Mutagenetix > Incidental Mutation

Incidental Mutation 'R5364:Lyst'

423041

Institutional Source

Beutler Lab

Gene Symbol

Lyst

Ensembl Gene

ENSMUSG00000019726

Gene Name

lysosomal trafficking regulator

Synonyms

D13Sfk13

MMRRC Submission

042942-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.298) question?

Stock #

R5364 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

13590397-13778803 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 13656854 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 1621 (D1621G)

Ref Sequence

ENSEMBL: ENSMUSP00000106188 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000110559]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000110559
AA Change: D1621G

PolyPhen 2 Score 0.349 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000106188
Gene: ENSMUSG00000019726
AA Change: D1621G

Domain	Start	End	E-Value	Type
low complexity region	26	36	N/A	INTRINSIC
low complexity region	72	82	N/A	INTRINSIC
low complexity region	399	412	N/A	INTRINSIC
low complexity region	1333	1344	N/A	INTRINSIC
low complexity region	2295	2307	N/A	INTRINSIC
low complexity region	2427	2445	N/A	INTRINSIC
low complexity region	2534	2546	N/A	INTRINSIC
Pfam:PH_BEACH	3006	3101	5.8e-25	PFAM
Beach	3118	3408	1.25e-193	SMART
Blast:Beach	3441	3478	9e-13	BLAST
WD40	3539	3579	5.75e-1	SMART
WD40	3591	3630	2.89e-5	SMART
WD40	3633	3676	1.38e0	SMART
WD40	3724	3765	1.27e-1	SMART

Meta Mutation Damage Score

0.1580

Coding Region Coverage

1x: 99.3%
3x: 98.8%
10x: 97.6%
20x: 96.3%

Validation Efficiency

100% (102/102)

MGI Phenotype

Strain: 1855968
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that regulates intracellular protein trafficking in endosomes, and may be involved in pigmentation. Mutations in this gene are associated with Chediak-Higashi syndrome, a lysosomal storage disorder. Alternative splicing results in multiple transcript variants, though the full-length nature of some of these variants has not been determined. [provided by RefSeq, Apr 2013]
PHENOTYPE: Homozygous mice have a phenotype similar to human Chediak-Higashi syndrome patients, exhibiting lysosomal dysfunction with resultant protein storage; diluted coat color; abnormal melanogenesis; immune cell dysfunction resulting in increased susceptibility to bacterial, viral, and parasitic infections and decreased cytotoxic activity against tumor cells. [provided by MGI curators]

Allele List at MGI

All alleles(52) : Targeted(3) Gene trapped(34) Spontaneous(8) Chemically induced(6) Radiation induced(1)

