Incidental Mutation 'R5309:Adgrg3'
ID 404721
Institutional Source Beutler Lab
Gene Symbol Adgrg3
Ensembl Gene ENSMUSG00000060470
Gene Name adhesion G protein-coupled receptor G3
Synonyms Pb99, A030001G24Rik, Gpr97
MMRRC Submission 042892-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R5309 (G1)
Quality Score 225
Status Validated
Chromosome 8
Chromosomal Location 95744320-95771878 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) G to A at 95766492 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Valine to Isoleucine at position 388 (V388I)
Ref Sequence ENSEMBL: ENSMUSP00000051079 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000051259]
AlphaFold Q8R0T6
Predicted Effect probably benign
Transcript: ENSMUST00000051259
AA Change: V388I

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)
SMART Domains Protein: ENSMUSP00000051079
Gene: ENSMUSG00000060470
AA Change: V388I

DomainStartEndE-ValueType
signal peptide 1 18 N/A INTRINSIC
GPS 209 256 3.45e-11 SMART
Pfam:7tm_2 260 509 5.1e-33 PFAM
low complexity region 520 531 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000211974
Predicted Effect noncoding transcript
Transcript: ENSMUST00000211994
Predicted Effect probably benign
Transcript: ENSMUST00000212570
Meta Mutation Damage Score 0.0898 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.5%
  • 10x: 97.0%
  • 20x: 94.3%
Validation Efficiency 99% (68/69)
MGI Phenotype PHENOTYPE: Homozygous null mice display normal B and T cell development. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 61 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
A630073D07Rik T C 6: 132,603,540 (GRCm39) Q72R unknown Het
Abca15 T C 7: 119,944,592 (GRCm39) V409A probably damaging Het
Abcg3 A C 5: 105,084,465 (GRCm39) C577G possibly damaging Het
Adamtsl5 A T 10: 80,180,982 (GRCm39) probably benign Het
Ank2 T C 3: 126,753,417 (GRCm39) Q288R probably damaging Het
Cdc45 C T 16: 18,614,647 (GRCm39) R205H probably damaging Het
Cdh3 A G 8: 107,265,652 (GRCm39) T232A probably damaging Het
Cfap210 T C 2: 69,617,602 (GRCm39) T60A possibly damaging Het
Cntnap5c A T 17: 58,666,249 (GRCm39) E1093V probably benign Het
Cplx3 A T 9: 57,518,360 (GRCm39) L343Q probably damaging Het
Cwh43 A G 5: 73,574,110 (GRCm39) H258R probably benign Het
Cyp2j6 T A 4: 96,423,793 (GRCm39) I192F probably damaging Het
Dnaaf5 T A 5: 139,138,617 (GRCm39) V266E probably damaging Het
Egfr G A 11: 16,861,703 (GRCm39) G1161S probably benign Het
Ehmt1 A G 2: 24,774,207 (GRCm39) V201A probably damaging Het
Exoc7 C A 11: 116,195,853 (GRCm39) E28* probably null Het
Fam118a C T 15: 84,934,956 (GRCm39) T195M probably damaging Het
Fancg A G 4: 43,003,019 (GRCm39) F613L probably benign Het
Fbxo10 A T 4: 45,042,036 (GRCm39) I731N possibly damaging Het
Fchsd1 C T 18: 38,092,926 (GRCm39) probably benign Het
