Mutagenetix > Incidental Mutation

Incidental Mutation 'R5344:Hectd4'

422489

Institutional Source

Beutler Lab

Gene Symbol

Hectd4

Ensembl Gene

ENSMUSG00000042744

Gene Name

HECT domain E3 ubiquitin protein ligase 4

Synonyms

Gm15800

MMRRC Submission

042923-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.934) question?

Stock #

R5344 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

121220219-121368577 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 121343676 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 3096 (I3096T)

Ref Sequence

ENSEMBL: ENSMUSP00000048345 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000042614]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000042614
AA Change: I3096T

PolyPhen 2 Score 0.278 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000048345
Gene: ENSMUSG00000042744
AA Change: I3096T

Domain	Start	End	E-Value	Type
low complexity region	224	234	N/A	INTRINSIC
low complexity region	266	282	N/A	INTRINSIC
low complexity region	553	564	N/A	INTRINSIC
low complexity region	725	735	N/A	INTRINSIC
low complexity region	1252	1265	N/A	INTRINSIC
coiled coil region	1372	1398	N/A	INTRINSIC
low complexity region	1551	1562	N/A	INTRINSIC
low complexity region	1725	1741	N/A	INTRINSIC
low complexity region	1892	1904	N/A	INTRINSIC
low complexity region	2656	2666	N/A	INTRINSIC
low complexity region	2857	2872	N/A	INTRINSIC
low complexity region	2901	2917	N/A	INTRINSIC
low complexity region	2921	2933	N/A	INTRINSIC
low complexity region	3232	3246	N/A	INTRINSIC
low complexity region	3275	3335	N/A	INTRINSIC
low complexity region	3441	3448	N/A	INTRINSIC
low complexity region	3473	3506	N/A	INTRINSIC
low complexity region	3512	3533	N/A	INTRINSIC
low complexity region	3540	3554	N/A	INTRINSIC
low complexity region	3794	3822	N/A	INTRINSIC
HECTc	4048	4412	4.78e-11	SMART

Meta Mutation Damage Score

0.2520

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.5%
20x: 95.7%

Validation Efficiency

99% (67/68)

