Mutagenetix > Incidental Mutation

Incidental Mutation 'R0066:Olfr169'

41120

Institutional Source

Beutler Lab

Gene Symbol

Olfr169

Ensembl Gene

ENSMUSG00000068535

Gene Name

olfactory receptor 169

Synonyms

GA_x54KRFPKG5P-16014972-16014031, MOR273-3P

MMRRC Submission

038357-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.057) question?

Stock #

R0066 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

19564889-19571809 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 19566049 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Phenylalanine at position 278 (Y278F)

Ref Sequence

ENSEMBL: ENSMUSP00000150528 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000090062] [ENSMUST00000215040] [ENSMUST00000215476] [ENSMUST00000216070]

AlphaFold

Q7TS53

Predicted Effect

probably damaging
Transcript: ENSMUST00000090062
AA Change: Y278F

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000087516
Gene: ENSMUSG00000068535
AA Change: Y278F

Domain	Start	End	E-Value	Type
Pfam:7tm_4	28	308	1.3e-45	PFAM
Pfam:7TM_GPCR_Srsx	35	303	2e-6	PFAM
Pfam:7tm_1	41	290	7.4e-29	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000215040
AA Change: Y278F

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Predicted Effect

probably damaging
Transcript: ENSMUST00000215476
AA Change: Y278F

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Predicted Effect

probably damaging
Transcript: ENSMUST00000216070
AA Change: Y278F

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Meta Mutation Damage Score

0.1277

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 96.3%
20x: 93.0%

Validation Efficiency

100% (107/107)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 104 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1810064F22Rik	A	G	9: 22,207,881		noncoding transcript	Het
5530400C23Rik	A	T	6: 133,292,324		probably benign	Het
Aco2	T	C	15: 81,903,465		probably benign	Het
Arap3	A	T	18: 37,996,707	S134T	probably benign	Het
Arsa	T	A	15: 89,474,336	M288L	possibly damaging	Het
Atg2b	A	T	12: 105,648,449	D1074E	probably benign	Het
Baiap2l1	A	T	5: 144,284,562	I174N	probably damaging	Het
Bpifb9a	A	T	2: 154,266,841	N421Y	possibly damaging	Het
Btn2a2	T	A	13: 23,478,485	I432L	probably benign	Het
Ccdc150	A	G	1: 54,356,691	I778V	probably benign	Het
Cd200r2	G	A	16: 44,909,674	V194I	possibly damaging	Het
Cep350	A	C	1: 155,911,218	L1421R	probably damaging	Het
Col24a1	G	A	3: 145,545,144	A1633T	probably damaging	Het
Col6a6	A	T	9: 105,702,213	C1938S	probably damaging	Het
Cspg4	A	T	9: 56,888,134	D1051V	probably damaging	Het
Cstf1	T	A	2: 172,373,056	N32K	probably benign	Het
Ctrb1	G	A	8: 111,686,637	R248*	probably null	Het
Cyp2d11	T	A	15: 82,391,757	M208L	probably benign	Het
Dbt	A	G	3: 116,543,829	Q334R	probably benign	Het
Dcaf12	A	G	4: 41,298,338	V270A	probably damaging	Het
Dis3l	T	A	9: 64,319,165	N361I	probably benign	Het
Dnah10	A	T	5: 124,763,076	D1315V	probably benign	Het
Dnah11	A	G	12: 118,126,886	F1080S	probably benign	Het
Dnm3	A	G	1: 162,407,361	V70A	probably damaging	Het
Dpy19l1	A	C	9: 24,414,409	M700R	possibly damaging	Het
Dpy19l2	G	A	9: 24,646,383		probably benign	Het
Dst	C	A	1: 34,189,553	H2254N	possibly damaging	Het
Epm2aip1	A	G	9: 111,272,463	N168S	probably benign	Het
Fchsd2	A	G	7: 101,278,424	Y691C	possibly damaging	Het
Fndc8	A	T	11: 82,897,572	D76V	probably benign	Het
Frmd4a	T	C	2: 4,473,152	L48P	probably damaging	Het
Gimap6	T	A	6: 48,702,470	I211F	probably damaging	Het
Gm15130	A	G	2: 111,138,939		probably benign	Het
Gm43302	T	A	5: 105,290,900	I41F	probably damaging	Het
