Mutagenetix > Incidental Mutation

Incidental Mutation 'R0025:Wdfy3'

40766

Institutional Source

Beutler Lab

Gene Symbol

Wdfy3

Ensembl Gene

ENSMUSG00000043940

Gene Name

WD repeat and FYVE domain containing 3

Synonyms

2610509D04Rik, Ggtb3, Bchs, D5Ertd66e, Bwf1, Alfy

MMRRC Submission

038320-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.942) question?

Stock #

R0025 (G1)

Quality Score

118

Status

Validated

Chromosome

Chromosomal Location

101980822-102217787 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 101992912 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 3341 (D3341G)

Ref Sequence

ENSEMBL: ENSMUSP00000134244 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000053177] [ENSMUST00000174598] [ENSMUST00000212024]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000053177
AA Change: D3323G

PolyPhen 2 Score 0.960 (Sensitivity: 0.78; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000052607
Gene: ENSMUSG00000043940
AA Change: D3323G

Domain	Start	End	E-Value	Type
low complexity region	463	481	N/A	INTRINSIC
low complexity region	1408	1417	N/A	INTRINSIC
low complexity region	1629	1644	N/A	INTRINSIC
Pfam:PH_BEACH	2517	2638	3.1e-17	PFAM
Beach	2677	2958	2.54e-217	SMART
WD40	3054	3088	1.28e1	SMART
WD40	3098	3137	7.73e-6	SMART
WD40	3140	3178	8.29e-1	SMART
WD40	3183	3227	3.09e-1	SMART
low complexity region	3253	3274	N/A	INTRINSIC
low complexity region	3307	3318	N/A	INTRINSIC
WD40	3381	3420	1.33e1	SMART
FYVE	3428	3497	3.18e-27	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000173528

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000173955

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000173965

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000174312

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000174528

Predicted Effect

probably damaging
Transcript: ENSMUST00000174598
AA Change: D3341G

PolyPhen 2 Score 0.977 (Sensitivity: 0.76; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000134244
Gene: ENSMUSG00000043940
AA Change: D3341G

Domain	Start	End	E-Value	Type
low complexity region	463	481	N/A	INTRINSIC
Pfam:DUF4704	1392	1597	6.6e-11	PFAM
low complexity region	1629	1644	N/A	INTRINSIC
Pfam:PH_BEACH	2588	2656	1.8e-14	PFAM
Beach	2695	2976	2.54e-217	SMART
WD40	3072	3106	1.28e1	SMART
WD40	3116	3155	7.73e-6	SMART
WD40	3158	3196	8.29e-1	SMART
WD40	3201	3245	3.09e-1	SMART
low complexity region	3271	3292	N/A	INTRINSIC
low complexity region	3325	3336	N/A	INTRINSIC
WD40	3399	3438	1.33e1	SMART
FYVE	3446	3515	3.18e-27	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000212024
AA Change: D3327G

PolyPhen 2 Score 0.807 (Sensitivity: 0.84; Specificity: 0.93)

