Incidental Mutation 'R5338:Aftph'
ID 423643
Institutional Source Beutler Lab
Gene Symbol Aftph
Ensembl Gene ENSMUSG00000049659
Gene Name aftiphilin
Synonyms 9130023F12Rik
Accession Numbers
Essential gene? Probably essential (E-score: 0.835) question?
Stock # R5338 (G1)
Quality Score 225
Status Not validated
Chromosome 11
Chromosomal Location 20635084-20691589 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to T at 20677203 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Aspartic acid to Glutamic Acid at position 135 (D135E)
Ref Sequence ENSEMBL: ENSMUSP00000134991 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000035350] [ENSMUST00000146722] [ENSMUST00000177014] [ENSMUST00000177543]
AlphaFold Q80WT5
Predicted Effect probably benign
Transcript: ENSMUST00000035350
AA Change: D135E

PolyPhen 2 Score 0.055 (Sensitivity: 0.94; Specificity: 0.84)
SMART Domains Protein: ENSMUSP00000036778
Gene: ENSMUSG00000049659
AA Change: D135E

DomainStartEndE-ValueType
low complexity region 19 45 N/A INTRINSIC
low complexity region 546 560 N/A INTRINSIC
low complexity region 618 631 N/A INTRINSIC
Pfam:Clathrin_bdg 693 765 1.1e-36 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000120008
Predicted Effect probably benign
Transcript: ENSMUST00000146722
AA Change: D135E

PolyPhen 2 Score 0.055 (Sensitivity: 0.94; Specificity: 0.84)
SMART Domains Protein: ENSMUSP00000121612
Gene: ENSMUSG00000049659
AA Change: D135E

DomainStartEndE-ValueType
low complexity region 19 45 N/A INTRINSIC
low complexity region 546 560 N/A INTRINSIC
low complexity region 618 631 N/A INTRINSIC
Pfam:Clathrin_bdg 694 765 1.2e-23 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000176782
SMART Domains Protein: ENSMUSP00000134791
Gene: ENSMUSG00000049659

DomainStartEndE-ValueType
low complexity region 68 82 N/A INTRINSIC
low complexity region 140 153 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000177014
SMART Domains Protein: ENSMUSP00000135450
Gene: ENSMUSG00000049659

DomainStartEndE-ValueType
low complexity region 391 405 N/A INTRINSIC
low complexity region 463 476 N/A INTRINSIC
Pfam:Clathrin_bdg 538 610 6.8e-37 PFAM
low complexity region 664 677 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000177543
AA Change: D135E

PolyPhen 2 Score 0.177 (Sensitivity: 0.92; Specificity: 0.87)
SMART Domains Protein: ENSMUSP00000134991
Gene: ENSMUSG00000049659
AA Change: D135E

