Mutagenetix > Incidental Mutation

Incidental Mutation 'R0457:Zfp37'

41204

Institutional Source

Beutler Lab

Gene Symbol

Zfp37

Ensembl Gene

ENSMUSG00000028389

Gene Name

zinc finger protein 37

Synonyms

Zfp-37, Tzn

MMRRC Submission

038657-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.373) question?

Stock #

R0457 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

62107774-62127634 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 62109902 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Stop codon at position 387 (C387*)

Ref Sequence

ENSEMBL: ENSMUSP00000152400 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000068822] [ENSMUST00000129511] [ENSMUST00000220873] [ENSMUST00000221329] [ENSMUST00000222050] [ENSMUST00000222748]

AlphaFold

P17141

Predicted Effect

probably null
Transcript: ENSMUST00000068822
AA Change: C428*

SMART Domains

Protein: ENSMUSP00000070463
Gene: ENSMUSG00000028389
AA Change: C428*

Domain	Start	End	E-Value	Type
KRAB	13	62	5.89e-12	SMART
low complexity region	113	122	N/A	INTRINSIC
ZnF_C2H2	255	277	2.75e-3	SMART
ZnF_C2H2	283	305	3.95e-4	SMART
ZnF_C2H2	311	333	2.2e-2	SMART
ZnF_C2H2	339	361	8.02e-5	SMART
ZnF_C2H2	367	389	1.13e-4	SMART
ZnF_C2H2	395	417	9.88e-5	SMART
ZnF_C2H2	423	445	2.61e-4	SMART
ZnF_C2H2	451	473	6.08e-5	SMART
ZnF_C2H2	479	501	5.99e-4	SMART
ZnF_C2H2	507	529	5.99e-4	SMART
ZnF_C2H2	535	557	1.03e-2	SMART
ZnF_C2H2	563	585	9.88e-5	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000129511
AA Change: C74*

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000148664

Predicted Effect

probably null
Transcript: ENSMUST00000220873
AA Change: C387*

Predicted Effect

probably null
Transcript: ENSMUST00000221329
AA Change: C425*

Predicted Effect

probably null
Transcript: ENSMUST00000222050
AA Change: C424*

Predicted Effect

probably null
Transcript: ENSMUST00000222748
AA Change: C387*

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.4%
20x: 92.9%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a transcription factor that belongs to a large family of zinc finger proteins. A similar protein in mouse is thought to play a role in regulating the structures of the nucleolus and centromere in neurons. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Allele List at MGI

All alleles(4) : Gene trapped(4)

