Mutagenetix > Incidental Mutation

Incidental Mutation 'R0467:Rassf3'

41701

Institutional Source

Beutler Lab

Gene Symbol

Rassf3

Ensembl Gene

ENSMUSG00000025795

Gene Name

Ras association (RalGDS/AF-6) domain family member 3

Synonyms

MMRRC Submission

038667-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.114) question?

Stock #

R0467 (G1)

Quality Score

Status

Validated

Chromosome

Chromosomal Location

121246255-121312220 bp(-) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

A to G at 121253109 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000026902 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000026902]

AlphaFold

Q99P51

Predicted Effect

probably benign
Transcript: ENSMUST00000026902

SMART Domains

Protein: ENSMUSP00000026902
Gene: ENSMUSG00000025795

Domain	Start	End	E-Value	Type
low complexity region	14	26	N/A	INTRINSIC
RA	78	181	1.98e-3	SMART
Pfam:Nore1-SARAH	186	225	1e-20	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000219500

Coding Region Coverage

1x: 99.6%
3x: 98.8%
10x: 96.7%
20x: 93.3%

Validation Efficiency

98% (52/53)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The RAS oncogene (MIM 190020) is mutated in nearly one-third of all human cancers. Members of the RAS superfamily are plasma membrane GTP-binding proteins that modulate intracellular signal transduction pathways. A subfamily of RAS effectors, including RASSF3, share a RAS association (RA) domain.[supplied by OMIM, Jul 2003]

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca17	A	G	17: 24,532,151 (GRCm39)		probably benign	Het
Anapc1	A	G	2: 128,510,963 (GRCm39)	I511T	probably damaging	Het
Atf6	A	T	1: 170,621,589 (GRCm39)	H477Q	probably damaging	Het
C4b	A	G	17: 34,955,101 (GRCm39)	V795A	probably benign	Het
Cdh26	C	T	2: 178,123,425 (GRCm39)	R675C	possibly damaging	Het
Cdk12	T	C	11: 98,094,405 (GRCm39)	V71A	probably damaging	Het
Cul3	A	T	1: 80,258,580 (GRCm39)	D419E	probably benign	Het
Ddi2	A	G	4: 141,412,495 (GRCm39)	I139T	probably benign	Het
Dnaaf1	T	A	8: 120,317,471 (GRCm39)	D333E	probably benign	Het
Dnase1	A	G	16: 3,857,013 (GRCm39)	D7G	probably damaging	Het
G3bp1	T	C	11: 55,389,452 (GRCm39)	F383L	probably damaging	Het
Galc	A	C	12: 98,208,904 (GRCm39)	I250R	probably damaging	Het
Garin1b	G	A	6: 29,326,606 (GRCm39)	S241N	probably damaging	Het
Gcfc2	T	C	6: 81,900,863 (GRCm39)	V59A	possibly damaging	Het
Gm6133	A	C	18: 78,393,305 (GRCm39)	S100R	probably benign	Het
Iba57	T	C	11: 59,054,265 (GRCm39)	T85A	probably benign	Het
Ipo4	A	T	14: 55,872,983 (GRCm39)	M1K	probably null	Het
Ippk	A	G	13: 49,584,341 (GRCm39)		probably null	Het
Kcnk10	A	T	12: 98,456,204 (GRCm39)	I209N	probably benign	Het
Klk14	T	C	7: 43,343,534 (GRCm39)	L122P	probably benign	Het
Ltbp1	T	A	17: 75,589,424 (GRCm39)		probably null	Het
Mab21l4	A	T	1: 93,080,766 (GRCm39)	I380N	probably damaging	Het
Mcm3	T	C	1: 20,875,071 (GRCm39)	D737G	probably benign	Het
Naip2	A	C	13: 100,298,290 (GRCm39)	I582S	probably benign	Het
Nalcn	T	A	14: 123,528,459 (GRCm39)	T1456S	probably benign	Het
Nckap1l	C	T	15: 103,405,854 (GRCm39)	P1097S	probably benign	Het
Ncoa1	A	G	12: 4,317,687 (GRCm39)	M1215T	possibly damaging	Het
Nomo1	T	A	7: 45,721,911 (GRCm39)		probably null	Het
Obox5	T	A	7: 15,491,932 (GRCm39)	C116S	possibly damaging	Het
Or2ag2b	T	A	7: 106,417,568 (GRCm39)	S93T	possibly damaging	Het
Or51a43	C	T	7: 103,717,332 (GRCm39)	R302H	probably benign	Het
Or5a1	C	A	19: 12,097,900 (GRCm39)	A59S	probably benign	Het
Pcdhb14	G	T	18: 37,582,277 (GRCm39)	R461L	probably damaging	Het
Pdgfra	A	G	5: 75,355,697 (GRCm39)	D1069G	probably damaging	Het
Pgr	C	T	9: 8,900,779 (GRCm39)	A104V	possibly damaging	Het
Pkd1l3	C	G	8: 110,350,281 (GRCm39)	D375E	possibly damaging	Het
Potegl	A	G	2: 23,102,832 (GRCm39)	E190G	possibly damaging	Het
Rgs22	G	T	15: 36,099,941 (GRCm39)	S258*	probably null	Het
Rsph6a	C	A	7: 18,791,594 (GRCm39)	D254E	possibly damaging	Het
Sgk1	A	G	10: 21,872,257 (GRCm39)		probably benign	Het
Shcbp1l	G	A	1: 153,308,928 (GRCm39)	C174Y	probably damaging	Het
Spata31g1	T	C	4: 42,972,715 (GRCm39)	S683P	probably benign	Het
Sulf1	T	A	1: 12,867,144 (GRCm39)	N109K	probably damaging	Het
Taf7l2	G	A	10: 115,949,058 (GRCm39)	A156V	probably benign	Het
Tas2r115	T	A	6: 132,714,682 (GRCm39)	I90L	probably benign	Het
Tmem200a	T	C	10: 25,870,002 (GRCm39)	H89R	probably benign	Het
Ubxn4	G	A	1: 128,190,641 (GRCm39)	E256K	probably benign	Het
Xrn1	T	C	9: 95,906,244 (GRCm39)	S1212P	probably damaging	Het
Zfp408	T	C	2: 91,475,882 (GRCm39)	Y424C	possibly damaging	Het