DomainStartEndE-ValueType
low complexity region 19 45 N/A INTRINSIC
low complexity region 546 560 N/A INTRINSIC
low complexity region 618 631 N/A INTRINSIC
Pfam:Clathrin_bdg 693 765 8.7e-37 PFAM
Coding Region Coverage
  • 1x: 99.3%
  • 3x: 98.7%
  • 10x: 97.5%
  • 20x: 95.8%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 67 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
A4gnt T A 9: 99,502,597 (GRCm39) N252K probably damaging Het
Abhd16a A G 17: 35,313,278 (GRCm39) E132G probably damaging Het
Adgrv1 G A 13: 81,677,165 (GRCm39) R1889W possibly damaging Het
Arf1 A C 11: 59,104,041 (GRCm39) F63V probably damaging Het
Atg14 G A 14: 47,805,656 (GRCm39) R70C probably damaging Het
B3gnt7 T A 1: 86,233,366 (GRCm39) F87Y probably damaging Het
Bcat1 A G 6: 144,953,353 (GRCm39) Y345H possibly damaging Het
Birc2 G T 9: 7,857,360 (GRCm39) T371K probably benign Het
Ccdc175 A T 12: 72,231,745 (GRCm39) S5T probably damaging Het
Ccdc186 A T 19: 56,801,689 (GRCm39) S143T possibly damaging Het
Cct6b A T 11: 82,653,015 (GRCm39) M28K possibly damaging Het
Cers4 C A 8: 4,565,680 (GRCm39) A42D probably damaging Het
Crmp1 G T 5: 37,437,018 (GRCm39) V236L probably benign Het
Ctdspl2 T A 2: 121,811,793 (GRCm39) N212K probably benign Het
Dclk3 T C 9: 111,298,127 (GRCm39) V557A possibly damaging Het
Dnmt1 A T 9: 20,864,015 (GRCm39) H23Q probably benign Het
Dpp8 G T 9: 64,971,206 (GRCm39) E609* probably null Het
Dtx3 C A 10: 127,028,919 (GRCm39) M106I probably benign Het
Ero1b A C 13: 12,589,732 (GRCm39) D45A probably damaging Het
Fcho2 T A 13: 98,867,399 (GRCm39) D688V probably damaging Het
Fhod3 A T 18: 25,161,138 (GRCm39) S557C probably damaging Het
Flnc T C 6: 29,444,063 (GRCm39) S626P possibly damaging Het
Foxi3 G T 6: 70,937,602 (GRCm39) G278V probably damaging Het
Gbp10 A G 5: 105,372,166 (GRCm39) L198P probably damaging Het
Gcn1 A G 5: 115,721,462 (GRCm39) T458A probably benign Het
Gigyf1 C T 5: 137,521,422 (GRCm39) probably benign Het
Gtpbp2 A C 17: 46,478,760 (GRCm39) T526P probably damaging Het
Gys2 A G 6: 142,400,239 (GRCm39) L324S probably damaging Het
Hpn C A 7: 30,802,781 (GRCm39) V119L probably benign Het
Ino80d C A 1: 63,098,098 (GRCm39) V599L probably benign Het
Kcnh3 G T 15: 99,140,275 (GRCm39) G1054* probably null Het
Maml1 A T 11: 50,157,778 (GRCm39) D132E probably benign Het
Mastl A T 2: 23,023,503 (GRCm39) S407T probably benign Het
Mettl1 T C 10: 126,878,954 (GRCm39) W3R probably damaging Het
Mprip A G 11: 59,651,399 (GRCm39) Y1701C probably damaging Het
Mylk3 T C 8: 86,069,350 (GRCm39) N484D probably damaging Het
Nod2 G A 8: 89,399,413 (GRCm39) probably null Het
Nrde2 A T 12: 100,097,037 (GRCm39) F921Y probably damaging Het
Nup88 G T 11: 70,835,734 (GRCm39) T575N probably damaging Het
Nxpe3 T C 16: 55,686,706 (GRCm39) T101A possibly damaging Het
Or1o3 T A 17: 37,574,532 (GRCm39) T8S probably benign Het
Or2r11 A G 6: 42,437,908 (GRCm39) M15T probably benign Het
Or4c109 C T 2: 88,817,809 (GRCm39) V246I possibly damaging Het
Or52e15 A G 7: 104,645,514 (GRCm39) L199S possibly damaging Het
Or8g32 A T 9: 39,305,371 (GRCm39) I92F probably damaging Het
Pcnx3 A T 19: 5,722,624 (GRCm39) L1356Q probably damaging Het
Pkd1 A T 17: 24,813,510 (GRCm39) M4017L probably benign Het
Polg2 A G 11: 106,670,064 (GRCm39) C69R possibly damaging Het
Prr5l T G 2: 101,547,452 (GRCm39) S358R probably benign Het
Ptprg A G 14: 12,154,111 (GRCm38) T611A probably benign Het
Ryk G T 9: 102,774,516 (GRCm39) E417* probably null Het
Sbp T C 17: 24,161,396 (GRCm39) probably benign Het
Sema4a T C 3: 88,358,804 (GRCm39) T155A probably benign Het