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2700049A03Rik	T	G	12: 71,243,027	C1499G	probably benign	Het
Aldoart1	A	G	4: 72,852,115	V152A	possibly damaging	Het
Alms1	T	C	6: 85,696,789	L3591P	probably benign	Het
Ankrd12	A	T	17: 66,049,848	M58K	probably damaging	Het
Asb3	A	C	11: 31,101,114	I523L	probably benign	Het
Ascl2	T	C	7: 142,968,699	H4R	possibly damaging	Het
Asic2	T	C	11: 80,971,587	M246V	probably damaging	Het
Btc	A	T	5: 91,376,920	C53S	possibly damaging	Het
Cdhr5	T	C	7: 141,276,524	I39M	probably damaging	Het
Cdkn3	T	A	14: 46,767,350	M123K	possibly damaging	Het
Cebpz	A	G	17: 78,926,113	Y762H	possibly damaging	Het
Ces1g	T	A	8: 93,337,193		probably benign	Het
Cfap44	T	C	16: 44,416,400		probably null	Het
Chd7	G	T	4: 8,844,417	G1537W	probably damaging	Het
Clca2	A	T	3: 145,087,942	D317E	probably damaging	Het
Clec3a	C	A	8: 114,422,972	N56K	probably damaging	Het
Col11a1	T	A	3: 114,208,362		probably null	Het
Cox20	G	A	1: 178,322,033		probably benign	Het
Cyp2d22	A	G	15: 82,371,638	V471A	possibly damaging	Het
D630045J12Rik	A	G	6: 38,158,228	V1339A	probably damaging	Het
Duox2	T	C	2: 122,281,871	D1278G	probably benign	Het
Epc1	G	A	18: 6,450,614	P284L	probably benign	Het
Evi5l	G	T	8: 4,185,990	R61L	possibly damaging	Het
Fbln2	T	C	6: 91,266,383	Y914H	probably damaging	Het
Fbxo44	A	G	4: 148,153,573	S191P	probably damaging	Het
Fign	A	G	2: 63,979,225	I567T	probably benign	Het
Fryl	C	T	5: 73,104,774	R550K	probably damaging	Het
Gpcpd1	G	A	2: 132,558,677		probably benign	Het
Hic2	T	A	16: 17,257,848	D180E	probably benign	Het
Ibtk	A	G	9: 85,735,004	F172L	possibly damaging	Het
Itga1	A	G	13: 115,002,309	S369P	possibly damaging	Het
Itgb4	G	A	11: 115,989,749	R675Q	probably null	Het
Lrrc3b	T	C	14: 15,358,591	D5G	probably damaging	Het
Maml3	T	A	3: 52,103,725	D140V	probably damaging	Het
Med21	A	G	6: 146,649,185	T65A	probably benign	Het
Mta1	T	C	12: 113,131,566		probably benign	Het
Mybpc1	T	C	10: 88,570,568	D152G	probably damaging	Het
Oas1b	C	A	5: 120,822,204	Q325K	probably benign	Het
Olfr1174-ps	A	C	2: 88,310,990	C269G	probably benign	Het
Olfr135	C	A	17: 38,209,104	N286K	probably damaging	Het
Olfr1408	A	G	1: 173,131,106	L37P	probably benign	Het
Pclo	A	G	5: 14,676,612		probably benign	Het
Phactr2	C	T	10: 13,253,616	V233I	possibly damaging	Het
Plekha2	A	T	8: 25,043,047		probably null	Het
Reg3b	A	G	6: 78,372,860	M128V	probably benign	Het
Rnaseh2a	A	G	8: 84,958,106		probably benign	Het
Scn5a	T	C	9: 119,534,007	S516G	probably benign	Het
Serpina12	T	A	12: 104,035,548		probably null	Het
Slc10a1	T	C	12: 80,953,766	T320A	possibly damaging	Het
Slc26a7	A	T	4: 14,519,402	D539E	probably benign	Het
Specc1l	C	A	10: 75,246,173	R485S	possibly damaging	Het
Srp54b	T	G	12: 55,255,581	I339S	probably damaging	Het
Tada1	G	A	1: 166,379,512		probably benign	Het
Trim16	T	C	11: 62,820,925	C54R	probably damaging	Het
Trio	C	T	15: 27,735,532	R2824Q	probably benign	Het
Ttpa	A	G	4: 20,021,245	I138V	probably damaging	Het
Ubap2	A	C	4: 41,251,578	M18R	possibly damaging	Het
Usp38	A	G	8: 80,985,763	S548P	possibly damaging	Het
Vmn2r73	T	C	7: 85,875,838	D34G	probably benign	Het
Vps13d	T	A	4: 145,178,334	H74L	probably damaging	Het
Zfp408	C	T	2: 91,645,243	C622Y	probably benign	Het
Zfp616	T	C	11: 74,084,495	I530T	possibly damaging	Het
Zfp9	C	A	6: 118,465,179	C174F	probably damaging	Het
Zfyve16	A	G	13: 92,521,588	I605T	possibly damaging	Het
Zmym5	T	C	14: 56,794,062	T530A	probably damaging	Het