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000174864

Meta Mutation Damage Score

0.1500

Coding Region Coverage

1x: 99.0%
3x: 98.1%
10x: 95.7%
20x: 90.3%

Validation Efficiency

98% (115/117)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a phosphatidylinositol 3-phosphate-binding protein that functions as a master conductor for aggregate clearance by autophagy. This protein shuttles from the nuclear membrane to colocalize with aggregated proteins, where it complexes with other autophagic components to achieve macroautophagy-mediated clearance of these aggregated proteins. However, it is not necessary for starvation-induced macroautophagy. [provided by RefSeq, May 2010]
PHENOTYPE: Mice homozygous for hypomorphic mutations of this gene exhibit perinatal lethality, altered neural progenitor divisions and neuronal migration, a regionally enlarged cerebral cortex, and focal cortical dysplasias. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 106 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acsm1	A	T	7: 119,257,538 (GRCm39)	T435S	probably damaging	Het
Agtpbp1	G	A	13: 59,648,014 (GRCm39)	T602I	probably benign	Het
Ahnak2	T	A	12: 112,749,154 (GRCm39)	D231V	probably damaging	Het
Ampd3	G	A	7: 110,392,876 (GRCm39)	D215N	probably benign	Het
Ankrd17	T	C	5: 90,398,264 (GRCm39)	D1762G	probably damaging	Het
Asb8	C	T	15: 98,040,552 (GRCm39)	V37I	possibly damaging	Het
Bicra	T	C	7: 15,721,436 (GRCm39)	T694A	possibly damaging	Het
Btnl6	A	T	17: 34,733,273 (GRCm39)	M234K	probably benign	Het
Ccnb1	A	T	13: 100,916,289 (GRCm39)	V336D	probably damaging	Het
Cdca8	A	T	4: 124,815,047 (GRCm39)	L190Q	possibly damaging	Het
Cep290	A	T	10: 100,373,693 (GRCm39)	L1324F	probably damaging	Het
Ces1f	T	C	8: 93,998,513 (GRCm39)	E161G	probably benign	Het
Ces2g	A	G	8: 105,692,628 (GRCm39)		probably benign	Het
Cfap74	C	T	4: 155,510,572 (GRCm39)	R386C	probably benign	Het
Clec3b	A	G	9: 122,986,090 (GRCm39)	T163A	probably benign	Het
Cntnap4	T	G	8: 113,529,796 (GRCm39)	L668R	probably damaging	Het
Col27a1	A	G	4: 63,194,214 (GRCm39)	D857G	probably damaging	Het
Csf1	A	G	3: 107,655,960 (GRCm39)	V245A	probably benign	Het
Ctss	A	G	3: 95,457,448 (GRCm39)	Y302C	probably damaging	Het
Cyb5d1	A	G	11: 69,285,792 (GRCm39)		probably null	Het
Cyp1a2	A	G	9: 57,589,344 (GRCm39)	S157P	probably damaging	Het
Cyp2b9	A	T	7: 25,900,238 (GRCm39)	T349S	probably benign	Het
Dennd6b	T	C	15: 89,070,386 (GRCm39)	I428V	probably benign	Het
Denr	A	G	5: 124,065,298 (GRCm39)		probably benign	Het
Dnah9	G	A	11: 65,860,781 (GRCm39)		probably benign	Het
Dock3	G	T	9: 106,790,467 (GRCm39)	Q1419K	possibly damaging	Het
Dph3b-ps	A	T	13: 106,683,375 (GRCm39)		noncoding transcript	Het
Emc7	G	T	2: 112,289,830 (GRCm39)	D87Y	probably damaging	Het
Enah	T	C	1: 181,740,938 (GRCm39)	E462G	possibly damaging	Het
Eno1b	T	C	18: 48,180,806 (GRCm39)	I328T	probably benign	Het
Enpp6	A	G	8: 47,519,035 (GRCm39)	K268E	probably damaging	Het
Eps15l1	T	G	8: 73,135,341 (GRCm39)		probably benign	Het
Fam151a	T	C	4: 106,605,371 (GRCm39)	Y578H	probably benign	Het
Fmn2	T	C	1: 174,618,880 (GRCm39)	V1512A	probably damaging	Het
Focad	C	A	4: 88,327,196 (GRCm39)	N168K	probably benign	Het
Fyco1	A	G	9: 123,658,074 (GRCm39)	C701R	probably damaging	Het
Gabbr1	G	T	17: 37,378,102 (GRCm39)		probably benign	Het
Golga7b	A	T	19: 42,255,278 (GRCm39)	E76V	probably damaging	Het
Gucy2d	A	G	7: 98,116,959 (GRCm39)	D924G	probably benign	Het
H2-M9	A	G	17: 36,952,647 (GRCm39)	F133S	probably damaging	Het
Hc	A	G	2: 34,876,304 (GRCm39)	Y1581H	probably damaging	Het
Herc3	C	T	6: 58,851,293 (GRCm39)	P514L	probably damaging	Het
Hormad1	T	C	3: 95,492,436 (GRCm39)		probably benign	Het
Iigp1	T	A	18: 60,523,859 (GRCm39)	S326T	possibly damaging	Het