Shank3 A G 15: 89,415,914 (GRCm39) probably null Het
Slc35d2 A T 13: 64,245,496 (GRCm39) M294K possibly damaging Het
Slc41a3 A T 6: 90,589,153 (GRCm39) N88I possibly damaging Het
Slit3 A G 11: 35,512,975 (GRCm39) T534A probably benign Het
Ssx2ip A G 3: 146,142,296 (GRCm39) probably null Het
Stard13 T C 5: 150,983,063 (GRCm39) K648R probably damaging Het
Tanc1 A T 2: 59,626,178 (GRCm39) T512S probably damaging Het
Tbcc T C 17: 47,202,082 (GRCm39) V156A probably benign Het
Terf1 T G 1: 15,901,787 (GRCm39) I273R possibly damaging Het
Tesc A G 5: 118,197,523 (GRCm39) Y179C probably damaging Het
Ttc3 T G 16: 94,184,900 (GRCm39) V40G probably damaging Het
Tysnd1 T A 10: 61,532,028 (GRCm39) F227I probably damaging Het
Vps53 T C 11: 75,972,034 (GRCm39) E271G probably damaging Het
Wdfy4 C T 14: 32,812,823 (GRCm39) R1650H probably damaging Het
Other mutations in Aftph
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00952:Aftph APN 11 20,677,483 (GRCm39) missense probably damaging 1.00
IGL01485:Aftph APN 11 20,642,507 (GRCm39) missense probably damaging 1.00
IGL01622:Aftph APN 11 20,659,632 (GRCm39) missense probably damaging 1.00
IGL01623:Aftph APN 11 20,659,632 (GRCm39) missense probably damaging 1.00
IGL01739:Aftph APN 11 20,676,994 (GRCm39) missense probably damaging 0.98
IGL01777:Aftph APN 11 20,676,554 (GRCm39) missense possibly damaging 0.49
IGL01825:Aftph APN 11 20,676,569 (GRCm39) missense possibly damaging 0.77
IGL02804:Aftph APN 11 20,676,107 (GRCm39) missense possibly damaging 0.89
IGL03003:Aftph APN 11 20,676,982 (GRCm39) nonsense probably null
IGL03111:Aftph APN 11 20,676,040 (GRCm39) missense probably benign 0.44
R1103:Aftph UTSW 11 20,676,547 (GRCm39) missense probably benign 0.00
R1672:Aftph UTSW 11 20,676,762 (GRCm39) missense probably benign 0.10
R2060:Aftph UTSW 11 20,642,571 (GRCm39) missense probably damaging 1.00
R2141:Aftph UTSW 11 20,648,318 (GRCm39) nonsense probably null
R2241:Aftph UTSW 11 20,676,328 (GRCm39) missense possibly damaging 0.90
R2513:Aftph UTSW 11 20,658,676 (GRCm39) splice site probably null
R2518:Aftph UTSW 11 20,675,797 (GRCm39) missense probably damaging 0.98
R4751:Aftph UTSW 11 20,677,074 (GRCm39) missense probably damaging 1.00
R4844:Aftph UTSW 11 20,658,667 (GRCm39) splice site probably benign
R4879:Aftph UTSW 11 20,648,311 (GRCm39) critical splice donor site probably null
R4895:Aftph UTSW 11 20,646,801 (GRCm39) missense probably damaging 1.00
R5012:Aftph UTSW 11 20,648,264 (GRCm39) intron probably benign
R5082:Aftph UTSW 11 20,677,100 (GRCm39) missense probably damaging 0.98
R5093:Aftph UTSW 11 20,659,619 (GRCm39) critical splice donor site probably null
R5160:Aftph UTSW 11 20,662,197 (GRCm39) missense probably benign 0.03
R5288:Aftph UTSW 11 20,676,994 (GRCm39) missense probably damaging 0.98
R5794:Aftph UTSW 11 20,676,955 (GRCm39) splice site probably null
R6747:Aftph UTSW 11 20,676,144 (GRCm39) splice site probably null
R6876:Aftph UTSW 11 20,659,744 (GRCm39) missense probably damaging 1.00
R7034:Aftph UTSW 11 20,642,498 (GRCm39) missense probably damaging 1.00
R7284:Aftph UTSW 11 20,676,812 (GRCm39) missense probably benign 0.00
R7371:Aftph UTSW 11 20,676,836 (GRCm39) missense probably benign
R7974:Aftph UTSW 11 20,648,233 (GRCm39) makesense probably null
R8290:Aftph UTSW 11 20,675,712 (GRCm39) missense probably benign 0.01
X0028:Aftph UTSW 11 20,676,338 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- AGGTCATCTGTTCCCTGAGG -3'
(R):5'- CGTCCCAAGGAAGACTTTGTAC -3'

Sequencing Primer
(F):5'- CCTGAGGATTTACAGTTTCCAAACC -3'
(R):5'- GGAAGACTTTGTACCTTCAAACC -3'
Posted On 2016-08-04