Mutagenetix > Incidental Mutation

Incidental Mutation 'R0463:Scn7a'

41449

Institutional Source

Beutler Lab

Gene Symbol

Scn7a

Ensembl Gene

ENSMUSG00000034810

Gene Name

sodium channel, voltage-gated, type VII, alpha

Synonyms

NaG, Nav2, Nav2.3, Nax, Scn6a

MMRRC Submission

038663-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.087) question?

Stock #

R0463 (G1)

Quality Score

223

Status

Not validated

Chromosome

Chromosomal Location

66673425-66784914 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 66675740 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Arginine at position 1602 (G1602R)

Ref Sequence

ENSEMBL: ENSMUSP00000042405 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000042792]

AlphaFold

B1AYL1

Predicted Effect

probably benign
Transcript: ENSMUST00000042792
AA Change: G1602R

PolyPhen 2 Score 0.049 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000042405
Gene: ENSMUSG00000034810
AA Change: G1602R

Domain	Start	End	E-Value	Type
Pfam:Ion_trans	118	405	4.7e-53	PFAM
coiled coil region	415	443	N/A	INTRINSIC
Pfam:Ion_trans	505	739	5.8e-36	PFAM
Pfam:Na_trans_assoc	741	929	4.1e-17	PFAM
Pfam:Ion_trans	933	1204	3e-49	PFAM
Pfam:Ion_trans	1250	1505	5e-37	PFAM
IQ	1624	1646	6.4e-2	SMART