Other mutations in this stock

Total: 70 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aadacl2fm1	A	G	3: 59,844,054 (GRCm39)	I249M	possibly damaging	Het
Adcy5	T	C	16: 35,094,915 (GRCm39)	S691P	probably benign	Het
Ajm1	G	T	2: 25,468,358 (GRCm39)	R518S	possibly damaging	Het
Aspscr1	A	G	11: 120,568,444 (GRCm39)	E12G	probably benign	Het
Atp2a2	T	C	5: 122,607,777 (GRCm39)	Q244R	probably benign	Het
Birc6	A	G	17: 74,959,023 (GRCm39)	M3818V	probably benign	Het
Birc6	C	T	17: 74,969,620 (GRCm39)	A4230V	probably damaging	Het
Bub1b	T	C	2: 118,440,340 (GRCm39)	F148S	probably damaging	Het
C1ra	T	C	6: 124,499,712 (GRCm39)	S633P	probably benign	Het
Cacna2d1	A	G	5: 16,472,414 (GRCm39)	T274A	probably damaging	Het
Cmya5	A	G	13: 93,232,095 (GRCm39)	W998R	possibly damaging	Het
Crbn	T	C	6: 106,758,018 (GRCm39)	K404R	probably benign	Het
Cryga	T	C	1: 65,142,204 (GRCm39)	Y63C	probably damaging	Het
Csmd1	C	A	8: 16,551,407 (GRCm39)		probably null	Het
Defa-ps1	A	T	8: 22,185,758 (GRCm39)		noncoding transcript	Het
Dnajc10	T	A	2: 80,175,290 (GRCm39)	V559D	possibly damaging	Het
Dock1	A	T	7: 134,739,874 (GRCm39)	E1423D	possibly damaging	Het
Dpf3	A	T	12: 83,319,179 (GRCm39)	S44T	probably damaging	Het
Dyrk3	A	T	1: 131,064,094 (GRCm39)	V31D	possibly damaging	Het
F5	T	C	1: 164,021,769 (GRCm39)	S1415P	probably benign	Het
Fam186b	A	C	15: 99,169,166 (GRCm39)	I927S	probably benign	Het
Fcho1	C	T	8: 72,165,204 (GRCm39)	A418T	probably benign	Het
Fer1l6	G	A	15: 58,509,943 (GRCm39)		probably null	Het
Fndc7	G	T	3: 108,783,861 (GRCm39)	S249R	probably benign	Het
Ganab	A	G	19: 8,884,614 (GRCm39)	E139G	possibly damaging	Het
Gbp5	A	G	3: 142,213,518 (GRCm39)	D478G	probably damaging	Het
Gm17324	T	A	9: 78,355,580 (GRCm39)	M1K	probably null	Het
Gtpbp6	T	A	5: 110,254,608 (GRCm39)	R126S	probably damaging	Het
Hapln4	G	A	8: 70,541,122 (GRCm39)	W385*	probably null	Het
Hmcn2	T	A	2: 31,305,296 (GRCm39)		probably null	Het
Hsp90ab1	A	G	17: 45,879,914 (GRCm39)	V534A	probably damaging	Het
Kat6b	C	A	14: 21,720,598 (GRCm39)	T1650K	probably damaging	Het
Kpna1	T	A	16: 35,823,275 (GRCm39)	D42E	probably benign	Het
Lrrc14b	A	G	13: 74,509,279 (GRCm39)	M376T	probably benign	Het
Lrrc40	A	G	3: 157,760,201 (GRCm39)		probably null	Het
Ltv1	T	C	10: 13,067,887 (GRCm39)	T34A	probably benign	Het
Mga	T	A	2: 119,746,969 (GRCm39)	N373K	probably damaging	Het
Msh3	A	T	13: 92,357,505 (GRCm39)	M101K	probably damaging	Het
Mthfd2l	T	C	5: 91,168,065 (GRCm39)	M320T	possibly damaging	Het
Mug1	G	A	6: 121,838,514 (GRCm39)	E506K	probably benign	Het
Ngb	T	C	12: 87,147,503 (GRCm39)	D54G	probably damaging	Het
Ntrk1	A	G	3: 87,699,014 (GRCm39)	F84L	probably benign	Het
Or1j18	A	T	2: 36,624,545 (GRCm39)	I71F	probably benign	Het
Or52n2b	T	A	7: 104,566,180 (GRCm39)	T108S	probably benign	Het
Phf12	T	A	11: 77,908,994 (GRCm39)	I358N	possibly damaging	Het
Plec	A	G	15: 76,061,801 (GRCm39)	F2577S	probably damaging	Het
Polr1c	T	A	17: 46,558,689 (GRCm39)	Y36F	probably benign	Het
Prkd1	A	T	12: 50,413,155 (GRCm39)	M672K	probably damaging	Het
Prob1	T	C	18: 35,785,539 (GRCm39)	Y905C	probably damaging	Het
Ptpn23	T	A	9: 110,215,361 (GRCm39)	H1433L	possibly damaging	Het
Rnf11	A	T	4: 109,314,149 (GRCm39)	L80Q	probably damaging	Het
Sbp	G	A	17: 24,164,286 (GRCm39)	G183D	probably benign	Het
Scgb2b7	A	T	7: 31,403,437 (GRCm39)	C90S	possibly damaging	Het
Slc4a9	T	C	18: 36,668,471 (GRCm39)	L710P	probably damaging	Het
Spire1	T	A	18: 67,685,670 (GRCm39)	I35F	probably damaging	Het
Sptbn2	T	C	19: 4,795,966 (GRCm39)	V1715A	possibly damaging	Het
St7	T	C	6: 17,819,281 (GRCm39)	F62L	probably damaging	Het
Svep1	C	T	4: 58,118,136 (GRCm39)	G862D	probably damaging	Het
Syne1	A	T	10: 4,972,041 (GRCm39)	M8789K	probably damaging	Het
Synpo2	A	G	3: 122,906,421 (GRCm39)	L965P	probably damaging	Het
Trhde	A	T	10: 114,284,167 (GRCm39)	M772K	probably benign	Het
Ttn	T	A	2: 76,608,851 (GRCm39)	K15976*	probably null	Het
Unc13a	A	C	8: 72,110,645 (GRCm39)		probably null	Het
Vcan	T	C	13: 89,851,318 (GRCm39)	E1214G	possibly damaging	Het
Vmn1r29	T	C	6: 58,285,072 (GRCm39)	V264A	probably benign	Het
Vmn1r60	T	A	7: 5,548,118 (GRCm39)		probably benign	Het
Wdr90	C	T	17: 26,079,459 (GRCm39)	R225H	probably benign	Het
Wnk1	G	A	6: 119,946,293 (GRCm39)	T620I	probably damaging	Het
Zan	C	T	5: 137,405,968 (GRCm39)		probably benign	Het
Zfp521	T	C	18: 13,977,897 (GRCm39)	T839A	probably benign	Het