Gfm2 G A 13: 97,299,659 (GRCm39) A406T probably damaging Het
Gnal G A 18: 67,346,178 (GRCm39) R219K possibly damaging Het
Helz2 T A 2: 180,876,639 (GRCm39) E1285V probably benign Het
Ighv1-74 A G 12: 115,766,501 (GRCm39) S39P probably damaging Het
Ipo11 T C 13: 106,970,481 (GRCm39) probably benign Het
Klc1 A G 12: 111,762,055 (GRCm39) K575R possibly damaging Het
Larp1 T C 11: 57,941,634 (GRCm39) V689A possibly damaging Het
Mki67 A T 7: 135,302,559 (GRCm39) V825E probably damaging Het
Mmp9 T A 2: 164,792,715 (GRCm39) probably benign Het
Myog A G 1: 134,218,064 (GRCm39) K91E probably damaging Het
Nfil3 A T 13: 53,121,656 (GRCm39) V416E probably damaging Het
Nup160 G T 2: 90,563,176 (GRCm39) E1314* probably null Het
Or4g17 T C 2: 111,210,179 (GRCm39) V278A possibly damaging Het
Or4k35 T G 2: 111,100,655 (GRCm39) D19A probably benign Het
Or6c66b A C 10: 129,377,134 (GRCm39) M243L probably benign Het
Or6c75 T A 10: 129,337,383 (GRCm39) V210E probably damaging Het
Osbpl8 T A 10: 111,106,418 (GRCm39) V275E probably benign Het
Osbpl9 A G 4: 108,923,352 (GRCm39) S520P probably damaging Het
Ppp4r4 T A 12: 103,573,147 (GRCm39) probably null Het
Proz T C 8: 13,111,049 (GRCm39) L7P probably damaging Het
Ptpn13 G A 5: 103,688,919 (GRCm39) S904N probably damaging Het
Rap1gds1 A G 3: 138,664,389 (GRCm39) L322P probably damaging Het
Rnf5 A G 17: 34,820,562 (GRCm39) F175S probably benign Het
Sema4a G A 3: 88,344,343 (GRCm39) S636F probably damaging Het
Sfrp2 A G 3: 83,676,708 (GRCm39) D193G probably damaging Het
Shoc2 T C 19: 53,976,164 (GRCm39) V18A probably benign Het
Skint8 C A 4: 111,807,390 (GRCm39) L359M probably damaging Het
Slc10a6 A T 5: 103,756,958 (GRCm39) C269S probably damaging Het
Slc34a2 A G 5: 53,226,830 (GRCm39) E651G probably damaging Het
Snx13 C T 12: 35,194,324 (GRCm39) Q956* probably null Het
Spg21 A G 9: 65,376,084 (GRCm39) I31V probably benign Het
Srpk2 T C 5: 23,730,716 (GRCm39) K268E probably damaging Het
Supt16 T C 14: 52,400,155 (GRCm39) E996G probably damaging Het
Syf2 A G 4: 134,663,380 (GRCm39) D184G probably benign Het
Tmem45a2 T C 16: 56,859,370 (GRCm39) D287G possibly damaging Het
Utrn A T 10: 12,603,513 (GRCm39) D627E probably damaging Het
Vmn1r170 T A 7: 23,305,880 (GRCm39) I94N probably damaging Het
Vmn2r103 A T 17: 20,013,296 (GRCm39) N139I probably benign Het
Vmn2r15 T A 5: 109,440,956 (GRCm39) I301F probably damaging Het
Vps35l T C 7: 118,412,799 (GRCm39) I629T probably damaging Het
Zfp949 A C 9: 88,449,236 (GRCm39) T14P possibly damaging Het
Other mutations in Adgrg3
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01096:Adgrg3 APN 8 95,766,221 (GRCm39) missense possibly damaging 0.90
IGL01724:Adgrg3 APN 8 95,766,053 (GRCm39) missense probably benign 0.04
IGL02111:Adgrg3 APN 8 95,761,627 (GRCm39) missense probably damaging 0.96
IGL02142:Adgrg3 APN 8 95,766,483 (GRCm39) missense probably damaging 1.00
