Mutagenetix > Incidental Mutation

Incidental Mutation 'R0467:Nomo1'

41693

Institutional Source

Beutler Lab

Gene Symbol

Nomo1

Ensembl Gene

ENSMUSG00000030835

Gene Name

nodal modulator 1

Synonyms

D7Ertd156e, Nomo, PM5

MMRRC Submission

038667-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.700) question?

Stock #

R0467 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

45683122-45733636 bp(+) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

T to A at 45721911 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000033121 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000033121]

AlphaFold

Q6GQT9

Predicted Effect

probably null
Transcript: ENSMUST00000033121

SMART Domains

Protein: ENSMUSP00000033121
Gene: ENSMUSG00000030835

Domain	Start	End	E-Value	Type
low complexity region	2	21	N/A	INTRINSIC
internal_repeat_1	22	215	2.35e-7	PROSPERO
Pfam:CarboxypepD_reg	322	395	3.5e-12	PFAM
Pfam:DUF2012	331	401	5.7e-10	PFAM
low complexity region	709	732	N/A	INTRINSIC
low complexity region	881	893	N/A	INTRINSIC
Blast:FN3	913	1017	6e-22	BLAST
low complexity region	1156	1164	N/A	INTRINSIC
low complexity region	1203	1214	N/A	INTRINSIC

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.6%
3x: 98.8%
10x: 96.7%
20x: 93.3%

Validation Efficiency

98% (52/53)

Allele List at MGI

All alleles(8) : Gene trapped(8)

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca17	A	G	17: 24,532,151 (GRCm39)		probably benign	Het
Anapc1	A	G	2: 128,510,963 (GRCm39)	I511T	probably damaging	Het
Atf6	A	T	1: 170,621,589 (GRCm39)	H477Q	probably damaging	Het
C4b	A	G	17: 34,955,101 (GRCm39)	V795A	probably benign	Het
Cdh26	C	T	2: 178,123,425 (GRCm39)	R675C	possibly damaging	Het
Cdk12	T	C	11: 98,094,405 (GRCm39)	V71A	probably damaging	Het
Cul3	A	T	1: 80,258,580 (GRCm39)	D419E	probably benign	Het
Ddi2	A	G	4: 141,412,495 (GRCm39)	I139T	probably benign	Het
Dnaaf1	T	A	8: 120,317,471 (GRCm39)	D333E	probably benign	Het
Dnase1	A	G	16: 3,857,013 (GRCm39)	D7G	probably damaging	Het
G3bp1	T	C	11: 55,389,452 (GRCm39)	F383L	probably damaging	Het
Galc	A	C	12: 98,208,904 (GRCm39)	I250R	probably damaging	Het
Garin1b	G	A	6: 29,326,606 (GRCm39)	S241N	probably damaging	Het
Gcfc2	T	C	6: 81,900,863 (GRCm39)	V59A	possibly damaging	Het
Gm6133	A	C	18: 78,393,305 (GRCm39)	S100R	probably benign	Het
Iba57	T	C	11: 59,054,265 (GRCm39)	T85A	probably benign	Het
Ipo4	A	T	14: 55,872,983 (GRCm39)	M1K	probably null	Het
Ippk	A	G	13: 49,584,341 (GRCm39)		probably null	Het
Kcnk10	A	T	12: 98,456,204 (GRCm39)	I209N	probably benign	Het
Klk14	T	C	7: 43,343,534 (GRCm39)	L122P	probably benign	Het
Ltbp1	T	A	17: 75,589,424 (GRCm39)		probably null	Het
Mab21l4	A	T	1: 93,080,766 (GRCm39)	I380N	probably damaging	Het
Mcm3	T	C	1: 20,875,071 (GRCm39)	D737G	probably benign	Het
Naip2	A	C	13: 100,298,290 (GRCm39)	I582S	probably benign	Het
Nalcn	T	A	14: 123,528,459 (GRCm39)	T1456S	probably benign	Het
Nckap1l	C	T	15: 103,405,854 (GRCm39)	P1097S	probably benign	Het
Ncoa1	A	G	12: 4,317,687 (GRCm39)	M1215T	possibly damaging	Het
Obox5	T	A	7: 15,491,932 (GRCm39)	C116S	possibly damaging	Het
Or2ag2b	T	A	7: 106,417,568 (GRCm39)	S93T	possibly damaging	Het
Or51a43	C	T	7: 103,717,332 (GRCm39)	R302H	probably benign	Het
Or5a1	C	A	19: 12,097,900 (GRCm39)	A59S	probably benign	Het
Pcdhb14	G	T	18: 37,582,277 (GRCm39)	R461L	probably damaging	Het
Pdgfra	A	G	5: 75,355,697 (GRCm39)	D1069G	probably damaging	Het
Pgr	C	T	9: 8,900,779 (GRCm39)	A104V	possibly damaging	Het
Pkd1l3	C	G	8: 110,350,281 (GRCm39)	D375E	possibly damaging	Het
Potegl	A	G	2: 23,102,832 (GRCm39)	E190G	possibly damaging	Het
Rassf3	A	G	10: 121,253,109 (GRCm39)		probably benign	Het
Rgs22	G	T	15: 36,099,941 (GRCm39)	S258*	probably null	Het
Rsph6a	C	A	7: 18,791,594 (GRCm39)	D254E	possibly damaging	Het
Sgk1	A	G	10: 21,872,257 (GRCm39)		probably benign	Het
Shcbp1l	G	A	1: 153,308,928 (GRCm39)	C174Y	probably damaging	Het
Spata31g1	T	C	4: 42,972,715 (GRCm39)	S683P	probably benign	Het
Sulf1	T	A	1: 12,867,144 (GRCm39)	N109K	probably damaging	Het
Taf7l2	G	A	10: 115,949,058 (GRCm39)	A156V	probably benign	Het
Tas2r115	T	A	6: 132,714,682 (GRCm39)	I90L	probably benign	Het
Tmem200a	T	C	10: 25,870,002 (GRCm39)	H89R	probably benign	Het
Ubxn4	G	A	1: 128,190,641 (GRCm39)	E256K	probably benign	Het
Xrn1	T	C	9: 95,906,244 (GRCm39)	S1212P	probably damaging	Het
Zfp408	T	C	2: 91,475,882 (GRCm39)	Y424C	possibly damaging	Het

