Incidental Mutation 'R4806:Zfp341'
ID 406140
Institutional Source Beutler Lab
Gene Symbol Zfp341
Ensembl Gene ENSMUSG00000059842
Gene Name zinc finger protein 341
Synonyms
MMRRC Submission 042425-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R4806 (G1)
Quality Score 25
Status Validated
Chromosome 2
Chromosomal Location 154455217-154488741 bp(+) (GRCm39)
Type of Mutation utr 3 prime
DNA Base Change (assembly) G to A at 154487786 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change
Ref Sequence ENSEMBL: ENSMUSP00000117383 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000081926] [ENSMUST00000109702] [ENSMUST00000126421]
AlphaFold Q6PGC9
Predicted Effect unknown
Transcript: ENSMUST00000081926
AA Change: A757T
SMART Domains Protein: ENSMUSP00000080596
Gene: ENSMUSG00000059842
AA Change: A757T

DomainStartEndE-ValueType
ZnF_C2H2 53 73 4.16e1 SMART
low complexity region 96 110 N/A INTRINSIC
low complexity region 154 161 N/A INTRINSIC
low complexity region 177 200 N/A INTRINSIC
ZnF_C2H2 320 342 3.69e-4 SMART
ZnF_C2H2 348 370 1.04e-3 SMART
ZnF_C2H2 442 464 1.45e-2 SMART
ZnF_C2H2 470 494 2.17e-1 SMART
ZnF_C2H2 500 522 2.91e-2 SMART
ZnF_C2H2 537 561 1.23e0 SMART
ZnF_C2H2 563 585 3.58e-2 SMART
ZnF_C2H2 591 613 1.62e0 SMART
ZnF_C2H2 619 641 2.27e-4 SMART
ZnF_C2H2 647 674 7.29e0 SMART
ZnF_C2H2 680 702 5.14e-3 SMART
low complexity region 740 759 N/A INTRINSIC
Predicted Effect unknown
Transcript: ENSMUST00000109702
AA Change: A750T
SMART Domains Protein: ENSMUSP00000105324
Gene: ENSMUSG00000059842
AA Change: A750T