Other mutations in this stock

Total: 91 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310022A10Rik	C	G	7: 27,578,767 (GRCm38)	T242R	probably damaging	Het
Abcc10	G	A	17: 46,305,651 (GRCm38)	R1205C	probably benign	Het
Acer1	A	G	17: 56,982,000 (GRCm38)	F37L	probably damaging	Het
Acp7	C	A	7: 28,611,023 (GRCm38)	G463V	probably benign	Het
Actr2	A	T	11: 20,100,797 (GRCm38)		probably benign	Het
Adam15	A	T	3: 89,345,595 (GRCm38)	I272K	probably damaging	Het
Adam1b	T	A	5: 121,500,883 (GRCm38)	I700F	possibly damaging	Het
Adam33	A	G	2: 131,054,472 (GRCm38)		probably null	Het
Ano1	T	C	7: 144,637,204 (GRCm38)	Y380C	probably damaging	Het
Arfgap3	A	C	15: 83,314,361 (GRCm38)	M307R	probably damaging	Het
Arhgap21	T	A	2: 20,849,722 (GRCm38)	R1610W	probably damaging	Het
Bbs2	A	G	8: 94,074,395 (GRCm38)	Y603H	probably benign	Het
Bbs9	G	T	9: 22,575,196 (GRCm38)		probably null	Het
Bcar3	A	C	3: 122,529,632 (GRCm38)	M779L	probably benign	Het
Bub3	A	T	7: 131,560,738 (GRCm38)	N10I	possibly damaging	Het
Cacna1c	T	G	6: 118,656,543 (GRCm38)	E1098D	probably benign	Het
Cacna1g	T	A	11: 94,416,858 (GRCm38)	M1738L	probably benign	Het
Camk2d	G	T	3: 126,780,420 (GRCm38)	G159C	probably damaging	Het
Ccdc51	A	T	9: 109,092,120 (GRCm38)	E358D	possibly damaging	Het
Cdc42bpa	A	G	1: 180,067,182 (GRCm38)	D309G	probably benign	Het
Cdhr3	A	T	12: 33,051,008 (GRCm38)	F468I	possibly damaging	Het
Chrd	A	G	16: 20,733,148 (GRCm38)	M1V	probably null	Het
Cmtm1	CGGCACGTACTGAAGGTCGCTGACTGGATGGTGTGGCACGTACTGAAGGTCGCTGACTGGATGGTGTGGCACGTACTGAAGGTCGCTGACTGGATGGT	CGGCACGTACTGAAGGTCGCTGACTGGATGGTGTGGCACGTACTGAAGGTCGCTGACTGGATGGT	8: 104,309,470 (GRCm38)		probably benign	Het
Dcdc2b	T	C	4: 129,609,170 (GRCm38)	Y253C	probably damaging	Het
Dclk1	A	C	3: 55,255,945 (GRCm38)	N153H	possibly damaging	Het
Dgkg	G	T	16: 22,600,461 (GRCm38)	S96R	probably benign	Het
Dnah9	A	G	11: 65,881,696 (GRCm38)	Y3737H	possibly damaging	Het
Elovl4	A	T	9: 83,790,023 (GRCm38)	I81N	probably benign	Het
Epha7	T	A	4: 28,950,557 (GRCm38)	Y791N	probably damaging	Het
Fam193a	C	A	5: 34,466,253 (GRCm38)	T1395N	probably benign	Het
Fbln5	A	T	12: 101,771,364 (GRCm38)	V141E	probably damaging	Het
Flii	T	C	11: 60,720,128 (GRCm38)	T492A	probably benign	Het
Fnip2	A	G	3: 79,481,168 (GRCm38)	I752T	probably benign	Het
Fpr3	T	C	17: 17,970,544 (GRCm38)	W26R	probably benign	Het
Gabrb1	A	T	5: 72,136,762 (GRCm38)	T460S	probably benign	Het
Gde1	T	C	7: 118,698,651 (GRCm38)	N4S	probably benign	Het
Ghitm	A	T	14: 37,125,217 (GRCm38)	I300N	probably damaging	Het
Ghitm	G	T	14: 37,125,199 (GRCm38)	T306K	probably benign	Het
Gm4787	G	C	12: 81,377,830 (GRCm38)	T518S	probably benign	Het
Iqcd	T	C	5: 120,600,267 (GRCm38)	I50T	probably damaging	Het
Itpripl1	G	A	2: 127,141,819 (GRCm38)	P128S	possibly damaging	Het
Jag2	C	A	12: 112,910,534 (GRCm38)	L1000F	probably damaging	Het
Klhdc4	A	T	8: 121,806,636 (GRCm38)		probably benign	Het