Itga2	G	A	13: 115,007,032 (GRCm39)	S432L	possibly damaging	Het
Kcnk7	T	G	19: 5,757,042 (GRCm39)	*344G	probably null	Het
Kif13a	A	G	13: 46,939,987 (GRCm39)		probably null	Het
Kif1a	A	C	1: 92,970,080 (GRCm39)	I1027S	probably damaging	Het
Kif2c	G	T	4: 117,022,714 (GRCm39)	H416Q	probably damaging	Het
Liat1	AGAGGAGGAGGAGGAGG	AGAGGAGGAGGAGG	11: 75,890,941 (GRCm39)		probably benign	Het
Map3k1	A	G	13: 111,892,663 (GRCm39)	V864A	probably benign	Het
Mark2	T	C	19: 7,263,287 (GRCm39)	D160G	probably damaging	Het
Mbd4	A	G	6: 115,821,529 (GRCm39)		probably null	Het
Micu1	A	G	10: 59,624,699 (GRCm39)		probably null	Het
Mink1	T	C	11: 70,503,868 (GRCm39)	W1263R	probably damaging	Het
Mov10	A	C	3: 104,711,919 (GRCm39)	L224R	probably damaging	Het
Ndel1	T	C	11: 68,726,999 (GRCm39)	E226G	probably damaging	Het
Neb	A	T	2: 52,112,786 (GRCm39)	V4336E	probably damaging	Het
Nln	T	A	13: 104,173,399 (GRCm39)	K602N	probably damaging	Het
Nlrp14	A	T	7: 106,780,465 (GRCm39)		probably benign	Het
Nmd3	A	T	3: 69,655,654 (GRCm39)	D445V	probably damaging	Het
Nop14	T	C	5: 34,801,297 (GRCm39)	I625V	probably benign	Het
Notch1	T	C	2: 26,360,943 (GRCm39)	Q1134R	probably damaging	Het
Nr4a2	T	C	2: 56,998,627 (GRCm39)	I392M	probably benign	Het
Or13n4	A	G	7: 106,422,963 (GRCm39)	F257L	possibly damaging	Het
Or4f6	T	A	2: 111,839,365 (GRCm39)	L55F	probably damaging	Het
Or8b57	A	G	9: 40,003,549 (GRCm39)	S234P	probably damaging	Het
Osbp	T	C	19: 11,961,322 (GRCm39)	Y454H	probably damaging	Het
Pak4	G	A	7: 28,263,708 (GRCm39)	R343C	probably damaging	Het
Pak5	T	C	2: 135,942,704 (GRCm39)	K479E	possibly damaging	Het
Pard3	C	A	8: 127,888,058 (GRCm39)	D73E	probably damaging	Het
Pcdh10	T	C	3: 45,334,934 (GRCm39)	V416A	possibly damaging	Het
Plek	A	C	11: 16,935,594 (GRCm39)	W261G	probably damaging	Het
Pmp22	A	T	11: 63,049,076 (GRCm39)		probably null	Het
Prph2	A	C	17: 47,230,697 (GRCm39)	K197Q	probably benign	Het
Prss45	T	A	9: 110,669,962 (GRCm39)	L257Q	probably damaging	Het
Psmb6	C	A	11: 70,417,171 (GRCm39)	H73Q	probably benign	Het
Rin2	T	C	2: 145,720,752 (GRCm39)		probably benign	Het
Rps6kb1	A	T	11: 86,402,413 (GRCm39)		probably null	Het
Scn10a	C	A	9: 119,499,550 (GRCm39)	D248Y	probably damaging	Het
Scn4a	C	T	11: 106,215,386 (GRCm39)	V1197I	probably benign	Het
Siglecf	A	T	7: 43,001,349 (GRCm39)	I106F	probably benign	Het
Sik1	A	G	17: 32,066,249 (GRCm39)		probably benign	Het
Slc22a21	T	G	11: 53,870,514 (GRCm39)	N57T	probably damaging	Het
Slc36a2	A	G	11: 55,053,621 (GRCm39)	L339P	probably damaging	Het
Slc4a9	G	T	18: 36,664,719 (GRCm39)		probably benign	Het
Smg1	G	A	7: 117,811,666 (GRCm39)	T104I	possibly damaging	Het
Stc2	A	T	11: 31,315,559 (GRCm39)		probably null	Het
Stx18	T	A	5: 38,249,908 (GRCm39)	Y74N	probably damaging	Het
Stxbp5	A	T	10: 9,638,492 (GRCm39)	H1102Q	probably damaging	Het
Tnfaip8l2	G	A	3: 95,047,339 (GRCm39)	L175F	probably damaging	Het
Tom1l2	T	C	11: 60,120,960 (GRCm39)	K450E	probably damaging	Het
Tpo	T	C	12: 30,150,389 (GRCm39)	Q497R	probably benign	Het
Tprg1l	G	T	4: 154,244,802 (GRCm39)		probably benign	Het
Triml2	A	G	8: 43,638,469 (GRCm39)	M146V	probably benign	Het
Tsc2	A	G	17: 24,849,978 (GRCm39)		probably benign	Het
Tut7	A	T	13: 59,953,142 (GRCm39)	D99E	probably benign	Het
Vit	G	A	17: 78,907,264 (GRCm39)	G229R	probably benign	Het
Vmn2r19	C	T	6: 123,308,506 (GRCm39)	L528F	probably benign	Het
Vwf	T	A	6: 125,659,775 (GRCm39)	I2658N	probably benign	Het
Wdr36	T	A	18: 32,992,360 (GRCm39)	D632E	probably damaging	Het
Wdr47	G	T	3: 108,545,307 (GRCm39)	A733S	probably damaging	Het
Zfp458	T	A	13: 67,405,962 (GRCm39)	H156L	probably damaging	Het
Zfp654	A	G	16: 64,605,181 (GRCm39)	V466A	probably benign	Het
Zfp804b	T	C	5: 6,821,665 (GRCm39)	E466G	probably damaging	Het
Zfp941	T	C	7: 140,393,185 (GRCm39)	D58G	probably benign	Het

