Mutagenetix > Incidental Mutation

Incidental Mutation 'R5294:Vmn2r89'

405327

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r89

Ensembl Gene

ENSMUSG00000070448

Gene Name

vomeronasal 2, receptor 89

Synonyms

V2r10, V2r11

MMRRC Submission

042877-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.208) question?

Stock #

R5294 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

51451962-51461293 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 51455113 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Lysine at position 124 (N124K)

Ref Sequence

ENSEMBL: ENSMUSP00000124256 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000159611] [ENSMUST00000159734] [ENSMUST00000161670] [ENSMUST00000162998]

AlphaFold

O35199

Predicted Effect

probably benign
Transcript: ENSMUST00000159611
AA Change: N124K

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000124065
Gene: ENSMUSG00000070448
AA Change: N124K

Domain	Start	End	E-Value	Type
signal peptide	1	26	N/A	INTRINSIC
Pfam:ANF_receptor	76	449	4.8e-30	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000159734
AA Change: N124K

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)

SMART Domains

Protein: ENSMUSP00000124256
Gene: ENSMUSG00000070448
AA Change: N124K

Domain	Start	End	E-Value	Type
signal peptide	1	26	N/A	INTRINSIC
Pfam:ANF_receptor	76	420	1.1e-29	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000161670

SMART Domains

Protein: ENSMUSP00000124261
Gene: ENSMUSG00000070448

Domain	Start	End	E-Value	Type
signal peptide	1	26	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000162998

SMART Domains

Protein: ENSMUSP00000125409
Gene: ENSMUSG00000068399

Domain	Start	End	E-Value	Type
Pfam:Takusan	35	115	2.2e-25	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.5%
20x: 96.0%

Validation Efficiency

97% (70/72)