Klra5	A	G	6: 129,899,353 (GRCm38)	F164L	probably benign	Het
Larp1b	A	G	3: 40,977,223 (GRCm38)	Y288C	probably damaging	Het
Lrfn3	T	A	7: 30,355,653 (GRCm38)	E622D	possibly damaging	Het
Mastl	T	C	2: 23,133,653 (GRCm38)	T353A	probably benign	Het
Mkln1	T	C	6: 31,496,712 (GRCm38)	Y130H	probably damaging	Het
Mms22l	T	A	4: 24,496,882 (GRCm38)		probably benign	Het
Mroh7	T	A	4: 106,691,643 (GRCm38)	M1008L	probably benign	Het
Nipal1	T	C	5: 72,667,900 (GRCm38)	V312A	probably damaging	Het
Nlrp5	T	A	7: 23,418,328 (GRCm38)	Y492*	probably null	Het
Odad1	T	A	7: 45,936,332 (GRCm38)	I105N	probably damaging	Het
Or1n1	T	C	2: 36,859,994 (GRCm38)	Y118C	probably damaging	Het
Otulinl	G	A	15: 27,659,945 (GRCm38)	Q24*	probably null	Het
Pcdhb11	T	A	18: 37,422,179 (GRCm38)	D187E	probably benign	Het
Pcdhb13	A	G	18: 37,443,508 (GRCm38)	Y313C	probably damaging	Het
Pdpn	G	A	4: 143,273,956 (GRCm38)	T102I	possibly damaging	Het
Pear1	A	T	3: 87,758,361 (GRCm38)	C120S	probably damaging	Het
Peg10	T	C	6: 4,756,128 (GRCm38)		probably benign	Het
Ppm1e	A	G	11: 87,237,181 (GRCm38)	W384R	probably benign	Het
Ppp1r10	C	T	17: 35,930,432 (GRCm38)	P700S	unknown	Het
Prl2c5	G	A	13: 13,183,042 (GRCm38)	R13K	probably benign	Het
Prmt3	C	A	7: 49,848,806 (GRCm38)	P487T	probably damaging	Het
Proser3	C	A	7: 30,546,148 (GRCm38)	A144S	possibly damaging	Het
Ptcd1	G	A	5: 145,151,431 (GRCm38)	T590I	probably damaging	Het
Rbsn	A	G	6: 92,193,977 (GRCm38)	V321A	probably damaging	Het
Slc40a1	C	A	1: 45,925,223 (GRCm38)	C14F	probably damaging	Het
Slc6a15	T	C	10: 103,393,508 (GRCm38)	I136T	probably damaging	Het
Slc7a4	A	G	16: 17,573,363 (GRCm38)	I449T	probably benign	Het
Snrnp48	T	A	13: 38,210,189 (GRCm38)		probably null	Het
Tada2a	A	G	11: 84,121,147 (GRCm38)	Y23H	probably benign	Het
Tbx15	A	T	3: 99,352,192 (GRCm38)	S460C	possibly damaging	Het
Tbx21	C	T	11: 97,101,478 (GRCm38)		probably null	Het
Tmcc2	T	G	1: 132,357,796 (GRCm38)	T376P	probably damaging	Het
Tmco4	G	A	4: 139,052,504 (GRCm38)	C420Y	probably damaging	Het
Tmem235	C	A	11: 117,864,194 (GRCm38)	Y157*	probably null	Het
Tmem63b	T	C	17: 45,664,727 (GRCm38)		probably benign	Het
Tnfrsf1a	T	C	6: 125,357,393 (GRCm38)	S92P	possibly damaging	Het
Top3b	A	T	16: 16,886,970 (GRCm38)	T397S	probably benign	Het
Trabd	C	A	15: 89,082,804 (GRCm38)		probably benign	Het
Trbv21	A	T	6: 41,202,830 (GRCm38)	I27L	possibly damaging	Het
Trim3	C	T	7: 105,619,069 (GRCm38)	V169M	probably damaging	Het
Ttn	T	C	2: 76,977,114 (GRCm38)	T92A	probably damaging	Het
Ttn	C	T	2: 76,908,516 (GRCm38)	S3893N	probably benign	Het
Uchl4	A	T	9: 64,235,539 (GRCm38)	I101F	possibly damaging	Het
Vmn1r5	T	A	6: 56,985,598 (GRCm38)	M86K	probably damaging	Het
Vmn2r55	T	A	7: 12,670,903 (GRCm38)	Q191L	possibly damaging	Het
Zfp458	A	T	13: 67,257,948 (GRCm38)	C139*	probably null	Het
Zfp788	T	C	7: 41,650,127 (GRCm38)	L729P	probably damaging	Het
Zmym2	T	A	14: 56,920,645 (GRCm38)	M547K	possibly damaging	Het

