Mutagenetix > Incidental Mutation

Incidental Mutation 'IGL03263:Washc2'

414987

Institutional Source

Australian Phenomics Network (link to record)

Gene Symbol

Washc2

Ensembl Gene

ENSMUSG00000024104

Gene Name

WASH complex subunit 2

Synonyms

C530005J20Rik, D6Wsu116e, Fam21

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

IGL03263

Quality Score

Status

Chromosome

Chromosomal Location

116184988-116239632 bp(+) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

T to A at 116215084 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000144982 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000036759] [ENSMUST00000204283] [ENSMUST00000204476]

AlphaFold

Q6PGL7

Predicted Effect

probably benign
Transcript: ENSMUST00000036759

SMART Domains

Protein: ENSMUSP00000038983
Gene: ENSMUSG00000024104

Domain	Start	End	E-Value	Type
coiled coil region	95	129	N/A	INTRINSIC
low complexity region	196	213	N/A	INTRINSIC
low complexity region	216	238	N/A	INTRINSIC
low complexity region	262	276	N/A	INTRINSIC
low complexity region	443	455	N/A	INTRINSIC
low complexity region	531	561	N/A	INTRINSIC
low complexity region	660	668	N/A	INTRINSIC
low complexity region	801	810	N/A	INTRINSIC
Pfam:CAP-ZIP_m	939	1074	4.3e-58	PFAM
low complexity region	1268	1282	N/A	INTRINSIC
low complexity region	1297	1317	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000203802

Predicted Effect

probably benign
Transcript: ENSMUST00000204283

SMART Domains

Protein: ENSMUSP00000144703
Gene: ENSMUSG00000024104

Domain	Start	End	E-Value	Type
coiled coil region	9	43	N/A	INTRINSIC
low complexity region	110	127	N/A	INTRINSIC
low complexity region	130	152	N/A	INTRINSIC
low complexity region	176	190	N/A	INTRINSIC
low complexity region	357	369	N/A	INTRINSIC
low complexity region	445	475	N/A	INTRINSIC
low complexity region	574	582	N/A	INTRINSIC
low complexity region	715	724	N/A	INTRINSIC
Pfam:CAP-ZIP_m	853	988	2.8e-55	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000204476

SMART Domains

Protein: ENSMUSP00000144982
Gene: ENSMUSG00000024104

Domain	Start	End	E-Value	Type
coiled coil region	95	129	N/A	INTRINSIC
low complexity region	196	213	N/A	INTRINSIC
low complexity region	216	238	N/A	INTRINSIC
low complexity region	262	276	N/A	INTRINSIC
low complexity region	443	455	N/A	INTRINSIC
low complexity region	531	561	N/A	INTRINSIC
low complexity region	660	668	N/A	INTRINSIC
low complexity region	801	810	N/A	INTRINSIC