Other mutations in Hectd4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00156:Hectd4	APN	5	121,363,870 (GRCm38)	missense	possibly damaging	0.51
IGL00976:Hectd4	APN	5	121,349,106 (GRCm38)	missense	probably benign	0.18
IGL01085:Hectd4	APN	5	121,331,701 (GRCm38)	missense	probably damaging	1.00
IGL01112:Hectd4	APN	5	121,306,950 (GRCm38)	missense	probably benign	0.01
IGL01402:Hectd4	APN	5	121,339,417 (GRCm38)	splice site	probably benign
IGL01474:Hectd4	APN	5	121,336,649 (GRCm38)	missense	possibly damaging	0.53
IGL01503:Hectd4	APN	5	121,318,651 (GRCm38)	missense	probably benign	0.28
IGL01548:Hectd4	APN	5	121,364,660 (GRCm38)	missense	possibly damaging	0.71
IGL01656:Hectd4	APN	5	121,322,700 (GRCm38)	missense	probably damaging	0.99
IGL01756:Hectd4	APN	5	121,344,824 (GRCm38)	missense	probably benign	0.28
IGL01819:Hectd4	APN	5	121,328,418 (GRCm38)	missense	possibly damaging	0.85
IGL02080:Hectd4	APN	5	121,366,606 (GRCm38)	utr 3 prime	probably benign
IGL02488:Hectd4	APN	5	121,292,087 (GRCm38)	missense	probably benign	0.33
IGL02490:Hectd4	APN	5	121,318,613 (GRCm38)	missense	possibly damaging	0.82
IGL02558:Hectd4	APN	5	121,344,785 (GRCm38)	missense	probably benign	0.28
IGL02626:Hectd4	APN	5	121,353,881 (GRCm38)	missense	possibly damaging	0.86
IGL02649:Hectd4	APN	5	121,349,402 (GRCm38)	missense	possibly damaging	0.73
IGL02736:Hectd4	APN	5	121,342,719 (GRCm38)	missense	possibly damaging	0.73
IGL02861:Hectd4	APN	5	121,307,004 (GRCm38)	missense	possibly damaging	0.81
IGL02880:Hectd4	APN	5	121,365,053 (GRCm38)	missense	possibly damaging	0.93
IGL02889:Hectd4	APN	5	121,365,053 (GRCm38)	missense	possibly damaging	0.93
IGL02953:Hectd4	APN	5	121,365,053 (GRCm38)	missense	possibly damaging	0.93
IGL02969:Hectd4	APN	5	121,365,053 (GRCm38)	missense	possibly damaging	0.93
IGL03031:Hectd4	APN	5	121,348,794 (GRCm38)	missense	possibly damaging	0.96
IGL03066:Hectd4	APN	5	121,365,053 (GRCm38)	missense	possibly damaging	0.93
IGL03160:Hectd4	APN	5	121,259,879 (GRCm38)	missense	probably benign
IGL03181:Hectd4	APN	5	121,353,958 (GRCm38)	missense	possibly damaging	0.91
IGL03265:Hectd4	APN	5	121,259,939 (GRCm38)	splice site	probably benign
IGL03375:Hectd4	APN	5	121,328,382 (GRCm38)	missense	possibly damaging	0.72
Achilles	UTSW	5	121,307,381 (GRCm38)	nonsense	probably null
agamemnon	UTSW	5	121,253,858 (GRCm38)	splice site	probably benign
clymnestra	UTSW	5	121,334,375 (GRCm38)	missense	possibly damaging	0.86
hector	UTSW	5	121,315,437 (GRCm38)	missense	probably damaging	1.00
helen	UTSW	5	121,310,663 (GRCm38)	missense	probably damaging	0.97
Merriwether	UTSW	5	121,353,551 (GRCm38)	missense	possibly damaging	0.53
PIT4466001:Hectd4	UTSW	5	121,333,060 (GRCm38)	critical splice donor site	probably null
R0018:Hectd4	UTSW	5	121,254,179 (GRCm38)	missense	possibly damaging	0.53
R0024:Hectd4	UTSW	5	121,308,576 (GRCm38)	missense	possibly damaging	0.92
R0030:Hectd4	UTSW	5	121,262,588 (GRCm38)	nonsense	probably null
R0080:Hectd4	UTSW	5	121,349,372 (GRCm38)	missense	probably benign	0.18
R0110:Hectd4	UTSW	5	121,305,673 (GRCm38)	missense	possibly damaging	0.53