Gm5698	C	T	1: 30,977,533	V146I	probably benign	Het
Gpatch1	G	A	7: 35,287,227	S768L	probably damaging	Het
Grb14	T	G	2: 64,938,492		probably null	Het
Hnrnpd	T	C	5: 99,964,701	E222G	probably damaging	Het
Il4ra	C	T	7: 125,576,231	P537L	possibly damaging	Het
Kalrn	T	C	16: 34,203,957	D610G	probably damaging	Het
Kcnh4	T	C	11: 100,757,800	H26R	probably benign	Het
Kctd2	T	G	11: 115,429,517		probably benign	Het
Khdrbs3	T	A	15: 68,995,037		probably benign	Het
Macf1	G	A	4: 123,432,150	Q3066*	probably null	Het
Mfn2	G	A	4: 147,885,445		probably benign	Het
Mmab	T	C	5: 114,436,465		probably benign	Het
Mrc1	T	C	2: 14,261,200	S310P	probably benign	Het
Mrps21	T	C	3: 95,862,885	Y44C	probably null	Het
Myh10	T	A	11: 68,699,491	F121Y	probably damaging	Het
Myo1f	A	G	17: 33,601,703	D840G	probably damaging	Het
Neb	T	A	2: 52,306,530	D553V	probably damaging	Het
Nol6	G	T	4: 41,119,572		probably benign	Het
Npr2	G	T	4: 43,632,329	V49L	probably benign	Het
Ntsr2	T	C	12: 16,654,119	I207T	probably benign	Het
Nwd1	T	A	8: 72,711,856	S1552T	probably benign	Het
Oas3	T	A	5: 120,758,875	I894F	probably damaging	Het
Olfr456	A	T	6: 42,486,935	M86K	probably benign	Het
Olfr736	T	A	14: 50,393,202	F149I	probably benign	Het
Olfr983	T	C	9: 40,092,687	N93S	possibly damaging	Het
Oprd1	A	G	4: 132,113,988	F220L	probably benign	Het
Pkd1l3	G	T	8: 109,620,471	G159C	unknown	Het
Plcb4	T	C	2: 135,961,769	S521P	probably benign	Het
Plcl1	A	T	1: 55,713,475	I993F	probably damaging	Het
Plcxd1	T	A	5: 110,101,502	V65E	probably damaging	Het
Plekha7	T	C	7: 116,157,508	S640G	probably damaging	Het
Ptprn2	A	C	12: 117,276,602	N993T	probably benign	Het
Rabepk	T	C	2: 34,795,306	D26G	possibly damaging	Het
Reck	A	G	4: 43,930,936	N646D	probably damaging	Het
Rfx2	A	T	17: 56,786,736		probably benign	Het
Ripk2	G	A	4: 16,123,868	Q436*	probably null	Het
Ryr1	C	T	7: 29,005,567		probably benign	Het
Sema6b	A	G	17: 56,128,271	V324A	possibly damaging	Het
Sik2	C	A	9: 50,998,533	M73I	probably benign	Het
Slc39a6	T	C	18: 24,599,269	K321E	probably damaging	Het
Slc7a4	C	A	16: 17,574,011	V520F	probably benign	Het
Slitrk6	A	T	14: 110,749,932	L781H	probably damaging	Het
Spink14	T	C	18: 44,028,763	V2A	probably benign	Het
Sptan1	C	A	2: 30,003,667		probably benign	Het
Stab1	C	T	14: 31,157,070		probably benign	Het
Tbc1d17	C	T	7: 44,844,071		probably benign	Het
Tbcd	T	A	11: 121,503,764	L49*	probably null	Het
Tmco6	A	G	18: 36,742,107	T477A	probably benign	Het
Tmem208	C	T	8: 105,328,225	A53V	probably benign	Het
Tpp2	A	G	1: 43,981,748	T837A	possibly damaging	Het
Tulp4	A	T	17: 6,201,733	N60I	probably damaging	Het
Ubqlnl	A	T	7: 104,148,938	W451R	probably damaging	Het
Usp53	G	T	3: 122,953,307	C363*	probably null	Het
Usp7	A	T	16: 8,691,418	H1017Q	probably benign	Het
Utp4	A	G	8: 106,922,898	T660A	possibly damaging	Het
Vmn1r194	A	T	13: 22,244,471	Y86F	probably benign	Het
Vmn1r195	A	T	13: 22,279,239	H293L	possibly damaging	Het
Vmn1r231	T	C	17: 20,889,736	R306G	probably benign	Het
Vmn2r63	T	C	7: 42,927,090		probably benign	Het
Vmn2r77	T	C	7: 86,800,756	V70A	probably benign	Het
Vmn2r85	T	C	10: 130,425,901	D189G	probably damaging	Het
Vps8	A	G	16: 21,477,523	E515G	possibly damaging	Het
Wdr18	C	A	10: 79,961,103	Y104*	probably null	Het
Wnk4	A	T	11: 101,265,435	D43V	probably damaging	Het
Xab2	A	T	8: 3,613,880	N346K	probably damaging	Het
Xirp2	T	C	2: 67,512,140	V1575A	possibly damaging	Het
Zdhhc12	C	T	2: 30,092,535	R50H	probably damaging	Het
Zdhhc8	A	G	16: 18,225,200	S379P	probably benign	Het
Zfp458	G	A	13: 67,259,609	Q58*	probably null	Het
Zfp747	A	T	7: 127,374,600	S133T	probably benign	Het