DomainStartEndE-ValueType
ZnF_C2H2 53 73 4.16e1 SMART
low complexity region 96 110 N/A INTRINSIC
low complexity region 154 161 N/A INTRINSIC
low complexity region 177 200 N/A INTRINSIC
ZnF_C2H2 313 335 3.69e-4 SMART
ZnF_C2H2 341 363 1.04e-3 SMART
ZnF_C2H2 435 457 1.45e-2 SMART
ZnF_C2H2 463 487 2.17e-1 SMART
ZnF_C2H2 493 515 2.91e-2 SMART
ZnF_C2H2 530 554 1.23e0 SMART
ZnF_C2H2 556 578 3.58e-2 SMART
ZnF_C2H2 584 606 1.62e0 SMART
ZnF_C2H2 612 634 2.27e-4 SMART
ZnF_C2H2 640 667 7.29e0 SMART
ZnF_C2H2 673 695 5.14e-3 SMART
low complexity region 733 752 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000126421
Meta Mutation Damage Score 0.0898 question?
Coding Region Coverage
  • 1x: 99.1%
  • 3x: 98.4%
  • 10x: 96.7%
  • 20x: 93.5%
Validation Efficiency 100% (66/66)
Allele List at MGI
Other mutations in this stock
Total: 61 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
2310022A10Rik A G 7: 27,265,070 (GRCm39) probably null Het
2410137M14Rik T A 17: 37,289,746 (GRCm39) H28L probably benign Het
4930562C15Rik T C 16: 4,667,536 (GRCm39) F309S unknown Het
Acd A G 8: 106,424,922 (GRCm39) V406A possibly damaging Het
Acin1 A T 14: 54,916,685 (GRCm39) probably benign Het
Agbl4 T C 4: 110,812,834 (GRCm39) V118A probably damaging Het
Arhgap5 A G 12: 52,565,486 (GRCm39) D819G probably damaging Het
BC049715 A T 6: 136,816,927 (GRCm39) I56F possibly damaging Het
C1rb A T 6: 124,551,908 (GRCm39) Q270L probably benign Het
Cadps2 C T 6: 23,688,859 (GRCm39) R121Q probably damaging Het
Cd84 A G 1: 171,679,688 (GRCm39) Y122C probably benign Het
Cklf C A 8: 104,984,067 (GRCm39) P77T probably damaging Het
Clrn2 T C 5: 45,611,346 (GRCm39) L65P probably damaging Het
Csde1 TCCTCGACCT TCCT 3: 102,963,685 (GRCm39) probably benign Het
Csf2rb2 T C 15: 78,169,490 (GRCm39) D446G probably benign Het
Csmd3 T C 15: 48,177,464 (GRCm39) E358G probably benign Het
Dmkn C T 7: 30,470,667 (GRCm39) T385I possibly damaging Het
Dnah10 A G 5: 124,896,408 (GRCm39) T3591A probably damaging Het
Dpp9 A G 17: 56,497,030 (GRCm39) L734P probably damaging Het
Edem2 A G 2: 155,570,913 (GRCm39) V39A possibly damaging Het
Glmp A C 3: 88,233,320 (GRCm39) probably benign Het
Gm20775 T C Y: 10,641,885 (GRCm39) noncoding transcript Het
Gpr179 T G 11: 97,240,610 (GRCm39) D271A possibly damaging Het
Gtf2ird1 A G 5: 134,412,750 (GRCm39) V587A probably damaging Het
Igfn1 T A 1: 135,895,095 (GRCm39) T1824S probably benign Het
Ighmbp2 A T 19: 3,311,589 (GRCm39) I942N probably damaging Het
Ints2 A G 11: 86,147,035 (GRCm39) L37P probably benign Het
Irgq T A 7: 24,233,470 (GRCm39) L437Q probably damaging Het
Kcnq4 A T 4: 120,570,291 (GRCm39) W351R probably damaging Het
Kif3b G A 2: 153,162,288 (GRCm39) A500T probably damaging Het
Lpp A G 16: 24,480,430 (GRCm39) D66G probably damaging Het
Mapkap1 G A 2: 34,487,434 (GRCm39) probably null Het
Mc4r T A 18: 66,992,559 (GRCm39) I185F probably damaging Het
Mdga1 T C 17: 30,061,128 (GRCm39) D621G probably benign Het
Med13 A G 11: 86,189,403 (GRCm39) S1169P probably benign Het
Myh11 A T 16: 14,018,947 (GRCm39) probably null Het
Naip1 G A 13: 100,562,129 (GRCm39) A1012V probably benign Het
Ntn4 C T 10: 93,480,362 (GRCm39) R29C probably damaging Het
Plb1 G A 5: 32,447,196 (GRCm39) G321D probably damaging Het
Plxnd1 A G 6: 115,937,816 (GRCm39) V1510A probably damaging Het
Polr1e T A 4: 45,024,482 (GRCm39) M131K probably benign Het
Prdm10 T A 9: 31,241,237 (GRCm39) *342R probably null Het
Prex2 T C 1: 11,138,244 (GRCm39) F108L probably damaging Het
Psmg2 T A 18: 67,781,992 (GRCm39) I186N probably benign Het
Ros1 T A 10: 51,972,271 (GRCm39) E1614D probably damaging Het
Sin3a G A 9: 56,994,026 (GRCm39) V44M probably damaging Het
Slco1a1 G A 6: 141,854,735 (GRCm39) L639F possibly damaging Het
Smr2 T G 5: 88,246,289 (GRCm39) L101* probably null Het
Spata31d1b C T 13: 59,863,535 (GRCm39) P228S probably benign Het
Stat5b G T 11: 100,681,623 (GRCm39) H544N probably benign Het
Syk A T 13: 52,786,963 (GRCm39) Y319F probably benign Het
Tln2 G T 9: 67,239,015 (GRCm39) T1087K probably benign Het
Tsc22d1 A G 14: 76,654,428 (GRCm39) probably null Het
Vmn2r52 T A 7: 9,893,169 (GRCm39) T657S probably damaging Het
Vmn2r63 A T 7: 42,576,314 (GRCm39) S500T probably benign Het
Vps45 A C 3: 95,953,725 (GRCm39) V209G probably benign Het
Xrcc1 C A 7: 24,269,905 (GRCm39) A442E probably benign Het
Ythdc1 T A 5: 86,970,704 (GRCm39) V430E probably damaging Het
Zfp422 A T 6: 116,603,623 (GRCm39) N125K probably damaging Het
Zfp53 T A 17: 21,725,263 (GRCm39) D58E possibly damaging Het
Zfp707 C T 15: 75,845,000 (GRCm39) Q66* probably null Het
Other mutations in Zfp341
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00499:Zfp341 APN 2 154,476,151 (GRCm39) missense probably damaging 1.00
IGL01352:Zfp341 APN 2 154,470,816 (GRCm39) missense probably benign 0.00
IGL01748:Zfp341 APN 2 154,470,847 (GRCm39) missense probably damaging 0.99
IGL02260:Zfp341 APN 2 154,483,969 (GRCm39) missense possibly damaging 0.89
IGL02329:Zfp341 APN 2 154,474,224 (GRCm39) missense possibly damaging 0.90
casanova_grimbacher UTSW 2 154,466,801 (GRCm39) missense probably damaging 1.00
Specious UTSW 2 154,488,054 (GRCm39) missense possibly damaging 0.63
R0079:Zfp341 UTSW 2 154,466,914 (GRCm39) nonsense probably null
R0570:Zfp341 UTSW 2 154,487,988 (GRCm39) missense probably benign 0.02
R0620:Zfp341 UTSW 2 154,476,193 (GRCm39) missense possibly damaging 0.94
R1018:Zfp341 UTSW 2 154,487,972 (GRCm39) missense probably damaging 1.00
R1611:Zfp341 UTSW 2 154,487,623 (GRCm39) missense probably damaging 1.00
R1733:Zfp341 UTSW 2 154,483,298 (GRCm39) missense probably benign 0.19
R1822:Zfp341 UTSW 2 154,488,054 (GRCm39) missense possibly damaging 0.63
R1956:Zfp341 UTSW 2 154,480,132 (GRCm39) missense probably benign 0.09
R2437:Zfp341 UTSW 2 154,470,721 (GRCm39) missense probably damaging 0.97
R3623:Zfp341 UTSW 2 154,466,801 (GRCm39) missense probably damaging 1.00
R4417:Zfp341 UTSW 2 154,470,907 (GRCm39) missense possibly damaging 0.94
R4807:Zfp341 UTSW 2 154,487,786 (GRCm39) utr 3 prime probably benign
R4863:Zfp341 UTSW 2 154,487,786 (GRCm39) utr 3 prime probably benign
R4955:Zfp341 UTSW 2 154,479,950 (GRCm39) missense probably damaging 0.98
R4962:Zfp341 UTSW 2 154,468,734 (GRCm39) missense possibly damaging 0.80
R5484:Zfp341 UTSW 2 154,466,921 (GRCm39) missense probably benign 0.00
R5864:Zfp341 UTSW 2 154,485,474 (GRCm39) missense possibly damaging 0.95
R5877:Zfp341 UTSW 2 154,474,209 (GRCm39) missense probably damaging 1.00
R5975:Zfp341 UTSW 2 154,472,361 (GRCm39) missense probably damaging 1.00
R5990:Zfp341 UTSW 2 154,487,579 (GRCm39) missense probably damaging 0.98
R6057:Zfp341 UTSW 2 154,466,954 (GRCm39) missense probably benign 0.01
R6882:Zfp341 UTSW 2 154,479,943 (GRCm39) missense probably damaging 1.00
R7686:Zfp341 UTSW 2 154,466,781 (GRCm39) missense probably damaging 0.96
R7701:Zfp341 UTSW 2 154,476,000 (GRCm39) splice site probably null
R7847:Zfp341 UTSW 2 154,476,114 (GRCm39) missense probably damaging 1.00
R8109:Zfp341 UTSW 2 154,469,820 (GRCm39) missense probably benign 0.04
R9313:Zfp341 UTSW 2 154,469,907 (GRCm39) missense probably damaging 1.00
R9484:Zfp341 UTSW 2 154,485,763 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- CTGTGCAGCAAGTCCTTCAG -3'
(R):5'- CTTGGATATAGACTGGGACGG -3'

