Mutagenetix > Incidental Mutation

Incidental Mutation 'R5324:Mroh9'

405001

Institutional Source

Beutler Lab

Gene Symbol

Mroh9

Ensembl Gene

ENSMUSG00000071890

Gene Name

maestro heat-like repeat family member 9

Synonyms

4921528O07Rik, Armc11

MMRRC Submission

042907-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5324 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

163024302-163085670 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to G at 163060760 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Arginine at position 249 (G249R)

Ref Sequence

ENSEMBL: ENSMUSP00000094365 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000096608]

AlphaFold

G5E8L9

Predicted Effect

probably damaging
Transcript: ENSMUST00000096608
AA Change: G249R

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000094365
Gene: ENSMUSG00000071890
AA Change: G249R

Domain	Start	End	E-Value	Type
SCOP:d1gw5b_	231	716	2e-8	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000195132

Meta Mutation Damage Score

0.1745

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.6%
20x: 96.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 35 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9130019O22Rik	G	T	7: 127,384,907		probably benign	Het
Akap10	C	T	11: 61,916,189	A72T	probably damaging	Het
Ap5b1	G	A	19: 5,569,835	E428K	possibly damaging	Het
Bmp2	C	T	2: 133,561,359	R277*	probably null	Het
Cbl	A	T	9: 44,154,254	S659T	probably damaging	Het
Col14a1	A	T	15: 55,338,445	H43L	unknown	Het
Corin	A	G	5: 72,435,257	C133R	probably damaging	Het
Cyp1a1	A	G	9: 57,702,369	N401S	probably benign	Het
Dip2a	T	C	10: 76,296,393	D508G	probably damaging	Het
Dnah2	T	A	11: 69,457,993	H2556L	probably benign	Het
Dock8	T	C	19: 25,163,094	F1333L	probably benign	Het
Epg5	A	G	18: 77,962,445	K717E	possibly damaging	Het
Fmn2	G	A	1: 174,608,880		probably benign	Het
Gm4787	G	C	12: 81,377,830	T518S	probably benign	Het
Hbp1	T	C	12: 31,928,618	N510S	probably damaging	Het
Lrguk	T	C	6: 34,073,797	S397P	possibly damaging	Het
Mmrn1	A	T	6: 60,976,586	D617V	probably damaging	Het
N6amt1	A	G	16: 87,354,353	D34G	probably damaging	Het
Nktr	A	T	9: 121,727,346	D30V	probably damaging	Het
Olfr1151	A	T	2: 87,857,696	I174F	probably damaging	Het
Olfr598	T	A	7: 103,329,050	M188K	probably damaging	Het
Olfr805	T	A	10: 129,722,945	I200L	probably benign	Het
Pabpc1	A	G	15: 36,600,625	F314L	probably damaging	Het
Papln	G	A	12: 83,774,571	V226M	probably damaging	Het
Parp12	A	T	6: 39,102,612	D321E	probably damaging	Het
Plch2	T	A	4: 154,984,534	T1107S	probably benign	Het
Psma2	T	C	13: 14,625,217	L182P	probably damaging	Het
Rcl1	A	G	19: 29,128,001	Y196C	probably benign	Het
Rdh16	G	A	10: 127,801,267	V24M	probably damaging	Het
Rpe65	A	G	3: 159,604,404	T105A	possibly damaging	Het
Serpini1	T	C	3: 75,640,294	I371T	probably damaging	Het
Tet2	T	C	3: 133,485,913	N920S	probably benign	Het
Tmem71	C	T	15: 66,555,214	S44N	probably benign	Het
Tmprss11f	T	A	5: 86,556,978	D27V	possibly damaging	Het
Zxdc	T	A	6: 90,373,800	I411N	probably damaging	Het