R0110:Hectd4	UTSW	5	121,281,896 (GRCm38)	missense	possibly damaging	0.90
R0115:Hectd4	UTSW	5	121,295,506 (GRCm38)	splice site	probably benign
R0128:Hectd4	UTSW	5	121,349,243 (GRCm38)	missense	possibly damaging	0.86
R0131:Hectd4	UTSW	5	121,333,024 (GRCm38)	missense	probably benign	0.44
R0131:Hectd4	UTSW	5	121,333,024 (GRCm38)	missense	probably benign	0.44
R0132:Hectd4	UTSW	5	121,333,024 (GRCm38)	missense	probably benign	0.44
R0244:Hectd4	UTSW	5	121,329,605 (GRCm38)	missense	probably benign	0.33
R0281:Hectd4	UTSW	5	121,254,251 (GRCm38)	missense	possibly damaging	0.85
R0329:Hectd4	UTSW	5	121,259,864 (GRCm38)	missense	probably benign
R0410:Hectd4	UTSW	5	121,286,266 (GRCm38)	missense	possibly damaging	0.86
R0422:Hectd4	UTSW	5	121,343,082 (GRCm38)	splice site	probably null
R0442:Hectd4	UTSW	5	121,323,982 (GRCm38)	missense	possibly damaging	0.66
R0449:Hectd4	UTSW	5	121,364,590 (GRCm38)	splice site	probably null
R0469:Hectd4	UTSW	5	121,305,673 (GRCm38)	missense	possibly damaging	0.53
R0469:Hectd4	UTSW	5	121,281,896 (GRCm38)	missense	possibly damaging	0.90
R0481:Hectd4	UTSW	5	121,295,506 (GRCm38)	splice site	probably benign
R0510:Hectd4	UTSW	5	121,305,673 (GRCm38)	missense	possibly damaging	0.53
R0510:Hectd4	UTSW	5	121,281,896 (GRCm38)	missense	possibly damaging	0.90
R0520:Hectd4	UTSW	5	121,331,707 (GRCm38)	missense	possibly damaging	0.53
R0534:Hectd4	UTSW	5	121,348,476 (GRCm38)	missense	possibly damaging	0.96
R0603:Hectd4	UTSW	5	121,304,337 (GRCm38)	missense	possibly damaging	0.46
R0617:Hectd4	UTSW	5	121,343,232 (GRCm38)	splice site	probably benign
R0622:Hectd4	UTSW	5	121,348,625 (GRCm38)	missense	possibly damaging	0.53
R0626:Hectd4	UTSW	5	121,277,824 (GRCm38)	missense	probably benign	0.18
R0708:Hectd4	UTSW	5	121,286,463 (GRCm38)	critical splice donor site	probably null
R0710:Hectd4	UTSW	5	121,336,628 (GRCm38)	missense	probably benign	0.08
R0763:Hectd4	UTSW	5	121,307,033 (GRCm38)	unclassified	probably benign
R0764:Hectd4	UTSW	5	121,286,769 (GRCm38)	missense	possibly damaging	0.46
R1123:Hectd4	UTSW	5	121,286,736 (GRCm38)	missense	probably damaging	0.96
R1129:Hectd4	UTSW	5	121,310,599 (GRCm38)	missense	possibly damaging	0.66
R1204:Hectd4	UTSW	5	121,350,485 (GRCm38)	missense	possibly damaging	0.85
R1237:Hectd4	UTSW	5	121,321,507 (GRCm38)	missense	possibly damaging	0.90
R1257:Hectd4	UTSW	5	121,318,624 (GRCm38)	nonsense	probably null
R1391:Hectd4	UTSW	5	121,353,695 (GRCm38)	missense	possibly damaging	0.96
R1395:Hectd4	UTSW	5	121,328,513 (GRCm38)	critical splice donor site	probably null
R1468:Hectd4	UTSW	5	121,349,172 (GRCm38)	missense	possibly damaging	0.65
R1468:Hectd4	UTSW	5	121,349,172 (GRCm38)	missense	possibly damaging	0.65
R1545:Hectd4	UTSW	5	121,323,956 (GRCm38)	missense	possibly damaging	0.87
R1553:Hectd4	UTSW	5	121,349,259 (GRCm38)	missense	probably benign	0.00
R1572:Hectd4	UTSW	5	121,301,878 (GRCm38)	missense	possibly damaging	0.85
R1662:Hectd4	UTSW	5	121,317,245 (GRCm38)	missense	probably benign	0.01
R1705:Hectd4	UTSW	5	121,298,104 (GRCm38)	missense	probably benign
R1715:Hectd4	UTSW	5	121,344,818 (GRCm38)	missense	possibly damaging	0.85