Sequencing Primer
(F):5'- AGCAAGTCCTTCAGCCGTC -3'
(R):5'- AAGTGCCAGGTTGGAGCC -3'
Genotyping

Genotyping is performed by amplifying the region containing the mutation using PCR, followed by sequencing of the amplified region to detect the mutation.
 

PCR Primers

R48060007_PCR_F: 5’- CTGTGCAGCAAGTCCTTCAG-3’

R48060007_PCR_R: 5’- CTTGGATATAGACTGGGACGG-3’

Sequencing Primers

R48060007_SEQ_F: 5’- AGCAAGTCCTTCAGCCGTC-3’
 

R48060007_SEQ_R: 5’- AAGTGCCAGGTTGGAGCC-3’
 

PCR program

1) 94°C             2:00

2) 94°C             0:30

3) 55°C             0:30

4) 72°C             1:00

5) repeat steps (2-4) 40X

6) 72°C             10:00

7) 4°C               hold

The following sequence of 481 nucleotides is amplified (NCBI RefSeq: NC_000068, chromosome 1:154645647-154646127):

ctgtgcagca agtccttcag ccgtcgagct cacctggcag agcaccaacg agcccacaca       

ggcaactaca agttccgctg cgcgggctgc gccaagggct tctcacgaca caaatacctc      

aaggatcacc gctgccgcct gggccccacc aaggacaagg acctgcaagc ccggcggcct      

ccccggagga gggcaacggc gcgtggtggt ggcagcatcg ccagcgggca caaggaggag      

aaccccatgc cagacccgct ggggctggag gagctgaagg ccgcaggacc ctcgcctgag      

gctgcccctg gcaagccacc ctttgagcag gacgctgtgt tgtccattgt ggtgggcggg      

gacccggagc tggtggtgcc tgggcacaca gagggtctgg gctccaacct ggcacttgcc      

gagctgcagg ctggaaccga gggagggtgt gccatgctcg ccgtcccagt ctatatccaa      

g

Primer binding sites are underlined and the sequencing primer is highlighted; the mutated nucleotide is shown in red text (Chr. (+) = G>A).
Posted On 2016-07-28