Incidental Mutation 'R5299:Igtp'
ID405576
Institutional Source Beutler Lab
Gene Symbol Igtp
Ensembl Gene ENSMUSG00000078853
Gene Nameinterferon gamma induced GTPase
SynonymsIrgm3
MMRRC Submission 042882-MU
Accession Numbers
Is this an essential gene? Non essential (E-score: 0.000) question?
Stock #R5299 (G1)
Quality Score225
Status Not validated
Chromosome11
Chromosomal Location58199556-58207591 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) T to C at 58207133 bp
ZygosityHeterozygous
Amino Acid Change Tryptophan to Arginine at position 377 (W377R)
Ref Sequence ENSEMBL: ENSMUSP00000047356 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000035266] [ENSMUST00000058704] [ENSMUST00000094169] [ENSMUST00000168280]
Predicted Effect possibly damaging
Transcript: ENSMUST00000035266
AA Change: W377R

PolyPhen 2 Score 0.788 (Sensitivity: 0.85; Specificity: 0.93)
SMART Domains Protein: ENSMUSP00000047356
Gene: ENSMUSG00000078853
AA Change: W377R

DomainStartEndE-ValueType
Pfam:IIGP 50 412 4.6e-161 PFAM
Pfam:MMR_HSR1 86 200 1.1e-6 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000058704
SMART Domains Protein: ENSMUSP00000056001
Gene: ENSMUSG00000069874

DomainStartEndE-ValueType
Pfam:IIGP 30 387 8.1e-165 PFAM
Pfam:MMR_HSR1 66 179 9.3e-7 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000094169
Predicted Effect probably benign
Transcript: ENSMUST00000168280
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.5%
  • 10x: 96.7%
  • 20x: 93.5%
Validation Efficiency
MGI Phenotype PHENOTYPE: Mice homozygous for disruptions in this gene display an essentially normal phenotype. They do have a severely compromised response to Toxoplasma gondii infections however [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 39 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abca13 A G 11: 9,431,861 I3838V probably damaging Het
Arid1a A T 4: 133,687,226 D1231E unknown Het
Atxn1 A T 13: 45,557,254 I734K probably benign Het
Axin1 A G 17: 26,173,734 S330G probably damaging Het
Bend3 G A 10: 43,493,690 probably null Het
Chodl C T 16: 78,941,408 T88I probably damaging Het
Dnah2 C T 11: 69,458,920 R2399Q probably benign Het
Dst A G 1: 34,135,092 I179M probably damaging Het
Ehmt2 C T 17: 34,899,091 R40* probably null Het
Exoc2 A T 13: 30,871,918 probably null Het
Grk2 T C 19: 4,292,771 E45G probably damaging Het
Ift81 T C 5: 122,607,056 Y144C probably damaging Het
Ighv1-15 T C 12: 114,657,378 D109G probably damaging Het
Lgr6 C T 1: 134,994,010 A199T probably damaging Het
Map2k6 A G 11: 110,492,963 D145G probably benign Het
Mcph1 T C 8: 18,652,580 probably benign Het
Mdfi A G 17: 47,820,834 V95A possibly damaging Het
Mical3 A G 6: 120,959,512 L1351P possibly damaging Het
Nbas C T 12: 13,441,925 Q1506* probably null Het
Nelfb A G 2: 25,210,745 V128A probably benign Het
Otog A G 7: 46,288,851 T1995A probably benign Het
Ppp1r7 A T 1: 93,352,626 I139L probably benign Het
Ppp2r1b A G 9: 50,857,040 D19G probably benign Het
Proca1 T C 11: 78,205,252 S150P probably damaging Het
Rhof A G 5: 123,120,548 V100A probably damaging Het
Rsbn1 G C 3: 103,914,490 G14R probably benign Het
Serinc1 A G 10: 57,523,051 I252T probably damaging Het
Skint4 A T 4: 112,136,006 I309F possibly damaging Het
Slc12a3 A G 8: 94,351,789 Y815C probably damaging Het
Slc25a24 A G 3: 109,166,352 S424G probably benign Het
Spint2 G A 7: 29,263,726 A49V probably damaging Het
Traf3ip3 T A 1: 193,178,175 K480* probably null Het
Ube2g2 T C 10: 77,644,545 S162P possibly damaging Het
Ubxn8 C A 8: 33,641,919 V7L possibly damaging Het
Vmn1r200 T A 13: 22,395,775 C249* probably null Het
Vmn1r38 T A 6: 66,776,698 T145S probably benign Het
Wapl G A 14: 34,733,808 probably null Het
Wfdc6a T C 2: 164,580,391 N96S possibly damaging Het
Zfp503 A G 14: 21,985,439 S470P probably benign Het
Other mutations in Igtp
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01138:Igtp APN 11 58206144 missense possibly damaging 0.66
IGL03326:Igtp APN 11 58206228 missense probably benign 0.01
R0281:Igtp UTSW 11 58206054 missense probably damaging 0.97
R2762:Igtp UTSW 11 58206065 missense possibly damaging 0.91
R3426:Igtp UTSW 11 58206593 missense probably damaging 0.99
R3427:Igtp UTSW 11 58206593 missense probably damaging 0.99
R3428:Igtp UTSW 11 58206593 missense probably damaging 0.99
R4484:Igtp UTSW 11 58206998 missense possibly damaging 0.91
R4588:Igtp UTSW 11 58206682 missense probably damaging 1.00
R5652:Igtp UTSW 11 58206629 missense probably benign 0.07
R5662:Igtp UTSW 11 58206279 missense probably damaging 1.00
R5893:Igtp UTSW 11 58206648 missense probably damaging 1.00
R7657:Igtp UTSW 11 58206828 missense probably benign 0.02
R7738:Igtp UTSW 11 58207080 missense probably benign 0.00
Predicted Primers PCR Primer
(F):5'- ACCAGAAGCTCTTTGGTGTGG -3'
(R):5'- CTTTCTAGTGACTAGCTAGGGC -3'

Sequencing Primer
(F):5'- GATGATGGGTCACTTCAGCAG -3'
(R):5'- TGAGGCAGCCTGAATTCCAC -3'
Posted On2016-07-22