Coding Region Coverage

1x: 99.2%
3x: 98.4%
10x: 96.5%
20x: 93.5%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes one of the many voltage-gated sodium channel proteins. For proper functioning of neurons and muscles during action potentials, voltage-gated sodium channels direct sodium ion diffusion for membrane depolarization. This sodium channel protein has some atypical characteristics; the similarity between the human and mouse proteins is lower compared to other orthologous sodium channel pairs. Also, the S4 segments, which sense voltage changes, have fewer positive charged residues that in other sodium channels; domain 4 has fewer arginine and lysine residues compared to other sodium channel proteins. Several alternatively spliced transcript variants exist, but the full-length natures of all of them remain unknown. [provided by RefSeq, Dec 2011]
PHENOTYPE: Mice homozygous for disruptions in this gene have a modified dietary preference for NaCl but are phenotypically normal otherwise. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 92 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930522L14Rik	A	G	5: 109,737,060 (GRCm38)		probably benign	Het
Abcd2	C	T	15: 91,159,124 (GRCm38)	M620I	probably benign	Het
Ada	T	A	2: 163,730,351 (GRCm38)	I243F	probably benign	Het
Adam12	T	C	7: 133,974,416 (GRCm38)		probably null	Het
Adarb2	A	T	13: 8,203,188 (GRCm38)		probably benign	Het
Adk	A	C	14: 21,423,536 (GRCm38)	Q287P	probably benign	Het
Ahnak	A	G	19: 9,009,407 (GRCm38)		probably benign	Het
Aoc3	C	T	11: 101,331,606 (GRCm38)	R223W	probably damaging	Het
Aqp11	T	C	7: 97,729,021 (GRCm38)	D229G	probably benign	Het
Arhgap28	A	G	17: 67,896,225 (GRCm38)	S78P	probably damaging	Het
Bfsp2	T	A	9: 103,426,655 (GRCm38)	E383D	possibly damaging	Het
Bmpr1b	A	T	3: 141,857,430 (GRCm38)	V251D	possibly damaging	Het
Calhm1	C	T	19: 47,143,841 (GRCm38)	V112I	probably benign	Het
Catsperd	A	G	17: 56,659,554 (GRCm38)	D508G	probably damaging	Het
Cfap54	A	G	10: 92,874,943 (GRCm38)		probably null	Het
Cfap70	A	T	14: 20,448,563 (GRCm38)	Y19N	probably damaging	Het
Chga	A	T	12: 102,562,951 (GRCm38)	R396*	probably null	Het
Cntnap3	T	C	13: 64,778,876 (GRCm38)	E560G	probably damaging	Het
Csmd1	T	C	8: 15,921,759 (GRCm38)	T3024A	probably damaging	Het
Csrnp1	CCCTCCTCCTCCTCCTCCTC	CCCTCCTCCTCCTCCTC	9: 119,972,775 (GRCm38)		probably benign	Het
Cysltr1	A	G	X: 106,578,655 (GRCm38)	V75A	possibly damaging	Het
Dnaaf11	T	A	15: 66,380,474 (GRCm38)	M448L	probably benign	Het
Dnah2	A	T	11: 69,423,126 (GRCm38)	M4140K	probably damaging	Het
Dph5	A	G	3: 115,928,703 (GRCm38)	S277G	probably benign	Het
Eftud2	A	T	11: 102,864,771 (GRCm38)	D203E	probably damaging	Het
Egf	A	G	3: 129,706,233 (GRCm38)	Y252H	probably benign	Het
Egf	A	G	3: 129,737,549 (GRCm38)	S126P	probably damaging	Het
Faf1	C	T	4: 109,890,941 (GRCm38)	A481V	probably benign	Het
Fat2	A	T	11: 55,262,829 (GRCm38)	V3519D	probably damaging	Het
Fbln7	C	A	2: 128,877,511 (GRCm38)	A76E	probably benign	Het
Galnt1	A	T	18: 24,254,525 (GRCm38)	K49N	probably benign	Het
Glb1	ACCC	ACC	9: 114,421,744 (GRCm38)		probably null	Het
Grk1	T	C	8: 13,409,279 (GRCm38)	Y277H	probably damaging	Het
Hap1	A	G	11: 100,349,305 (GRCm38)	L555P	probably damaging	Het
Ier3	T	C	17: 35,822,108 (GRCm38)	I94T	possibly damaging	Het
Il11	T	C	7: 4,776,024 (GRCm38)	T36A	probably damaging	Het
Il5ra	A	T	6: 106,731,890 (GRCm38)	D296E	probably damaging	Het