R1728:Hectd4	UTSW	5	121,301,839 (GRCm38)	missense	possibly damaging	0.51
R1736:Hectd4	UTSW	5	121,349,530 (GRCm38)	missense	possibly damaging	0.53
R1768:Hectd4	UTSW	5	121,358,303 (GRCm38)	missense	possibly damaging	0.70
R1775:Hectd4	UTSW	5	121,291,191 (GRCm38)	splice site	probably benign
R1784:Hectd4	UTSW	5	121,301,839 (GRCm38)	missense	possibly damaging	0.51
R1843:Hectd4	UTSW	5	121,297,180 (GRCm38)	missense	possibly damaging	0.53
R1914:Hectd4	UTSW	5	121,322,294 (GRCm38)	missense	probably benign	0.08
R1915:Hectd4	UTSW	5	121,322,294 (GRCm38)	missense	probably benign	0.08
R2024:Hectd4	UTSW	5	121,281,918 (GRCm38)	missense	possibly damaging	0.86
R2103:Hectd4	UTSW	5	121,355,629 (GRCm38)	missense	probably benign	0.04
R2108:Hectd4	UTSW	5	121,333,424 (GRCm38)	missense	possibly damaging	0.72
R2124:Hectd4	UTSW	5	121,318,639 (GRCm38)	missense	probably damaging	0.97
R2150:Hectd4	UTSW	5	121,253,858 (GRCm38)	splice site	probably benign
R2192:Hectd4	UTSW	5	121,315,143 (GRCm38)	missense	possibly damaging	0.46
R2301:Hectd4	UTSW	5	121,353,537 (GRCm38)	missense	probably benign	0.18
R2324:Hectd4	UTSW	5	121,315,437 (GRCm38)	missense	probably damaging	1.00
R2331:Hectd4	UTSW	5	121,320,026 (GRCm38)	missense	probably benign	0.05
R2504:Hectd4	UTSW	5	121,263,967 (GRCm38)	missense	possibly damaging	0.73
R2504:Hectd4	UTSW	5	121,220,620 (GRCm38)	missense	unknown
R2904:Hectd4	UTSW	5	121,292,724 (GRCm38)	splice site	probably benign
R3843:Hectd4	UTSW	5	121,259,873 (GRCm38)	missense	possibly damaging	0.72
R3934:Hectd4	UTSW	5	121,320,101 (GRCm38)	critical splice donor site	probably null
R3944:Hectd4	UTSW	5	121,303,525 (GRCm38)	splice site	probably benign
R4133:Hectd4	UTSW	5	121,277,834 (GRCm38)	critical splice donor site	probably null
R4271:Hectd4	UTSW	5	121,220,504 (GRCm38)	small deletion	probably benign
R4413:Hectd4	UTSW	5	121,350,481 (GRCm38)	missense	possibly damaging	0.53
R4456:Hectd4	UTSW	5	121,308,271 (GRCm38)	missense	possibly damaging	0.65
R4489:Hectd4	UTSW	5	121,286,257 (GRCm38)	missense	possibly damaging	0.73
R4539:Hectd4	UTSW	5	121,314,907 (GRCm38)	nonsense	probably null
R4564:Hectd4	UTSW	5	121,350,431 (GRCm38)	missense	probably benign	0.33
R4582:Hectd4	UTSW	5	121,286,419 (GRCm38)	missense	possibly damaging	0.53
R4629:Hectd4	UTSW	5	121,297,203 (GRCm38)	missense	probably benign	0.01
R4633:Hectd4	UTSW	5	121,349,216 (GRCm38)	missense	probably benign	0.33
R4643:Hectd4	UTSW	5	121,349,055 (GRCm38)	missense	possibly damaging	0.53
R4679:Hectd4	UTSW	5	121,325,251 (GRCm38)	missense	possibly damaging	0.72
R4681:Hectd4	UTSW	5	121,303,615 (GRCm38)	missense	possibly damaging	0.86
R4734:Hectd4	UTSW	5	121,341,977 (GRCm38)	missense	possibly damaging	0.53
R4739:Hectd4	UTSW	5	121,348,442 (GRCm38)	missense	probably benign
R4781:Hectd4	UTSW	5	121,306,107 (GRCm38)	critical splice donor site	probably null
R4860:Hectd4	UTSW	5	121,305,818 (GRCm38)	missense	probably benign	0.04
R4860:Hectd4	UTSW	5	121,305,818 (GRCm38)	missense	probably benign	0.04
R4869:Hectd4	UTSW	5	121,322,672 (GRCm38)	missense	possibly damaging	0.46
R4909:Hectd4	UTSW	5	121,263,891 (GRCm38)	missense	probably benign	0.18