Other mutations in Lyst

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00156:Lyst	APN	13	13,648,878 (GRCm38)	missense	probably benign
IGL00474:Lyst	APN	13	13,643,536 (GRCm38)	missense	possibly damaging	0.48
IGL00484:Lyst	APN	13	13,709,603 (GRCm38)	missense	probably benign	0.02
IGL00492:Lyst	APN	13	13,678,175 (GRCm38)	missense	possibly damaging	0.54
IGL00807:Lyst	APN	13	13,650,423 (GRCm38)	missense	possibly damaging	0.91
IGL00949:Lyst	APN	13	13,635,485 (GRCm38)	missense	possibly damaging	0.87
IGL00952:Lyst	APN	13	13,678,107 (GRCm38)	missense	probably benign	0.05
IGL01305:Lyst	APN	13	13,678,056 (GRCm38)	missense	probably benign	0.01
IGL01317:Lyst	APN	13	13,670,870 (GRCm38)	missense	probably benign
IGL01419:Lyst	APN	13	13,635,838 (GRCm38)	missense	probably benign	0.00
IGL01445:Lyst	APN	13	13,651,714 (GRCm38)	missense	probably benign	0.00
IGL01690:Lyst	APN	13	13,743,246 (GRCm38)	missense	probably damaging	1.00
IGL01791:Lyst	APN	13	13,635,302 (GRCm38)	missense	probably damaging	1.00
IGL01809:Lyst	APN	13	13,637,803 (GRCm38)	missense	probably damaging	1.00
IGL01896:Lyst	APN	13	13,635,577 (GRCm38)	missense	probably benign	0.04
IGL01938:Lyst	APN	13	13,637,424 (GRCm38)	missense	possibly damaging	0.93
IGL01986:Lyst	APN	13	13,775,627 (GRCm38)	critical splice donor site	probably null
IGL02022:Lyst	APN	13	13,664,044 (GRCm38)	nonsense	probably null
IGL02044:Lyst	APN	13	13,712,846 (GRCm38)	missense	probably damaging	1.00
IGL02157:Lyst	APN	13	13,660,956 (GRCm38)	missense	probably benign
IGL02185:Lyst	APN	13	13,661,093 (GRCm38)	nonsense	probably null
IGL02215:Lyst	APN	13	13,660,956 (GRCm38)	missense	probably benign
IGL02245:Lyst	APN	13	13,660,956 (GRCm38)	missense	probably benign
IGL02246:Lyst	APN	13	13,660,956 (GRCm38)	missense	probably benign
IGL02247:Lyst	APN	13	13,660,956 (GRCm38)	missense	probably benign
IGL02297:Lyst	APN	13	13,638,092 (GRCm38)	nonsense	probably null
IGL02411:Lyst	APN	13	13,660,956 (GRCm38)	missense	probably benign
IGL02415:Lyst	APN	13	13,660,956 (GRCm38)	missense	probably benign
IGL02419:Lyst	APN	13	13,660,956 (GRCm38)	missense	probably benign
IGL02420:Lyst	APN	13	13,660,956 (GRCm38)	missense	probably benign
IGL02429:Lyst	APN	13	13,660,956 (GRCm38)	missense	probably benign
IGL02501:Lyst	APN	13	13,711,645 (GRCm38)	missense	probably benign	0.02
IGL02522:Lyst	APN	13	13,634,705 (GRCm38)	missense	possibly damaging	0.81
IGL02535:Lyst	APN	13	13,650,342 (GRCm38)	missense	probably benign	0.00
IGL02596:Lyst	APN	13	13,660,956 (GRCm38)	missense	probably benign
IGL02601:Lyst	APN	13	13,660,956 (GRCm38)	missense	probably benign
IGL02603:Lyst	APN	13	13,660,956 (GRCm38)	missense	probably benign
IGL02608:Lyst	APN	13	13,712,754 (GRCm38)	missense	probably damaging	0.98
IGL02622:Lyst	APN	13	13,681,390 (GRCm38)	missense	probably damaging	1.00
IGL02690:Lyst	APN	13	13,641,125 (GRCm38)	missense	possibly damaging	0.58
IGL02715:Lyst	APN	13	13,674,320 (GRCm38)	splice site	probably null
IGL02725:Lyst	APN	13	13,760,827 (GRCm38)	missense	probably damaging	1.00
IGL02729:Lyst	APN	13	13,746,609 (GRCm38)	missense	possibly damaging	0.95
IGL02729:Lyst	APN	13	13,674,339 (GRCm38)	missense	possibly damaging	0.81
IGL02820:Lyst	APN	13	13,638,058 (GRCm38)	missense	probably benign	0.03
IGL02945:Lyst	APN	13	13,761,198 (GRCm38)	missense	possibly damaging	0.48
IGL02981:Lyst	APN	13	13,634,911 (GRCm38)	missense	probably damaging	0.99
IGL03087:Lyst	APN	13	13,635,056 (GRCm38)	missense	probably damaging	1.00
IGL03149:Lyst	APN	13	13,681,444 (GRCm38)	missense	probably benign	0.14
IGL03158:Lyst	APN	13	13,651,752 (GRCm38)	critical splice donor site	probably null
