Mutagenetix > Incidental Mutations

Incidental Mutation 'R0477:Mdn1'

41843

Institutional Source

Beutler Lab

Gene Symbol

Mdn1

Ensembl Gene

ENSMUSG00000058006

Gene Name

midasin AAA ATPase 1

Synonyms

4833432B22Rik, LOC213784, D4Abb1e

MMRRC Submission

038677-MU

Accession Numbers

Genbank: NM_001081392; MGI: 1926159

Is this an essential gene?

Essential (E-score: 1.000) question?

Stock #

R0477 (G1)

Quality Score

225

Status

Validated (trace)

Chromosome

Chromosomal Location

32657119-32775217 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to C at 32750928 bp

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Alanine at position 4487 (E4487A)

Ref Sequence

ENSEMBL: ENSMUSP00000136222 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000071642] [ENSMUST00000178134]

Predicted Effect

probably benign
Transcript: ENSMUST00000071642
AA Change: E4487A

PolyPhen 2 Score 0.021 (Sensitivity: 0.95; Specificity: 0.80)

SMART Domains

Protein: ENSMUSP00000071569
Gene: ENSMUSG00000058006
AA Change: E4487A

Domain	Start	End	E-Value	Type
low complexity region	48	75	N/A	INTRINSIC
low complexity region	258	270	N/A	INTRINSIC
Pfam:AAA_5	324	459	7.4e-19	PFAM
AAA	666	908	1.06e-6	SMART
AAA	1072	1217	2.09e-1	SMART
AAA	1378	1544	8.27e-9	SMART
AAA	1740	1893	6.78e-2	SMART
AAA	2053	2309	1.62e0	SMART
low complexity region	3181	3193	N/A	INTRINSIC
low complexity region	3541	3552	N/A	INTRINSIC
low complexity region	3557	3565	N/A	INTRINSIC
low complexity region	4189	4203	N/A	INTRINSIC
low complexity region	4339	4353	N/A	INTRINSIC
low complexity region	4672	4681	N/A	INTRINSIC
low complexity region	4735	4756	N/A	INTRINSIC
low complexity region	4769	4790	N/A	INTRINSIC
low complexity region	4886	4905	N/A	INTRINSIC
low complexity region	4924	4937	N/A	INTRINSIC
coiled coil region	4957	4983	N/A	INTRINSIC
low complexity region	5000	5017	N/A	INTRINSIC
low complexity region	5176	5192	N/A	INTRINSIC
low complexity region	5315	5329	N/A	INTRINSIC
VWA	5375	5556	2.73e-6	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000125323

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000138577

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000149941

Predicted Effect

probably benign
Transcript: ENSMUST00000178134
AA Change: E4487A

PolyPhen 2 Score 0.021 (Sensitivity: 0.95; Specificity: 0.80)

SMART Domains

Protein: ENSMUSP00000136222
Gene: ENSMUSG00000058006
AA Change: E4487A

Domain	Start	End	E-Value	Type
low complexity region	48	75	N/A	INTRINSIC
low complexity region	258	270	N/A	INTRINSIC
Pfam:AAA_5	324	459	4.4e-19	PFAM
AAA	666	908	1.06e-6	SMART
AAA	1072	1217	2.09e-1	SMART
AAA	1378	1544	8.27e-9	SMART
AAA	1740	1893	6.78e-2	SMART
AAA	2053	2309	1.62e0	SMART
low complexity region	3181	3193	N/A	INTRINSIC
low complexity region	3541	3552	N/A	INTRINSIC
low complexity region	3557	3565	N/A	INTRINSIC
low complexity region	4189	4203	N/A	INTRINSIC
low complexity region	4339	4353	N/A	INTRINSIC
low complexity region	4667	4676	N/A	INTRINSIC
low complexity region	4730	4751	N/A	INTRINSIC
low complexity region	4764	4785	N/A	INTRINSIC
low complexity region	4881	4900	N/A	INTRINSIC
low complexity region	4919	4932	N/A	INTRINSIC
coiled coil region	4952	4978	N/A	INTRINSIC
low complexity region	4992	5010	N/A	INTRINSIC
low complexity region	5169	5185	N/A	INTRINSIC
low complexity region	5308	5322	N/A	INTRINSIC
VWA	5368	5549	2.73e-6	SMART