Coding Region Coverage

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 48 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700123K08Rik	T	A	5: 138,562,499 (GRCm39)	I81F	probably damaging	Het
Acacb	C	T	5: 114,351,754 (GRCm39)	H1164Y	probably damaging	Het
Adamts5	A	T	16: 85,666,830 (GRCm39)	V554E	probably damaging	Het
Asns	G	A	6: 7,689,404 (GRCm39)	R33C	probably benign	Het
BC051076	T	G	5: 88,111,977 (GRCm39)		noncoding transcript	Het
Bcas3	C	T	11: 85,712,948 (GRCm39)		probably benign	Het
Bpifb1	A	G	2: 154,057,226 (GRCm39)	M395V	probably benign	Het
Cdcp1	C	A	9: 123,009,152 (GRCm39)	V509L	probably benign	Het
Cdhr2	A	G	13: 54,865,926 (GRCm39)	T277A	possibly damaging	Het
Ces1d	C	A	8: 93,896,346 (GRCm39)		probably null	Het
Clca4a	T	A	3: 144,672,192 (GRCm39)	E250V	probably damaging	Het
Cplx4	T	C	18: 66,100,559 (GRCm39)	D79G	probably benign	Het
Dcaf11	A	G	14: 55,802,949 (GRCm39)	D246G	probably damaging	Het
Dnah2	A	T	11: 69,420,207 (GRCm39)		probably null	Het
Dock3	A	T	9: 106,807,330 (GRCm39)		probably benign	Het
Fam170a	T	C	18: 50,413,588 (GRCm39)		probably benign	Het
Fgfr2	T	A	7: 129,782,149 (GRCm39)	M423L	probably benign	Het
Gabra2	A	G	5: 71,130,836 (GRCm39)	F331L	probably damaging	Het
Galnt18	C	T	7: 111,119,321 (GRCm39)	R400Q	probably damaging	Het
H2-M11	A	T	17: 36,859,805 (GRCm39)	Q266L	probably damaging	Het
Igf2bp1	A	T	11: 95,857,499 (GRCm39)	V502D	probably damaging	Het
Ik	A	G	18: 36,881,699 (GRCm39)	N111S	probably damaging	Het
Intu	T	A	3: 40,627,027 (GRCm39)	Y269*	probably null	Het
Krt82	T	C	15: 101,450,307 (GRCm39)	Y463C	probably benign	Het
Lrig1	A	G	6: 94,588,628 (GRCm39)	M507T	probably benign	Het
Lrrc37	A	G	11: 103,504,525 (GRCm39)	V2481A	possibly damaging	Het
Mag	T	G	7: 30,598,953 (GRCm39)		probably null	Het
Map7	T	A	10: 20,121,068 (GRCm39)	Y121*	probably null	Het
Marchf6	A	G	15: 31,486,508 (GRCm39)	I349T	probably benign	Het
Mas1	A	G	17: 13,060,451 (GRCm39)	V324A	possibly damaging	Het
Nckap1l	T	C	15: 103,372,832 (GRCm39)	W259R	probably damaging	Het
Nhsl1	T	A	10: 18,373,827 (GRCm39)	Y164*	probably null	Het
Or52e5	T	A	7: 104,719,209 (GRCm39)	H178Q	probably damaging	Het
Or8b38	A	T	9: 37,973,009 (GRCm39)	Y131F	probably damaging	Het
Pcdhb2	A	G	18: 37,429,059 (GRCm39)	D344G	probably damaging	Het
Pclo	T	C	5: 14,731,824 (GRCm39)	V3442A	unknown	Het
Polr3c	C	T	3: 96,621,567 (GRCm39)		probably benign	Het
Ppp2r2d	C	T	7: 138,474,651 (GRCm39)	R11*	probably null	Het
Ptgfr	T	A	3: 151,541,500 (GRCm39)	M3L	probably benign	Het
Rfx6	A	G	10: 51,601,903 (GRCm39)	S741G	probably benign	Het
Rif1	T	C	2: 51,980,273 (GRCm39)	V490A	probably damaging	Het
Samd7	T	A	3: 30,816,302 (GRCm39)	H349Q	probably damaging	Het
Sh3pxd2a	T	C	19: 47,302,482 (GRCm39)	N199S	probably damaging	Het
Spef2	T	A	15: 9,667,305 (GRCm39)	K794N	possibly damaging	Het
Spta1	C	T	1: 174,041,484 (GRCm39)	A1316V	probably damaging	Het
Vwa7	A	G	17: 35,240,575 (GRCm39)	E410G	probably benign	Het
Wdr72	A	C	9: 74,064,711 (GRCm39)	Y581S	probably damaging	Het
Zdhhc17	T	C	10: 110,796,877 (GRCm39)	D298G	probably damaging	Het