IGL03226:Lyst	APN	13	13,709,559 (GRCm38)	missense	probably benign	0.01
IGL03242:Lyst	APN	13	13,656,881 (GRCm38)	nonsense	probably null
IGL03385:Lyst	APN	13	13,656,980 (GRCm38)	nonsense	probably null
50-cal	UTSW	13	13,708,212 (GRCm38)	critical splice donor site	probably null
charcoal	UTSW	13	13,696,761 (GRCm38)	nonsense	probably null
charlotte_gray	UTSW	13	13,602,026 (GRCm38)	intron	probably benign
charzard	UTSW	13	13,647,083 (GRCm38)	nonsense	probably null
grey_wolf	UTSW	13	0 ()	unclassified
lightspeed	UTSW	13	13,740,536 (GRCm38)	missense	possibly damaging	0.91
pardon	UTSW	13	13,677,952 (GRCm38)	missense	probably benign	0.00
robin	UTSW	13	13,648,802 (GRCm38)	nonsense	probably null
sooty	UTSW	13	0 ()	unclassified
souris	UTSW	13	13,683,223 (GRCm38)	unclassified	probably benign
Swallow	UTSW	13	13,757,422 (GRCm38)	missense	probably benign	0.00
vulpix	UTSW	13	13,696,794 (GRCm38)	splice site	probably null
ANU22:Lyst	UTSW	13	13,678,056 (GRCm38)	missense	probably benign	0.01
IGL02835:Lyst	UTSW	13	13,661,100 (GRCm38)	missense	possibly damaging	0.82
P0031:Lyst	UTSW	13	13,664,031 (GRCm38)	missense	probably damaging	1.00
R0012:Lyst	UTSW	13	13,687,694 (GRCm38)	missense	probably benign	0.10
R0012:Lyst	UTSW	13	13,687,694 (GRCm38)	missense	probably benign	0.10
R0031:Lyst	UTSW	13	13,708,156 (GRCm38)	missense	probably benign	0.14
R0115:Lyst	UTSW	13	13,677,952 (GRCm38)	missense	probably benign	0.00
R0212:Lyst	UTSW	13	13,635,985 (GRCm38)	missense	possibly damaging	0.93
R0386:Lyst	UTSW	13	13,708,214 (GRCm38)	splice site	probably benign
R0393:Lyst	UTSW	13	13,647,079 (GRCm38)	missense	probably benign	0.01
R0415:Lyst	UTSW	13	13,711,610 (GRCm38)	splice site	probably benign
R0446:Lyst	UTSW	13	13,638,048 (GRCm38)	missense	probably benign	0.00
R0481:Lyst	UTSW	13	13,677,952 (GRCm38)	missense	probably benign	0.00
R0499:Lyst	UTSW	13	13,616,713 (GRCm38)	missense	probably damaging	1.00
R0506:Lyst	UTSW	13	13,638,015 (GRCm38)	missense	probably benign
R0530:Lyst	UTSW	13	13,757,306 (GRCm38)	splice site	probably benign
R0541:Lyst	UTSW	13	13,681,293 (GRCm38)	missense	probably benign	0.00
R0570:Lyst	UTSW	13	13,709,386 (GRCm38)	missense	probably benign	0.26
R0680:Lyst	UTSW	13	13,650,341 (GRCm38)	missense	probably benign	0.01
R0842:Lyst	UTSW	13	13,678,241 (GRCm38)	nonsense	probably null
R0848:Lyst	UTSW	13	13,634,930 (GRCm38)	missense	probably benign	0.00
R1014:Lyst	UTSW	13	13,634,060 (GRCm38)	missense	possibly damaging	0.49
R1205:Lyst	UTSW	13	13,680,202 (GRCm38)	missense	probably benign
R1251:Lyst	UTSW	13	13,634,483 (GRCm38)	missense	probably benign	0.00
R1304:Lyst	UTSW	13	13,751,984 (GRCm38)	nonsense	probably null
R1398:Lyst	UTSW	13	13,740,536 (GRCm38)	missense	possibly damaging	0.91
R1445:Lyst	UTSW	13	13,640,054 (GRCm38)	missense	possibly damaging	0.94
R1475:Lyst	UTSW	13	13,708,212 (GRCm38)	critical splice donor site	probably null
R1479:Lyst	UTSW	13	13,634,482 (GRCm38)	missense	probably benign	0.00
R1484:Lyst	UTSW	13	13,678,190 (GRCm38)	missense	probably benign	0.01
R1498:Lyst	UTSW	13	13,650,375 (GRCm38)	missense	possibly damaging	0.49
R1540:Lyst	UTSW	13	13,635,101 (GRCm38)	missense	possibly damaging	0.81
R1611:Lyst	UTSW	13	13,634,897 (GRCm38)	missense	probably damaging	0.97
R1653:Lyst	UTSW	13	13,635,226 (GRCm38)	missense	probably damaging	1.00
R1669:Lyst	UTSW	13	13,644,087 (GRCm38)	missense	possibly damaging	0.90
R1686:Lyst	UTSW	13	13,634,705 (GRCm38)	missense	possibly damaging	0.81
R1694:Lyst	UTSW	13	13,661,161 (GRCm38)	missense	probably damaging	0.98
R1747:Lyst	UTSW	13	13,757,422 (GRCm38)	missense	probably benign	0.00
R1793:Lyst	UTSW	13	13,647,083 (GRCm38)	nonsense	probably null