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.4%
10x: 96.4%
20x: 93.1%

Validation Efficiency

98% (57/58)

Allele List at MGI

All alleles(29) : Targeted, other(2) Gene trapped(27)

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
6030468B19Rik	A	T	11: 117,802,961	I85F	probably benign	Het
Abca8a	T	A	11: 110,065,225	I778L	probably benign	Het
Abcc5	T	C	16: 20,368,569	N889S	possibly damaging	Het
Abcc5	T	C	16: 20,398,885	N359D	probably damaging	Het
Adam23	A	G	1: 63,557,400		probably benign	Het
Adamts3	A	T	5: 89,684,507	D913E	probably benign	Het
Ap1b1	G	T	11: 5,031,787	C538F	probably benign	Het
Ash1l	T	A	3: 88,983,459	S882T	probably benign	Het
C9	A	T	15: 6,458,183	E43D	probably benign	Het
Cacna2d1	T	C	5: 16,194,798		probably null	Het
Ces2a	A	G	8: 104,737,537	E267G	probably damaging	Het
Cfap61	A	G	2: 145,939,916	D23G	probably damaging	Het
Col9a3	T	G	2: 180,609,470		probably benign	Het
Cstl1	T	C	2: 148,750,988	V21A	probably benign	Het
Cth	A	T	3: 157,905,175	L340Q	probably damaging	Het
Dnah8	T	A	17: 30,755,080	M2813K	probably damaging	Het
Fam107a	A	T	14: 8,301,168	Y21N	probably benign	Het
Fam184a	G	A	10: 53,655,079	T733M	probably damaging	Het
Fer1l4	A	G	2: 156,052,886	V21A	probably benign	Het
Foxc2	A	T	8: 121,118,035	Y474F	probably damaging	Het
Hnf4g	G	T	3: 3,651,791		probably benign	Het
Hnrnpll	T	C	17: 80,061,832	D54G	unknown	Het
Hydin	A	G	8: 110,418,498	Y827C	probably damaging	Het
Il23r	A	G	6: 67,452,377	V327A	probably benign	Het
Itih4	T	A	14: 30,889,674	V118D	probably damaging	Het
Kmt2d	G	A	15: 98,853,581		probably benign	Het
Lamb1	A	G	12: 31,326,269	D1546G	possibly damaging	Het
Large1	A	T	8: 72,818,082	D689E	probably damaging	Het
Map1a	T	C	2: 121,302,101	S895P	probably damaging	Het
Myo15	T	C	11: 60,520,914		probably null	Het
Nlrp4f	C	A	13: 65,190,906	R639L	probably benign	Het
Olfr1100	T	A	2: 86,978,223	D191V	probably damaging	Het
Olfr1275	A	G	2: 111,231,664	F43S	probably benign	Het
Pcdh9	T	C	14: 93,887,678	N229S	probably damaging	Het
Pcnx2	A	G	8: 125,761,567	V1746A	probably damaging	Het
Phf12	A	T	11: 78,023,070	H446L	possibly damaging	Het
Phlpp2	A	G	8: 109,895,506		probably null	Het
Psmb9	A	C	17: 34,182,264	V207G	probably damaging	Het
Ptprh	C	A	7: 4,597,998	D127Y	possibly damaging	Het
Rabep1	T	G	11: 70,920,907	M535R	probably damaging	Het
Rif1	GCCACCA	GCCA	2: 52,110,324		probably benign	Het
Scin	T	C	12: 40,060,516	D711G	probably damaging	Het
Slfn4	T	C	11: 83,188,681	I6T	probably benign	Het
Sos1	T	A	17: 80,434,934	E388V	possibly damaging	Het
Spag5	A	C	11: 78,314,198	Q603P	probably damaging	Het
Supv3l1	G	T	10: 62,430,585	T604N	probably damaging	Het
Tbx5	A	G	5: 119,883,119	S397G	possibly damaging	Het
Tmprss5	A	G	9: 49,115,165	D383G	possibly damaging	Het
Trim43b	A	G	9: 89,090,601	W167R	probably damaging	Het
Unc80	A	T	1: 66,570,001	D1283V	probably damaging	Het
Upf1	A	T	8: 70,334,080	V918D	probably benign	Het
Vmn2r100	A	G	17: 19,522,514	I383M	probably benign	Het
Zc3h3	G	T	15: 75,777,083	S733R	possibly damaging	Het
Zcchc2	C	T	1: 106,030,270	P426S	possibly damaging	Het
Zkscan7	A	G	9: 122,890,809		probably null	Het