R4922:Hectd4	UTSW	5	121,359,315 (GRCm38)	missense	possibly damaging	0.86
R4925:Hectd4	UTSW	5	121,322,690 (GRCm38)	missense	possibly damaging	0.83
R5004:Hectd4	UTSW	5	121,329,565 (GRCm38)	missense	possibly damaging	0.93
R5004:Hectd4	UTSW	5	121,328,199 (GRCm38)	splice site	probably null
R5129:Hectd4	UTSW	5	121,343,510 (GRCm38)	missense	possibly damaging	0.87
R5217:Hectd4	UTSW	5	121,353,551 (GRCm38)	missense	possibly damaging	0.53
R5267:Hectd4	UTSW	5	121,344,824 (GRCm38)	missense	probably benign	0.28
R5345:Hectd4	UTSW	5	121,263,974 (GRCm38)	missense	possibly damaging	0.85
R5347:Hectd4	UTSW	5	121,304,448 (GRCm38)	missense	probably benign	0.33
R5360:Hectd4	UTSW	5	121,315,401 (GRCm38)	missense	possibly damaging	0.90
R5363:Hectd4	UTSW	5	121,310,603 (GRCm38)	missense	probably benign	0.04
R5445:Hectd4	UTSW	5	121,266,274 (GRCm38)	missense	probably benign	0.00
R5479:Hectd4	UTSW	5	121,306,948 (GRCm38)	missense	probably benign
R5507:Hectd4	UTSW	5	121,281,101 (GRCm38)	missense	unknown
R5552:Hectd4	UTSW	5	121,342,851 (GRCm38)	missense	possibly damaging	0.96
R5691:Hectd4	UTSW	5	121,348,815 (GRCm38)	missense	possibly damaging	0.85
R5745:Hectd4	UTSW	5	121,353,502 (GRCm38)	missense	possibly damaging	0.96
R5757:Hectd4	UTSW	5	121,348,619 (GRCm38)	missense	possibly damaging	0.72
R5845:Hectd4	UTSW	5	121,307,524 (GRCm38)	critical splice donor site	probably null
R5869:Hectd4	UTSW	5	121,343,225 (GRCm38)	critical splice donor site	probably null
R5913:Hectd4	UTSW	5	121,323,974 (GRCm38)	missense	possibly damaging	0.83
R5920:Hectd4	UTSW	5	121,308,271 (GRCm38)	missense	possibly damaging	0.65
R5943:Hectd4	UTSW	5	121,322,294 (GRCm38)	missense	probably benign	0.01
R6219:Hectd4	UTSW	5	121,308,878 (GRCm38)	missense	possibly damaging	0.92
R6250:Hectd4	UTSW	5	121,339,498 (GRCm38)	missense	possibly damaging	0.85
R6301:Hectd4	UTSW	5	121,254,220 (GRCm38)	missense	possibly damaging	0.91
R6428:Hectd4	UTSW	5	121,350,445 (GRCm38)	missense	possibly damaging	0.53
R6446:Hectd4	UTSW	5	121,334,375 (GRCm38)	missense	possibly damaging	0.86
R6453:Hectd4	UTSW	5	121,350,592 (GRCm38)	missense	probably damaging	1.00
R6513:Hectd4	UTSW	5	121,356,196 (GRCm38)	splice site	probably null
R6540:Hectd4	UTSW	5	121,303,571 (GRCm38)	missense	probably benign	0.33
R6706:Hectd4	UTSW	5	121,320,084 (GRCm38)	missense	possibly damaging	0.92
R6720:Hectd4	UTSW	5	121,307,381 (GRCm38)	nonsense	probably null
R6736:Hectd4	UTSW	5	121,277,725 (GRCm38)	missense	possibly damaging	0.86
R6776:Hectd4	UTSW	5	121,353,511 (GRCm38)	missense	possibly damaging	0.85
R7033:Hectd4	UTSW	5	121,364,568 (GRCm38)	missense	possibly damaging	0.86
R7038:Hectd4	UTSW	5	121,299,597 (GRCm38)	missense	possibly damaging	0.90
R7175:Hectd4	UTSW	5	121,273,629 (GRCm38)	missense	possibly damaging	0.85
R7180:Hectd4	UTSW	5	121,308,342 (GRCm38)	missense	probably benign	0.01
R7234:Hectd4	UTSW	5	121,329,073 (GRCm38)	missense	possibly damaging	0.53
R7253:Hectd4	UTSW	5	121,314,881 (GRCm38)	missense	possibly damaging	0.66
R7349:Hectd4	UTSW	5	121,310,663 (GRCm38)	missense	probably damaging	0.97
