|Institutional Source||Beutler Lab|
|Gene Name||Rho-associated coiled-coil containing protein kinase 2|
|Synonyms||B230113H15Rik, ROKalpha, Rho-kinase, Rock-II, Rock2m|
|Is this an essential gene?||Probably essential (E-score: 0.847)|
|Stock #||R5363 (G1)|
|Chromosomal Location||16894895-16987823 bp(+) (GRCm38)|
|Type of Mutation||critical splice donor site (2 bp from exon)|
|DNA Base Change (assembly)||T to C at 16965654 bp (GRCm38)|
|Amino Acid Change|
|Ref Sequence||ENSEMBL: ENSMUSP00000152813 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000020904] [ENSMUST00000020904] [ENSMUST00000220688]|
|AlphaFold||no structure available at present|
|Coding Region Coverage||
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for disruptions in this genes tend to die before birth; those that survive are small. Hemorrhaging occurs in the placenta, at the tips of hind limb buds and occasionally the tail. Subsequent development is normal and the size deficit is made up. They are fertile as adults. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Rock2||
(F):5'- AACTTGCTTTATTGCGGAGGTC -3'
(R):5'- GCTGGCTCTGGAATTCAAATCTC -3'
(F):5'- CGGAGGTCTAGTGGTGAATGC -3'
(R):5'- GGAATTCAAATCTCTTGTTTCACCC -3'