Other mutations in Mdn1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00088:Mdn1	APN	4	32723651	missense	probably damaging	1.00
IGL00426:Mdn1	APN	4	32719214	missense	possibly damaging	0.91
IGL00570:Mdn1	APN	4	32735719	missense	probably benign
IGL00573:Mdn1	APN	4	32666619	critical splice donor site	probably null
IGL00983:Mdn1	APN	4	32735525	missense	probably damaging	1.00
IGL01288:Mdn1	APN	4	32730864	missense	probably benign	0.00
IGL01359:Mdn1	APN	4	32743686	missense	probably benign	0.10
IGL01457:Mdn1	APN	4	32715922	missense	possibly damaging	0.82
IGL01530:Mdn1	APN	4	32711938	splice site	probably benign
IGL01684:Mdn1	APN	4	32726857	missense	probably benign
IGL01753:Mdn1	APN	4	32708483	missense	probably benign
IGL01901:Mdn1	APN	4	32669591	missense	probably damaging	1.00
IGL01952:Mdn1	APN	4	32723657	missense	possibly damaging	0.82
IGL01960:Mdn1	APN	4	32758393	missense	probably benign	0.14
IGL02019:Mdn1	APN	4	32749948	missense	possibly damaging	0.93
IGL02100:Mdn1	APN	4	32715708	missense	possibly damaging	0.90
IGL02117:Mdn1	APN	4	32709364	missense	probably benign	0.00
IGL02154:Mdn1	APN	4	32740395	missense	probably benign	0.35
IGL02216:Mdn1	APN	4	32739092	missense	probably benign	0.03
IGL02371:Mdn1	APN	4	32676860	splice site	probably benign
IGL02396:Mdn1	APN	4	32700120	missense	probably damaging	0.99
IGL02454:Mdn1	APN	4	32694674	critical splice donor site	probably null
IGL02502:Mdn1	APN	4	32670579	missense	possibly damaging	0.69
IGL02883:Mdn1	APN	4	32763199	missense	probably benign	0.05
IGL02946:Mdn1	APN	4	32734366	missense	probably damaging	0.98
IGL02950:Mdn1	APN	4	32713360	splice site	probably benign
IGL03076:Mdn1	APN	4	32735564	missense	probably damaging	0.97
IGL03129:Mdn1	APN	4	32729994	missense	possibly damaging	0.47
IGL03234:Mdn1	APN	4	32732842	missense	probably benign	0.06
3-1:Mdn1	UTSW	4	32725967	critical splice donor site	probably null
IGL03046:Mdn1	UTSW	4	32694495	missense	possibly damaging	0.73
P0035:Mdn1	UTSW	4	32749934	missense	probably benign	0.05
PIT4508001:Mdn1	UTSW	4	32719223	missense	probably damaging	0.97
PIT4618001:Mdn1	UTSW	4	32746527	missense	probably benign	0.20
R0008:Mdn1	UTSW	4	32718317	missense	possibly damaging	0.47
R0110:Mdn1	UTSW	4	32738619	missense	probably benign	0.20
R0125:Mdn1	UTSW	4	32729956	missense	probably damaging	0.98
R0257:Mdn1	UTSW	4	32693534	missense	probably damaging	0.99
R0266:Mdn1	UTSW	4	32741835	missense	probably damaging	0.99
R0349:Mdn1	UTSW	4	32750318	missense	probably damaging	1.00
R0362:Mdn1	UTSW	4	32746439	critical splice acceptor site	probably null
R0421:Mdn1	UTSW	4	32684707	missense	probably benign	0.39
R0450:Mdn1	UTSW	4	32738619	missense	probably benign	0.20
R0465:Mdn1	UTSW	4	32699204	splice site	probably benign
R0469:Mdn1	UTSW	4	32738619	missense	probably benign	0.20
R0481:Mdn1	UTSW	4	32767182	splice site	probably benign