Other mutations in Nomo1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00331:Nomo1	APN	7	45,694,760 (GRCm39)	missense	possibly damaging	0.66
IGL00811:Nomo1	APN	7	45,732,732 (GRCm39)	missense	possibly damaging	0.95
IGL01710:Nomo1	APN	7	45,687,980 (GRCm39)	missense	probably damaging	1.00
IGL01797:Nomo1	APN	7	45,706,086 (GRCm39)	missense	probably damaging	0.96
IGL01973:Nomo1	APN	7	45,732,651 (GRCm39)	splice site	probably benign
IGL02506:Nomo1	APN	7	45,727,480 (GRCm39)	missense	possibly damaging	0.50
IGL02739:Nomo1	APN	7	45,693,731 (GRCm39)	splice site	probably null
IGL02863:Nomo1	APN	7	45,696,340 (GRCm39)	missense	probably damaging	0.98
P0005:Nomo1	UTSW	7	45,686,981 (GRCm39)	critical splice acceptor site	probably null
PIT4243001:Nomo1	UTSW	7	45,693,705 (GRCm39)	missense	probably damaging	1.00
R0106:Nomo1	UTSW	7	45,687,056 (GRCm39)	missense	probably damaging	1.00
R0106:Nomo1	UTSW	7	45,687,056 (GRCm39)	missense	probably damaging	1.00
R0124:Nomo1	UTSW	7	45,732,652 (GRCm39)	splice site	probably benign
R0239:Nomo1	UTSW	7	45,729,018 (GRCm39)	critical splice donor site	probably null
R0239:Nomo1	UTSW	7	45,729,018 (GRCm39)	critical splice donor site	probably null
R0417:Nomo1	UTSW	7	45,718,122 (GRCm39)	missense	possibly damaging	0.51
R0535:Nomo1	UTSW	7	45,721,941 (GRCm39)	missense	probably damaging	0.99
R0829:Nomo1	UTSW	7	45,725,596 (GRCm39)	splice site	probably benign
R0940:Nomo1	UTSW	7	45,683,329 (GRCm39)	missense	possibly damaging	0.56
R1480:Nomo1	UTSW	7	45,710,337 (GRCm39)	missense	probably damaging	0.98
R1601:Nomo1	UTSW	7	45,696,379 (GRCm39)	missense	probably damaging	0.96
R1743:Nomo1	UTSW	7	45,719,461 (GRCm39)	critical splice donor site	probably null
R1765:Nomo1	UTSW	7	45,715,717 (GRCm39)	missense	possibly damaging	0.59
R1861:Nomo1	UTSW	7	45,727,525 (GRCm39)	missense	probably benign	0.06
R1998:Nomo1	UTSW	7	45,683,368 (GRCm39)	missense	possibly damaging	0.69
R1999:Nomo1	UTSW	7	45,706,151 (GRCm39)	missense	possibly damaging	0.95
R2145:Nomo1	UTSW	7	45,715,928 (GRCm39)	missense	probably damaging	1.00
R2869:Nomo1	UTSW	7	45,696,361 (GRCm39)	missense	probably damaging	0.96
R2869:Nomo1	UTSW	7	45,696,361 (GRCm39)	missense	probably damaging	0.96
R2870:Nomo1	UTSW	7	45,696,361 (GRCm39)	missense	probably damaging	0.96
R2870:Nomo1	UTSW	7	45,696,361 (GRCm39)	missense	probably damaging	0.96
R2871:Nomo1	UTSW	7	45,696,361 (GRCm39)	missense	probably damaging	0.96
R2871:Nomo1	UTSW	7	45,696,361 (GRCm39)	missense	probably damaging	0.96
R2873:Nomo1	UTSW	7	45,696,361 (GRCm39)	missense	probably damaging	0.96