Other mutations in Olfr169

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01080:Olfr169	APN	16	19566208	missense	probably damaging	1.00
IGL01862:Olfr169	APN	16	19566676	missense	probably damaging	1.00
IGL02064:Olfr169	APN	16	19566548	missense	probably damaging	1.00
IGL03061:Olfr169	APN	16	19566713	missense	possibly damaging	0.87
IGL03136:Olfr169	APN	16	19566353	missense	probably damaging	1.00
R0243:Olfr169	UTSW	16	19566294	missense	probably damaging	0.97
R0629:Olfr169	UTSW	16	19565980	missense	possibly damaging	0.88
R1644:Olfr169	UTSW	16	19566406	missense	probably benign	0.11
R1943:Olfr169	UTSW	16	19566437	missense	probably benign	0.19
R3016:Olfr169	UTSW	16	19566391	missense	probably damaging	1.00
R4290:Olfr169	UTSW	16	19566244	missense	possibly damaging	0.88
R4689:Olfr169	UTSW	16	19566513	nonsense	probably null
R4791:Olfr169	UTSW	16	19566663	missense	possibly damaging	0.50
R5497:Olfr169	UTSW	16	19566330	missense	probably benign	0.10
R5843:Olfr169	UTSW	16	19566583	missense	probably damaging	1.00
R6106:Olfr169	UTSW	16	19566259	missense	probably damaging	0.99
R6249:Olfr169	UTSW	16	19565975	missense	probably damaging	0.99
R7895:Olfr169	UTSW	16	19566722	nonsense	probably null
R9284:Olfr169	UTSW	16	19566607	missense	probably damaging	1.00
R9335:Olfr169	UTSW	16	19566763	missense	probably benign	0.32
R9364:Olfr169	UTSW	16	19565972	missense	possibly damaging	0.68
R9404:Olfr169	UTSW	16	19565981	missense	probably benign	0.01
R9475:Olfr169	UTSW	16	19566520	missense	probably benign	0.09
R9554:Olfr169	UTSW	16	19565972	missense	possibly damaging	0.68

Predicted Primers

PCR Primer
(F):5'- GTGCAGCACCCATCAATAAGGACAA -3'
(R):5'- TGTGCAAATTCTCCTCACTGTATTCCAA -3'

Sequencing Primer
(F):5'- TTGCCTTACTACAGCATAGCAAG -3'
(R):5'- TTCCAAATGCAGTCATCAGGG -3'

Posted On

2013-05-23