R0504:Mdn1	UTSW	4	32698916	splice site	probably benign
R0522:Mdn1	UTSW	4	32672837	missense	probably benign	0.09
R0550:Mdn1	UTSW	4	32730479	missense	probably benign	0.13
R0607:Mdn1	UTSW	4	32712014	missense	probably damaging	1.00
R0607:Mdn1	UTSW	4	32732829	missense	probably benign	0.36
R0664:Mdn1	UTSW	4	32768011	nonsense	probably null
R0701:Mdn1	UTSW	4	32699263	missense	probably benign	0.00
R0801:Mdn1	UTSW	4	32668895	missense	probably benign	0.04
R0841:Mdn1	UTSW	4	32752032	missense	probably benign	0.23
R0849:Mdn1	UTSW	4	32741835	missense	probably damaging	0.99
R0893:Mdn1	UTSW	4	32701713	missense	probably benign	0.01
R1114:Mdn1	UTSW	4	32746568	critical splice donor site	probably null
R1137:Mdn1	UTSW	4	32694511	missense	probably damaging	1.00
R1185:Mdn1	UTSW	4	32735576	missense	possibly damaging	0.94
R1185:Mdn1	UTSW	4	32735576	missense	possibly damaging	0.94
R1185:Mdn1	UTSW	4	32735576	missense	possibly damaging	0.94
R1257:Mdn1	UTSW	4	32667089	critical splice acceptor site	probably null
R1356:Mdn1	UTSW	4	32700334	splice site	probably benign
R1466:Mdn1	UTSW	4	32730788	missense	probably benign	0.28
R1466:Mdn1	UTSW	4	32730788	missense	probably benign	0.28
R1518:Mdn1	UTSW	4	32739977	missense	probably damaging	1.00
R1569:Mdn1	UTSW	4	32723501	missense	probably null	0.10
R1574:Mdn1	UTSW	4	32722315	missense	probably benign
R1574:Mdn1	UTSW	4	32722315	missense	probably benign
R1591:Mdn1	UTSW	4	32700092	missense	possibly damaging	0.65
R1678:Mdn1	UTSW	4	32663050	missense	probably damaging	0.99
R1696:Mdn1	UTSW	4	32700417	missense	possibly damaging	0.91
R1707:Mdn1	UTSW	4	32693504	missense	probably damaging	1.00
R1749:Mdn1	UTSW	4	32773952	missense	probably damaging	1.00
R1780:Mdn1	UTSW	4	32700103	missense	probably damaging	1.00
R1833:Mdn1	UTSW	4	32720761	missense	probably damaging	0.97
R1858:Mdn1	UTSW	4	32730881	missense	probably benign	0.17
R1870:Mdn1	UTSW	4	32763339	missense	probably damaging	1.00
R1887:Mdn1	UTSW	4	32742540	missense	probably damaging	1.00
R1909:Mdn1	UTSW	4	32760839	small deletion	probably benign
R2075:Mdn1	UTSW	4	32716058	missense	probably benign	0.03
R2103:Mdn1	UTSW	4	32738712	missense	possibly damaging	0.75
R2104:Mdn1	UTSW	4	32743843	splice site	probably null
R2110:Mdn1	UTSW	4	32700409	missense	probably damaging	1.00
R2111:Mdn1	UTSW	4	32700409	missense	probably damaging	1.00
R2206:Mdn1	UTSW	4	32716271	missense	possibly damaging	0.71
R2221:Mdn1	UTSW	4	32763306	missense	probably benign	0.37
R2240:Mdn1	UTSW	4	32765701	missense	possibly damaging	0.90
R2351:Mdn1	UTSW	4	32750010	missense	probably benign	0.21
R2421:Mdn1	UTSW	4	32723621	missense	probably damaging	0.96
R3036:Mdn1	UTSW	4	32750013	missense	probably damaging	0.99
R3434:Mdn1	UTSW	4	32733726	critical splice donor site	probably null
R3435:Mdn1	UTSW	4	32733726	critical splice donor site	probably null
R3783:Mdn1	UTSW	4	32720818	missense	probably benign	0.01