Allele List at MGI

Other mutations in this stock

Total: 70 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930579C12Rik	T	C	9: 89,152,003 (GRCm38)		noncoding transcript	Het
Acaca	A	G	11: 84,391,519 (GRCm38)	E2154G	probably benign	Het
Acacb	T	C	5: 114,241,952 (GRCm38)	F2056L	probably damaging	Het
Aff1	A	G	5: 103,811,157 (GRCm38)		probably benign	Het
Amn1	T	A	6: 149,185,124 (GRCm38)		probably benign	Het
Arid1a	C	A	4: 133,691,055 (GRCm38)		probably benign	Het
Aste1	T	A	9: 105,402,705 (GRCm38)		probably null	Het
Asxl3	T	A	18: 22,516,439 (GRCm38)	V495D	possibly damaging	Het
Atp1a3	T	A	7: 24,988,048 (GRCm38)	H688L	probably damaging	Het
B3gnt8	T	C	7: 25,628,766 (GRCm38)	L207P	probably damaging	Het
Baz2b	T	C	2: 59,978,602 (GRCm38)	H101R	probably benign	Het
Bicc1	G	A	10: 70,947,900 (GRCm38)	T387M	possibly damaging	Het
Champ1	A	C	8: 13,878,981 (GRCm38)	K380Q	probably damaging	Het
Cnst	A	G	1: 179,610,440 (GRCm38)	E523G	probably benign	Het
Cops6	G	C	5: 138,161,116 (GRCm38)		probably benign	Het
Cp	G	C	3: 19,966,316 (GRCm38)	V158L	probably benign	Het
Cyfip1	T	A	7: 55,873,483 (GRCm38)	M52K	possibly damaging	Het
Dars	A	T	1: 128,364,302 (GRCm38)	F480I	probably benign	Het
Diaph1	T	C	18: 37,897,580 (GRCm38)	M274V	unknown	Het
Diaph1	C	A	18: 37,897,550 (GRCm38)	E284*	probably null	Het
Dock8	G	A	19: 25,061,153 (GRCm38)	V68M	probably benign	Het
Elavl4	A	G	4: 110,211,430 (GRCm38)	F247L	possibly damaging	Het
Emc10	C	T	7: 44,496,439 (GRCm38)		probably benign	Het
Fbxw16	T	C	9: 109,436,644 (GRCm38)	D369G	probably benign	Het
Fgr	A	T	4: 132,997,500 (GRCm38)	D304V	probably benign	Het
Filip1l	G	A	16: 57,570,036 (GRCm38)	S91N	possibly damaging	Het
Gm884	A	G	11: 103,616,231 (GRCm38)		probably benign	Het
Haus8	A	G	8: 71,255,710 (GRCm38)	S103P	unknown	Het
Hscb	A	G	5: 110,834,792 (GRCm38)	L143P	probably damaging	Het
Hsd11b2	A	T	8: 105,523,297 (GRCm38)	M347L	probably benign	Het
Jrk	C	A	15: 74,707,336 (GRCm38)	E33D	possibly damaging	Het
Kbtbd8	T	A	6: 95,121,832 (GRCm38)	Y123*	probably null	Het
Mis18bp1	A	C	12: 65,157,043 (GRCm38)	M59R	probably damaging	Het
Mrps27	T	C	13: 99,409,873 (GRCm38)	V260A	probably damaging	Het
Ncapg2	G	T	12: 116,427,794 (GRCm38)	V488L	possibly damaging	Het
Nepn	A	T	10: 52,400,800 (GRCm38)	N211Y	probably benign	Het
Ntrk3	A	T	7: 78,517,506 (GRCm38)		probably null	Het
Olfr248	A	T	1: 174,391,225 (GRCm38)	Y52F	probably benign	Het
Olfr692	C	T	7: 105,368,413 (GRCm38)	T20I	probably benign	Het
Olfr748	A	G	14: 50,710,779 (GRCm38)	I150V	probably benign	Het
Olfr748	A	G	14: 50,710,443 (GRCm38)	T38A	possibly damaging	Het
Otud4	A	T	8: 79,672,892 (GRCm38)	Q744L	possibly damaging	Het
P2ry14	A	T	3: 59,115,568 (GRCm38)	I166N	possibly damaging	Het
Pak2	T	A	16: 32,021,830 (GRCm38)	N478Y	probably damaging	Het
Papss2	A	G	19: 32,639,000 (GRCm38)	D202G	probably benign	Het
Pcdh7	C	A	5: 57,728,111 (GRCm38)		probably null	Het
Peg3	C	A	7: 6,717,849 (GRCm38)	S19I	possibly damaging	Het
Prim2	G	T	1: 33,668,893 (GRCm38)	T40K	probably benign	Het
Ranbp2	T	C	10: 58,478,668 (GRCm38)	F1737L	probably benign	Het
Rex2	A	C	4: 147,057,985 (GRCm38)	N310T	probably benign	Het
Rnf123	AT	ATT	9: 108,064,003 (GRCm38)		probably null	Het
Rnf39	C	T	17: 36,947,200 (GRCm38)	A86V	probably damaging	Het
Ror1	A	T	4: 100,425,938 (GRCm38)	N400I	probably benign	Het
Slc38a8	C	T	8: 119,494,289 (GRCm38)	G177D	probably damaging	Het
Slc43a3	T	C	2: 84,956,310 (GRCm38)	V445A	probably benign	Het
Sptbn2	A	G	19: 4,718,908 (GRCm38)	N23S	possibly damaging	Het
Taf5l	A	G	8: 124,008,218 (GRCm38)	F74L	probably benign	Het
Trappc11	G	C	8: 47,530,731 (GRCm38)	A42G	possibly damaging	Het
Trim30d	T	C	7: 104,472,488 (GRCm38)	K350R	probably damaging	Het
Trnt1	T	C	6: 106,773,414 (GRCm38)	F93S	probably damaging	Het
Ube2c	T	C	2: 164,777,190 (GRCm38)	V161A	probably benign	Het
Usp24	A	G	4: 106,362,357 (GRCm38)	E555G	possibly damaging	Het
Vmn2r55	T	G	7: 12,651,864 (GRCm38)	S730R	probably damaging	Het
Vmn2r98	T	A	17: 19,069,754 (GRCm38)	C517*	probably null	Het
Vps13a	A	T	19: 16,641,667 (GRCm38)	I2845N	probably damaging	Het
Vps51	T	G	19: 6,071,033 (GRCm38)	E283D	probably benign	Het
Xpo5	T	C	17: 46,236,922 (GRCm38)	V896A	probably benign	Het
Zfp2	T	C	11: 50,901,241 (GRCm38)		probably benign	Het
Zgrf1	G	A	3: 127,600,980 (GRCm38)	M1328I	probably benign	Het
Zswim5	A	G	4: 116,979,577 (GRCm38)	D686G	possibly damaging	Het

