Mutagenetix > Incidental Mutation

Incidental Mutation 'R5297:Or10x4'

405451

Institutional Source

Beutler Lab

Gene Symbol

Or10x4

Ensembl Gene

ENSMUSG00000059503

Gene Name

olfactory receptor family 10 subfamily X member 4

Synonyms

MOR267-7, GA_x6K02T2MFC0-1145-1312, GA_x6K02T2P20D-20771141-20770212, Olfr415, Olfr248

MMRRC Submission

042880-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.075) question?

Stock #

R5297 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

174391046-174392055 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 174391200 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Valine at position 44 (M44V)

Ref Sequence

ENSEMBL: ENSMUSP00000074799 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000075329]

AlphaFold

E9Q1L6

Predicted Effect

probably benign
Transcript: ENSMUST00000075329
AA Change: M44V

PolyPhen 2 Score 0.206 (Sensitivity: 0.92; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000074799
Gene: ENSMUSG00000059503
AA Change: M44V

Domain	Start	End	E-Value	Type
Pfam:7tm_4	29	304	1.2e-40	PFAM
Pfam:7tm_1	39	303	7.5e-22	PFAM

Coding Region Coverage

1x: 99.4%
3x: 98.7%
10x: 97.4%
20x: 95.6%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acbd6	G	T	1: 155,587,458 (GRCm38)	A135S	probably benign	Het
Agbl4	A	G	4: 111,566,698 (GRCm38)	K307R	possibly damaging	Het
Akna	T	C	4: 63,381,846 (GRCm38)	E653G	possibly damaging	Het
Arhgap26	T	C	18: 39,121,888 (GRCm38)	Y273H	probably damaging	Het
Atg14	G	A	14: 47,568,199 (GRCm38)	R70C	probably damaging	Het
Atp1a1	C	T	3: 101,591,127 (GRCm38)	V250M	possibly damaging	Het
Cacna1c	T	G	6: 118,742,361 (GRCm38)	D215A	probably damaging	Het
Cadps	T	A	14: 12,822,345 (GRCm38)	N132Y	probably damaging	Het
Casp6	T	C	3: 129,910,555 (GRCm38)	F97L	possibly damaging	Het
Ckap2l	T	C	2: 129,285,370 (GRCm38)	N296S	possibly damaging	Het
Col6a4	T	C	9: 106,074,867 (GRCm38)	K611E	probably benign	Het
Copa	A	G	1: 172,113,108 (GRCm38)	H696R	probably damaging	Het
Cyp2a4	T	A	7: 26,312,204 (GRCm38)	N283K	probably benign	Het
Dcc	C	T	18: 71,378,738 (GRCm38)	V869I	probably benign	Het
Efemp1	G	T	11: 28,867,868 (GRCm38)	G116C	probably damaging	Het
F830045P16Rik	T	C	2: 129,460,553 (GRCm38)	E373G	probably benign	Het
Fbxo9	T	C	9: 78,086,279 (GRCm38)	T318A	probably benign	Het
Gipr	A	G	7: 19,157,544 (GRCm38)	W403R	probably damaging	Het
Gm4787	G	C	12: 81,377,830 (GRCm38)	T518S	probably benign	Het
Golgb1	G	A	16: 36,875,616 (GRCm38)		probably benign	Het
Hdac1-ps	A	G	17: 78,492,758 (GRCm38)	S393G	probably benign	Het
Herc3	T	C	6: 58,856,641 (GRCm38)	L171P	probably damaging	Het
Il5ra	A	G	6: 106,738,134 (GRCm38)	I221T	probably benign	Het
Itgb2	A	G	10: 77,564,667 (GRCm38)	I705V	probably damaging	Het
Map4k4	G	T	1: 39,962,217 (GRCm38)	V55F	probably damaging	Het
Mast1	A	G	8: 84,913,318 (GRCm38)		probably null	Het
Mitf	G	T	6: 97,994,430 (GRCm38)	G186V	probably benign	Het
Mtpn	C	T	6: 35,512,290 (GRCm38)	D100N	probably benign	Het
Nrcam	T	C	12: 44,544,784 (GRCm38)	F204L	probably damaging	Het
Or10g1	A	G	14: 52,410,218 (GRCm38)	L218P	probably damaging	Het
Or1e16	AGCGGTCGTAGGC	AGC	11: 73,395,654 (GRCm38)		probably null	Het
Or1e22	A	T	11: 73,486,389 (GRCm38)	V145E	probably damaging	Het
Or2a12	T	C	6: 42,927,437 (GRCm38)	S69P	probably benign	Het
Or52n4b	A	T	7: 108,545,404 (GRCm38)	N291I	probably damaging	Het
Or5as1	T	C	2: 87,150,449 (GRCm38)	I71V	probably benign	Het
Pclo	A	T	5: 14,676,249 (GRCm38)		probably benign	Het
Pik3ca	T	A	3: 32,450,053 (GRCm38)	Y631N	probably damaging	Het
Pramel19	T	A	4: 101,941,151 (GRCm38)	D106E	possibly damaging	Het
Ptk2b	T	C	14: 66,172,517 (GRCm38)	D462G	probably benign	Het
Rnf19a	A	G	15: 36,247,778 (GRCm38)	S427P	probably damaging	Het
Scn3a	T	A	2: 65,469,034 (GRCm38)	Y1376F	possibly damaging	Het
Shoc1	A	G	4: 59,047,543 (GRCm38)	W1359R	probably benign	Het
Spem1	A	G	11: 69,820,927 (GRCm38)	Y304H	probably damaging	Het
Stk38l	C	A	6: 146,775,655 (GRCm38)	Y450*	probably null	Het
Stx19	A	G	16: 62,821,974 (GRCm38)	E51G	probably damaging	Het
Ttc41	C	G	10: 86,776,579 (GRCm38)	Q1239E	probably benign	Het
Tuba3a	C	T	6: 125,281,340 (GRCm38)	R229H	probably damaging	Het
Utp18	A	T	11: 93,876,089 (GRCm38)	V264D	probably damaging	Het
V1rd19	T	C	7: 24,003,289 (GRCm38)	V60A	probably damaging	Het
Virma	T	G	4: 11,494,819 (GRCm38)	V40G	probably damaging	Het
Vmn1r160	C	T	7: 22,871,290 (GRCm38)	Q23*	probably null	Het
Vmn2r13	T	C	5: 109,191,939 (GRCm38)	I57V	probably benign	Het
Vmn2r26	T	A	6: 124,061,873 (GRCm38)	F802L	probably damaging	Het
Vmn2r61	A	G	7: 42,260,222 (GRCm38)	D57G	probably benign	Het
Vps13c	A	G	9: 67,878,131 (GRCm38)	N260S	probably damaging	Het
Xirp1	C	A	9: 120,019,602 (GRCm38)	A72S	probably damaging	Het
Zfp212	T	C	6: 47,929,077 (GRCm38)	V190A	probably benign	Het
Zfp703	C	T	8: 26,979,205 (GRCm38)	P299L	probably damaging	Het