Other mutations in Zfp37

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02742:Zfp37	APN	4	62,110,548 (GRCm39)	missense	possibly damaging	0.69
B6584:Zfp37	UTSW	4	62,109,615 (GRCm39)	missense	probably damaging	1.00
R0383:Zfp37	UTSW	4	62,110,122 (GRCm39)	start codon destroyed	probably null	0.61
R1258:Zfp37	UTSW	4	62,110,054 (GRCm39)	missense	probably damaging	1.00
R2072:Zfp37	UTSW	4	62,109,945 (GRCm39)	missense	probably damaging	1.00
R2073:Zfp37	UTSW	4	62,109,945 (GRCm39)	missense	probably damaging	1.00
R2261:Zfp37	UTSW	4	62,109,873 (GRCm39)	missense	probably damaging	1.00
R2262:Zfp37	UTSW	4	62,109,873 (GRCm39)	missense	probably damaging	1.00
R2507:Zfp37	UTSW	4	62,109,493 (GRCm39)	missense	probably damaging	1.00
R2897:Zfp37	UTSW	4	62,110,014 (GRCm39)	missense	probably damaging	1.00
R2898:Zfp37	UTSW	4	62,110,014 (GRCm39)	missense	probably damaging	1.00
R3826:Zfp37	UTSW	4	62,110,800 (GRCm39)	missense	probably benign	0.00
R3879:Zfp37	UTSW	4	62,109,572 (GRCm39)	nonsense	probably null
R4034:Zfp37	UTSW	4	62,109,933 (GRCm39)	missense	probably damaging	1.00
R4491:Zfp37	UTSW	4	62,110,365 (GRCm39)	missense	probably benign	0.07
R4823:Zfp37	UTSW	4	62,109,740 (GRCm39)	missense	probably benign	0.18
R5186:Zfp37	UTSW	4	62,109,493 (GRCm39)	missense	probably damaging	1.00
R5650:Zfp37	UTSW	4	62,110,002 (GRCm39)	missense	probably damaging	1.00
R5886:Zfp37	UTSW	4	62,109,471 (GRCm39)	missense	probably damaging	1.00
R5925:Zfp37	UTSW	4	62,109,450 (GRCm39)	missense	possibly damaging	0.47
R7050:Zfp37	UTSW	4	62,109,908 (GRCm39)	missense	possibly damaging	0.72
R7553:Zfp37	UTSW	4	62,110,236 (GRCm39)	missense	probably damaging	1.00
R7583:Zfp37	UTSW	4	62,110,253 (GRCm39)	start gained	probably benign
R7646:Zfp37	UTSW	4	62,109,532 (GRCm39)	missense	probably damaging	0.99
R8061:Zfp37	UTSW	4	62,109,665 (GRCm39)	nonsense	probably null
R8076:Zfp37	UTSW	4	62,109,553 (GRCm39)	missense	possibly damaging	0.67
R8477:Zfp37	UTSW	4	62,110,240 (GRCm39)	missense	probably damaging	1.00
R8756:Zfp37	UTSW	4	62,110,371 (GRCm39)	missense	possibly damaging	0.94
R9000:Zfp37	UTSW	4	62,126,651 (GRCm39)	missense	unknown
R9362:Zfp37	UTSW	4	62,110,299 (GRCm39)	missense	probably benign	0.11
R9400:Zfp37	UTSW	4	62,109,904 (GRCm39)	missense	probably damaging	1.00
R9745:Zfp37	UTSW	4	62,110,644 (GRCm39)	missense	possibly damaging	0.58
X0026:Zfp37	UTSW	4	62,123,326 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- CAAACCAGTCAGTTGTCAGGTTTCCTA -3'
(R):5'- GAAATCACACTGCCGTACTCCATCTAAA -3'

Sequencing Primer
(F):5'- ctttcccacattcaacacactc -3'
(R):5'- cagccagaagtcccacc -3'

Posted On

2013-05-23