Mutagenetix > Incidental Mutation

Incidental Mutation 'R5315:Vmn2r2'

405782

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r2

Ensembl Gene

ENSMUSG00000043897

Gene Name

vomeronasal 2, receptor 2

Synonyms

MMRRC Submission

042898-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.093) question?

Stock #

R5315 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

64022699-64049349 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 64024377 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Phenylalanine at position 735 (V735F)

Ref Sequence

ENSEMBL: ENSMUSP00000135110 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000077958] [ENSMUST00000177151]

AlphaFold

L7N2E9

Predicted Effect

probably benign
Transcript: ENSMUST00000077958
AA Change: V651F

PolyPhen 2 Score 0.242 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000077109
Gene: ENSMUSG00000043897
AA Change: V651F

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	1	416	4.5e-80	PFAM
Pfam:NCD3G	458	511	8.3e-18	PFAM
Pfam:7tm_3	542	779	1.3e-75	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000177151
AA Change: V735F

PolyPhen 2 Score 0.373 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000135110
Gene: ENSMUSG00000043897
AA Change: V735F

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	83	500	1.6e-84	PFAM
Pfam:NCD3G	542	595	2.6e-17	PFAM
Pfam:7tm_3	628	862	1.9e-47	PFAM

Coding Region Coverage

1x: 99.5%
3x: 99.0%
10x: 98.1%
20x: 97.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 34 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ace3	T	C	11: 105,885,747 (GRCm39)	V51A	probably benign	Het
Ass1	A	T	2: 31,382,341 (GRCm39)	I171F	probably benign	Het
AU041133	T	G	10: 81,987,506 (GRCm39)	Y386*	probably null	Het
Bex6	A	G	16: 32,005,311 (GRCm39)	N40D	probably benign	Het
Cc2d2a	A	G	5: 43,877,775 (GRCm39)	Y1044C	probably damaging	Het
Cflar	C	G	1: 58,792,961 (GRCm39)	D442E	probably benign	Het
Dag1	A	G	9: 108,086,316 (GRCm39)	V275A	probably damaging	Het
Dnah17	G	A	11: 118,018,109 (GRCm39)	R129W	possibly damaging	Het
Epha4	C	T	1: 77,365,109 (GRCm39)		probably null	Het
Eri2	T	C	7: 119,385,241 (GRCm39)	D420G	probably benign	Het
Gm42669	A	T	5: 107,656,103 (GRCm39)	I1301F	probably damaging	Het
Golga2	A	G	2: 32,193,773 (GRCm39)	E463G	probably damaging	Het
Grsf1	G	A	5: 88,821,634 (GRCm39)		probably benign	Het
Igkv6-15	A	C	6: 70,383,957 (GRCm39)	V7G	possibly damaging	Het
Katnal2	A	T	18: 77,099,705 (GRCm39)	V143D	probably benign	Het
Mepce	A	C	5: 137,780,955 (GRCm39)	I617S	probably damaging	Het
Mms19	C	T	19: 41,943,201 (GRCm39)	R320Q	possibly damaging	Het
Nt5c2	T	C	19: 46,880,682 (GRCm39)	Y353C	probably damaging	Het
Or5p76	A	G	7: 108,123,097 (GRCm39)	L20S	probably damaging	Het
Or8s5	G	A	15: 98,238,246 (GRCm39)	A208V	probably benign	Het
Pitpnm2	A	T	5: 124,259,996 (GRCm39)	D1111E	probably benign	Het
Plin5	C	A	17: 56,421,066 (GRCm39)	V200L	probably benign	Het
Rab3a	T	C	8: 71,208,569 (GRCm39)	F23L	probably damaging	Het
Rrp8	T	C	7: 105,383,207 (GRCm39)	K353R	probably benign	Het
S100a7l2	G	A	3: 90,997,637 (GRCm39)	T26M	possibly damaging	Het
Senp7	A	G	16: 56,000,889 (GRCm39)	D887G	probably benign	Het
Sftpb	G	T	6: 72,283,876 (GRCm39)	A158S	probably benign	Het
Sh2d2a	A	C	3: 87,754,976 (GRCm39)	T23P	probably damaging	Het
Siglecf	T	C	7: 43,004,532 (GRCm39)	L287P	probably benign	Het
Slc4a1	T	A	11: 102,249,080 (GRCm39)	I233F	possibly damaging	Het
Spef2	T	C	15: 9,596,777 (GRCm39)	Q1424R	probably damaging	Het
Tbc1d14	T	C	5: 36,664,932 (GRCm39)	D567G	probably damaging	Het
Tmem106b	T	G	6: 13,081,559 (GRCm39)	N155K	probably damaging	Het
Zfp850	T	C	7: 27,689,743 (GRCm39)	K155R	probably benign	Het

