|Institutional Source||Beutler Lab|
|Gene Name||cell division cycle 45|
|Is this an essential gene?||Essential (E-score: 1.000)|
|Stock #||R0458 (G1)|
|Chromosomal Location||18780447-18811987 bp(-) (GRCm38)|
|Type of Mutation||splice site|
|DNA Base Change (assembly)||T to A at 18781972 bp (GRCm38)|
|Amino Acid Change|
|Ref Sequence||ENSEMBL: ENSMUSP00000094753 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000000028] [ENSMUST00000043577] [ENSMUST00000096990]|
|Coding Region Coverage||
|Validation Efficiency||100% (79/79)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
PHENOTYPE: Homozygous mutant embryos do not develop after implantation, resulting in embryonic lethality between E4.5-E5.5. Heterozygous animals appear normal and fertile. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Cdc45||
(F):5'- ACCGTGTTCTGATGGGAAACACAAG -3'
(R):5'- ATGCTGCCAGCCATCGTTTACC -3'
(F):5'- CAAGTAAACGCCTCTGTTGAG -3'
(R):5'- AGGATAAGGCTCTGGACTCTC -3'