Itk	A	T	11: 46,331,989 (GRCm38)	V551E	probably damaging	Het
Kcna2	T	A	3: 107,105,160 (GRCm38)	D352E	probably benign	Het
Kif5a	A	T	10: 127,235,652 (GRCm38)	S776T	probably benign	Het
Klrb1c	T	C	6: 128,780,403 (GRCm38)	E233G	probably benign	Het
Kpna7	T	C	5: 145,007,994 (GRCm38)	K12R	possibly damaging	Het
Lhpp	C	T	7: 132,610,677 (GRCm38)		probably benign	Het
Lhx8	A	T	3: 154,328,171 (GRCm38)		probably null	Het
Magel2	T	A	7: 62,378,030 (GRCm38)	H227Q	possibly damaging	Het
Man1a	A	G	10: 54,074,498 (GRCm38)	V176A	probably damaging	Het
Mapkbp1	T	A	2: 120,023,151 (GRCm38)	M1152K	probably benign	Het
Mcoln3	T	A	3: 146,140,576 (GRCm38)	L547*	probably null	Het
Myof	T	C	19: 37,916,504 (GRCm38)	D1624G	probably damaging	Het
Myom2	T	C	8: 15,104,123 (GRCm38)	V687A	probably benign	Het
Nav1	C	A	1: 135,452,207 (GRCm38)	V1586F	possibly damaging	Het
Ndufb8	T	C	19: 44,550,345 (GRCm38)	E179G	possibly damaging	Het
Nfam1	T	C	15: 83,001,483 (GRCm38)	T223A	probably damaging	Het
Nrcam	T	A	12: 44,551,341 (GRCm38)	V371E	probably damaging	Het
Nup210l	A	G	3: 90,180,211 (GRCm38)	Q1097R	probably null	Het
Obox5	T	A	7: 15,757,646 (GRCm38)	M37K	probably damaging	Het
Obscn	A	T	11: 59,061,530 (GRCm38)	N4270K	probably benign	Het
Or4d2	G	A	11: 87,893,196 (GRCm38)	H243Y	probably damaging	Het
Or5ak25	T	A	2: 85,438,286 (GRCm38)	S291C	probably damaging	Het
Or6c1	A	G	10: 129,681,839 (GRCm38)	M300T	probably benign	Het
Or8c15	G	A	9: 38,209,064 (GRCm38)	A2T	probably benign	Het
Or8k16	T	C	2: 85,689,839 (GRCm38)	S137P	possibly damaging	Het
Patj	G	A	4: 98,674,308 (GRCm38)	E1505K	probably damaging	Het
Pnliprp1	T	A	19: 58,738,196 (GRCm38)	Y328*	probably null	Het
Ppp1r36	G	A	12: 76,418,967 (GRCm38)	E43K	probably damaging	Het
Ptch1	C	T	13: 63,520,307 (GRCm38)	V939I	probably damaging	Het
Rgs22	C	A	15: 36,092,938 (GRCm38)	K396N	probably damaging	Het
Rsrc1	A	T	3: 67,180,861 (GRCm38)	H176L	probably damaging	Het
Ryr3	A	T	2: 112,661,701 (GRCm38)	F3743L	probably damaging	Het
Sftpc	A	T	14: 70,522,670 (GRCm38)	V49E	probably damaging	Het
Slc16a10	A	G	10: 40,040,616 (GRCm38)	V430A	probably benign	Het
Slco4c1	A	C	1: 96,867,920 (GRCm38)	S138A	possibly damaging	Het
Snd1	T	C	6: 28,724,956 (GRCm38)	I501T	probably benign	Het
Stxbp2	T	A	8: 3,632,559 (GRCm38)	D49E	probably damaging	Het
Sytl4	A	T	X: 133,962,187 (GRCm38)	D16E	probably benign	Het
Tbc1d9b	G	A	11: 50,145,067 (GRCm38)	G130E	probably benign	Het
Tdrd6	T	A	17: 43,625,561 (GRCm38)	D1532V	probably damaging	Het
Tekt1	T	C	11: 72,351,952 (GRCm38)	D243G	probably damaging	Het
Tet2	A	G	3: 133,486,666 (GRCm38)	L669S	possibly damaging	Het
Tnnt3	A	G	7: 142,512,335 (GRCm38)	N201S	probably benign	Het
Trdn	A	G	10: 33,466,421 (GRCm38)		probably null	Het
Trim36	T	C	18: 46,178,456 (GRCm38)	E259G	possibly damaging	Het
Trpm1	C	T	7: 64,220,254 (GRCm38)	P436S	probably benign	Het
Vmn1r183	T	A	7: 24,055,501 (GRCm38)	L243Q	probably damaging	Het
Vps13b	T	C	15: 35,597,409 (GRCm38)	S1032P	probably damaging	Het
Vps37d	T	C	5: 135,076,541 (GRCm38)	E76G	probably damaging	Het
Vps72	A	G	3: 95,121,304 (GRCm38)	H202R	probably benign	Het
Wdr75	T	C	1: 45,819,602 (GRCm38)	S644P	probably damaging	Het
Wrn	T	A	8: 33,280,815 (GRCm38)	E697V	possibly damaging	Het
Xirp2	A	G	2: 67,514,918 (GRCm38)	D2501G	probably benign	Het
Zfp472	T	C	17: 32,975,962 (GRCm38)	W24R	probably damaging	Het
Zmym6	T	C	4: 127,122,772 (GRCm38)	V782A	probably damaging	Het