R4116:Nomo1	UTSW	7	45,683,320 (GRCm39)	missense	probably benign	0.06
R4404:Nomo1	UTSW	7	45,706,092 (GRCm39)	missense	probably benign	0.00
R4406:Nomo1	UTSW	7	45,706,092 (GRCm39)	missense	probably benign	0.00
R4560:Nomo1	UTSW	7	45,690,904 (GRCm39)	missense	probably damaging	0.99
R4633:Nomo1	UTSW	7	45,699,684 (GRCm39)	splice site	probably benign
R4651:Nomo1	UTSW	7	45,717,866 (GRCm39)	missense	probably damaging	0.99
R4653:Nomo1	UTSW	7	45,711,237 (GRCm39)	missense	probably benign	0.01
R4752:Nomo1	UTSW	7	45,706,626 (GRCm39)	missense	probably damaging	1.00
R4792:Nomo1	UTSW	7	45,693,643 (GRCm39)	splice site	probably null
R4838:Nomo1	UTSW	7	45,733,139 (GRCm39)	missense	unknown
R4876:Nomo1	UTSW	7	45,715,915 (GRCm39)	missense	probably damaging	1.00
R4915:Nomo1	UTSW	7	45,693,656 (GRCm39)	missense	probably benign	0.30
R4953:Nomo1	UTSW	7	45,700,155 (GRCm39)	intron	probably benign
R5463:Nomo1	UTSW	7	45,712,426 (GRCm39)	missense	possibly damaging	0.47
R5664:Nomo1	UTSW	7	45,725,581 (GRCm39)	missense	probably benign
R5956:Nomo1	UTSW	7	45,692,037 (GRCm39)	missense	possibly damaging	0.51
R6037:Nomo1	UTSW	7	45,712,423 (GRCm39)	missense	possibly damaging	0.64
R6037:Nomo1	UTSW	7	45,712,423 (GRCm39)	missense	possibly damaging	0.64
R6307:Nomo1	UTSW	7	45,683,260 (GRCm39)	unclassified	probably benign
R6695:Nomo1	UTSW	7	45,715,885 (GRCm39)	missense	probably benign	0.16
R6970:Nomo1	UTSW	7	45,695,391 (GRCm39)	missense	probably damaging	0.97
R7334:Nomo1	UTSW	7	45,732,692 (GRCm39)	missense	probably damaging	1.00
R7394:Nomo1	UTSW	7	45,715,903 (GRCm39)	missense	probably benign	0.26
R7556:Nomo1	UTSW	7	45,715,642 (GRCm39)	missense	probably damaging	1.00
R7834:Nomo1	UTSW	7	45,706,162 (GRCm39)	critical splice donor site	probably null
R7979:Nomo1	UTSW	7	45,690,986 (GRCm39)	missense	probably null
R8193:Nomo1	UTSW	7	45,692,037 (GRCm39)	missense	possibly damaging	0.51
R8841:Nomo1	UTSW	7	45,707,911 (GRCm39)	missense	probably benign	0.00
R8906:Nomo1	UTSW	7	45,722,004 (GRCm39)	missense	probably benign	0.06
R9049:Nomo1	UTSW	7	45,715,597 (GRCm39)	missense	probably benign	0.01
R9087:Nomo1	UTSW	7	45,732,748 (GRCm39)	missense	probably benign	0.00
R9176:Nomo1	UTSW	7	45,730,828 (GRCm39)	missense	possibly damaging	0.88
Z1177:Nomo1	UTSW	7	45,715,697 (GRCm39)	missense	probably benign	0.17

Predicted Primers

PCR Primer
(F):5'- ACAGACAGATAGCCTTTCTGTTCTTGC -3'
(R):5'- CCCACAGAACAAGACAGGCATGTTG -3'

Sequencing Primer
(F):5'- CACTTTGGATGAAGGATAATCAGCTC -3'
(R):5'- TGTCGGCCAAAATACGAGC -3'

Posted On

2013-05-23