Other mutations in Vmn2r2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00326:Vmn2r2	APN	3	64,041,319 (GRCm39)	splice site	probably benign
IGL00980:Vmn2r2	APN	3	64,024,601 (GRCm39)	missense	probably benign	0.11
IGL01389:Vmn2r2	APN	3	64,024,430 (GRCm39)	missense	probably damaging	0.97
IGL01804:Vmn2r2	APN	3	64,041,677 (GRCm39)	missense	possibly damaging	0.91
IGL02750:Vmn2r2	APN	3	64,024,823 (GRCm39)	missense	probably damaging	1.00
IGL02829:Vmn2r2	APN	3	64,026,172 (GRCm39)	splice site	probably benign
IGL03036:Vmn2r2	APN	3	64,024,321 (GRCm39)	missense	probably benign	0.01
IGL03174:Vmn2r2	APN	3	64,024,544 (GRCm39)	nonsense	probably null
PIT4151001:Vmn2r2	UTSW	3	64,024,334 (GRCm39)	missense	possibly damaging	0.67
R0029:Vmn2r2	UTSW	3	64,024,365 (GRCm39)	missense	probably benign	0.00
R0310:Vmn2r2	UTSW	3	64,042,039 (GRCm39)	missense	probably damaging	1.00
R0357:Vmn2r2	UTSW	3	64,041,320 (GRCm39)	splice site	probably null
R0637:Vmn2r2	UTSW	3	64,033,999 (GRCm39)	missense	probably benign	0.00
R1626:Vmn2r2	UTSW	3	64,041,921 (GRCm39)	missense	possibly damaging	0.61
R1662:Vmn2r2	UTSW	3	64,024,551 (GRCm39)	missense	probably benign	0.00
R1710:Vmn2r2	UTSW	3	64,024,820 (GRCm39)	missense	probably benign	0.19
R1797:Vmn2r2	UTSW	3	64,042,128 (GRCm39)	missense	probably benign	0.00
R1862:Vmn2r2	UTSW	3	64,041,942 (GRCm39)	missense	possibly damaging	0.79
R1971:Vmn2r2	UTSW	3	64,034,121 (GRCm39)	missense	probably damaging	1.00
R2051:Vmn2r2	UTSW	3	64,024,766 (GRCm39)	missense	possibly damaging	0.77
R2099:Vmn2r2	UTSW	3	64,024,474 (GRCm39)	missense	probably damaging	1.00
R2275:Vmn2r2	UTSW	3	64,023,930 (GRCm39)	missense	probably benign	0.00
R3078:Vmn2r2	UTSW	3	64,042,053 (GRCm39)	missense	probably benign	0.22
R3418:Vmn2r2	UTSW	3	64,024,320 (GRCm39)	missense	probably benign	0.00
R3419:Vmn2r2	UTSW	3	64,024,320 (GRCm39)	missense	probably benign	0.00
R3959:Vmn2r2	UTSW	3	64,047,947 (GRCm39)	missense	probably benign
R4230:Vmn2r2	UTSW	3	64,041,912 (GRCm39)	missense	probably benign	0.00
R4258:Vmn2r2	UTSW	3	64,042,118 (GRCm39)	missense	probably damaging	1.00
R4810:Vmn2r2	UTSW	3	64,044,883 (GRCm39)	missense	probably damaging	1.00
R4822:Vmn2r2	UTSW	3	64,041,960 (GRCm39)	missense	probably damaging	0.99
R4919:Vmn2r2	UTSW	3	64,024,578 (GRCm39)	missense	possibly damaging	0.56
R4925:Vmn2r2	UTSW	3	64,044,892 (GRCm39)	start codon destroyed	probably null	0.03
R4954:Vmn2r2	UTSW	3	64,047,905 (GRCm39)	missense	probably benign	0.00
R5071:Vmn2r2	UTSW	3	64,024,321 (GRCm39)	missense	probably benign	0.01