Other mutations in Scn7a

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00090:Scn7a	APN	2	66,683,327 (GRCm38)	splice site	probably benign
IGL00432:Scn7a	APN	2	66,741,982 (GRCm38)	nonsense	probably null
IGL00720:Scn7a	APN	2	66,676,044 (GRCm38)	missense	possibly damaging	0.67
IGL00783:Scn7a	APN	2	66,692,564 (GRCm38)	missense	probably damaging	0.99
IGL00784:Scn7a	APN	2	66,692,564 (GRCm38)	missense	probably damaging	0.99
IGL00926:Scn7a	APN	2	66,684,131 (GRCm38)	missense	probably benign	0.06
IGL00963:Scn7a	APN	2	66,703,945 (GRCm38)	splice site	probably benign
IGL01099:Scn7a	APN	2	66,684,238 (GRCm38)	missense	probably damaging	1.00
IGL01326:Scn7a	APN	2	66,752,260 (GRCm38)	missense	probably benign	0.13
IGL01538:Scn7a	APN	2	66,703,852 (GRCm38)	missense	probably benign
IGL01624:Scn7a	APN	2	66,751,925 (GRCm38)	missense	probably benign	0.07
IGL01794:Scn7a	APN	2	66,675,509 (GRCm38)	missense	probably benign
IGL02100:Scn7a	APN	2	66,675,499 (GRCm38)	makesense	probably null
IGL02326:Scn7a	APN	2	66,700,048 (GRCm38)	missense	probably benign	0.00
IGL02472:Scn7a	APN	2	66,752,314 (GRCm38)	missense	probably damaging	1.00
IGL02528:Scn7a	APN	2	66,700,175 (GRCm38)	missense	probably damaging	1.00
IGL02798:Scn7a	APN	2	66,713,875 (GRCm38)	missense	probably benign	0.00
IGL03026:Scn7a	APN	2	66,676,098 (GRCm38)	missense	probably damaging	0.99
IGL03071:Scn7a	APN	2	66,699,947 (GRCm38)	missense	possibly damaging	0.89
IGL03080:Scn7a	APN	2	66,697,816 (GRCm38)	missense	probably benign	0.01
IGL03180:Scn7a	APN	2	66,676,234 (GRCm38)	missense	possibly damaging	0.94
IGL03337:Scn7a	APN	2	66,675,960 (GRCm38)	missense	probably benign	0.00
alert	UTSW	2	66,680,246 (GRCm38)	nonsense	probably null
glimmer	UTSW	2	66,743,703 (GRCm38)	missense	probably damaging	0.96
Uptick	UTSW	2	66,700,049 (GRCm38)	nonsense	probably null
PIT4514001:Scn7a	UTSW	2	66,684,179 (GRCm38)	missense	probably damaging	1.00
R0004:Scn7a	UTSW	2	66,687,795 (GRCm38)	missense	possibly damaging	0.81
R0076:Scn7a	UTSW	2	66,714,037 (GRCm38)	missense	probably benign	0.04
R0230:Scn7a	UTSW	2	66,726,284 (GRCm38)	missense	probably damaging	1.00
R0846:Scn7a	UTSW	2	66,697,600 (GRCm38)	missense	possibly damaging	0.71
R1237:Scn7a	UTSW	2	66,680,295 (GRCm38)	missense	probably damaging	0.98
R1282:Scn7a	UTSW	2	66,700,849 (GRCm38)	missense	probably damaging	0.98
R1467:Scn7a	UTSW	2	66,689,558 (GRCm38)	missense	probably benign	0.01
R1467:Scn7a	UTSW	2	66,689,558 (GRCm38)	missense	probably benign	0.01
R1501:Scn7a	UTSW	2	66,700,163 (GRCm38)	missense	probably benign	0.37
R1672:Scn7a	UTSW	2	66,697,600 (GRCm38)	missense	possibly damaging	0.71
R1690:Scn7a	UTSW	2	66,675,943 (GRCm38)	missense	probably damaging	0.99
R1712:Scn7a	UTSW	2	66,705,103 (GRCm38)	missense	probably benign	0.05
R1758:Scn7a	UTSW	2	66,700,887 (GRCm38)	missense	probably damaging	0.97
R1758:Scn7a	UTSW	2	66,680,183 (GRCm38)	missense	probably benign	0.00
R1775:Scn7a	UTSW	2	66,680,955 (GRCm38)	missense	probably benign	0.02
R1848:Scn7a	UTSW	2	66,684,013 (GRCm38)	critical splice donor site	probably null
R1851:Scn7a	UTSW	2	66,680,291 (GRCm38)	missense	probably benign
R1919:Scn7a	UTSW	2	66,699,973 (GRCm38)	missense	probably damaging	1.00
R1932:Scn7a	UTSW	2	66,676,102 (GRCm38)	missense	probably damaging	1.00