Other mutations in Rassf3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00783:Rassf3	APN	10	121,251,985 (GRCm39)	missense	probably benign	0.00
IGL01955:Rassf3	APN	10	121,253,027 (GRCm39)	missense	probably damaging	1.00
BB005:Rassf3	UTSW	10	121,252,984 (GRCm39)	splice site	probably null
BB015:Rassf3	UTSW	10	121,252,984 (GRCm39)	splice site	probably null
R0418:Rassf3	UTSW	10	121,253,075 (GRCm39)	missense	probably benign	0.42
R1167:Rassf3	UTSW	10	121,252,159 (GRCm39)	missense	probably damaging	1.00
R2906:Rassf3	UTSW	10	121,250,297 (GRCm39)	missense	probably damaging	1.00
R3801:Rassf3	UTSW	10	121,250,271 (GRCm39)	missense	possibly damaging	0.76
R7146:Rassf3	UTSW	10	121,252,052 (GRCm39)	missense	probably benign	0.06
R7257:Rassf3	UTSW	10	121,248,924 (GRCm39)	nonsense	probably null
R7579:Rassf3	UTSW	10	121,312,103 (GRCm39)	start gained	probably benign
R7928:Rassf3	UTSW	10	121,252,984 (GRCm39)	splice site	probably null
R8783:Rassf3	UTSW	10	121,253,069 (GRCm39)	missense	probably benign
R9010:Rassf3	UTSW	10	121,311,991 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- AAGCCTGGAGTGTATCTCAGCTCG -3'
(R):5'- CAGATGACCCTGGTGTTCTTGATGG -3'

Sequencing Primer
(F):5'- GGATCAAACCTGCCAGCTTG -3'
(R):5'- GTAAACTAGAGCTGCCTGTGTC -3'

Posted On

2013-05-23