Other mutations in Or10x4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01649:Or10x4	APN	1	174,391,408 (GRCm38)	missense	probably damaging	1.00
R0375:Or10x4	UTSW	1	174,391,209 (GRCm38)	missense	probably damaging	1.00
R1720:Or10x4	UTSW	1	174,391,920 (GRCm38)	missense	probably benign	0.00
R1906:Or10x4	UTSW	1	174,391,164 (GRCm38)	missense	probably damaging	1.00
R1996:Or10x4	UTSW	1	174,391,417 (GRCm38)	missense	probably damaging	1.00
R2009:Or10x4	UTSW	1	174,391,429 (GRCm38)	missense	possibly damaging	0.94
R4745:Or10x4	UTSW	1	174,391,876 (GRCm38)	missense	probably damaging	1.00
R5294:Or10x4	UTSW	1	174,391,225 (GRCm38)	missense	probably benign
R5665:Or10x4	UTSW	1	174,391,375 (GRCm38)	missense	probably damaging	1.00
R5784:Or10x4	UTSW	1	174,391,399 (GRCm38)	missense	probably damaging	1.00
R5857:Or10x4	UTSW	1	174,391,108 (GRCm38)	missense	possibly damaging	0.59
R6943:Or10x4	UTSW	1	174,391,841 (GRCm38)	nonsense	probably null
R6975:Or10x4	UTSW	1	174,391,677 (GRCm38)	missense	probably benign	0.10
R7114:Or10x4	UTSW	1	174,391,239 (GRCm38)	missense	probably damaging	0.96
R7614:Or10x4	UTSW	1	174,391,654 (GRCm38)	missense	probably damaging	0.99
R8036:Or10x4	UTSW	1	174,391,816 (GRCm38)	missense	probably damaging	1.00
R8674:Or10x4	UTSW	1	174,391,692 (GRCm38)	missense	probably damaging	1.00
R8777:Or10x4	UTSW	1	174,391,282 (GRCm38)	missense	probably damaging	1.00
R8777-TAIL:Or10x4	UTSW	1	174,391,282 (GRCm38)	missense	probably damaging	1.00
R9139:Or10x4	UTSW	1	174,391,083 (GRCm38)	missense	probably damaging	1.00
R9178:Or10x4	UTSW	1	174,391,663 (GRCm38)	missense	probably benign	0.36
R9256:Or10x4	UTSW	1	174,391,543 (GRCm38)	missense	probably benign	0.11
R9449:Or10x4	UTSW	1	174,391,176 (GRCm38)	missense	probably benign
R9668:Or10x4	UTSW	1	174,391,332 (GRCm38)	missense	probably benign	0.04

Predicted Primers

PCR Primer
(F):5'- TACAGGGTTGAGTTTTGCCAAAC -3'
(R):5'- TTTGTTCCTCCAAGGCCCAG -3'

Sequencing Primer
(F):5'- AAGAAGCATTGAAGGTTTATTTGTTC -3'
(R):5'- GGCCCAGGAAGAAACACATCTG -3'

Posted On

2016-07-22