R1945:Scn7a	UTSW	2	66,675,980 (GRCm38)	missense	probably damaging	1.00
R1970:Scn7a	UTSW	2	66,684,289 (GRCm38)	missense	possibly damaging	0.89
R1998:Scn7a	UTSW	2	66,683,269 (GRCm38)	missense	probably damaging	0.99
R2008:Scn7a	UTSW	2	66,687,747 (GRCm38)	missense	possibly damaging	0.82
R2038:Scn7a	UTSW	2	66,737,436 (GRCm38)	missense	probably damaging	1.00
R2113:Scn7a	UTSW	2	66,675,968 (GRCm38)	missense	probably damaging	1.00
R2128:Scn7a	UTSW	2	66,697,986 (GRCm38)	missense	probably damaging	0.99
R2163:Scn7a	UTSW	2	66,675,956 (GRCm38)	missense	probably damaging	0.97
R2421:Scn7a	UTSW	2	66,726,302 (GRCm38)	splice site	probably benign
R2446:Scn7a	UTSW	2	66,692,658 (GRCm38)	missense	probably damaging	0.98
R2922:Scn7a	UTSW	2	66,700,207 (GRCm38)	splice site	probably benign
R3015:Scn7a	UTSW	2	66,699,896 (GRCm38)	missense	probably benign	0.08
R3034:Scn7a	UTSW	2	66,682,808 (GRCm38)	missense	probably damaging	1.00
R3419:Scn7a	UTSW	2	66,700,895 (GRCm38)	frame shift	probably null
R3429:Scn7a	UTSW	2	66,700,895 (GRCm38)	frame shift	probably null
R3430:Scn7a	UTSW	2	66,700,895 (GRCm38)	frame shift	probably null
R3434:Scn7a	UTSW	2	66,675,503 (GRCm38)	missense	probably benign	0.01
R3803:Scn7a	UTSW	2	66,680,246 (GRCm38)	nonsense	probably null
R3831:Scn7a	UTSW	2	66,697,684 (GRCm38)	missense	probably damaging	0.96
R3833:Scn7a	UTSW	2	66,697,684 (GRCm38)	missense	probably damaging	0.96
R4017:Scn7a	UTSW	2	66,741,985 (GRCm38)	missense	probably damaging	1.00
R4244:Scn7a	UTSW	2	66,742,001 (GRCm38)	missense	probably benign	0.00
R4245:Scn7a	UTSW	2	66,742,001 (GRCm38)	missense	probably benign	0.00
R4276:Scn7a	UTSW	2	66,684,063 (GRCm38)	missense	probably damaging	0.97
R4307:Scn7a	UTSW	2	66,675,755 (GRCm38)	missense	possibly damaging	0.47
R4327:Scn7a	UTSW	2	66,737,471 (GRCm38)	missense	probably damaging	1.00
R4353:Scn7a	UTSW	2	66,676,436 (GRCm38)	missense	probably benign	0.00
R4721:Scn7a	UTSW	2	66,684,185 (GRCm38)	missense	probably damaging	1.00
R4722:Scn7a	UTSW	2	66,700,884 (GRCm38)	missense	possibly damaging	0.95
R4781:Scn7a	UTSW	2	66,703,760 (GRCm38)	missense	possibly damaging	0.95
R4792:Scn7a	UTSW	2	66,726,248 (GRCm38)	missense	probably damaging	1.00
R5362:Scn7a	UTSW	2	66,699,998 (GRCm38)	missense	probably damaging	1.00
R5437:Scn7a	UTSW	2	66,676,346 (GRCm38)	missense	probably damaging	1.00
R5729:Scn7a	UTSW	2	66,741,957 (GRCm38)	critical splice donor site	probably null
R5777:Scn7a	UTSW	2	66,692,569 (GRCm38)	missense	probably damaging	1.00
R5785:Scn7a	UTSW	2	66,697,568 (GRCm38)	missense	possibly damaging	0.79
R5821:Scn7a	UTSW	2	66,743,703 (GRCm38)	missense	probably damaging	0.96
R5830:Scn7a	UTSW	2	66,714,051 (GRCm38)	nonsense	probably null
R5877:Scn7a	UTSW	2	66,699,873 (GRCm38)	nonsense	probably null
R5881:Scn7a	UTSW	2	66,675,526 (GRCm38)	missense	probably benign	0.01
R5967:Scn7a	UTSW	2	66,675,713 (GRCm38)	missense	probably damaging	1.00
R5988:Scn7a	UTSW	2	66,726,214 (GRCm38)	nonsense	probably null
R6077:Scn7a	UTSW	2	66,697,596 (GRCm38)	missense	probably damaging	1.00
R6135:Scn7a	UTSW	2	66,703,900 (GRCm38)	missense	probably benign
R6242:Scn7a	UTSW	2	66,700,766 (GRCm38)	missense	probably benign	0.00
R6264:Scn7a	UTSW	2	66,675,526 (GRCm38)	missense	possibly damaging	0.93
R6291:Scn7a	UTSW	2	66,700,114 (GRCm38)	missense	probably damaging	0.98