R7450:Hectd4	UTSW	5	121,281,932 (GRCm38)	missense	probably benign	0.00
R7467:Hectd4	UTSW	5	121,323,961 (GRCm38)	missense	possibly damaging	0.66
R7475:Hectd4	UTSW	5	121,358,133 (GRCm38)	splice site	probably null
R7482:Hectd4	UTSW	5	121,363,878 (GRCm38)	missense	possibly damaging	0.71
R7512:Hectd4	UTSW	5	121,297,109 (GRCm38)	missense	possibly damaging	0.72
R7525:Hectd4	UTSW	5	121,343,665 (GRCm38)	missense	possibly damaging	0.70
R7559:Hectd4	UTSW	5	121,315,510 (GRCm38)	splice site	probably null
R7560:Hectd4	UTSW	5	121,254,342 (GRCm38)	missense	possibly damaging	0.53
R7561:Hectd4	UTSW	5	121,291,225 (GRCm38)	missense	possibly damaging	0.91
R7576:Hectd4	UTSW	5	121,349,459 (GRCm38)	missense	possibly damaging	0.91
R7584:Hectd4	UTSW	5	121,318,735 (GRCm38)	missense	possibly damaging	0.83
R7648:Hectd4	UTSW	5	121,254,371 (GRCm38)	missense	possibly damaging	0.73
R7663:Hectd4	UTSW	5	121,324,031 (GRCm38)	missense	probably benign	0.06
R7692:Hectd4	UTSW	5	121,321,564 (GRCm38)	missense	possibly damaging	0.46
R7725:Hectd4	UTSW	5	121,220,617 (GRCm38)	missense	unknown
R7731:Hectd4	UTSW	5	121,307,014 (GRCm38)	missense	probably benign	0.00
R7732:Hectd4	UTSW	5	121,336,629 (GRCm38)	missense	probably benign	0.14
R7782:Hectd4	UTSW	5	121,305,721 (GRCm38)	missense	possibly damaging	0.53
R7854:Hectd4	UTSW	5	121,329,568 (GRCm38)	missense	probably benign	0.27
R7898:Hectd4	UTSW	5	121,331,817 (GRCm38)	missense	probably benign	0.18
R7910:Hectd4	UTSW	5	121,254,228 (GRCm38)	missense	possibly damaging	0.86
R7962:Hectd4	UTSW	5	121,310,629 (GRCm38)	missense	probably damaging	0.98
R8003:Hectd4	UTSW	5	121,339,518 (GRCm38)	missense	possibly damaging	0.85
R8098:Hectd4	UTSW	5	121,321,398 (GRCm38)	missense	possibly damaging	0.46
R8110:Hectd4	UTSW	5	121,332,949 (GRCm38)	missense	possibly damaging	0.96
R8118:Hectd4	UTSW	5	121,286,376 (GRCm38)	missense	probably benign	0.33
R8171:Hectd4	UTSW	5	121,318,756 (GRCm38)	missense	possibly damaging	0.82
R8234:Hectd4	UTSW	5	121,339,544 (GRCm38)	missense	possibly damaging	0.72
R8289:Hectd4	UTSW	5	121,266,361 (GRCm38)	missense	possibly damaging	0.53
R8292:Hectd4	UTSW	5	121,317,225 (GRCm38)	missense	possibly damaging	0.66
R8348:Hectd4	UTSW	5	121,220,256 (GRCm38)	start gained	probably benign
R8397:Hectd4	UTSW	5	121,259,894 (GRCm38)	missense	probably damaging	0.98
R8436:Hectd4	UTSW	5	121,308,358 (GRCm38)	missense	possibly damaging	0.90
R8436:Hectd4	UTSW	5	121,343,147 (GRCm38)	missense	probably benign	0.00
R8443:Hectd4	UTSW	5	121,329,109 (GRCm38)	missense	possibly damaging	0.72
R8448:Hectd4	UTSW	5	121,220,256 (GRCm38)	start gained	probably benign
R8516:Hectd4	UTSW	5	121,349,010 (GRCm38)	missense	possibly damaging	0.53
R8519:Hectd4	UTSW	5	121,304,426 (GRCm38)	nonsense	probably null
R8553:Hectd4	UTSW	5	121,353,598 (GRCm38)	missense	possibly damaging	0.73
R8557:Hectd4	UTSW	5	121,310,651 (GRCm38)	missense	possibly damaging	0.66
R8725:Hectd4	UTSW	5	121,350,494 (GRCm38)	missense	probably damaging	1.00
R8751:Hectd4	UTSW	5	121,363,775 (GRCm38)	nonsense	probably null
R8769:Hectd4	UTSW	5	121,281,873 (GRCm38)	missense	possibly damaging	0.53