IGL02577:Adgrg3 APN 8 95,766,555 (GRCm39) missense probably damaging 0.99
IGL02940:Adgrg3 APN 8 95,760,084 (GRCm39) missense possibly damaging 0.48
IGL03395:Adgrg3 APN 8 95,761,701 (GRCm39) missense probably damaging 1.00
R0111:Adgrg3 UTSW 8 95,761,738 (GRCm39) splice site probably benign
R0288:Adgrg3 UTSW 8 95,766,568 (GRCm39) missense possibly damaging 0.92
R0403:Adgrg3 UTSW 8 95,763,550 (GRCm39) missense probably benign 0.07
R1553:Adgrg3 UTSW 8 95,766,896 (GRCm39) missense possibly damaging 0.90
R1667:Adgrg3 UTSW 8 95,760,001 (GRCm39) nonsense probably null
R1686:Adgrg3 UTSW 8 95,759,997 (GRCm39) missense probably benign 0.02
R1872:Adgrg3 UTSW 8 95,760,070 (GRCm39) missense possibly damaging 0.87
R1882:Adgrg3 UTSW 8 95,766,943 (GRCm39) missense probably benign 0.03
R1924:Adgrg3 UTSW 8 95,762,562 (GRCm39) missense probably benign
R1998:Adgrg3 UTSW 8 95,763,296 (GRCm39) missense probably damaging 1.00
R2090:Adgrg3 UTSW 8 95,766,558 (GRCm39) missense possibly damaging 0.54
R2696:Adgrg3 UTSW 8 95,747,702 (GRCm39) missense probably benign 0.01
R3846:Adgrg3 UTSW 8 95,767,049 (GRCm39) missense probably benign 0.07
R4013:Adgrg3 UTSW 8 95,761,727 (GRCm39) splice site probably benign
R4405:Adgrg3 UTSW 8 95,763,536 (GRCm39) missense probably benign 0.15
R4622:Adgrg3 UTSW 8 95,767,153 (GRCm39) missense probably damaging 1.00
R4878:Adgrg3 UTSW 8 95,761,714 (GRCm39) missense possibly damaging 0.86
R5101:Adgrg3 UTSW 8 95,763,563 (GRCm39) missense probably benign 0.00
R5312:Adgrg3 UTSW 8 95,766,492 (GRCm39) missense probably benign 0.00
R5353:Adgrg3 UTSW 8 95,762,556 (GRCm39) missense probably damaging 0.99
R5820:Adgrg3 UTSW 8 95,766,221 (GRCm39) missense possibly damaging 0.90
R6240:Adgrg3 UTSW 8 95,766,544 (GRCm39) missense probably benign 0.23
R6272:Adgrg3 UTSW 8 95,762,889 (GRCm39) missense noncoding transcript
R7110:Adgrg3 UTSW 8 95,761,591 (GRCm39) missense possibly damaging 0.62
R7645:Adgrg3 UTSW 8 95,761,392 (GRCm39) intron probably benign
R8178:Adgrg3 UTSW 8 95,761,675 (GRCm39) missense probably damaging 0.98
R8397:Adgrg3 UTSW 8 95,767,141 (GRCm39) missense probably benign 0.01
R8730:Adgrg3 UTSW 8 95,766,556 (GRCm39) missense probably benign 0.09
R8951:Adgrg3 UTSW 8 95,761,362 (GRCm39) intron probably benign
R9100:Adgrg3 UTSW 8 95,762,891 (GRCm39) intron probably benign
R9523:Adgrg3 UTSW 8 95,766,186 (GRCm39) missense probably benign 0.06
R9583:Adgrg3 UTSW 8 95,760,071 (GRCm39) missense probably benign 0.00
R9589:Adgrg3 UTSW 8 95,760,093 (GRCm39) missense possibly damaging 0.87
X0017:Adgrg3 UTSW 8 95,744,398 (GRCm39) start codon destroyed probably null 0.53
Predicted Primers PCR Primer
(F):5'- CTATTTCCTGAAGCTGAGCCTG -3'
(R):5'- AACTGGATCCGGCTATCCTC -3'

Sequencing Primer
(F):5'- CTGCTGGCCTGGGGTAG -3'
(R):5'- CCAGCCTTGACATTGCTGG -3'
Posted On 2016-07-22