Other mutations in Washc2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00422:Washc2	APN	6	116,233,637 (GRCm39)	missense	probably benign
IGL00552:Washc2	APN	6	116,233,785 (GRCm39)	missense	possibly damaging	0.85
IGL01397:Washc2	APN	6	116,224,959 (GRCm39)	missense	probably benign	0.44
IGL01668:Washc2	APN	6	116,239,299 (GRCm39)	missense	probably damaging	1.00
IGL01982:Washc2	APN	6	116,213,150 (GRCm39)	missense	probably benign	0.22
IGL02022:Washc2	APN	6	116,236,126 (GRCm39)	missense	probably benign	0.22
IGL02150:Washc2	APN	6	116,208,593 (GRCm39)	splice site	probably benign
IGL02224:Washc2	APN	6	116,197,530 (GRCm39)	missense	possibly damaging	0.70
IGL02553:Washc2	APN	6	116,218,571 (GRCm39)	missense	probably damaging	0.98
IGL02555:Washc2	APN	6	116,186,061 (GRCm39)	missense	probably damaging	1.00
IGL02612:Washc2	APN	6	116,197,577 (GRCm39)	missense	possibly damaging	0.90
IGL02622:Washc2	APN	6	116,190,979 (GRCm39)	splice site	probably benign
IGL02900:Washc2	APN	6	116,204,435 (GRCm39)	missense	probably damaging	1.00
fading	UTSW	6	116,231,114 (GRCm39)	missense	probably damaging	1.00
R0218:Washc2	UTSW	6	116,225,007 (GRCm39)	nonsense	probably null
R0285:Washc2	UTSW	6	116,198,800 (GRCm39)	missense	probably damaging	1.00
R0346:Washc2	UTSW	6	116,197,484 (GRCm39)	splice site	probably benign
R0677:Washc2	UTSW	6	116,221,577 (GRCm39)	missense	probably damaging	1.00
R0919:Washc2	UTSW	6	116,185,225 (GRCm39)	missense	probably damaging	1.00
R1144:Washc2	UTSW	6	116,201,495 (GRCm39)	missense	probably damaging	1.00
R1666:Washc2	UTSW	6	116,200,215 (GRCm39)	critical splice donor site	probably null
R1687:Washc2	UTSW	6	116,233,673 (GRCm39)	missense	probably benign	0.06
R1702:Washc2	UTSW	6	116,206,267 (GRCm39)	missense	probably damaging	0.99
R1740:Washc2	UTSW	6	116,208,593 (GRCm39)	splice site	probably benign
R1952:Washc2	UTSW	6	116,232,052 (GRCm39)	missense	possibly damaging	0.92
R1964:Washc2	UTSW	6	116,185,948 (GRCm39)	missense	probably damaging	0.99
R2039:Washc2	UTSW	6	116,201,400 (GRCm39)	missense	probably damaging	0.99
R3084:Washc2	UTSW	6	116,204,454 (GRCm39)	missense	probably benign	0.00
R3552:Washc2	UTSW	6	116,197,529 (GRCm39)	missense	probably damaging	1.00
R3790:Washc2	UTSW	6	116,224,933 (GRCm39)	splice site	probably benign
R3949:Washc2	UTSW	6	116,185,165 (GRCm39)	utr 5 prime	probably benign
R4089:Washc2	UTSW	6	116,233,253 (GRCm39)	splice site	probably null
R4133:Washc2	UTSW	6	116,235,891 (GRCm39)	missense	probably damaging	0.99
R4258:Washc2	UTSW	6	116,185,202 (GRCm39)	missense	probably damaging	1.00
R4510:Washc2	UTSW	6	116,197,517 (GRCm39)	missense	probably damaging	1.00
R4511:Washc2	UTSW	6	116,197,517 (GRCm39)	missense	probably damaging	1.00
R4613:Washc2	UTSW	6	116,206,230 (GRCm39)	missense	probably damaging	1.00
R4614:Washc2	UTSW	6	116,215,135 (GRCm39)	missense	possibly damaging	0.83
R4794:Washc2	UTSW	6	116,235,610 (GRCm39)	missense	probably benign	0.03
R5224:Washc2	UTSW	6	116,185,965 (GRCm39)	makesense	probably null
R5367:Washc2	UTSW	6	116,236,111 (GRCm39)	missense	probably damaging	1.00
R5602:Washc2	UTSW	6	116,225,056 (GRCm39)	missense	possibly damaging	0.73
R6013:Washc2	UTSW	6	116,231,114 (GRCm39)	missense	probably damaging	1.00
R6075:Washc2	UTSW	6	116,204,327 (GRCm39)	missense	probably benign	0.02
R6086:Washc2	UTSW	6	116,233,177 (GRCm39)	splice site	probably null
R6344:Washc2	UTSW	6	116,235,719 (GRCm39)	missense	probably benign	0.08
R6593:Washc2	UTSW	6	116,236,210 (GRCm39)	missense	probably damaging	1.00
R7048:Washc2	UTSW	6	116,197,544 (GRCm39)	missense	possibly damaging	0.96
R7062:Washc2	UTSW	6	116,196,949 (GRCm39)	missense	possibly damaging	0.72
R7241:Washc2	UTSW	6	116,185,168 (GRCm39)	start codon destroyed	probably null	0.01
R7283:Washc2	UTSW	6	116,204,379 (GRCm39)	missense	probably damaging	0.99
R7681:Washc2	UTSW	6	116,237,618 (GRCm39)	missense	probably damaging	0.99
R7810:Washc2	UTSW	6	116,236,020 (GRCm39)	missense	probably benign
R7908:Washc2	UTSW	6	116,225,106 (GRCm39)	missense	probably benign
R7923:Washc2	UTSW	6	116,203,385 (GRCm39)	missense	possibly damaging	0.83
R8067:Washc2	UTSW	6	116,201,464 (GRCm39)	missense	probably damaging	0.99
R8224:Washc2	UTSW	6	116,218,457 (GRCm39)	missense	probably damaging	0.99
R8697:Washc2	UTSW	6	116,206,220 (GRCm39)	missense	probably benign	0.17
R8841:Washc2	UTSW	6	116,235,916 (GRCm39)	missense	probably benign	0.16
R8920:Washc2	UTSW	6	116,221,615 (GRCm39)	missense	possibly damaging	0.92
R8971:Washc2	UTSW	6	116,231,399 (GRCm39)	missense	probably damaging	0.99
R9010:Washc2	UTSW	6	116,198,823 (GRCm39)	missense	probably damaging	1.00
R9231:Washc2	UTSW	6	116,235,899 (GRCm39)	missense	probably benign	0.05
R9257:Washc2	UTSW	6	116,193,069 (GRCm39)	nonsense	probably null
R9361:Washc2	UTSW	6	116,239,433 (GRCm39)	makesense	probably null
R9571:Washc2	UTSW	6	116,237,631 (GRCm39)	critical splice donor site	probably null
R9764:Washc2	UTSW	6	116,186,048 (GRCm39)	missense	possibly damaging	0.96
X0018:Washc2	UTSW	6	116,185,219 (GRCm39)	missense	probably null	0.96

Posted On

2016-08-02