R8803:Hectd4	UTSW	5	121,323,931 (GRCm38)	missense	probably benign	0.01
R8887:Hectd4	UTSW	5	121,295,478 (GRCm38)	missense	probably benign	0.44
R8982:Hectd4	UTSW	5	121,328,242 (GRCm38)	missense	probably benign	0.02
R8988:Hectd4	UTSW	5	121,277,756 (GRCm38)	missense	possibly damaging	0.86
R8991:Hectd4	UTSW	5	121,358,284 (GRCm38)	missense	probably benign	0.33
R8994:Hectd4	UTSW	5	121,303,566 (GRCm38)	missense	probably benign	0.33
R8995:Hectd4	UTSW	5	121,254,359 (GRCm38)	missense	possibly damaging	0.96
R9049:Hectd4	UTSW	5	121,313,892 (GRCm38)	missense	possibly damaging	0.92
R9093:Hectd4	UTSW	5	121,273,614 (GRCm38)	missense	probably benign	0.14
R9106:Hectd4	UTSW	5	121,329,556 (GRCm38)	missense	possibly damaging	0.53
R9137:Hectd4	UTSW	5	121,358,175 (GRCm38)	missense	possibly damaging	0.53
R9146:Hectd4	UTSW	5	121,349,034 (GRCm38)	missense	probably benign	0.33
R9154:Hectd4	UTSW	5	121,253,904 (GRCm38)	missense
R9162:Hectd4	UTSW	5	121,306,979 (GRCm38)	missense	possibly damaging	0.66
R9166:Hectd4	UTSW	5	121,308,627 (GRCm38)	missense	probably damaging	0.96
R9183:Hectd4	UTSW	5	121,299,488 (GRCm38)	missense	possibly damaging	0.51
R9207:Hectd4	UTSW	5	121,295,433 (GRCm38)	missense	possibly damaging	0.86
R9291:Hectd4	UTSW	5	121,348,965 (GRCm38)	missense	probably benign	0.14
R9300:Hectd4	UTSW	5	121,348,889 (GRCm38)	missense	probably benign	0.33
R9314:Hectd4	UTSW	5	121,299,645 (GRCm38)	critical splice donor site	probably null
R9381:Hectd4	UTSW	5	121,334,429 (GRCm38)	missense	possibly damaging	0.53
R9432:Hectd4	UTSW	5	121,322,801 (GRCm38)	missense	probably benign	0.01
R9491:Hectd4	UTSW	5	121,314,918 (GRCm38)	missense	probably damaging	0.97
R9532:Hectd4	UTSW	5	121,364,553 (GRCm38)	missense	probably benign	0.00
R9557:Hectd4	UTSW	5	121,321,554 (GRCm38)	missense	possibly damaging	0.66
R9561:Hectd4	UTSW	5	121,334,469 (GRCm38)	missense	possibly damaging	0.53
R9593:Hectd4	UTSW	5	121,286,781 (GRCm38)	nonsense	probably null
R9704:Hectd4	UTSW	5	121,310,681 (GRCm38)	missense	probably benign	0.00
R9705:Hectd4	UTSW	5	121,310,681 (GRCm38)	missense	probably benign	0.00
R9712:Hectd4	UTSW	5	121,310,681 (GRCm38)	missense	probably benign	0.00
R9713:Hectd4	UTSW	5	121,310,681 (GRCm38)	missense	probably benign	0.00
R9726:Hectd4	UTSW	5	121,310,681 (GRCm38)	missense	probably benign	0.00
R9732:Hectd4	UTSW	5	121,254,191 (GRCm38)	nonsense	probably null
R9750:Hectd4	UTSW	5	121,310,681 (GRCm38)	missense	probably benign	0.00
R9752:Hectd4	UTSW	5	121,334,352 (GRCm38)	missense	possibly damaging	0.85
R9752:Hectd4	UTSW	5	121,310,681 (GRCm38)	missense	probably benign	0.00
R9772:Hectd4	UTSW	5	121,310,681 (GRCm38)	missense	probably benign	0.00
X0026:Hectd4	UTSW	5	121,349,637 (GRCm38)	missense	probably benign	0.04
X0027:Hectd4	UTSW	5	121,321,404 (GRCm38)	missense	probably benign	0.27
Z1088:Hectd4	UTSW	5	121,295,503 (GRCm38)	splice site	probably null
Z1177:Hectd4	UTSW	5	121,358,320 (GRCm38)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- CCGATGACTTATGCCCAGTG -3'
(R):5'- TAGAAGGCCATCCCTCTGTG -3'

Sequencing Primer
(F):5'- ATGGCCTGGTCAAGTCTGTCTTAAC -3'
(R):5'- ATCCCTCTGTGCCTGGCAG -3'

Posted On

2016-08-04