R6544:Scn7a	UTSW	2	66,684,100 (GRCm38)	missense	probably damaging	1.00
R6770:Scn7a	UTSW	2	66,729,184 (GRCm38)	splice site	probably null
R6997:Scn7a	UTSW	2	66,703,803 (GRCm38)	missense	probably damaging	1.00
R7014:Scn7a	UTSW	2	66,741,959 (GRCm38)	missense	probably null	1.00
R7126:Scn7a	UTSW	2	66,757,286 (GRCm38)	missense	possibly damaging	0.80
R7129:Scn7a	UTSW	2	66,700,193 (GRCm38)	missense	probably benign	0.14
R7176:Scn7a	UTSW	2	66,676,288 (GRCm38)	missense	probably damaging	1.00
R7185:Scn7a	UTSW	2	66,687,795 (GRCm38)	missense	possibly damaging	0.81
R7276:Scn7a	UTSW	2	66,757,162 (GRCm38)	missense	probably damaging	1.00
R7332:Scn7a	UTSW	2	66,692,554 (GRCm38)	nonsense	probably null
R7421:Scn7a	UTSW	2	66,675,532 (GRCm38)	missense	probably benign	0.07
R7488:Scn7a	UTSW	2	66,757,230 (GRCm38)	missense	probably benign	0.16
R7636:Scn7a	UTSW	2	66,743,828 (GRCm38)	missense	possibly damaging	0.67
R7685:Scn7a	UTSW	2	66,676,192 (GRCm38)	missense	probably damaging	1.00
R7711:Scn7a	UTSW	2	66,700,877 (GRCm38)	missense	probably damaging	1.00
R7813:Scn7a	UTSW	2	66,676,345 (GRCm38)	missense	probably damaging	1.00
R7833:Scn7a	UTSW	2	66,676,150 (GRCm38)	missense	probably damaging	1.00
R7914:Scn7a	UTSW	2	66,699,950 (GRCm38)	missense	probably damaging	0.97
R7953:Scn7a	UTSW	2	66,757,326 (GRCm38)	missense	possibly damaging	0.90
R7970:Scn7a	UTSW	2	66,675,829 (GRCm38)	missense	probably damaging	1.00
R8061:Scn7a	UTSW	2	66,692,594 (GRCm38)	missense	probably damaging	1.00
R8121:Scn7a	UTSW	2	66,700,859 (GRCm38)	missense	probably damaging	1.00
R8172:Scn7a	UTSW	2	66,675,847 (GRCm38)	missense	possibly damaging	0.90
R8209:Scn7a	UTSW	2	66,700,860 (GRCm38)	missense	possibly damaging	0.88
R8226:Scn7a	UTSW	2	66,700,860 (GRCm38)	missense	possibly damaging	0.88
R8288:Scn7a	UTSW	2	66,675,974 (GRCm38)	missense	probably damaging	1.00
R8431:Scn7a	UTSW	2	66,703,820 (GRCm38)	missense	possibly damaging	0.62
R8678:Scn7a	UTSW	2	66,743,697 (GRCm38)	splice site	probably benign
R8745:Scn7a	UTSW	2	66,680,182 (GRCm38)	missense	probably benign
R8781:Scn7a	UTSW	2	66,737,431 (GRCm38)	missense	probably benign	0.03
R8848:Scn7a	UTSW	2	66,700,049 (GRCm38)	nonsense	probably null
R8878:Scn7a	UTSW	2	66,675,855 (GRCm38)	missense	probably damaging	1.00
R8943:Scn7a	UTSW	2	66,694,862 (GRCm38)	synonymous	silent
R8991:Scn7a	UTSW	2	66,684,244 (GRCm38)	missense	possibly damaging	0.65
R9147:Scn7a	UTSW	2	66,684,163 (GRCm38)	missense	possibly damaging	0.89
R9148:Scn7a	UTSW	2	66,684,163 (GRCm38)	missense	possibly damaging	0.89
R9402:Scn7a	UTSW	2	66,680,112 (GRCm38)	missense	probably damaging	1.00
R9501:Scn7a	UTSW	2	66,752,235 (GRCm38)	missense	probably benign	0.00
R9546:Scn7a	UTSW	2	66,752,259 (GRCm38)	missense	possibly damaging	0.93
R9715:Scn7a	UTSW	2	66,689,558 (GRCm38)	missense	possibly damaging	0.93
X0060:Scn7a	UTSW	2	66,689,682 (GRCm38)	missense	probably benign	0.01
X0066:Scn7a	UTSW	2	66,680,192 (GRCm38)	missense	probably benign
Z1088:Scn7a	UTSW	2	66,713,951 (GRCm38)	missense	probably damaging	0.98
Z1177:Scn7a	UTSW	2	66,752,269 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TCGATTTTGCCAGAGTGAGCACC -3'
(R):5'- AGGTTTGACCCTGATAGGACCCAG -3'

Sequencing Primer
(F):5'- TGCCAGAGTGAGCACCTTTAG -3'
(R):5'- CAGTACATAGACTCTAGCAAGCTTTC -3'

Posted On

2013-05-23