R1871:Lyst	UTSW	13	13,651,712 (GRCm38)	missense	probably benign	0.00
R1905:Lyst	UTSW	13	13,634,134 (GRCm38)	missense	probably benign
R1958:Lyst	UTSW	13	13,616,618 (GRCm38)	missense	probably damaging	1.00
R1969:Lyst	UTSW	13	13,730,344 (GRCm38)	missense	probably damaging	0.99
R2040:Lyst	UTSW	13	13,641,222 (GRCm38)	missense	probably benign	0.00
R2109:Lyst	UTSW	13	13,712,820 (GRCm38)	missense	possibly damaging	0.46
R2116:Lyst	UTSW	13	13,635,701 (GRCm38)	missense	probably damaging	0.99
R2121:Lyst	UTSW	13	13,660,971 (GRCm38)	missense	probably damaging	1.00
R2127:Lyst	UTSW	13	13,635,262 (GRCm38)	missense	probably damaging	1.00
R2187:Lyst	UTSW	13	13,709,341 (GRCm38)	missense	possibly damaging	0.61
R2238:Lyst	UTSW	13	13,743,263 (GRCm38)	missense	probably benign	0.41
R2258:Lyst	UTSW	13	13,637,658 (GRCm38)	missense	probably benign	0.00
R2292:Lyst	UTSW	13	13,740,495 (GRCm38)	missense	probably damaging	1.00
R2368:Lyst	UTSW	13	13,696,663 (GRCm38)	missense	probably damaging	0.96
R2908:Lyst	UTSW	13	13,669,873 (GRCm38)	missense	probably benign	0.03
R3001:Lyst	UTSW	13	13,696,705 (GRCm38)	missense	probably benign
R3002:Lyst	UTSW	13	13,696,705 (GRCm38)	missense	probably benign
R3024:Lyst	UTSW	13	13,658,687 (GRCm38)	missense	probably benign
R3113:Lyst	UTSW	13	13,669,927 (GRCm38)	missense	probably benign	0.12
R3406:Lyst	UTSW	13	13,635,230 (GRCm38)	missense	possibly damaging	0.56
R3972:Lyst	UTSW	13	13,706,625 (GRCm38)	missense	possibly damaging	0.67
R3978:Lyst	UTSW	13	13,634,168 (GRCm38)	missense	possibly damaging	0.82
R4032:Lyst	UTSW	13	13,616,665 (GRCm38)	missense	probably damaging	1.00
R4192:Lyst	UTSW	13	13,740,513 (GRCm38)	missense	probably damaging	1.00
R4206:Lyst	UTSW	13	13,635,989 (GRCm38)	missense	probably benign	0.03
R4298:Lyst	UTSW	13	13,634,887 (GRCm38)	missense	probably damaging	1.00
R4344:Lyst	UTSW	13	13,698,466 (GRCm38)	missense	probably benign	0.06
R4441:Lyst	UTSW	13	13,635,383 (GRCm38)	missense	probably damaging	1.00
R4445:Lyst	UTSW	13	13,709,564 (GRCm38)	missense	probably benign	0.42
R4477:Lyst	UTSW	13	13,635,383 (GRCm38)	missense	probably damaging	1.00
R4493:Lyst	UTSW	13	13,635,383 (GRCm38)	missense	probably damaging	1.00
R4494:Lyst	UTSW	13	13,635,383 (GRCm38)	missense	probably damaging	1.00
R4495:Lyst	UTSW	13	13,635,383 (GRCm38)	missense	probably damaging	1.00
R4622:Lyst	UTSW	13	13,674,398 (GRCm38)	missense	probably benign	0.01
R4638:Lyst	UTSW	13	13,696,794 (GRCm38)	splice site	probably null
R4658:Lyst	UTSW	13	13,635,383 (GRCm38)	missense	probably damaging	1.00
R4675:Lyst	UTSW	13	13,635,383 (GRCm38)	missense	probably damaging	1.00
R4719:Lyst	UTSW	13	13,650,350 (GRCm38)	missense	probably benign
R4729:Lyst	UTSW	13	13,637,901 (GRCm38)	missense	probably damaging	1.00
R4774:Lyst	UTSW	13	13,740,597 (GRCm38)	missense	probably damaging	1.00
R4811:Lyst	UTSW	13	13,777,100 (GRCm38)	missense	probably benign	0.33
R4877:Lyst	UTSW	13	13,683,149 (GRCm38)	missense	probably damaging	1.00
R4920:Lyst	UTSW	13	13,647,060 (GRCm38)	missense	possibly damaging	0.79
R4933:Lyst	UTSW	13	13,759,378 (GRCm38)	missense	probably benign	0.12
R4933:Lyst	UTSW	13	13,637,764 (GRCm38)	missense	probably damaging	0.98
R4958:Lyst	UTSW	13	13,635,463 (GRCm38)	missense	probably benign	0.00
R4982:Lyst	UTSW	13	13,725,954 (GRCm38)	missense	probably damaging	1.00
R4992:Lyst	UTSW	13	13,661,163 (GRCm38)	missense	probably damaging	1.00
R5024:Lyst	UTSW	13	13,634,404 (GRCm38)	missense	probably benign
R5049:Lyst	UTSW	13	13,636,064 (GRCm38)	missense	probably damaging	1.00
R5079:Lyst	UTSW	13	13,757,353 (GRCm38)	missense	probably benign	0.08
R5254:Lyst	UTSW	13	13,683,070 (GRCm38)	missense	probably benign	0.00