Other mutations in Wdfy3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00332:Wdfy3	APN	5	102,063,204 (GRCm39)	critical splice donor site	probably null
IGL00567:Wdfy3	APN	5	102,059,896 (GRCm39)	splice site	probably benign
IGL01288:Wdfy3	APN	5	102,049,857 (GRCm39)	splice site	probably null
IGL01323:Wdfy3	APN	5	102,042,930 (GRCm39)	missense	probably damaging	1.00
IGL01352:Wdfy3	APN	5	102,091,986 (GRCm39)	missense	probably damaging	1.00
IGL01553:Wdfy3	APN	5	102,047,897 (GRCm39)	missense	probably benign
IGL01560:Wdfy3	APN	5	102,105,352 (GRCm39)	nonsense	probably null
IGL01566:Wdfy3	APN	5	102,044,454 (GRCm39)	splice site	probably benign
IGL01616:Wdfy3	APN	5	102,061,126 (GRCm39)	missense	probably damaging	0.97
IGL01630:Wdfy3	APN	5	102,055,354 (GRCm39)	missense	probably benign
IGL01791:Wdfy3	APN	5	102,085,278 (GRCm39)	missense	probably damaging	1.00
IGL01820:Wdfy3	APN	5	102,071,947 (GRCm39)	missense	probably benign	0.11
IGL01953:Wdfy3	APN	5	102,042,894 (GRCm39)	nonsense	probably null
IGL02121:Wdfy3	APN	5	102,046,376 (GRCm39)	missense	possibly damaging	0.85
IGL02167:Wdfy3	APN	5	102,109,023 (GRCm39)	missense	probably damaging	0.98
IGL02321:Wdfy3	APN	5	102,070,475 (GRCm39)	missense	probably damaging	0.99
IGL02327:Wdfy3	APN	5	102,036,058 (GRCm39)	missense	probably damaging	1.00
IGL02651:Wdfy3	APN	5	102,044,341 (GRCm39)	missense	probably benign	0.37
IGL02801:Wdfy3	APN	5	102,055,453 (GRCm39)	missense	probably damaging	1.00
IGL02839:Wdfy3	APN	5	102,116,786 (GRCm39)	missense	probably damaging	1.00
IGL02870:Wdfy3	APN	5	102,003,337 (GRCm39)	missense	probably damaging	1.00
IGL02997:Wdfy3	APN	5	102,042,778 (GRCm39)	missense	probably null	1.00
IGL03064:Wdfy3	APN	5	102,083,863 (GRCm39)	missense	probably damaging	0.99
IGL03090:Wdfy3	APN	5	102,014,142 (GRCm39)	missense	probably damaging	1.00
IGL03211:Wdfy3	APN	5	101,992,778 (GRCm39)	splice site	probably benign
IGL03237:Wdfy3	APN	5	101,992,465 (GRCm39)	missense	probably damaging	1.00
IGL03264:Wdfy3	APN	5	102,048,016 (GRCm39)	missense	probably damaging	1.00
Esurient	UTSW	5	102,091,969 (GRCm39)	missense	probably damaging	1.00
IGL02988:Wdfy3	UTSW	5	102,077,847 (GRCm39)	missense	probably damaging	0.99
PIT4382001:Wdfy3	UTSW	5	102,030,827 (GRCm39)	frame shift	probably null
R0010:Wdfy3	UTSW	5	101,996,215 (GRCm39)	missense	probably damaging	1.00
R0010:Wdfy3	UTSW	5	101,996,215 (GRCm39)	missense	probably damaging	1.00
R0031:Wdfy3	UTSW	5	102,037,161 (GRCm39)	missense	probably damaging	0.97
R0047:Wdfy3	UTSW	5	102,091,899 (GRCm39)	missense	probably damaging	1.00