R3811:Mdn1	UTSW	4	32693506	nonsense	probably null
R3973:Mdn1	UTSW	4	32722363	missense	probably benign	0.00
R4154:Mdn1	UTSW	4	32707475	missense	probably damaging	0.96
R4372:Mdn1	UTSW	4	32743809	missense	probably benign	0.03
R4393:Mdn1	UTSW	4	32754482	missense	possibly damaging	0.48
R4438:Mdn1	UTSW	4	32704635	missense	probably damaging	1.00
R4471:Mdn1	UTSW	4	32668860	missense	probably benign	0.00
R4509:Mdn1	UTSW	4	32715883	missense	probably damaging	1.00
R4538:Mdn1	UTSW	4	32722334	missense	probably damaging	1.00
R4557:Mdn1	UTSW	4	32754437	missense	probably damaging	1.00
R4570:Mdn1	UTSW	4	32741812	missense	probably damaging	1.00
R4591:Mdn1	UTSW	4	32707636	missense	probably damaging	1.00
R4658:Mdn1	UTSW	4	32730749	splice site	probably null
R4667:Mdn1	UTSW	4	32679572	missense	probably damaging	1.00
R4684:Mdn1	UTSW	4	32666430	missense	probably damaging	1.00
R4778:Mdn1	UTSW	4	32683583	nonsense	probably null
R4807:Mdn1	UTSW	4	32685651	splice site	probably null
R4923:Mdn1	UTSW	4	32671608	missense	possibly damaging	0.89
R4951:Mdn1	UTSW	4	32707459	missense	probably damaging	1.00
R4963:Mdn1	UTSW	4	32756512	missense	probably benign	0.00
R4971:Mdn1	UTSW	4	32739827	missense	probably damaging	1.00
R4973:Mdn1	UTSW	4	32734418	missense	probably benign	0.01
R5122:Mdn1	UTSW	4	32670593	missense	probably damaging	1.00
R5159:Mdn1	UTSW	4	32774008	missense	possibly damaging	0.93
R5164:Mdn1	UTSW	4	32759011	intron	probably null
R5215:Mdn1	UTSW	4	32741418	missense	possibly damaging	0.78
R5217:Mdn1	UTSW	4	32723690	missense	probably damaging	0.98
R5219:Mdn1	UTSW	4	32723690	missense	probably damaging	0.98
R5365:Mdn1	UTSW	4	32723690	missense	probably damaging	0.98
R5366:Mdn1	UTSW	4	32723690	missense	probably damaging	0.98
R5368:Mdn1	UTSW	4	32723690	missense	probably damaging	0.98
R5445:Mdn1	UTSW	4	32723690	missense	probably damaging	0.98
R5462:Mdn1	UTSW	4	32720897	missense	probably benign
R5522:Mdn1	UTSW	4	32685783	missense	probably damaging	1.00
R5525:Mdn1	UTSW	4	32767961	missense	possibly damaging	0.73
R5578:Mdn1	UTSW	4	32728167	missense	probably benign	0.04
R5605:Mdn1	UTSW	4	32765664	missense	probably benign
R5621:Mdn1	UTSW	4	32716371	missense	possibly damaging	0.46
R5636:Mdn1	UTSW	4	32695480	missense	probably damaging	1.00
R5650:Mdn1	UTSW	4	32667467	splice site	probably null
R5780:Mdn1	UTSW	4	32722950	missense	probably benign	0.02
R5838:Mdn1	UTSW	4	32754547	missense	probably damaging	0.99
R5857:Mdn1	UTSW	4	32670646	missense	probably benign	0.09
R5895:Mdn1	UTSW	4	32695400	missense	probably damaging	1.00
R5943:Mdn1	UTSW	4	32678330	missense	probably damaging	1.00
R6008:Mdn1	UTSW	4	32741073	missense	probably damaging	1.00
R6013:Mdn1	UTSW	4	32715713	missense	probably damaging	1.00
R6075:Mdn1	UTSW	4	32689581	missense	possibly damaging	0.48
R6151:Mdn1	UTSW	4	32684735	missense	probably damaging	1.00
R6163:Mdn1	UTSW	4	32716040	missense	probably damaging	1.00
