Incidental Mutation 'R5328:Nt5c3b'
ID422163
Institutional Source Beutler Lab
Gene Symbol Nt5c3b
Ensembl Gene ENSMUSG00000017176
Gene Name5'-nucleotidase, cytosolic IIIB
SynonymsNt5c3l, C330027I04Rik, 2610037D24Rik
MMRRC Submission 042843-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.175) question?
Stock #R5328 (G1)
Quality Score225
Status Not validated
Chromosome11
Chromosomal Location100422321-100441808 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to G at 100440241 bp
ZygosityHeterozygous
Amino Acid Change Phenylalanine to Serine at position 42 (F42S)
Ref Sequence ENSEMBL: ENSMUSP00000103022 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000001599] [ENSMUST00000092688] [ENSMUST00000092689] [ENSMUST00000107395] [ENSMUST00000107397] [ENSMUST00000107398] [ENSMUST00000107399]
Predicted Effect probably benign
Transcript: ENSMUST00000001599
SMART Domains Protein: ENSMUSP00000001599
Gene: ENSMUSG00000001558

DomainStartEndE-ValueType
BTB 39 136 1.09e-30 SMART
BACK 141 244 6.58e-38 SMART
Kelch 292 339 2.99e-7 SMART
Kelch 340 386 2.66e-13 SMART
Kelch 387 433 1.7e-11 SMART
Kelch 434 480 1.47e-15 SMART
Kelch 481 527 2.2e-14 SMART
Kelch 528 574 3.6e-10 SMART
Predicted Effect probably damaging
Transcript: ENSMUST00000092688
AA Change: F42S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000090360
Gene: ENSMUSG00000017176
AA Change: F42S

DomainStartEndE-ValueType
Pfam:UMPH-1 44 289 6.2e-126 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000092689
SMART Domains Protein: ENSMUSP00000090361
Gene: ENSMUSG00000017176

DomainStartEndE-ValueType
Pfam:UMPH-1 1 246 1.2e-123 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000107395
SMART Domains Protein: ENSMUSP00000103018
Gene: ENSMUSG00000001558

DomainStartEndE-ValueType
BTB 39 136 1.09e-30 SMART
BACK 141 239 5.74e-29 SMART
Predicted Effect probably damaging
Transcript: ENSMUST00000107397
AA Change: F34S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000103020
Gene: ENSMUSG00000017176
AA Change: F34S

DomainStartEndE-ValueType
Pfam:UMPH-1 36 281 1.9e-123 PFAM
Predicted Effect probably damaging
Transcript: ENSMUST00000107398
AA Change: F42S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000103021
Gene: ENSMUSG00000017176
AA Change: F42S

DomainStartEndE-ValueType
Pfam:UMPH-1 44 261 3.5e-108 PFAM
Predicted Effect probably damaging
Transcript: ENSMUST00000107399
AA Change: F42S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000103022
Gene: ENSMUSG00000017176
AA Change: F42S