R0047:Wdfy3	UTSW	5	102,091,899 (GRCm39)	missense	probably damaging	1.00
R0053:Wdfy3	UTSW	5	101,992,480 (GRCm39)	missense	probably damaging	0.97
R0078:Wdfy3	UTSW	5	102,035,971 (GRCm39)	missense	possibly damaging	0.57
R0147:Wdfy3	UTSW	5	102,065,277 (GRCm39)	missense	probably benign	0.05
R0148:Wdfy3	UTSW	5	102,065,277 (GRCm39)	missense	probably benign	0.05
R0279:Wdfy3	UTSW	5	102,015,958 (GRCm39)	missense	probably damaging	1.00
R0380:Wdfy3	UTSW	5	102,096,832 (GRCm39)	missense	probably damaging	0.99
R0472:Wdfy3	UTSW	5	102,105,309 (GRCm39)	missense	probably benign	0.13
R0513:Wdfy3	UTSW	5	102,038,655 (GRCm39)	missense	probably damaging	0.96
R0594:Wdfy3	UTSW	5	102,054,051 (GRCm39)	missense	possibly damaging	0.94
R0601:Wdfy3	UTSW	5	101,984,038 (GRCm39)	missense	probably benign
R0787:Wdfy3	UTSW	5	102,105,254 (GRCm39)	missense	probably damaging	1.00
R0825:Wdfy3	UTSW	5	102,017,917 (GRCm39)	missense	probably damaging	1.00
R1122:Wdfy3	UTSW	5	102,030,832 (GRCm39)	missense	possibly damaging	0.94
R1167:Wdfy3	UTSW	5	102,023,797 (GRCm39)	missense	probably benign
R1350:Wdfy3	UTSW	5	102,046,418 (GRCm39)	missense	probably damaging	1.00
R1422:Wdfy3	UTSW	5	102,032,080 (GRCm39)	splice site	probably benign
R1446:Wdfy3	UTSW	5	101,999,176 (GRCm39)	missense	possibly damaging	0.68
R1452:Wdfy3	UTSW	5	102,085,604 (GRCm39)	missense	possibly damaging	0.91
R1457:Wdfy3	UTSW	5	102,065,445 (GRCm39)	missense	possibly damaging	0.57
R1543:Wdfy3	UTSW	5	101,991,947 (GRCm39)	missense	probably benign
R1633:Wdfy3	UTSW	5	102,129,414 (GRCm39)	missense	probably damaging	1.00
R1643:Wdfy3	UTSW	5	102,023,781 (GRCm39)	missense	possibly damaging	0.62
R1656:Wdfy3	UTSW	5	102,089,313 (GRCm39)	missense	probably damaging	1.00
R1720:Wdfy3	UTSW	5	102,074,391 (GRCm39)	frame shift	probably null
R1743:Wdfy3	UTSW	5	101,991,931 (GRCm39)	missense	probably benign	0.12
R1745:Wdfy3	UTSW	5	102,096,795 (GRCm39)	missense	probably damaging	0.96
R1850:Wdfy3	UTSW	5	102,042,865 (GRCm39)	missense	probably damaging	1.00
R1852:Wdfy3	UTSW	5	102,063,242 (GRCm39)	missense	probably benign	0.00
R1854:Wdfy3	UTSW	5	102,036,052 (GRCm39)	missense	probably benign	0.05
R1880:Wdfy3	UTSW	5	102,065,301 (GRCm39)	missense	probably benign	0.05
R1930:Wdfy3	UTSW	5	102,089,358 (GRCm39)	missense	probably damaging	1.00
R1931:Wdfy3	UTSW	5	102,089,358 (GRCm39)	missense	probably damaging	1.00
R1956:Wdfy3	UTSW	5	102,067,275 (GRCm39)	missense	probably benign	0.30
R1965:Wdfy3	UTSW	5	102,099,178 (GRCm39)	missense	probably damaging	1.00
R1997:Wdfy3	UTSW	5	102,116,812 (GRCm39)	missense	probably damaging	1.00