R5266:Lyst	UTSW	13	13,660,970 (GRCm38)	missense	probably damaging	1.00
R5279:Lyst	UTSW	13	13,648,802 (GRCm38)	nonsense	probably null
R5285:Lyst	UTSW	13	13,634,426 (GRCm38)	missense	probably benign	0.01
R5435:Lyst	UTSW	13	13,777,064 (GRCm38)	missense	possibly damaging	0.64
R5516:Lyst	UTSW	13	13,644,122 (GRCm38)	missense	probably benign	0.10
R5524:Lyst	UTSW	13	13,746,779 (GRCm38)	missense	probably benign	0.03
R5591:Lyst	UTSW	13	13,743,333 (GRCm38)	missense	probably damaging	0.99
R5592:Lyst	UTSW	13	13,743,333 (GRCm38)	missense	probably damaging	0.99
R5593:Lyst	UTSW	13	13,743,333 (GRCm38)	missense	probably damaging	0.99
R5594:Lyst	UTSW	13	13,743,333 (GRCm38)	missense	probably damaging	0.99
R5594:Lyst	UTSW	13	13,759,397 (GRCm38)	missense	probably benign	0.00
R5644:Lyst	UTSW	13	13,637,496 (GRCm38)	missense	possibly damaging	0.58
R5659:Lyst	UTSW	13	13,634,627 (GRCm38)	missense	possibly damaging	0.58
R5741:Lyst	UTSW	13	13,634,030 (GRCm38)	missense	probably benign	0.44
R5908:Lyst	UTSW	13	13,696,761 (GRCm38)	nonsense	probably null
R5969:Lyst	UTSW	13	13,687,813 (GRCm38)	splice site	probably null
R6128:Lyst	UTSW	13	13,759,379 (GRCm38)	missense	possibly damaging	0.67
R6271:Lyst	UTSW	13	13,658,754 (GRCm38)	missense	probably benign	0.30
R6315:Lyst	UTSW	13	13,643,504 (GRCm38)	missense	probably benign
R6318:Lyst	UTSW	13	13,743,311 (GRCm38)	missense	possibly damaging	0.88
R6555:Lyst	UTSW	13	13,648,925 (GRCm38)	missense	probably benign	0.01
R6663:Lyst	UTSW	13	13,664,116 (GRCm38)	splice site	probably null
R6701:Lyst	UTSW	13	13,681,485 (GRCm38)	missense	probably benign	0.06
R6711:Lyst	UTSW	13	13,635,235 (GRCm38)	missense	possibly damaging	0.80
R6909:Lyst	UTSW	13	13,743,375 (GRCm38)	missense	probably damaging	1.00
R6915:Lyst	UTSW	13	13,726,044 (GRCm38)	missense	probably benign	0.01
R6929:Lyst	UTSW	13	13,743,324 (GRCm38)	missense	probably damaging	1.00
R6960:Lyst	UTSW	13	13,634,078 (GRCm38)	missense	probably benign	0.12
R7018:Lyst	UTSW	13	13,743,459 (GRCm38)	critical splice donor site	probably null
R7037:Lyst	UTSW	13	13,616,666 (GRCm38)	missense	probably damaging	1.00
R7045:Lyst	UTSW	13	13,637,708 (GRCm38)	missense	probably damaging	1.00
R7045:Lyst	UTSW	13	13,634,900 (GRCm38)	missense	probably benign	0.34
R7070:Lyst	UTSW	13	13,757,444 (GRCm38)	missense	probably benign	0.23
R7188:Lyst	UTSW	13	13,752,090 (GRCm38)	missense	possibly damaging	0.66
R7201:Lyst	UTSW	13	13,709,300 (GRCm38)	nonsense	probably null
R7210:Lyst	UTSW	13	13,656,983 (GRCm38)	missense	probably damaging	1.00
R7229:Lyst	UTSW	13	13,643,509 (GRCm38)	missense	probably benign	0.00
R7293:Lyst	UTSW	13	13,680,237 (GRCm38)	missense	probably benign	0.01
R7318:Lyst	UTSW	13	13,757,443 (GRCm38)	missense	probably benign	0.13
R7344:Lyst	UTSW	13	13,706,555 (GRCm38)	missense	probably benign
R7426:Lyst	UTSW	13	13,637,524 (GRCm38)	missense	probably benign
R7522:Lyst	UTSW	13	13,647,083 (GRCm38)	nonsense	probably null
R7583:Lyst	UTSW	13	13,635,887 (GRCm38)	missense	probably damaging	1.00
R7606:Lyst	UTSW	13	13,637,475 (GRCm38)	missense	probably damaging	1.00
R7636:Lyst	UTSW	13	13,616,747 (GRCm38)	critical splice donor site	probably null
R7658:Lyst	UTSW	13	13,730,476 (GRCm38)	missense	possibly damaging	0.63
R7685:Lyst	UTSW	13	13,669,865 (GRCm38)	missense	probably benign	0.00
R7689:Lyst	UTSW	13	13,683,223 (GRCm38)	critical splice donor site	probably null
R7765:Lyst	UTSW	13	13,709,532 (GRCm38)	missense	possibly damaging	0.75
R7779:Lyst	UTSW	13	13,634,543 (GRCm38)	missense	probably damaging	1.00
R7871:Lyst	UTSW	13	13,636,052 (GRCm38)	nonsense	probably null
R7872:Lyst	UTSW	13	13,635,865 (GRCm38)	missense	probably benign	0.14