Other mutations in Mroh9

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00593:Mroh9	APN	1	163045781	missense	possibly damaging	0.89
IGL00705:Mroh9	APN	1	163079503	missense	probably damaging	1.00
IGL00788:Mroh9	APN	1	163024658	missense	probably benign	0.06
IGL00795:Mroh9	APN	1	163060622	missense	probably damaging	1.00
IGL00815:Mroh9	APN	1	163039131	missense	probably damaging	1.00
IGL01025:Mroh9	APN	1	163047866	missense	possibly damaging	0.67
IGL01303:Mroh9	APN	1	163080575	missense	probably benign	0.00
IGL01526:Mroh9	APN	1	163055603	missense	probably damaging	0.99
IGL01680:Mroh9	APN	1	163047982	splice site	probably null
IGL01823:Mroh9	APN	1	163055609	missense	probably benign	0.39
IGL02024:Mroh9	APN	1	163062502	missense	possibly damaging	0.65
IGL02213:Mroh9	APN	1	163058079	missense	probably damaging	1.00
IGL02455:Mroh9	APN	1	163075580	missense	probably benign	0.03
IGL02546:Mroh9	APN	1	163080576	missense	probably benign	0.04
IGL03059:Mroh9	APN	1	163024636	missense	possibly damaging	0.95
IGL03061:Mroh9	APN	1	163026502	missense	probably damaging	1.00
IGL03071:Mroh9	APN	1	163039197	missense	probably damaging	1.00
R0048:Mroh9	UTSW	1	163062487	missense	probably damaging	0.97
R0048:Mroh9	UTSW	1	163062487	missense	probably damaging	0.97
R0441:Mroh9	UTSW	1	163060762	missense	probably damaging	1.00
R0506:Mroh9	UTSW	1	163060636	missense	possibly damaging	0.90
R0629:Mroh9	UTSW	1	163060636	missense	possibly damaging	0.90
R0751:Mroh9	UTSW	1	163066124	missense	possibly damaging	0.84
R1301:Mroh9	UTSW	1	163043983	critical splice donor site	probably null
R1481:Mroh9	UTSW	1	163026509	missense	probably damaging	1.00
R1618:Mroh9	UTSW	1	163024541	missense	probably benign	0.00
R1647:Mroh9	UTSW	1	163046056	missense	probably damaging	1.00
R1648:Mroh9	UTSW	1	163046056	missense	probably damaging	1.00
R1668:Mroh9	UTSW	1	163024592	missense	possibly damaging	0.52
R1795:Mroh9	UTSW	1	163056778	missense	probably damaging	0.97
R1796:Mroh9	UTSW	1	163045710	missense	probably damaging	1.00
R1857:Mroh9	UTSW	1	163039145	missense	probably damaging	0.98
R1869:Mroh9	UTSW	1	163026513	missense	probably damaging	0.97
R1923:Mroh9	UTSW	1	163076291	missense	probably damaging	1.00
R2325:Mroh9	UTSW	1	163026530	splice site	probably null
R2511:Mroh9	UTSW	1	163038945	missense	probably benign	0.13
R2912:Mroh9	UTSW	1	163044003	missense	probably damaging	1.00
R2913:Mroh9	UTSW	1	163044003	missense	probably damaging	1.00
R2919:Mroh9	UTSW	1	163056772	missense	probably damaging	1.00
R2973:Mroh9	UTSW	1	163056769	missense	probably damaging	1.00
R3912:Mroh9	UTSW	1	163066069	missense	probably damaging	0.97
R4034:Mroh9	UTSW	1	163080553	critical splice donor site	probably null
R4551:Mroh9	UTSW	1	163044093	missense	probably damaging	0.98
R4656:Mroh9	UTSW	1	163066024	missense	probably damaging	1.00
R4662:Mroh9	UTSW	1	163055593	missense	probably damaging	0.97
R4743:Mroh9	UTSW	1	163024492	missense	probably benign	0.05
R4890:Mroh9	UTSW	1	163026524	missense	probably damaging	1.00
R5128:Mroh9	UTSW	1	163060760	missense	probably damaging	1.00
R5129:Mroh9	UTSW	1	163060760	missense	probably damaging	1.00
R5147:Mroh9	UTSW	1	163060760	missense	probably damaging	1.00
R5157:Mroh9	UTSW	1	163044121	missense	probably damaging	0.96
R5325:Mroh9	UTSW	1	163060760	missense	probably damaging	1.00
R5340:Mroh9	UTSW	1	163080587	start gained	probably benign
R6005:Mroh9	UTSW	1	163075677	missense	probably damaging	0.99
R6182:Mroh9	UTSW	1	163066043	nonsense	probably null
R6414:Mroh9	UTSW	1	163074702	missense	probably damaging	1.00
R6477:Mroh9	UTSW	1	163076304	missense	probably damaging	1.00
R6540:Mroh9	UTSW	1	163038972	missense	possibly damaging	0.87
R6541:Mroh9	UTSW	1	163058038	missense	possibly damaging	0.78
R6643:Mroh9	UTSW	1	163075561	missense	probably damaging	1.00
R6811:Mroh9	UTSW	1	163046041	missense	possibly damaging	0.86
R6830:Mroh9	UTSW	1	163076366	missense	probably benign
R7026:Mroh9	UTSW	1	163060682	missense	probably benign	0.00
R7052:Mroh9	UTSW	1	163038956	missense	possibly damaging	0.92
R7068:Mroh9	UTSW	1	163039181	missense	probably damaging	1.00
R7350:Mroh9	UTSW	1	163076289	critical splice donor site	probably null
R7545:Mroh9	UTSW	1	163074708	missense	possibly damaging	0.56
R7615:Mroh9	UTSW	1	163046032	missense	probably benign	0.40
R7743:Mroh9	UTSW	1	163024553	missense	probably benign
R7808:Mroh9	UTSW	1	163039109	missense	probably damaging	1.00
R8024:Mroh9	UTSW	1	163039233	missense	probably benign	0.02
R8062:Mroh9	UTSW	1	163038975	missense	probably damaging	1.00
R8145:Mroh9	UTSW	1	163062527	missense	probably benign	0.00
R8426:Mroh9	UTSW	1	163024725	missense	probably damaging	0.98
R8458:Mroh9	UTSW	1	163055681	missense	probably damaging	1.00
R8555:Mroh9	UTSW	1	163072026	splice site	probably null
R8960:Mroh9	UTSW	1	163055627	missense	probably benign	0.25
R9040:Mroh9	UTSW	1	163062500	missense	probably benign	0.06
R9125:Mroh9	UTSW	1	163047843	missense	probably benign	0.19
R9154:Mroh9	UTSW	1	163062461	missense
R9596:Mroh9	UTSW	1	163066007	missense	probably damaging	0.98
R9612:Mroh9	UTSW	1	163038929	missense	probably damaging	1.00
RF003:Mroh9	UTSW	1	163058061	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGACTTTTGAGAGCACGCTC -3'
(R):5'- TTGCCTAATAGTACCTGGAACTTCC -3'

Sequencing Primer
(F):5'- GAGCACGCTCTGTTCTTCTTAG -3'
(R):5'- GGAACTTCCATCATCATTGCAC -3'

Posted On

2016-07-22