R2015:Wdfy3	UTSW	5	102,008,352 (GRCm39)	missense	probably null	1.00
R2087:Wdfy3	UTSW	5	102,042,926 (GRCm39)	missense	probably damaging	1.00
R2156:Wdfy3	UTSW	5	102,046,291 (GRCm39)	critical splice donor site	probably null
R2192:Wdfy3	UTSW	5	102,055,408 (GRCm39)	missense	possibly damaging	0.55
R2313:Wdfy3	UTSW	5	102,037,150 (GRCm39)	missense	probably damaging	1.00
R2332:Wdfy3	UTSW	5	102,036,189 (GRCm39)	splice site	probably benign
R2406:Wdfy3	UTSW	5	102,036,125 (GRCm39)	missense	probably damaging	1.00
R2679:Wdfy3	UTSW	5	102,017,902 (GRCm39)	missense	probably damaging	1.00
R2857:Wdfy3	UTSW	5	102,023,796 (GRCm39)	missense	probably benign	0.04
R2937:Wdfy3	UTSW	5	102,091,988 (GRCm39)	missense	probably benign	0.07
R3765:Wdfy3	UTSW	5	102,009,266 (GRCm39)	missense	probably damaging	1.00
R3795:Wdfy3	UTSW	5	102,085,466 (GRCm39)	missense	probably damaging	1.00
R3937:Wdfy3	UTSW	5	102,092,105 (GRCm39)	nonsense	probably null
R3947:Wdfy3	UTSW	5	102,017,902 (GRCm39)	missense	probably damaging	1.00
R4024:Wdfy3	UTSW	5	102,071,961 (GRCm39)	splice site	probably benign
R4065:Wdfy3	UTSW	5	102,070,313 (GRCm39)	missense	probably benign	0.08
R4066:Wdfy3	UTSW	5	102,070,313 (GRCm39)	missense	probably benign	0.08
R4110:Wdfy3	UTSW	5	102,047,924 (GRCm39)	critical splice donor site	probably null
R4235:Wdfy3	UTSW	5	102,070,500 (GRCm39)	critical splice acceptor site	probably null
R4420:Wdfy3	UTSW	5	102,058,850 (GRCm39)	missense	probably damaging	0.97
R4620:Wdfy3	UTSW	5	102,054,011 (GRCm39)	missense	probably damaging	0.99
R4624:Wdfy3	UTSW	5	102,031,949 (GRCm39)	missense	possibly damaging	0.52
R4626:Wdfy3	UTSW	5	102,091,800 (GRCm39)	missense	probably damaging	1.00
R4727:Wdfy3	UTSW	5	102,077,894 (GRCm39)	missense	probably damaging	0.99
R4794:Wdfy3	UTSW	5	102,091,809 (GRCm39)	missense	probably damaging	1.00
R4869:Wdfy3	UTSW	5	102,042,787 (GRCm39)	missense	probably damaging	0.98
R4971:Wdfy3	UTSW	5	102,096,838 (GRCm39)	nonsense	probably null
R4973:Wdfy3	UTSW	5	102,090,985 (GRCm39)	missense	probably benign	0.00
R4976:Wdfy3	UTSW	5	102,090,985 (GRCm39)	missense	probably benign	0.00
R4984:Wdfy3	UTSW	5	102,090,985 (GRCm39)	missense	probably benign	0.00
R4986:Wdfy3	UTSW	5	102,090,985 (GRCm39)	missense	probably benign	0.00
R5068:Wdfy3	UTSW	5	102,042,803 (GRCm39)	missense	probably benign	0.15
R5105:Wdfy3	UTSW	5	102,003,415 (GRCm39)	missense	probably damaging	1.00
R5120:Wdfy3	UTSW	5	102,015,972 (GRCm39)	missense	possibly damaging	0.85
R5134:Wdfy3	UTSW	5	102,091,969 (GRCm39)	missense	probably damaging	1.00
R5139:Wdfy3	UTSW	5	101,997,133 (GRCm39)	critical splice donor site	probably null