R7884:Lyst	UTSW	13	13,707,683 (GRCm38)	missense	probably benign	0.09
R7890:Lyst	UTSW	13	13,740,569 (GRCm38)	missense	probably damaging	0.99
R7916:Lyst	UTSW	13	13,647,072 (GRCm38)	missense	possibly damaging	0.64
R7948:Lyst	UTSW	13	13,746,589 (GRCm38)	missense	possibly damaging	0.59
R7956:Lyst	UTSW	13	13,641,203 (GRCm38)	missense	possibly damaging	0.80
R8048:Lyst	UTSW	13	13,687,645 (GRCm38)	missense	probably benign	0.12
R8085:Lyst	UTSW	13	13,634,309 (GRCm38)	missense	probably damaging	0.98
R8165:Lyst	UTSW	13	13,698,360 (GRCm38)	missense	probably damaging	0.99
R8235:Lyst	UTSW	13	13,760,738 (GRCm38)	missense	possibly damaging	0.69
R8237:Lyst	UTSW	13	13,651,732 (GRCm38)	missense	probably benign	0.00
R8275:Lyst	UTSW	13	13,776,082 (GRCm38)	missense	probably benign	0.02
R8300:Lyst	UTSW	13	13,664,058 (GRCm38)	missense	possibly damaging	0.79
R8350:Lyst	UTSW	13	13,650,388 (GRCm38)	nonsense	probably null
R8526:Lyst	UTSW	13	13,760,806 (GRCm38)	missense	probably damaging	0.99
R8551:Lyst	UTSW	13	13,634,060 (GRCm38)	missense	possibly damaging	0.77
R8723:Lyst	UTSW	13	13,712,757 (GRCm38)	missense	possibly damaging	0.89
R8772:Lyst	UTSW	13	13,637,492 (GRCm38)	nonsense	probably null
R8778:Lyst	UTSW	13	13,728,567 (GRCm38)	missense	possibly damaging	0.89
R8778:Lyst	UTSW	13	13,635,776 (GRCm38)	missense	possibly damaging	0.89
R8801:Lyst	UTSW	13	13,661,010 (GRCm38)	missense	probably benign	0.10
R8837:Lyst	UTSW	13	13,677,963 (GRCm38)	missense	probably benign
R8874:Lyst	UTSW	13	13,637,562 (GRCm38)	missense	probably benign
R8878:Lyst	UTSW	13	13,641,076 (GRCm38)	missense	probably benign	0.00
R8891:Lyst	UTSW	13	13,712,850 (GRCm38)	missense	possibly damaging	0.67
R9077:Lyst	UTSW	13	13,683,108 (GRCm38)	missense	probably benign	0.02
R9127:Lyst	UTSW	13	13,634,242 (GRCm38)	missense	probably damaging	1.00
R9143:Lyst	UTSW	13	13,661,165 (GRCm38)	missense	probably damaging	0.98
R9216:Lyst	UTSW	13	13,648,603 (GRCm38)	missense	probably benign
R9217:Lyst	UTSW	13	13,696,660 (GRCm38)	missense	probably benign	0.01
R9291:Lyst	UTSW	13	13,709,353 (GRCm38)	missense	probably benign	0.01
R9302:Lyst	UTSW	13	13,730,362 (GRCm38)	missense	possibly damaging	0.46
R9370:Lyst	UTSW	13	13,760,748 (GRCm38)	missense	probably damaging	1.00
R9402:Lyst	UTSW	13	13,637,878 (GRCm38)	missense	probably benign
R9457:Lyst	UTSW	13	13,687,745 (GRCm38)	missense	possibly damaging	0.83
R9481:Lyst	UTSW	13	13,683,068 (GRCm38)	missense	possibly damaging	0.68
R9563:Lyst	UTSW	13	13,637,823 (GRCm38)	missense	probably benign	0.36
R9623:Lyst	UTSW	13	13,678,002 (GRCm38)	missense	probably benign
R9661:Lyst	UTSW	13	13,634,194 (GRCm38)	missense	probably benign	0.01
R9682:Lyst	UTSW	13	13,656,941 (GRCm38)	missense	probably benign	0.21
R9743:Lyst	UTSW	13	13,634,738 (GRCm38)	missense	possibly damaging	0.67
R9801:Lyst	UTSW	13	13,634,705 (GRCm38)	missense	probably damaging	0.97
RF001:Lyst	UTSW	13	13,635,841 (GRCm38)	missense	probably benign
RF002:Lyst	UTSW	13	13,634,363 (GRCm38)	missense	probably benign	0.05
X0024:Lyst	UTSW	13	13,634,448 (GRCm38)	missense	probably benign	0.00
X0026:Lyst	UTSW	13	13,751,970 (GRCm38)	missense	probably damaging	0.99
Z1088:Lyst	UTSW	13	13,743,433 (GRCm38)	missense	probably benign	0.09
Z1176:Lyst	UTSW	13	13,777,079 (GRCm38)	missense	probably benign	0.27
Z1176:Lyst	UTSW	13	13,640,107 (GRCm38)	missense	probably damaging	1.00
Z1177:Lyst	UTSW	13	13,680,134 (GRCm38)	missense	possibly damaging	0.73

Predicted Primers

PCR Primer
(F):5'- AGGTATTGTCTGCTGCCTGC -3'
(R):5'- AGACCAGGATTGCTCTCAGG -3'

Sequencing Primer
(F):5'- TGCCTGCACCTCTGACCAAG -3'
(R):5'- CAGGATTGCTCTCAGGTTAATTTATG -3'

Posted On

2016-08-04