R6181:Mdn1	UTSW	4	32715953	missense	probably damaging	1.00
R6211:Mdn1	UTSW	4	32696269	missense	probably benign	0.12
R6249:Mdn1	UTSW	4	32708484	missense	possibly damaging	0.85
R6251:Mdn1	UTSW	4	32748590	missense	probably benign	0.13
R6253:Mdn1	UTSW	4	32749593	missense	probably benign	0.25
R6273:Mdn1	UTSW	4	32715979	missense	probably benign	0.01
R6297:Mdn1	UTSW	4	32730054	nonsense	probably null
R6384:Mdn1	UTSW	4	32670607	missense	probably damaging	1.00
R6463:Mdn1	UTSW	4	32773308	missense	probably damaging	1.00
R6528:Mdn1	UTSW	4	32713780	missense	probably damaging	1.00
R6688:Mdn1	UTSW	4	32774041	missense	possibly damaging	0.74
R6762:Mdn1	UTSW	4	32676786	missense	possibly damaging	0.50
R6794:Mdn1	UTSW	4	32741893	missense	probably damaging	1.00
R6894:Mdn1	UTSW	4	32748614	missense	possibly damaging	0.75
R6935:Mdn1	UTSW	4	32774041	missense	possibly damaging	0.74
R6980:Mdn1	UTSW	4	32726942	critical splice donor site	probably null
R6995:Mdn1	UTSW	4	32733374	missense	probably benign	0.03
R7048:Mdn1	UTSW	4	32767969	missense	probably benign	0.00
R7082:Mdn1	UTSW	4	32762341	missense	probably benign
R7158:Mdn1	UTSW	4	32725121	missense	probably benign	0.09
R7166:Mdn1	UTSW	4	32746446	missense	probably damaging	1.00
R7168:Mdn1	UTSW	4	32719184	missense	probably damaging	1.00
R7175:Mdn1	UTSW	4	32694634	missense	probably damaging	1.00
R7195:Mdn1	UTSW	4	32701823	missense	probably damaging	1.00
R7250:Mdn1	UTSW	4	32695427	missense	probably damaging	1.00
R7274:Mdn1	UTSW	4	32725944	missense	probably benign	0.12
R7330:Mdn1	UTSW	4	32723685	missense	probably benign	0.16
R7363:Mdn1	UTSW	4	32691729	missense	probably damaging	0.99
R7369:Mdn1	UTSW	4	32773375	missense	probably damaging	0.99
R7452:Mdn1	UTSW	4	32739030	missense	possibly damaging	0.87
R7523:Mdn1	UTSW	4	32667270	critical splice acceptor site	probably null
R7594:Mdn1	UTSW	4	32696359	missense	probably benign	0.27
R7605:Mdn1	UTSW	4	32694599	missense	probably damaging	1.00
R7661:Mdn1	UTSW	4	32691229	missense	probably benign	0.08
R7689:Mdn1	UTSW	4	32739912	missense	probably damaging	1.00
R7699:Mdn1	UTSW	4	32741344	missense	probably damaging	1.00
R7700:Mdn1	UTSW	4	32741344	missense	probably damaging	1.00
R7714:Mdn1	UTSW	4	32722360	missense	possibly damaging	0.75
R7718:Mdn1	UTSW	4	32718420	missense	probably damaging	1.00
R7762:Mdn1	UTSW	4	32734421	missense	probably benign
R7787:Mdn1	UTSW	4	32741794	missense	probably damaging	1.00
X0066:Mdn1	UTSW	4	32739030	missense	probably damaging	1.00
Z1176:Mdn1	UTSW	4	32667102	missense	probably benign	0.01
Z1176:Mdn1	UTSW	4	32668944	missense	probably damaging	1.00
Z1176:Mdn1	UTSW	4	32696244	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GCAAGGAAGCTGCTGGTTTTGTCC -3'
(R):5'- TGTAAAGTGCTCACACTCGCCC -3'

Sequencing Primer
(F):5'- TTGTCCTTGGATCCAGCACA -3'
(R):5'- AACTCTGTCTTTAGTTAGCCAGAGC -3'

Posted On

2013-05-23