R5235:Wdfy3	UTSW	5	101,994,972 (GRCm39)	missense	probably null	0.03
R5303:Wdfy3	UTSW	5	102,100,849 (GRCm39)	missense	probably damaging	1.00
R5368:Wdfy3	UTSW	5	102,020,724 (GRCm39)	missense	probably damaging	1.00
R5426:Wdfy3	UTSW	5	102,067,312 (GRCm39)	missense	probably damaging	0.97
R5442:Wdfy3	UTSW	5	102,044,425 (GRCm39)	missense	probably benign	0.04
R5487:Wdfy3	UTSW	5	101,984,140 (GRCm39)	missense	probably damaging	1.00
R5509:Wdfy3	UTSW	5	102,009,314 (GRCm39)	missense	possibly damaging	0.69
R5877:Wdfy3	UTSW	5	102,017,855 (GRCm39)	missense	probably damaging	1.00
R5988:Wdfy3	UTSW	5	102,032,004 (GRCm39)	missense	probably benign	0.00
R6017:Wdfy3	UTSW	5	101,999,225 (GRCm39)	missense	probably benign	0.01
R6019:Wdfy3	UTSW	5	101,997,289 (GRCm39)	missense	probably damaging	1.00
R6199:Wdfy3	UTSW	5	102,020,831 (GRCm39)	missense	possibly damaging	0.93
R6228:Wdfy3	UTSW	5	102,046,295 (GRCm39)	missense	possibly damaging	0.67
R6258:Wdfy3	UTSW	5	102,020,831 (GRCm39)	missense	possibly damaging	0.93
R6259:Wdfy3	UTSW	5	102,020,831 (GRCm39)	missense	possibly damaging	0.93
R6298:Wdfy3	UTSW	5	102,116,812 (GRCm39)	missense	probably damaging	1.00
R6479:Wdfy3	UTSW	5	102,061,045 (GRCm39)	missense	probably damaging	1.00
R6550:Wdfy3	UTSW	5	102,101,032 (GRCm39)	missense	probably benign	0.19
R6776:Wdfy3	UTSW	5	102,031,911 (GRCm39)	missense	possibly damaging	0.57
R6793:Wdfy3	UTSW	5	102,065,297 (GRCm39)	nonsense	probably null
R6809:Wdfy3	UTSW	5	102,071,813 (GRCm39)	missense	possibly damaging	0.63
R6836:Wdfy3	UTSW	5	102,100,865 (GRCm39)	missense	probably damaging	1.00
R6897:Wdfy3	UTSW	5	101,991,932 (GRCm39)	missense	probably benign	0.10
R7014:Wdfy3	UTSW	5	102,042,775 (GRCm39)	critical splice donor site	probably null
R7034:Wdfy3	UTSW	5	102,055,384 (GRCm39)	missense	probably damaging	1.00
R7035:Wdfy3	UTSW	5	102,003,415 (GRCm39)	missense	probably damaging	1.00
R7135:Wdfy3	UTSW	5	102,063,303 (GRCm39)	missense	probably damaging	1.00
R7182:Wdfy3	UTSW	5	102,091,758 (GRCm39)	missense	possibly damaging	0.51
R7217:Wdfy3	UTSW	5	102,049,785 (GRCm39)	missense	probably damaging	1.00
R7236:Wdfy3	UTSW	5	101,984,074 (GRCm39)	missense	probably damaging	0.99
R7264:Wdfy3	UTSW	5	102,003,389 (GRCm39)	missense	probably benign	0.02
R7418:Wdfy3	UTSW	5	102,105,366 (GRCm39)	missense	probably benign	0.08
R7533:Wdfy3	UTSW	5	102,030,354 (GRCm39)	missense	probably benign	0.27
R7543:Wdfy3	UTSW	5	102,083,925 (GRCm39)	missense	probably benign	0.00
R7625:Wdfy3	UTSW	5	102,003,252 (GRCm39)	splice site	probably null