R5450:Vmn2r2	UTSW	3	64,034,011 (GRCm39)	missense	probably benign
R5577:Vmn2r2	UTSW	3	64,024,416 (GRCm39)	missense	probably benign	0.00
R5595:Vmn2r2	UTSW	3	64,034,036 (GRCm39)	missense	possibly damaging	0.74
R5727:Vmn2r2	UTSW	3	64,024,608 (GRCm39)	missense	probably benign	0.41
R5810:Vmn2r2	UTSW	3	64,024,815 (GRCm39)	missense	probably damaging	1.00
R5919:Vmn2r2	UTSW	3	64,044,723 (GRCm39)	missense	probably benign	0.39
R6052:Vmn2r2	UTSW	3	64,024,782 (GRCm39)	missense	possibly damaging	0.56
R6084:Vmn2r2	UTSW	3	64,024,467 (GRCm39)	missense	probably benign	0.01
R6299:Vmn2r2	UTSW	3	64,024,074 (GRCm39)	nonsense	probably null
R6762:Vmn2r2	UTSW	3	64,041,870 (GRCm39)	missense	probably damaging	1.00
R6858:Vmn2r2	UTSW	3	64,044,915 (GRCm39)	missense	probably damaging	1.00
R6889:Vmn2r2	UTSW	3	64,024,688 (GRCm39)	missense	probably damaging	0.99
R6990:Vmn2r2	UTSW	3	64,024,608 (GRCm39)	missense	probably benign	0.02
R7195:Vmn2r2	UTSW	3	64,023,900 (GRCm39)	missense	probably benign	0.01
R7269:Vmn2r2	UTSW	3	64,033,998 (GRCm39)	missense	probably benign	0.32
R7699:Vmn2r2	UTSW	3	64,024,536 (GRCm39)	missense	possibly damaging	0.69
R7717:Vmn2r2	UTSW	3	64,042,019 (GRCm39)	missense	possibly damaging	0.95
R7798:Vmn2r2	UTSW	3	64,041,518 (GRCm39)	missense	possibly damaging	0.60
R7914:Vmn2r2	UTSW	3	64,041,526 (GRCm39)	missense	probably benign	0.20
R7974:Vmn2r2	UTSW	3	64,024,808 (GRCm39)	missense	probably damaging	0.99
R8394:Vmn2r2	UTSW	3	64,044,858 (GRCm39)	missense	probably damaging	1.00
R8478:Vmn2r2	UTSW	3	64,024,257 (GRCm39)	missense	possibly damaging	0.87
R8731:Vmn2r2	UTSW	3	64,024,404 (GRCm39)	missense	probably benign	0.16
R9035:Vmn2r2	UTSW	3	64,024,172 (GRCm39)	missense	probably damaging	1.00
R9182:Vmn2r2	UTSW	3	64,044,802 (GRCm39)	missense	probably damaging	1.00
R9225:Vmn2r2	UTSW	3	64,034,021 (GRCm39)	missense	probably benign	0.00
R9266:Vmn2r2	UTSW	3	64,024,057 (GRCm39)	missense	probably damaging	0.98
R9427:Vmn2r2	UTSW	3	64,041,945 (GRCm39)	missense	probably damaging	1.00
R9659:Vmn2r2	UTSW	3	64,041,942 (GRCm39)	missense	possibly damaging	0.79
R9666:Vmn2r2	UTSW	3	64,023,870 (GRCm39)	missense	probably benign	0.08
R9771:Vmn2r2	UTSW	3	64,042,079 (GRCm39)	missense	possibly damaging	0.88
R9788:Vmn2r2	UTSW	3	64,041,942 (GRCm39)	missense	possibly damaging	0.79
X0024:Vmn2r2	UTSW	3	64,044,707 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- TAGTTATCTGGCAGCTGGCG -3'
(R):5'- GCTGTTCATAGGCAAGCCATG -3'

Sequencing Primer
(F):5'- CTGGCGAGCCACAAAAGTTG -3'
(R):5'- AAGCCATGCAACTGGTCCTG -3'

Posted On

2016-07-22