Other mutations in Vmn2r89

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00433:Vmn2r89	APN	14	51,454,965 (GRCm38)	missense	probably damaging	1.00
IGL00990:Vmn2r89	APN	14	51,457,493 (GRCm38)	missense	probably benign
IGL00990:Vmn2r89	APN	14	51,455,971 (GRCm38)	missense	probably benign	0.14
IGL01991:Vmn2r89	APN	14	51,452,219 (GRCm38)	missense	probably benign	0.00
IGL03073:Vmn2r89	APN	14	51,456,071 (GRCm38)	missense	possibly damaging	0.95
IGL03085:Vmn2r89	APN	14	51,452,158 (GRCm38)	missense	probably damaging	0.99
IGL03278:Vmn2r89	APN	14	51,455,100 (GRCm38)	missense	probably damaging	0.99
R0115:Vmn2r89	UTSW	14	51,456,120 (GRCm38)	missense	probably damaging	1.00
R0127:Vmn2r89	UTSW	14	51,455,703 (GRCm38)	missense	probably damaging	0.98
R0391:Vmn2r89	UTSW	14	51,455,978 (GRCm38)	missense	probably damaging	0.99
R0481:Vmn2r89	UTSW	14	51,456,120 (GRCm38)	missense	probably damaging	1.00
R0538:Vmn2r89	UTSW	14	51,457,591 (GRCm38)	splice site	probably null
R1210:Vmn2r89	UTSW	14	51,454,970 (GRCm38)	missense	probably benign	0.01
R1332:Vmn2r89	UTSW	14	51,455,102 (GRCm38)	missense	probably benign	0.00
R1660:Vmn2r89	UTSW	14	51,456,236 (GRCm38)	missense	possibly damaging	0.48
R1959:Vmn2r89	UTSW	14	51,457,440 (GRCm38)	missense	probably benign	0.22
R2876:Vmn2r89	UTSW	14	51,455,084 (GRCm38)	missense	possibly damaging	0.47
R3410:Vmn2r89	UTSW	14	51,456,171 (GRCm38)	missense	probably damaging	0.98
R4026:Vmn2r89	UTSW	14	51,452,043 (GRCm38)	start codon destroyed	probably null	1.00
R4398:Vmn2r89	UTSW	14	51,452,094 (GRCm38)	missense	probably damaging	1.00
R4700:Vmn2r89	UTSW	14	51,457,485 (GRCm38)	missense	probably damaging	1.00
R4714:Vmn2r89	UTSW	14	51,452,231 (GRCm38)	missense	probably damaging	0.97
R5162:Vmn2r89	UTSW	14	51,456,163 (GRCm38)	missense	possibly damaging	0.88
R5811:Vmn2r89	UTSW	14	51,456,108 (GRCm38)	missense	probably benign	0.12
R6087:Vmn2r89	UTSW	14	51,457,576 (GRCm38)	splice site	probably null
R6229:Vmn2r89	UTSW	14	51,455,721 (GRCm38)	missense	probably benign	0.05
R6246:Vmn2r89	UTSW	14	51,456,046 (GRCm38)	missense	probably damaging	1.00
R6572:Vmn2r89	UTSW	14	51,455,993 (GRCm38)	missense	probably damaging	1.00
R7351:Vmn2r89	UTSW	14	51,456,282 (GRCm38)	missense	probably benign	0.30
R7683:Vmn2r89	UTSW	14	51,455,194 (GRCm38)	missense	probably benign
R7974:Vmn2r89	UTSW	14	51,456,002 (GRCm38)	missense	probably damaging	1.00
R8047:Vmn2r89	UTSW	14	51,455,092 (GRCm38)	missense	probably benign	0.05
R8093:Vmn2r89	UTSW	14	51,456,242 (GRCm38)	missense	probably benign	0.00
R8348:Vmn2r89	UTSW	14	51,455,091 (GRCm38)	missense	possibly damaging	0.90
R8723:Vmn2r89	UTSW	14	51,456,453 (GRCm38)	missense	probably benign
R8737:Vmn2r89	UTSW	14	51,456,265 (GRCm38)	missense	probably damaging	1.00
R8859:Vmn2r89	UTSW	14	51,455,713 (GRCm38)	missense	probably benign
R9183:Vmn2r89	UTSW	14	51,455,044 (GRCm38)	missense	probably benign	0.01
R9197:Vmn2r89	UTSW	14	51,456,139 (GRCm38)	missense	possibly damaging	0.70
R9377:Vmn2r89	UTSW	14	51,455,144 (GRCm38)	missense	probably benign	0.02
R9395:Vmn2r89	UTSW	14	51,456,326 (GRCm38)	missense	probably damaging	1.00
R9452:Vmn2r89	UTSW	14	51,455,831 (GRCm38)	missense	probably damaging	0.99
R9457:Vmn2r89	UTSW	14	51,456,012 (GRCm38)	missense	probably damaging	0.99
R9678:Vmn2r89	UTSW	14	51,456,054 (GRCm38)	missense	probably benign	0.09
X0019:Vmn2r89	UTSW	14	51,456,415 (GRCm38)	missense	possibly damaging	0.95

Predicted Primers

PCR Primer
(F):5'- TCTAGTTCAAGGATGGCATTGAG -3'
(R):5'- GCACACTCATAAATGCATTCTGTCC -3'

Sequencing Primer
(F):5'- TTCAAGGATGGCATTGAGATAAAAC -3'
(R):5'- GCATTCTGTCCAAATTTTCTTGTAGG -3'

Posted On

2016-07-22