R7788:Wdfy3	UTSW	5	101,996,223 (GRCm39)	missense	probably damaging	0.99
R7810:Wdfy3	UTSW	5	102,099,265 (GRCm39)	nonsense	probably null
R7810:Wdfy3	UTSW	5	102,042,940 (GRCm39)	missense	probably benign	0.01
R8204:Wdfy3	UTSW	5	102,000,451 (GRCm39)	missense	probably benign	0.00
R8268:Wdfy3	UTSW	5	102,089,476 (GRCm39)	missense	probably damaging	1.00
R8286:Wdfy3	UTSW	5	102,085,287 (GRCm39)	missense	probably benign
R8507:Wdfy3	UTSW	5	102,020,767 (GRCm39)	missense	probably benign	0.05
R8514:Wdfy3	UTSW	5	101,999,219 (GRCm39)	missense	possibly damaging	0.92
R8536:Wdfy3	UTSW	5	102,033,064 (GRCm39)	missense	probably benign
R8710:Wdfy3	UTSW	5	102,030,349 (GRCm39)	missense	probably damaging	1.00
R8735:Wdfy3	UTSW	5	102,077,951 (GRCm39)	missense	probably benign	0.00
R8749:Wdfy3	UTSW	5	102,030,446 (GRCm39)	missense	probably damaging	1.00
R8931:Wdfy3	UTSW	5	102,065,421 (GRCm39)	missense	probably benign	0.11
R8943:Wdfy3	UTSW	5	101,993,231 (GRCm39)	intron	probably benign
R8968:Wdfy3	UTSW	5	102,011,983 (GRCm39)	missense	probably benign	0.05
R8979:Wdfy3	UTSW	5	102,096,764 (GRCm39)	missense	probably damaging	1.00
R8998:Wdfy3	UTSW	5	101,993,058 (GRCm39)	missense	probably benign	0.05
R9045:Wdfy3	UTSW	5	101,995,040 (GRCm39)	missense	probably damaging	1.00
R9068:Wdfy3	UTSW	5	102,000,451 (GRCm39)	missense	probably benign	0.34
R9105:Wdfy3	UTSW	5	102,030,512 (GRCm39)	missense	probably benign	0.05
R9122:Wdfy3	UTSW	5	102,091,831 (GRCm39)	missense	probably damaging	1.00
R9209:Wdfy3	UTSW	5	102,078,830 (GRCm39)	missense	probably benign	0.01
R9249:Wdfy3	UTSW	5	101,996,359 (GRCm39)	missense	possibly damaging	0.82
R9348:Wdfy3	UTSW	5	102,089,358 (GRCm39)	missense	probably damaging	1.00
R9481:Wdfy3	UTSW	5	102,000,478 (GRCm39)	missense	probably benign	0.19
R9490:Wdfy3	UTSW	5	102,078,716 (GRCm39)	missense	probably benign	0.29
R9524:Wdfy3	UTSW	5	102,055,333 (GRCm39)	missense	probably benign	0.03
R9545:Wdfy3	UTSW	5	102,100,957 (GRCm39)	missense
R9548:Wdfy3	UTSW	5	102,033,059 (GRCm39)	missense	probably damaging	0.99
R9636:Wdfy3	UTSW	5	102,047,899 (GRCm39)	missense	probably benign
R9750:Wdfy3	UTSW	5	102,077,960 (GRCm39)	missense	probably benign	0.00
R9766:Wdfy3	UTSW	5	102,042,866 (GRCm39)	missense	possibly damaging	0.90
R9771:Wdfy3	UTSW	5	102,000,195 (GRCm39)	missense	probably damaging	1.00
Z1177:Wdfy3	UTSW	5	102,048,107 (GRCm39)	missense	probably benign	0.39

Predicted Primers

PCR Primer
(F):5'- TGCCTAGCCTGATCTATGGGAGAAC -3'
(R):5'- CCCAAGATGACGACAGCAGTGATTC -3'

Sequencing Primer
(F):5'- TCTTTGGCTCAGGCACAG -3'
(R):5'- CGACAGCAGTGATTCTGAAAC -3'

Posted On

2013-05-23