DomainStartEndE-ValueType
Pfam:UMPH-1 44 261 3.5e-108 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000130666
Predicted Effect noncoding transcript
Transcript: ENSMUST00000135557
Predicted Effect noncoding transcript
Transcript: ENSMUST00000140034
Predicted Effect noncoding transcript
Transcript: ENSMUST00000140381
Predicted Effect noncoding transcript
Transcript: ENSMUST00000155025
Predicted Effect noncoding transcript
Transcript: ENSMUST00000183660
Coding Region Coverage
  • 1x: 99.3%
  • 3x: 98.7%
  • 10x: 97.4%
  • 20x: 95.5%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 86 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abcb1b T A 5: 8,837,694 V860E possibly damaging Het
Abcc6 T C 7: 45,992,311 D881G probably benign Het
Abcc9 A G 6: 142,682,059 V415A probably benign Het
Adgrb3 A C 1: 25,094,275 N1003K possibly damaging Het
Adora3 C T 3: 105,907,303 T123I probably benign Het
Amotl2 A C 9: 102,723,768 T345P probably benign Het
Arap3 C T 18: 37,991,687 E247K possibly damaging Het
Atp8b1 A T 18: 64,531,391 D1235E probably benign Het
Axl A T 7: 25,773,411 V400E probably damaging Het
Brd8 T A 18: 34,607,981 N431Y probably benign Het
Cadps A T 14: 12,457,790 N1025K probably benign Het
Cblc A T 7: 19,792,580 S195T possibly damaging Het
Chd2 C T 7: 73,463,681 A1184T possibly damaging Het
Chst10 G A 1: 38,895,962 probably benign Het
Col12a1 T C 9: 79,620,060 K2663E probably damaging Het
Cspg4 A T 9: 56,885,856 I292L probably benign Het
Cul1 T C 6: 47,508,317 V294A probably damaging Het
Cyp2d11 G A 15: 82,391,771 P203L probably benign Het
Dscam T C 16: 96,673,678 H1228R probably benign Het
Eif3l A T 15: 79,093,361 K534* probably null Het
Enpep T C 3: 129,280,510 E796G probably benign Het
Ext1 C A 15: 53,075,817 W612L probably damaging Het
Faap100 T A 11: 120,377,632 E105V possibly damaging Het
Fam117a T A 11: 95,364,170 probably null Het
Fan1 A T 7: 64,354,469 Y750N probably damaging Het
Fat4 T A 3: 38,956,868 I2039N probably damaging Het
Gabrr2 G T 4: 33,082,565 D106Y probably damaging Het
Gak A C 5: 108,617,001 C145G possibly damaging Het
Galnt14 A T 17: 73,505,459 N406K possibly damaging Het
Gm10439 T G X: 149,636,163 *434E probably null Het
Gm10837 G T 14: 122,490,778 R22L unknown Het
Gm19965 T A 1: 116,821,418 H276Q possibly damaging Het
Gm43517 T C 12: 49,391,156 probably benign Het
Gm9733 T C 3: 15,332,174 M17V unknown Het
Greb1l A T 18: 10,553,720 I1574F probably damaging Het
Gzme G A 14: 56,117,767 H236Y probably benign Het
Heatr5b G A 17: 78,826,362 T266I possibly damaging Het
Hk3 T C 13: 55,013,493 I185V probably benign Het
Hnrnpr A G 4: 136,339,216 E302G probably benign Het
Itgal T C 7: 127,311,675 probably null Het
Itk A G 11: 46,331,876 S583P probably benign Het
Loxhd1 A T 18: 77,410,572 I1448F probably damaging Het
Macf1 GCCCCC GCCCCCC 4: 123,350,991 probably null Het
Man2a2 A T 7: 80,368,756 F118L probably benign Het
Mfsd5 T A 15: 102,281,012 V273E probably damaging Het
Nfe2l2 A G 2: 75,676,856 L300P probably damaging Het
Nt5c1a T G 4: 123,208,993 L122R possibly damaging Het
Olfr332 C A 11: 58,490,429 A109S possibly damaging Het
Olfr577 T A 7: 102,973,968 N8I possibly damaging Het
Pabpc1 A T 15: 36,602,877 D204E probably benign Het
Panx2 A G 15: 89,068,095 N255S probably damaging Het
Pcnt A G 10: 76,411,719 L993P probably damaging Het
Pfas A T 11: 68,988,592 C1160S probably damaging Het
Plekhh3 T A 11: 101,167,658 probably benign Het
Plod3 T A 5: 136,989,683 N258K probably damaging Het
Prr14l T C 5: 32,830,021 Q710R probably benign Het
Rnf216 G T 5: 143,092,999 T65K possibly damaging Het
Samd9l T C 6: 3,376,739 E174G probably damaging Het
Sept12 T A 16: 4,993,993 M63L possibly damaging Het
Sh3bgrl2 T A 9: 83,577,456 D22E probably benign Het
Skil A G 3: 31,117,569 K488R probably benign Het
Slamf6 T A 1: 171,938,095 I262K probably benign Het
Slc27a3 T C 3: 90,386,832 D470G probably damaging Het
Sorbs3 A T 14: 70,181,174 V680E probably damaging Het
Sox9 C T 11: 112,782,658 T25I probably benign Het
Srsf1 G T 11: 88,049,993 probably benign Het
Stard9 A C 2: 120,699,230 E1989D probably damaging Het
Thumpd2 T A 17: 81,044,162 I277F possibly damaging Het
Tinag C A 9: 77,005,631 G299* probably null Het
Tk2 A T 8: 104,229,299 probably null Het
Tmem44 A G 16: 30,540,891 S210P possibly damaging Het
Traf3ip1 C A 1: 91,520,069 P423T probably damaging Het
Trmt12 A G 15: 58,872,855 D34G probably damaging Het
Ttc39b A G 4: 83,261,941 Y72H probably damaging Het
Ttn G A 2: 76,878,411 probably benign Het
Ttyh2 T A 11: 114,710,068 I381N possibly damaging Het
Uaca A T 9: 60,870,532 N734Y probably benign Het
Usp34 T C 11: 23,464,616 I2853T probably benign Het
Usp34 G A 11: 23,488,659 G3407D probably benign Het
Vmn2r78 A G 7: 86,921,030 Y252C probably damaging Het
Wdr19 G A 5: 65,244,179 C979Y probably damaging Het
Wdr92 C A 11: 17,222,220 P103Q probably damaging Het
Yeats2 A T 16: 20,171,205 H277L probably damaging Het
Zbtb2 T C 10: 4,369,267 Y253C possibly damaging Het
Zfat C A 15: 68,179,828 G706C probably damaging Het
Zfp106 A T 2: 120,520,417 N1584K possibly damaging Het
Other mutations in Nt5c3b
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00674:Nt5c3b APN 11 100432909 splice site probably benign
IGL03060:Nt5c3b APN 11 100436219 missense probably damaging 1.00
IGL03331:Nt5c3b APN 11 100436215 missense probably damaging 1.00
R0523:Nt5c3b UTSW 11 100436210 missense probably damaging 0.99
R1678:Nt5c3b UTSW 11 100436210 missense probably damaging 0.99
R1686:Nt5c3b UTSW 11 100440094 splice site probably benign
R2042:Nt5c3b UTSW 11 100436194 missense probably benign
R4580:Nt5c3b UTSW 11 100433059 missense probably damaging 1.00
R4601:Nt5c3b UTSW 11 100432918 missense probably benign 0.20
R4735:Nt5c3b UTSW 11 100440906 missense probably benign 0.34
R5503:Nt5c3b UTSW 11 100433057 missense probably benign 0.13
R6176:Nt5c3b UTSW 11 100440148 intron probably benign
R6966:Nt5c3b UTSW 11 100429924 missense probably benign 0.04
Z1177:Nt5c3b UTSW 11 100436156 missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- CCACAGCCATGTTTGAGCAAAG -3'
(R):5'- TTGCTGCAGCTTGGTTAGAC -3'

Sequencing Primer
(F):5'- GGAATGACCCACCTCTTTGCG -3'
(R):5'- AGCTTGGTTAGACTTCCTCCG -3'
Posted On2016-08-04