW	11	109,974,512 (GRCm38)	missense	probably benign	0.05
R5002:Abca8b	UTSW	11	109,961,797 (GRCm38)	missense	probably damaging	0.96
R5007:Abca8b	UTSW	11	109,936,764 (GRCm38)	missense	probably damaging	1.00
R5014:Abca8b	UTSW	11	109,950,131 (GRCm38)	missense	probably damaging	0.98
R5023:Abca8b	UTSW	11	109,974,988 (GRCm38)	critical splice donor site	probably null
R5091:Abca8b	UTSW	11	109,936,384 (GRCm38)	missense	possibly damaging	0.92
R5098:Abca8b	UTSW	11	109,957,118 (GRCm38)	missense	probably benign	0.05
R5117:Abca8b	UTSW	11	109,966,803 (GRCm38)	missense	probably damaging	1.00
R5234:Abca8b	UTSW	11	109,976,594 (GRCm38)	missense	possibly damaging	0.90
R5302:Abca8b	UTSW	11	109,977,813 (GRCm38)	missense	probably damaging	1.00
R5487:Abca8b	UTSW	11	109,953,514 (GRCm38)	missense	probably damaging	0.99
R5512:Abca8b	UTSW	11	109,977,813 (GRCm38)	missense	probably damaging	1.00
R5564:Abca8b	UTSW	11	109,934,581 (GRCm38)	missense	probably benign	0.08
R5610:Abca8b	UTSW	11	109,977,813 (GRCm38)	missense	probably damaging	1.00
R5677:Abca8b	UTSW	11	109,940,861 (GRCm38)	missense	probably damaging	1.00
R5723:Abca8b	UTSW	11	109,953,619 (GRCm38)	missense	possibly damaging	0.90
R5827:Abca8b	UTSW	11	109,977,813 (GRCm38)	missense	probably damaging	1.00
R5829:Abca8b	UTSW	11	109,977,813 (GRCm38)	missense	probably damaging	1.00
R5848:Abca8b	UTSW	11	109,977,813 (GRCm38)	missense	probably damaging	1.00
R5849:Abca8b	UTSW	11	109,977,813 (GRCm38)	missense	probably damaging	1.00
R5850:Abca8b	UTSW	11	109,977,813 (GRCm38)	missense	probably damaging	1.00
R5854:Abca8b	UTSW	11	109,977,813 (GRCm38)	missense	probably damaging	1.00
R5982:Abca8b	UTSW	11	109,953,597 (GRCm38)	missense	possibly damaging	0.80
R5994:Abca8b	UTSW	11	109,949,766 (GRCm38)	splice site	probably null
R6035:Abca8b	UTSW	11	109,971,860 (GRCm38)	splice site	probably null
R6035:Abca8b	UTSW	11	109,971,860 (GRCm38)	splice site	probably null
R6050:Abca8b	UTSW	11	109,977,813 (GRCm38)	missense	probably damaging	1.00
R6145:Abca8b	UTSW	11	109,973,808 (GRCm38)	missense	probably benign	0.03
R6223:Abca8b	UTSW	11	109,977,846 (GRCm38)	missense	probably benign	0.00
R6349:Abca8b	UTSW	11	109,934,718 (GRCm38)	splice site	probably null
R7002:Abca8b	UTSW	11	109,941,564 (GRCm38)	missense	probably damaging	1.00
R7050:Abca8b	UTSW	11	109,973,718 (GRCm38)	missense	possibly damaging	0.90
R7107:Abca8b	UTSW	11	109,976,473 (GRCm38)	missense	probably damaging	0.98
R7158:Abca8b	UTSW	11	109,934,589 (GRCm38)	missense	probably damaging	1.00
R7170:Abca8b	UTSW	11	109,945,828 (GRCm38)	missense	probably benign	0.09
R7197:Abca8b	UTSW	11	109,945,822 (GRCm38)	nonsense	probably null
R7220:Abca8b	UTSW	11	109,981,717 (GRCm38)	missense	probably damaging	1.00
R7512:Abca8b	UTSW	11	109,938,449 (GRCm38)	missense	probably benign	0.01
R7590:Abca8b	UTSW	11	109,938,515 (GRCm38)	missense	probably damaging	0.97
R7658:Abca8b	UTSW	11	109,935,717 (GRCm38)	missense	probably benign	0.00
R7739:Abca8b	UTSW	11	109,974,591 (GRCm38)	missense	probably benign	0.05
R7797:Abca8b	UTSW	11	109,971,683 (GRCm38)	critical splice donor site	probably null
R7934:Abca8b	UTSW	11	109,975,039 (GRCm38)	missense	possibly damaging	0.75
R8074:Abca8b	UTSW	11	109,938,494 (GRCm38)	missense	probably benign
R8302:Abca8b	UTSW	11	109,962,580 (GRCm38)	critical splice donor site	probably null
R8341:Abca8b	UTSW	11	109,955,050 (GRCm38)	missense	probably damaging	1.00
R8486:Abca8b	UTSW	11	109,967,111 (GRCm38)	missense	possibly damaging	0.83
R8748:Abca8b	UTSW	11	109,945,771 (GRCm38)	missense	probably damaging	1.00
R8924:Abca8b	UTSW	11	109,947,177 (GRCm38)	missense	probably benign	0.00
R9002:Abca8b	UTSW	11	109,952,630 (GRCm38)	missense	probably benign	0.02
R9032:Abca8b	UTSW	11	109,957,247 (GRCm38)	missense	probably benign	0.04
R9099:Abca8b	UTSW	11	109,980,882 (GRCm38)	missense	probably damaging	1.00
R9124:Abca8b	UTSW	11	109,937,767 (GRCm38)	missense	probably damaging	0.97
R9178:Abca8b	UTSW	11	109,950,111 (GRCm38)	missense	probably benign	0.00
R9188:Abca8b	UTSW	11	109,981,735 (GRCm38)	nonsense	probably null
R9277:Abca8b	UTSW	11	109,976,521 (GRCm38)	missense	probably damaging	0.99
R9340:Abca8b	UTSW	11	109,950,113 (GRCm38)	missense	probably benign	0.43
R9371:Abca8b	UTSW	11	109,967,672 (GRCm38)	missense	probably damaging	1.00
R9382:Abca8b	UTSW	11	109,979,885 (GRCm38)	missense	probably benign
R9450:Abca8b	UTSW	11	109,969,104 (GRCm38)	missense	probably damaging	0.98
R9462:Abca8b	UTSW	11	109,953,607 (GRCm38)	missense
R9712:Abca8b	UTSW	11	109,942,337 (GRCm38)	missense	probably benign	0.30
Z1088:Abca8b	UTSW	11	109,976,482 (GRCm38)	missense	probably benign	0.09
Z1176:Abca8b	UTSW	11	109,974,644 (GRCm38)	missense	possibly damaging	0.87
Z1176:Abca8b	UTSW	11	109,961,908 (GRCm38)	missense	possibly damaging	0.52

Predicted Primers

PCR Primer
(F):5'- TTGTTTTGTACCATACAGCAGC -3'
(R):5'- TCAAGGTAGGTGGTTCCCTG -3'

Sequencing Primer
(F):5'- GTACCATACAGCAGCTTTAGTAAC -3'
(R):5'- TAGGTGGTTCCCTGGGAGACC -3'

Posted On

2016-07-22